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Protein

Zinc finger protein 57

Gene

Zfp57

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions. Required for the establishment of maternal methylation imprints at SNRPN locus. Acts as a transcriptional repressor in Schwann cells. Binds to a 5'-TGCCGC-3' consensus sequence and recognizes the methylated CpG within this element.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei178 – 1781Crucial for 5-methylcytosine recognition

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri90 – 11324C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri140 – 16223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri168 – 19023C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri264 – 28623C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri313 – 33220C2H2-type 5; degeneratePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA methylation involved in embryo development Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of gene expression by genetic imprinting Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 57
Short name:
Zfp-57
Gene namesi
Name:Zfp57
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:99204. Zfp57.

Subcellular locationi

GO - Cellular componenti

  • nuclear heterochromatin Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Affects the maintenance of DNA methylation imprints. The absence of just the zygotic function causes partial neonatal lethality, whereas eliminating both the maternal and zygotic functions results in a highly penetrant embryonic lethality. In oocytes, its absence results in failure to establish maternal methylation imprints at the Snrpn imprinted region. Intriguingly, methylation imprints are reacquired specifically at the maternally derived Snrpn imprinted region when the zygotic Zfp57 is present in embryos.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Zinc finger protein 57PRO_0000291965Add
BLAST

Proteomic databases

MaxQBiQ8C6P8.
PaxDbiQ8C6P8.
PeptideAtlasiQ8C6P8.
PRIDEiQ8C6P8.

PTM databases

iPTMnetiQ8C6P8.
PhosphoSiteiQ8C6P8.

Expressioni

Tissue specificityi

Expressed in oocytes and in a subset of adult tissues. Expressed at high levels in testis, and at low levels in cerebellum. Present in sciatic nerve and spinal cord (at protein level).2 Publications

Developmental stagei

Expression peaks between E11 and E17, and decreases from P0 to P28. Expressed in lung throughout embryonic development. At E12, expressed in spinal cord, dorsal root ganglia and sciatic nerve. At E15, highly expressed in all neural tissues. At P0, expressed in brain and spinal cord. Present in Schwann cells at E16 and P0 (at protein level). Maternal product is present in preimplantation embryos. Expressed in pluripotent embryonic stem cells. Down-regulated when embryonic stem cells differentiate.4 Publications

Inductioni

Up-regulated by LIF. Down-regulated during differentiation of embryonic stem cells, or during retinoic acid-induced differentiation of F9 cells (at protein level). Regulated by JMJD1A, which mediates histone H3K9Me2 demethylation at its promoter, thereby activating expression.5 Publications

Gene expression databases

BgeeiENSMUSG00000036036.
CleanExiMM_ZFP57.
ExpressionAtlasiQ8C6P8. baseline and differential.
GenevisibleiQ8C6P8. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065811.

Structurei

Secondary structure

1
421
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni143 – 1453Combined sources
Beta strandi148 – 1514Combined sources
Helixi152 – 16312Combined sources
Turni171 – 1733Combined sources
Beta strandi176 – 1794Combined sources
Helixi180 – 1867Combined sources
Helixi187 – 1904Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GZNX-ray0.99C137-195[»]
4M9VX-ray0.97C/F137-195[»]
ProteinModelPortaliQ8C6P8.
SMRiQ8C6P8. Positions 15-55, 90-289, 303-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 8874KRABPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi377 – 3837Poly-Arg

Domaini

The KRAB domain is required for function as transcriptional repressor.
Zinc fingers 2 and 3 mediate recognition of the target element, ZF2 interacting with the 5' half (TGC) and ZF3 interacting with the 3' half (CGC).

