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Q8C6B2 (RTKN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rhotekin
Gene names
Name:Rtkn
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures By similarity.

Subunit structure

Interacts via its C-terminal region with the TAX1BP3 PDZ domain. This interaction facilitates Rho-mediated activation of the c-Fos serum response element (SRE). Interacts with SEPT9 By similarity. Specifically binds to GTP-bound RHOA, RHOB and RHOC and inhibits their GTPase activity. Ref.1 UniProtKB Q9BST9

Tissue specificity

Abundantly expressed in brain and kidney. Weakly expressed in lung, testis, skeletal muscle, heart and thymus. Ref.1

Sequence similarities

Contains 1 PH domain.

Contains 1 REM (Hr1) repeat.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.2 (identifier: Q8C6B2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 2 Ref.1 (identifier: Q8C6B2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MFSRNHRSRITVARGSALEMEFKRGRFRLSFFSESP → MQDRLRILEDLNMLYIRQMALSL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564Rhotekin
PRO_0000233941

Regions

Repeat36 – 9964REM
Domain309 – 416108PH
Compositional bias479 – 54567Pro-rich

Amino acid modifications

Modified residue1061Phosphoserine Ref.5
Modified residue2321Phosphoserine By similarity
Modified residue5201Phosphoserine Ref.5
Modified residue5441Phosphoserine By similarity

Natural variations

Alternative sequence1 – 3636MFSRN…FSESP → MQDRLRILEDLNMLYIRQMA LSL in isoform 2. Ref.1
VSP_052006

Experimental info

Sequence conflict671Q → L in BAC36222. Ref.2
Sequence conflict3651R → G in AAH13820. Ref.4
Sequence conflict4441A → V in AAC52605. Ref.1
Sequence conflict4441A → V in AAH13820. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 0934EEBF09B12A3E

FASTA56463,013
        10         20         30         40         50         60 
MFSRNHRSRI TVARGSALEM EFKRGRFRLS FFSESPEDTE LQRKLDHEIR MRDGACKLLA 

        70         80         90        100        110        120 
ACSQREQALE ATKSLLVCNS RILSYMGELQ RRKEAQVLEK TGRRPSDSVQ PAQHSPCRGR 

       130        140        150        160        170        180 
VCISDLRIPL MWKDTEYFKN KGDLHRWAVF LLLQIGEQIQ DTEMVLVDRT LTDISFQNNV 

       190        200        210        220        230        240 
LFAEAEPDFE LRLELYGACV EEEGALAGAP KRLATKLSSS LGRSSGKRVR ASLDSAGASG 

       250        260        270        280        290        300 
NSPVLLPTPA VGGPRFHLLA HTTLTLEEVQ DGFRTHDLTL TSHEENPAWL PLYGSVCCRL 

       310        320        330        340        350        360 
AAQPLCMIQP TASGALRVQQ AGELQNGTLV HGVLKGTNLF CYWRSEDADT GQEPLFTIVI 

       370        380        390        400        410        420 
NKETRVRAGE LEQAPEWPFT LSISNKYGDD EVTNTLQLES REALQNWMEA LWQLFFDMSQ 

       430        440        450        460        470        480 
WRHCCDEVMK IETPAPRKPP QALAKQGSLY HEMAIEPLDD IAAVTDILAQ REGTRLEPSP 

       490        500        510        520        530        540 
PWLAMFTDQP ALPSSCSPAS VAPVPTWMQP LPWGRPRTFS LDAAPADHSL GPSRSVAPLP 

       550        560 
PQRSPKSRGF YSKSQLGPWL QSPV

« Hide

Isoform 2 [UniParc].

Checksum: EA3D925FFF0FB85B
Show »

FASTA55161,557

References

« Hide 'large scale' references
[1]"Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain."
Reid T., Furuyashiki T., Ishizaki T., Watanabe G., Watanabe N., Fujisawa K., Morii N., Madaule P., Narumiya S.
J. Biol. Chem. 271:13556-13560(1996) [PubMed: 8662891] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH RHOA; RHOB AND RHOC, TISSUE SPECIFICITY.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Head.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: FVB/N.
Tissue: Mammary gland.
[5]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-520, MASS SPECTROMETRY.
Tissue: Melanoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U54638 mRNA. Translation: AAC52605.1.
AK076155 mRNA. Translation: BAC36222.1.
AC104324 Genomic DNA. No translation available.
BC013820 mRNA. Translation: AAH13820.1.
IPIIPI00226220.
IPI01008657.
RefSeqNP_001129699.1. NM_001136227.1.
NP_033132.2. NM_009106.2.
NP_598396.2. NM_133641.2.
UniGeneMm.4139.

3D structure databases

ProteinModelPortalQ8C6B2.
SMRQ8C6B2. Positions 310-415.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29983N.
IntActQ8C6B2. 4 interactions.
MINTMINT-1791510.
STRINGQ8C6B2.

PTM databases

PhosphoSiteQ8C6B2.

Proteomic databases

PRIDEQ8C6B2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000065512; ENSMUSP00000065571; ENSMUSG00000034930.
ENSMUST00000087938; ENSMUSP00000085249; ENSMUSG00000034930.
ENSMUST00000121093; ENSMUSP00000112501; ENSMUSG00000034930.
GeneID20166.
KEGGmmu:20166.
UCSCuc009cms.2. mouse.
uc009cmt.2. mouse.

Organism-specific databases

CTD6242.
MGIMGI:107371. Rtkn.

Phylogenomic databases

eggNOGroNOG11996.
GeneTreeENSGT00390000000104.
HOGENOMHBG715547.
HOVERGENHBG059480.
InParanoidQ8C6B2.
OrthoDBEOG46143R.

Gene expression databases

ArrayExpressQ8C6B2.
BgeeQ8C6B2.
CleanExMM_RTKN.
GenevestigatorQ8C6B2.
GermOnlineENSMUSG00000034930. Mus musculus.

Family and domain databases

InterProIPR011072. HR1_rho-bd.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 1 hit.
PfamPF02185. HR1. 1 hit.
[Graphical view]
SMARTSM00742. Hr1. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
PROSITEPS50003. PH_DOMAIN. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio297673.
SOURCESearch...

Entry information

Entry nameRTKN_MOUSE
AccessionPrimary (citable) accession number: Q8C6B2
Secondary accession number(s): E9QM24, Q61192, Q8VIG7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 27, 2011
Last modified: January 25, 2012
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents