Reviewed, UniProtKB/Swiss-Prot Q8C6I2 (SDHF2_MOUSE)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase assembly factor 2, mitochondrial
      Short name=SDH assembly factor 2
Alternative name(s):
    Succinate dehydrogenase subunit 5, mitochondrial

Gene names

Name:	Sdhaf2
Synonyms:	Pgl2, Sdh5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for insertion of FAD cofactor into SDHA, the catalytic subunit of succinate dehydrogenase (SDH). SDH is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). In is unclear whether it participates in the chemistry of FAD attachment (enzymatic function) or acts as a chaperone that maintains SDHA in a conformation that is susceptible to autocatalytic FAD attachment By similarity.
Subunit structure	Interacts with SDHA By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the SDHAF2 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Gene Ontology (GO)
Biological process	mitochondrial electron transport, succinate to ubiquinone Inferred from sequence or structural similarity. Source: UniProtKB protein-FAD linkage Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8C6I2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8C6I2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-164: EAKPAPEIFENEVMELLREFAKNKNKEQRLRAPDLEYLFEKPH → GMPYAELHLGIIR

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8C6I2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion Potential
	Chain	28 – 164	137	Succinate dehydrogenase assembly factor 2, mitochondrial		PRO_0000294358
Natural variations
	Alternative sequence	1 – 94	94	Missing in isoform 3.		VSP_026633
	Alternative sequence	122 – 164	43	EAKPA…FEKPH → GMPYAELHLGIIR in isoform 2.		VSP_026634
Experimental info
	Sequence conflict	27	1	T → K in BAB28863. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CDC12D451D532BA4	FASTA	164	19,431
	10 20 30 40 50 60 MAVVTLIPTL ARALSKHSLL SPLPSVTSFR RFYRGDSPTD SQKDMIEIPL PPWQERTDES 70 80 90 100 110 120 IETKRARLLY ESRKRGMLEN CILLSLFAKE YLHNMTEKQL NLYDRLINEP SNDWDIYYWA 130 140 150 160 TEAKPAPEIF ENEVMELLRE FAKNKNKEQR LRAPDLEYLF EKPH
	Isoform 2. Checksum: 47578142A53EB5C0	FASTA	134	15,702
	10 20 30 40 50 60 MAVVTLIPTL ARALSKHSLL SPLPSVTSFR RFYRGDSPTD SQKDMIEIPL PPWQERTDES 70 80 90 100 110 120 IETKRARLLY ESRKRGMLEN CILLSLFAKE YLHNMTEKQL NLYDRLINEP SNDWDIYYWA 130 TGMPYAELHL GIIR
	Isoform 3. Checksum: 11F13375979B4CD9	FASTA	70	8,574
	10 20 30 40 50 60 MTEKQLNLYD RLINEPSNDW DIYYWATEAK PAPEIFENEV MELLREFAKN KNKEQRLRAP 70 DLEYLFEKPH

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Head, Kidney and Stomach.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6 and FVB/N. Tissue: Brain, Embryo, Eye and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK160175 mRNA. Translation: BAE35674.1. AK013454 mRNA. Translation: BAB28863.1. AK075594 mRNA. Translation: BAC35843.1. AK160957 mRNA. Translation: BAE36113.1. AK167228 mRNA. Translation: BAE39352.1. BC029630 mRNA. Translation: AAH29630.1. BC038924 mRNA. Translation: AAH38924.1. BC048913 mRNA. Translation: AAH48913.1. BC060090 mRNA. Translation: AAH60090.1. BC085277 mRNA. Translation: AAH85277.1.
IPI	IPI00312113. IPI00461231. IPI00853857.
RefSeq	NP_079609.2.
UniGene	Mm.180063 Mm.293980
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8C6I2.
Proteomic databases
PRIDE	Q8C6I2.
Genome annotation databases
Ensembl	ENSMUST00000025570; ENSMUSP00000025570; ENSMUSG00000024668; Mus musculus. [Genome view]
GeneID	66072.
KEGG	mmu:66072.
NMPDR	fig|10090.3.peg.4662.
UCSC	uc008gpt.1. mouse.
Organism-specific databases
CTD	66072.
MGI	MGI:1913322. Sdhaf2.
Phylogenomic databases
HOGENOM	Q8C6I2.
HOVERGEN	Q8C6I2.
OMA	IFENEVM
OrthoDB	EOG9P5NWK
Gene expression databases
ArrayExpress	Q8C6I2.
Bgee	Q8C6I2.
CleanEx	MM_0610038F07RIK.
Genevestigator	Q8C6I2.
Family and domain databases
InterPro	IPR005631. DUF339. [Graphical view]
Gene3D	G3DSA:1.10.150.250. DUF339. 1 hit.
Pfam	PF03937. DUF339. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	320544.
SOURCE	Search...

Entry information

Entry name

SDHF2_MOUSE

Accession

Primary (citable) accession number: Q8C6I2
Secondary accession number(s): Q3TVE5 , Q6PAS5, Q80ZJ9, Q8K2W8, Q9CYQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)