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri90 – 11324C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri140 – 16223C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri168 – 19023C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri264 – 28623C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri313 – 33220C2H2-type 5; degeneratePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00390000002599.
HOGENOMiHOG000155778.
HOVERGENiHBG108764.
InParanoidiQ8C6P8.
KOiK09228.
OMAiEEWEYLT.
OrthoDBiEOG091G0P6J.
PhylomeDBiQ8C6P8.
TreeFamiTF337947.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 2 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C6P8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARKQSSQP SRTPVSYEDV AVSFTQEEWE YLTSTQKTLY QKVMSETFKN
60 70 80 90 100
LTFVGSKKKP QEPSSDLQDK NEEQEKSSSC TGVFKGGPFF FCLTCGKCFK
110 120 130 140 150
KNTFLFNHQF PVRSRRLAVT NPQSRKGKGY KAQHRGERPF FCNFCGKTYR
160 170 180 190 200
DASGLSRHRR AHLGYRPRSC PECGKCFRDQ SEVNRHLKVH QNKPAASNQA
210 220 230 240 250
GNQASNQRLK SRVPPTTPRS QAPALKYVKV IQGPVARAKA RNSGASTLNV
260 270 280 290 300
RSNSITVVRS REKISCPYCH ITFTMRTCLL THLKIHFRRQ PNQHFCCKES
310 320 330 340 350
AHSSNTLRMQ KIYTCPVCDS SFRGKESLLD HLCCQRPIRF SKCWEILGHL
360 370 380 390 400
LGYLHEPVVL GNIFKVRDSS GKRMESRRRR RKRACTENPE TEGLSGKGRV
410 420
APWEMEGATS PESPVTEEDS D
Length:421
Mass (Da):47,959
Last modified:March 1, 2003 - v1
Checksum:iC341340FFB5B490A
GO
Isoform 2 (identifier: Q8C6P8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     7-9: Missing.

Note: No experimental confirmation available.
Show »
Length:418
Mass (Da):47,656
Checksum:i17F1D82670BF31D2
GO

Sequence cautioni

The sequence AAR04560 differs from that shown.Numerous sequencing errors.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751E → K in BAA04876 (PubMed:8120052).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei7 – 93Missing in isoform 2. 1 PublicationVSP_026331

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21850 mRNA. Translation: BAA04876.1.
AY344233 mRNA. Translation: AAR04560.1. Sequence problems.
AK054083 mRNA. Translation: BAC35649.1.
AK136241 mRNA. Translation: BAE22892.1.
AK139625 mRNA. Translation: BAE24086.1.
AK145368 mRNA. Translation: BAE26393.1.
BC052028 mRNA. Translation: AAH52028.1.
CCDSiCCDS28733.1. [Q8C6P8-1]
CCDS50106.1. [Q8C6P8-2]
PIRiA54375.
RefSeqiNP_001013767.1. NM_001013745.2. [Q8C6P8-1]
NP_001161973.1. NM_001168501.1. [Q8C6P8-1]
NP_001161974.1. NM_001168502.1. [Q8C6P8-2]
XP_006524286.1. XM_006524223.1. [Q8C6P8-1]
XP_006524287.1. XM_006524224.1. [Q8C6P8-1]
XP_006536661.1. XM_006536598.2. [Q8C6P8-1]
XP_006536900.1. XM_006536837.1. [Q8C6P8-1]
XP_006536901.1. XM_006536838.1. [Q8C6P8-1]
XP_006536902.1. XM_006536839.1. [Q8C6P8-1]
XP_006536904.1. XM_006536841.1. [Q8C6P8-2]
XP_006537393.1. XM_006537330.2. [Q8C6P8-1]
XP_006537394.1. XM_006537331.2. [Q8C6P8-1]
XP_006537395.1. XM_006537332.2. [Q8C6P8-2]
XP_006537396.1. XM_006537333.2. [Q8C6P8-2]
XP_011238178.1. XM_011239876.1. [Q8C6P8-1]
XP_011249803.1. XM_011251501.1. [Q8C6P8-2]
UniGeneiMm.305561.

