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Protein

Zinc finger protein 57

Gene

Zfp57

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions. Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Binds to a 5'-TGCCGC-3' consensus sequence and recognizes the methylated CpG within this element.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei178Crucial for 5-methylcytosine recognition1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri90 – 113C2H2-type 1; degeneratePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri140 – 162C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri168 – 190C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri264 – 286C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri313 – 332C2H2-type 5; degeneratePROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA methylation involved in embryo development Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • regulation of gene expression by genetic imprinting Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 57
Short name:
Zfp-57
Gene namesi
Name:Zfp57
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:99204 Zfp57

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Affects the maintenance of DNA methylation imprints. The absence of just the zygotic function causes partial neonatal lethality, whereas eliminating both the maternal and zygotic functions results in a highly penetrant embryonic lethality. In oocytes, its absence results in failure to establish maternal methylation imprints at the Snrpn imprinted region. Intriguingly, methylation imprints are reacquired specifically at the maternally derived Snrpn imprinted region when the zygotic Zfp57 is present in embryos.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002919651 – 421Zinc finger protein 57Add BLAST421

Proteomic databases

PaxDbiQ8C6P8
PeptideAtlasiQ8C6P8
PRIDEiQ8C6P8

PTM databases

iPTMnetiQ8C6P8
PhosphoSitePlusiQ8C6P8

Expressioni

Tissue specificityi

Expressed in oocytes and in a subset of adult tissues. Expressed at high levels in testis, and at low levels in cerebellum. Present in sciatic nerve and spinal cord (at protein level).2 Publications

Developmental stagei

Expression peaks between E11 and E17, and decreases from P0 to P28. Expressed in lung throughout embryonic development. At E12, expressed in spinal cord, dorsal root ganglia and sciatic nerve. At E15, highly expressed in all neural tissues. At P0, expressed in brain and spinal cord. Present in Schwann cells at E16 and P0 (at protein level). Maternal product is present in preimplantation embryos. Expressed in pluripotent embryonic stem cells. Down-regulated when embryonic stem cells differentiate.4 Publications

Inductioni

Up-regulated by LIF. Down-regulated during differentiation of embryonic stem cells, or during retinoic acid-induced differentiation of F9 cells (at protein level). Regulated by JMJD1A, which mediates histone H3K9Me2 demethylation at its promoter, thereby activating expression.5 Publications

Gene expression databases

BgeeiENSMUSG00000036036
CleanExiMM_ZFP57
ExpressionAtlasiQ8C6P8 baseline and differential
GenevisibleiQ8C6P8 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065811

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni143 – 145Combined sources3
Beta strandi148 – 151Combined sources4
Helixi152 – 163Combined sources12
Turni171 – 173Combined sources3
Beta strandi176 – 179Combined sources4
Helixi180 – 186Combined sources7
Helixi187 – 190Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GZNX-ray0.99C137-195[»]
4M9VX-ray0.97C/F137-195[»]
ProteinModelPortaliQ8C6P8
SMRiQ8C6P8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 88KRABPROSITE-ProRule annotationAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi377 – 383Poly-Arg7

Domaini

The KRAB domain is required for function as transcriptional repressor.
Zinc fingers 2 and 3 mediate recognition of the target element, ZF2 interacting with the 5' half (TGC) and ZF3 interacting with the 3' half (CGC).

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri90 – 113C2H2-type 1; degeneratePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri140 – 162C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri168 – 190C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri264 – 286C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri313 – 332C2H2-type 5; degeneratePROSITE-ProRule annotationAdd BLAST20

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00390000002599
HOGENOMiHOG000155778
HOVERGENiHBG108764
InParanoidiQ8C6P8
KOiK09228
OMAiYLYSHQF
OrthoDBiEOG091G0P6J
PhylomeDBiQ8C6P8
TreeFamiTF337947

