Reviewed, UniProtKB/Swiss-Prot Q8C6B2 (RTKN_MOUSE)

Names and origin

Protein names

Recommended name:
    Rhotekin

Gene names

Name:

Rtkn

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures By similarity.
Subunit structure	Interacts via its C-terminal region with the TAX1BP3 PDZ domain. This interaction facilitates Rho-mediated activation of the c-Fos serum response element (SRE). Interacts with SEPT9 By similarity. Specifically binds to GTP-bound RHOA, RHOB and RHOC and inhibits their GTPase activity. Ref.1 UniProtKB Q9BST9
Tissue specificity	Abundantly expressed in brain and kidney. Weakly expressed in lung, testis, skeletal muscle, heart and thymus. Ref.1
Sequence similarities	Contains 1 PH domain. Contains 1 REM (Hr1) repeat.

Ontologies

Keywords
Biological process	Apoptosis
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	Rho protein signal transduction Inferred from sequence or structural similarity. Source: UniProtKB apoptosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP-Rho binding Ref.1 Inferred from direct assay. Source: UniProtKB GTPase inhibitor activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rho1	Q09914	1	EBI-1162461,EBI-1559286	From a different organism.
Tax1bp3	Q9DBG9	1	EBI-1162441,EBI-1161647

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.2 (identifier: Q8C6B2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 Ref.1 (identifier: Q8C6B2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MFSRNHRSRITVARGSALEMEFKRGRFRLSFFSESP → MQDRLRILEDLNMLYIRQMALSL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Rhotekin		PRO_0000233941
Regions
	Repeat	36 – 99	64	REM
	Domain	309 – 416	108	PH
	Compositional bias	479 – 545	67	Pro-rich
Amino acid modifications
	Modified residue	106	1	Phosphoserine Ref.4
	Modified residue	232	1	Phosphoserine By similarity
	Modified residue	520	1	Phosphoserine Ref.4
	Modified residue	544	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 36	36	MFSRN…FSESP → MQDRLRILEDLNMLYIRQMA LSL in isoform 2. Ref.1		VSP_052006
Experimental info
	Sequence conflict	67	1	Q → L in BAC36222. Ref.2
	Sequence conflict	365	1	R → G Ref.1
	Sequence conflict	365	1	R → G Ref.2
	Sequence conflict	444	1	V → A in BAC36222. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: 0124F9BF09B13D3E	FASTA	564	63,041
	10 20 30 40 50 60 MFSRNHRSRI TVARGSALEM EFKRGRFRLS FFSESPEDTE LQRKLDHEIR MRDGACKLLA 70 80 90 100 110 120 ACSQREQALE ATKSLLVCNS RILSYMGELQ RRKEAQVLEK TGRRPSDSVQ PAQHSPCRGR 130 140 150 160 170 180 VCISDLRIPL MWKDTEYFKN KGDLHRWAVF LLLQIGEQIQ DTEMVLVDRT LTDISFQNNV 190 200 210 220 230 240 LFAEAEPDFE LRLELYGACV EEEGALAGAP KRLATKLSSS LGRSSGKRVR ASLDSAGASG 250 260 270 280 290 300 NSPVLLPTPA VGGPRFHLLA HTTLTLEEVQ DGFRTHDLTL TSHEENPAWL PLYGSVCCRL 310 320 330 340 350 360 AAQPLCMIQP TASGALRVQQ AGELQNGTLV HGVLKGTNLF CYWRSEDADT GQEPLFTIVI 370 380 390 400 410 420 NKETRVRAGE LEQAPEWPFT LSISNKYGDD EVTNTLQLES REALQNWMEA LWQLFFDMSQ 430 440 450 460 470 480 WRHCCDEVMK IETPAPRKPP QALVKQGSLY HEMAIEPLDD IAAVTDILAQ REGTRLEPSP 490 500 510 520 530 540 PWLAMFTDQP ALPSSCSPAS VAPVPTWMQP LPWGRPRTFS LDAAPADHSL GPSRSVAPLP 550 560 PQRSPKSRGF YSKSQLGPWL QSPV
