Q8C767 (PPR3B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 3B

Alternative name(s):
Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
Protein phosphatase 1 regulatory subunit 4
Short name=PP1 subunit R4
Protein phosphatase 1 subunit GL
Short name=PTG

Gene names

Name:	Ppp1r3b
Synonyms:	Ppp1r4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes By similarity.
Subunit structure	Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity By similarity.
Tissue specificity	Highly expressed in liver (at protein level). Expressed predominantly in liver. Expressed moderately in heart. Expressed weakly in prostate, stomach, thyroid, lung, kidney, spleen and skeletal muscle. Ref.3 Ref.4
Developmental stage	Expressed in lung at 12.5 and 16.5 dpc and declines thereafter. Expressed in epithelial cells of the bronchus and smooth muscle of the pulmonary artery at 13.5 and 16.5 dpc. Ref.4
Induction	Up-regulated by TITF1. Ref.4
Domain	The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL By similarity.
Sequence similarities	Contains 1 CBM21 (carbohydrate binding type-21) domain.
Sequence caution	The sequence AAH60261.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycogen metabolic process Traceable author statement Ref.3. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Protein phosphatase 1 regulatory subunit 3B		PRO_0000324544
Regions
	Domain	124 – 232	109	CBM21
	Motif	61 – 64	4	PP1-binding motif

Sequences

	Sequence		Length	Mass (Da)
	Q8C767 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 193C1BAC1286D344	FASTA	284	32,433
	10 20 30 40 50 60 MAVDIQYSYS SMAPSLRRER FTFKISPKLS KPLRPCIQLG SKDEASGMVA PAVQEKKVKK 70 80 90 100 110 120 RVSFADNQGL ALTMVKVFSE FDDPLDIPFN ITELLDNIVS LTTAESESFV LDFPQPSADY 130 140 150 160 170 180 LDFRNRLQTN HVCLENCVLK DKAIAGTVKV QNLAFEKVVK IRMTFDTWKS FTDFPCQYVK 190 200 210 220 230 240 DTYAGSDRDT FSFDISLPEK IQSYERMEFA VCYECNGQAY WDSNKGKNYR ITRAELRSSP 250 260 270 280 GKIEPYNGPD FGISFDQFGS PRCSFGLFPE WPSYLGYEKL GPYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver." Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W. Diabetes 51:591-598(2002) [PubMed: 11872655] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"Identification and expression of alternative splice variants of the mouse Ppp1r3b gene in lung epithelial cells." Niimi T., Kurotani R., Kimura S., Kitagawa Y. Biochem. Biophys. Res. Commun. 349:588-596(2006) [PubMed: 16949035] [Abstract] Cited for: INDUCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK052438 mRNA. Translation: BAC34990.1. BC060261 mRNA. Translation: AAH60261.1. Different initiation. BC079666 mRNA. Translation: AAH79666.1.
IPI	IPI00453901.
RefSeq	NP_808409.1. NM_177741.3.
UniGene	Mm.247126.
3D structure databases
ProteinModelPortal	Q8C767.
SMR	Q8C767. Positions 109-252.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8C767.
Protein family/group databases
CAZy	CBM21. Carbohydrate-Binding Module Family 21.
Proteomic databases
PRIDE	Q8C767.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000070481; ENSMUSP00000065679; ENSMUSG00000046794.
GeneID	244416.
KEGG	mmu:244416.
UCSC	uc009lkw.1. mouse.
Organism-specific databases
CTD	79660.
MGI	MGI:2177268. Ppp1r3b.
Phylogenomic databases
eggNOG	roNOG15596.
GeneTree	ENSGT00530000062978.
HOGENOM	HBG716629.
HOVERGEN	HBG052744.
InParanoid	Q8C767.
OrthoDB	EOG47PX72.
Gene expression databases
ArrayExpress	Q8C767.
Bgee	Q8C767.
Genevestigator	Q8C767.
Family and domain databases
InterPro	IPR005036. CBM_21. IPR017434. Pase-1_Glycogen_target-su_met. [Graphical view]
KO	K07189.
Pfam	PF03370. CBM_21. 1 hit. [Graphical view]
PIRSF	PIRSF038207. PP1_GT_animal. 1 hit.
PROSITE	PS51159. CBM21. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	386258.
SOURCE	Search...

Entry information

Entry name

PPR3B_MOUSE

Accession

Primary (citable) accession number: Q8C767
Secondary accession number(s): Q6PAJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 1, 2003
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program