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Protein

Cleavage stimulation factor subunit 2 tau variant

Gene

Cstf2t

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs.1 Publication

GO - Molecular functioni

  • mRNA binding Source: GO_Central
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI

GO - Biological processi

  • mRNA polyadenylation Source: MGI
  • pre-mRNA cleavage required for polyadenylation Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 2 tau variant
Alternative name(s):
CF-1 64 kDa subunit tau variant
Cleavage stimulation factor 64 kDa subunit tau variant
Short name:
CSTF 64 kDa subunit tau variant
TauCstF-64
Gene namesi
Name:Cstf2t
Synonyms:Kiaa0689
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1932622. Cstf2t.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 632632Cleavage stimulation factor subunit 2 tau variantPRO_0000081535Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei579 – 5791PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8C7E9.
MaxQBiQ8C7E9.
PaxDbiQ8C7E9.
PeptideAtlasiQ8C7E9.
PRIDEiQ8C7E9.

2D gel databases

REPRODUCTION-2DPAGEIPI00467932.

PTM databases

iPTMnetiQ8C7E9.
PhosphoSiteiQ8C7E9.

Expressioni

Tissue specificityi

Expressed in testes, where it is restricted to pachytene spermatocytes and spermatids, and in the brain (at protein level).1 Publication

Gene expression databases

BgeeiQ8C7E9.
GenevisibleiQ8C7E9. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8C7E9. 1 interaction.
MINTiMINT-4110556.
STRINGi10090.ENSMUSP00000093831.

Structurei

3D structure databases

ProteinModelPortaliQ8C7E9.
SMRiQ8C7E9. Positions 8-111, 584-631.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 9479RRMPROSITE-ProRule annotationAdd
BLAST
Repeati428 – 43251-1; approximate
Repeati433 – 43751-2; approximate
Repeati438 – 44251-3; approximate
Repeati443 – 44641-4; approximate
Repeati447 – 45151-5; approximate
Repeati452 – 45651-6
Repeati457 – 46151-7; approximate
Repeati462 – 46651-8; approximate
Repeati508 – 51252-1; approximate
Repeati513 – 51752-2
Repeati518 – 52252-3; approximate
Repeati523 – 52752-4
Repeati528 – 53252-5; approximate
Repeati533 – 53752-6
Repeati538 – 54252-7; approximate
Repeati543 – 54752-8
Repeati548 – 55142-9; approximate
Repeati552 – 55652-10; approximate
Repeati557 – 56042-11; approximate
Repeati561 – 56552-12

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni428 – 466398 X 5 AA tandem repeats of M-E-T-R-[AG]Add
BLAST
Regioni508 – 5655812 X 5 AA tandem repeats of G-[AT]-G-[MI]-QAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi271 – 572302Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XZHK. LUCA.
GeneTreeiENSGT00820000127074.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiQ8C7E9.
KOiK14407.
OMAiMHHASGH.
OrthoDBiEOG7N63NZ.
PhylomeDBiQ8C7E9.
TreeFamiTF314948.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C7E9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLAVRDPA MDRSLRSVFV GNIPYEATEE QLKDIFSEVG SVVSFRLVYD
60 70 80 90 100
RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE
110 120 130 140 150
LKSLGPAAPI IDSPYGDPID PEDAPESITR AVASLPPEQM FELMKQMKLC
160 170 180 190 200
VQNSHQEARN MLLQNPQLAY ALLQAQVVMR IMDPEIALKI LHRKIHVTPL
210 220 230 240 250
IPGKSQPVSG PGPGGPGPSG PGGPGPGPAP GLCPGPNVML NQQNPPAPQP
260 270 280 290 300
QHLPRRPVKD IPPLMQTSIQ GGIPAPGPIP AAVPGPGPGS LTPGGAMQPQ
310 320 330 340 350
VGMPVVGPVP LERGQMQISD PRPPMPRGPM PSGGIPPRGL LGDAPNDPRG
360 370 380 390 400
GTLLSVTGEV EPRGYMGPPH QGPPMHHGHD NRGPASHDMR GGPLAADPRM
410 420 430 440 450
LIGEPRGPMI DQRGLPMDGR GGRESRGMET RPMETEVLEP RGMERRMETC
460 470 480 490 500
AMETRGMDAR GLEMRGPGPS SRGPMTGGIQ GPGPINMGAG GPQGPRQVPN
510 520 530 540 550
IAGVGNPGGT MQGAGIQGGG MQGAGMQGGG MQGAGMQGGG MQGAGMQAGM
560 570 580 590 600
QGASMQGGMQ GAGMQGASKQ GGGQPSSFSP GQSQVTPQDQ EKAALIMQVL
610 620 630
QLTADQIAML PPEQRQSILI LKEQIQKSTG AS
Length:632
Mass (Da):65,862
Last modified:July 19, 2005 - v2
Checksum:i1CDB48EEC5B814B8
GO