Family and domain databases

CDDicd07765 KRAB_A-box, 1 hit
InterProiView protein in InterPro
IPR001909 KRAB
IPR036051 KRAB_dom_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF01352 KRAB, 1 hit
SMARTiView protein in SMART
SM00349 KRAB, 1 hit
SM00355 ZnF_C2H2, 4 hits
SUPFAMiSSF109640 SSF109640, 1 hit
SSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS50805 KRAB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 3 hits
PS50157 ZINC_FINGER_C2H2_2, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C6P8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARKQSSQP SRTPVSYEDV AVSFTQEEWE YLTSTQKTLY QKVMSETFKN
60 70 80 90 100
LTFVGSKKKP QEPSSDLQDK NEEQEKSSSC TGVFKGGPFF FCLTCGKCFK
110 120 130 140 150
KNTFLFNHQF PVRSRRLAVT NPQSRKGKGY KAQHRGERPF FCNFCGKTYR
160 170 180 190 200
DASGLSRHRR AHLGYRPRSC PECGKCFRDQ SEVNRHLKVH QNKPAASNQA
210 220 230 240 250
GNQASNQRLK SRVPPTTPRS QAPALKYVKV IQGPVARAKA RNSGASTLNV
260 270 280 290 300
RSNSITVVRS REKISCPYCH ITFTMRTCLL THLKIHFRRQ PNQHFCCKES
310 320 330 340 350
AHSSNTLRMQ KIYTCPVCDS SFRGKESLLD HLCCQRPIRF SKCWEILGHL
360 370 380 390 400
LGYLHEPVVL GNIFKVRDSS GKRMESRRRR RKRACTENPE TEGLSGKGRV
410 420
APWEMEGATS PESPVTEEDS D
Length:421
Mass (Da):47,959
Last modified:March 1, 2003 - v1
Checksum:iC341340FFB5B490A
GO
Isoform 2 (identifier: Q8C6P8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     7-9: Missing.

Note: No experimental confirmation available.
Show »
Length:418
Mass (Da):47,656
Checksum:i17F1D82670BF31D2
GO

Sequence cautioni

The sequence AAR04560 differs from that shown. Numerous sequencing errors.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75E → K in BAA04876 (PubMed:8120052).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0263317 – 9Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21850 mRNA Translation: BAA04876.1
AY344233 mRNA Translation: AAR04560.1 Sequence problems.
AK054083 mRNA Translation: BAC35649.1
AK136241 mRNA Translation: BAE22892.1
AK139625 mRNA Translation: BAE24086.1
AK145368 mRNA Translation: BAE26393.1
BC052028 mRNA Translation: AAH52028.1
CCDSiCCDS28733.1 [Q8C6P8-1]
CCDS50106.1 [Q8C6P8-2]
PIRiA54375
RefSeqiNP_001013767.1, NM_001013745.2 [Q8C6P8-1]
NP_001161973.1, NM_001168501.1 [Q8C6P8-1]
NP_001161974.1, NM_001168502.1 [Q8C6P8-2]
XP_006524287.1, XM_006524224.1 [Q8C6P8-1]
XP_017172947.1, XM_017317458.1 [Q8C6P8-2]
XP_017172948.1, XM_017317459.1 [Q8C6P8-2]
UniGeneiMm.305561

Genome annotation databases

EnsembliENSMUST00000069250; ENSMUSP00000065811; ENSMUSG00000036036 [Q8C6P8-1]
ENSMUST00000089968; ENSMUSP00000087414; ENSMUSG00000036036 [Q8C6P8-2]
ENSMUST00000174524; ENSMUSP00000134418; ENSMUSG00000036036 [Q8C6P8-1]
ENSMUST00000174672; ENSMUSP00000133821; ENSMUSG00000036036 [Q8C6P8-1]
GeneIDi22715
KEGGimmu:22715
UCSCiuc008clu.2 mouse [Q8C6P8-2]
uc008clv.2 mouse [Q8C6P8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZFP57_MOUSE
AccessioniPrimary (citable) accession number: Q8C6P8
Secondary accession number(s): Q3UT94, Q62515, Q6JPI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health