	Isoform 2. Checksum: E22D855FFF0FAF5B	FASTA	551	61,585
	10 20 30 40 50 60 MQDRLRILED LNMLYIRQMA LSLEDTELQR KLDHEIRMRD GACKLLAACS QREQALEATK 70 80 90 100 110 120 SLLVCNSRIL SYMGELQRRK EAQVLEKTGR RPSDSVQPAQ HSPCRGRVCI SDLRIPLMWK 130 140 150 160 170 180 DTEYFKNKGD LHRWAVFLLL QIGEQIQDTE MVLVDRTLTD ISFQNNVLFA EAEPDFELRL 190 200 210 220 230 240 ELYGACVEEE GALAGAPKRL ATKLSSSLGR SSGKRVRASL DSAGASGNSP VLLPTPAVGG 250 260 270 280 290 300 PRFHLLAHTT LTLEEVQDGF RTHDLTLTSH EENPAWLPLY GSVCCRLAAQ PLCMIQPTAS 310 320 330 340 350 360 GALRVQQAGE LQNGTLVHGV LKGTNLFCYW RSEDADTGQE PLFTIVINKE TRVRAGELEQ 370 380 390 400 410 420 APEWPFTLSI SNKYGDDEVT NTLQLESREA LQNWMEALWQ LFFDMSQWRH CCDEVMKIET 430 440 450 460 470 480 PAPRKPPQAL VKQGSLYHEM AIEPLDDIAA VTDILAQREG TRLEPSPPWL AMFTDQPALP 490 500 510 520 530 540 SSCSPASVAP VPTWMQPLPW GRPRTFSLDA APADHSLGPS RSVAPLPPQR SPKSRGFYSK 550 SQLGPWLQSP V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain." Reid T., Furuyashiki T., Ishizaki T., Watanabe G., Watanabe N., Fujisawa K., Morii N., Madaule P., Narumiya S. J. Biol. Chem. 271:13556-13560(1996) [PubMed: 8662891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH RHOA; RHOB AND RHOC, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Head.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: FVB/N. Tissue: Mammary gland.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-106 AND SER-520, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U54638 mRNA. Translation: AAC52605.1. AK076155 mRNA. Translation: BAC36222.1. BC013820 mRNA. Translation: AAH13820.1.
IPI	IPI00226220. IPI00278604.
RefSeq	NP_001129699.1. NP_033132.2. NP_598396.2.
UniGene	Mm.4139
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8C6B2. 2 interactions.
STRING	Q8C6B2.
PTM databases
PhosphoSite	Q8C6B2.
Proteomic databases
PRIDE	Q8C6B2.
Genome annotation databases
Ensembl	ENSMUST00000065512; ENSMUSP00000065571; ENSMUSG00000034930; Mus musculus. [Genome view] ENSMUST00000087938; ENSMUSP00000085249; ENSMUSG00000034930; Mus musculus. [Genome view]
GeneID	20166.
KEGG	mmu:20166.
UCSC	uc009cms.1. mouse. uc009cmt.1. mouse.
Organism-specific databases
CTD	20166.
MGI	MGI:107371. Rtkn.
Phylogenomic databases
HOGENOM	Q8C6B2.
HOVERGEN	Q8C6B2.
Gene expression databases
ArrayExpress	Q8C6B2.
Bgee	Q8C6B2.
CleanEx	MM_RTKN.
Genevestigator	Q8C6B2.
GermOnline	ENSMUSG00000034930. Mus musculus.
Family and domain databases
InterPro	IPR011072. HR1_rho-bd. IPR001849. Pleckstrin_homology. [Graphical view]
SMART	SM00742. Hr1. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	297673.
SOURCE	Search...

Entry information

Entry name

RTKN_MOUSE

Accession

Primary (citable) accession number: Q8C6B2
Secondary accession number(s): Q61192, Q8VIG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	October 13, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)