Sequence cautioni

The sequence BAC39256.1 differs from that shown. Reason: Frameshift at position 583. Curated
The sequence BAD32280.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti213 – 22715PGGPG…GPGPG → LVGWASGLAAGPA (PubMed:11113135).CuratedAdd
BLAST
Sequence conflicti214 – 2163GGP → WWAL in BAC39256 (PubMed:16141072).Curated
Sequence conflicti221 – 2211P → S in BAC34240 (PubMed:16141072).Curated
Sequence conflicti457 – 4571M → MEARGM in AAH26995 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322194 mRNA. Translation: AAG40327.1.
AK173002 mRNA. Translation: BAD32280.1. Different initiation.
AK034853 mRNA. Translation: BAC28855.1.
AK050407 mRNA. Translation: BAC34240.1.
AK082910 mRNA. Translation: BAC38683.1.
AK084696 mRNA. Translation: BAC39256.1. Frameshift.
BC026995 mRNA. Translation: AAH26995.1.
CCDSiCCDS37958.1.
RefSeqiNP_112539.2. NM_031249.2.
UniGeneiMm.22031.

Genome annotation databases

EnsembliENSMUST00000066039; ENSMUSP00000093831; ENSMUSG00000053536.
GeneIDi83410.
KEGGimmu:83410.
UCSCiuc008her.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322194 mRNA. Translation: AAG40327.1.
AK173002 mRNA. Translation: BAD32280.1. Different initiation.
AK034853 mRNA. Translation: BAC28855.1.
AK050407 mRNA. Translation: BAC34240.1.
AK082910 mRNA. Translation: BAC38683.1.
AK084696 mRNA. Translation: BAC39256.1. Frameshift.
BC026995 mRNA. Translation: AAH26995.1.
CCDSiCCDS37958.1.
RefSeqiNP_112539.2. NM_031249.2.
UniGeneiMm.22031.

3D structure databases

ProteinModelPortaliQ8C7E9.
SMRiQ8C7E9. Positions 8-111, 584-631.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8C7E9. 1 interaction.
MINTiMINT-4110556.
STRINGi10090.ENSMUSP00000093831.

PTM databases

iPTMnetiQ8C7E9.
PhosphoSiteiQ8C7E9.

2D gel databases

REPRODUCTION-2DPAGEIPI00467932.

Proteomic databases

EPDiQ8C7E9.
MaxQBiQ8C7E9.
PaxDbiQ8C7E9.
PeptideAtlasiQ8C7E9.
PRIDEiQ8C7E9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066039; ENSMUSP00000093831; ENSMUSG00000053536.
GeneIDi83410.
KEGGimmu:83410.
UCSCiuc008her.1. mouse.

Organism-specific databases

CTDi23283.
MGIiMGI:1932622. Cstf2t.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XZHK. LUCA.
GeneTreeiENSGT00820000127074.
HOGENOMiHOG000214373.
HOVERGENiHBG051145.
InParanoidiQ8C7E9.
KOiK14407.
OMAiMHHASGH.
OrthoDBiEOG7N63NZ.
PhylomeDBiQ8C7E9.
TreeFamiTF314948.

Miscellaneous databases

PROiQ8C7E9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C7E9.
GenevisibleiQ8C7E9. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gene for a variant form of the polyadenylation protein CstF-64 is on chromosome 19 and is expressed in pachytene spermatocytes in mice."
    Dass B., McMahon K.W., Jenkins N.A., Gilbert D.J., Copeland N.G., MacDonald C.C.
    J. Biol. Chem. 276:8044-8050(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA-BINDING, FUNCTION.
    Strain: CD-1.
    Tissue: Testis.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Heart and Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary tumor.
  5. "Developmental distribution of the polyadenylation protein CstF-64 and the variant tauCstF-64 in mouse and rat testis."
    Wallace A.M., Denison T.L., Attaya E.N., MacDonald C.C.
    Biol. Reprod. 70:1080-1087(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-579, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Testis.

Entry informationi

Entry nameiCSTFT_MOUSE
AccessioniPrimary (citable) accession number: Q8C7E9
Secondary accession number(s): Q6A016
, Q8BHH7, Q8C3W7, Q8R2Y1, Q9EPU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: July 6, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.