Genome annotation databases

EnsembliENSMUST00000069250; ENSMUSP00000065811; ENSMUSG00000036036. [Q8C6P8-1]
ENSMUST00000089968; ENSMUSP00000087414; ENSMUSG00000036036. [Q8C6P8-2]
ENSMUST00000174524; ENSMUSP00000134418; ENSMUSG00000036036. [Q8C6P8-1]
ENSMUST00000174672; ENSMUSP00000133821; ENSMUSG00000036036. [Q8C6P8-1]
GeneIDi22715.
KEGGimmu:22715.
UCSCiuc008clu.2. mouse. [Q8C6P8-2]
uc008clv.2. mouse. [Q8C6P8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21850 mRNA. Translation: BAA04876.1.
AY344233 mRNA. Translation: AAR04560.1. Sequence problems.
AK054083 mRNA. Translation: BAC35649.1.
AK136241 mRNA. Translation: BAE22892.1.
AK139625 mRNA. Translation: BAE24086.1.
AK145368 mRNA. Translation: BAE26393.1.
BC052028 mRNA. Translation: AAH52028.1.
CCDSiCCDS28733.1. [Q8C6P8-1]
CCDS50106.1. [Q8C6P8-2]
PIRiA54375.
RefSeqiNP_001013767.1. NM_001013745.2. [Q8C6P8-1]
NP_001161973.1. NM_001168501.1. [Q8C6P8-1]
NP_001161974.1. NM_001168502.1. [Q8C6P8-2]
XP_006524286.1. XM_006524223.1. [Q8C6P8-1]
XP_006524287.1. XM_006524224.1. [Q8C6P8-1]
XP_006536661.1. XM_006536598.2. [Q8C6P8-1]
XP_006536900.1. XM_006536837.1. [Q8C6P8-1]
XP_006536901.1. XM_006536838.1. [Q8C6P8-1]
XP_006536902.1. XM_006536839.1. [Q8C6P8-1]
XP_006536904.1. XM_006536841.1. [Q8C6P8-2]
XP_006537393.1. XM_006537330.2. [Q8C6P8-1]
XP_006537394.1. XM_006537331.2. [Q8C6P8-1]
XP_006537395.1. XM_006537332.2. [Q8C6P8-2]
XP_006537396.1. XM_006537333.2. [Q8C6P8-2]
XP_011238178.1. XM_011239876.1. [Q8C6P8-1]
XP_011249803.1. XM_011251501.1. [Q8C6P8-2]
UniGeneiMm.305561.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GZNX-ray0.99C137-195[»]
4M9VX-ray0.97C/F137-195[»]
ProteinModelPortaliQ8C6P8.
SMRiQ8C6P8. Positions 15-55, 90-289, 303-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065811.

PTM databases

iPTMnetiQ8C6P8.
PhosphoSiteiQ8C6P8.

Proteomic databases

MaxQBiQ8C6P8.
PaxDbiQ8C6P8.
PeptideAtlasiQ8C6P8.
PRIDEiQ8C6P8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069250; ENSMUSP00000065811; ENSMUSG00000036036. [Q8C6P8-1]
ENSMUST00000089968; ENSMUSP00000087414; ENSMUSG00000036036. [Q8C6P8-2]
ENSMUST00000174524; ENSMUSP00000134418; ENSMUSG00000036036. [Q8C6P8-1]
ENSMUST00000174672; ENSMUSP00000133821; ENSMUSG00000036036. [Q8C6P8-1]
GeneIDi22715.
KEGGimmu:22715.
UCSCiuc008clu.2. mouse. [Q8C6P8-2]
uc008clv.2. mouse. [Q8C6P8-1]

Organism-specific databases

CTDi346171.
MGIiMGI:99204. Zfp57.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00390000002599.
HOGENOMiHOG000155778.
HOVERGENiHBG108764.
InParanoidiQ8C6P8.
KOiK09228.
OMAiEEWEYLT.
OrthoDBiEOG091G0P6J.
PhylomeDBiQ8C6P8.
TreeFamiTF337947.

Miscellaneous databases

ChiTaRSiZfp57. mouse.
PROiQ8C6P8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036036.
CleanExiMM_ZFP57.
ExpressionAtlasiQ8C6P8. baseline and differential.
GenevisibleiQ8C6P8. MM.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 2 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZFP57_MOUSE
AccessioniPrimary (citable) accession number: Q8C6P8
Secondary accession number(s): Q3UT94, Q62515, Q6JPI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.