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Protein

E3 ubiquitin-protein ligase pellino homolog 1

Gene

Peli1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: MGI

GO - Biological processi

  • negative regulation of NF-kappaB transcription factor activity Source: MGI
  • negative regulation of T cell activation Source: MGI
  • negative regulation of T cell proliferation Source: MGI
  • positive regulation of B cell activation Source: MGI
  • positive regulation of B cell proliferation Source: MGI
  • positive regulation of cytokine production Source: MGI
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
  • positive regulation of protein ubiquitination Source: MGI
  • positive regulation of toll-like receptor 3 signaling pathway Source: MGI
  • positive regulation of toll-like receptor 4 signaling pathway Source: MGI
  • protein K48-linked ubiquitination Source: MGI
  • protein polyubiquitination Source: MGI
  • response to dsRNA Source: MGI
  • response to lipopolysaccharide Source: MGI
  • Toll signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-446652. Interleukin-1 signaling.
R-MMU-937039. IRAK1 recruits IKK complex.
R-MMU-975144. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase pellino homolog 1 (EC:6.3.2.-)
Short name:
Pellino-1
Gene namesi
Name:Peli1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914495. Peli1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941731 – 418E3 ubiquitin-protein ligase pellino homolog 1Add BLAST418

Post-translational modificationi

Phosphorylated by IRAK1 and IRAK4 enhancing its E3 ligase activity.By similarity
Sumoylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8C669.
PaxDbiQ8C669.
PRIDEiQ8C669.

PTM databases

iPTMnetiQ8C669.
PhosphoSitePlusiQ8C669.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020134.
CleanExiMM_PELI1.
ExpressionAtlasiQ8C669. baseline and differential.
GenevisibleiQ8C669. MM.

Interactioni

Subunit structurei

Interacts with MAP3K7 (By similarity). Upon IL1B treatment, forms a complex with TRAF6, IRAK1, IRAK4 and MYD88; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Direct binding of SMAD6 to PELI1 prevents the complex formation and hence negatively regulates IL1R-TLR signaling and eventually NF-kappa-B-mediated gene expression.By similarity1 Publication

Protein-protein interaction databases

BioGridi212043. 3 interactors.
DIPiDIP-48336N.
IntActiQ8C669. 1 interactor.
STRINGi10090.ENSMUSP00000090979.

Structurei

3D structure databases

ProteinModelPortaliQ8C669.
SMRiQ8C669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pellino family.Curated

Phylogenomic databases

eggNOGiKOG3842. Eukaryota.
ENOG410XP3S. LUCA.
GeneTreeiENSGT00390000007680.
HOGENOMiHOG000234110.
HOVERGENiHBG053559.
InParanoidiQ8C669.
KOiK11964.
OMAiSGEQGYI.
OrthoDBiEOG091G0631.
PhylomeDBiQ8C669.
TreeFamiTF314338.

Family and domain databases

InterProiIPR006800. Pellino_fam.
[Graphical view]
PANTHERiPTHR12098. PTHR12098. 1 hit.
PfamiPF04710. Pellino. 1 hit.
[Graphical view]
PIRSFiPIRSF038886. Pellino. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8C669-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSPDQENHP SKAPVKYGEL IVLGYNGSLP NGDRGRRKSR FALFKRPKAN
60 70 80 90 100
GVKPSTVHIA CTPQAAKAIS NKDQHSISYT LSRAQTVVVE YTHDSNTDMF
110 120 130 140 150
QIGRSTESPI DFVVTDTVPG SQSNSDTQSV QSTISRFACR IICERSPPFT
160 170 180 190 200
ARIYAAGFDS SKNIFLGEKA AKWKTSDGQM DGLTTNGVLV MHPRNGFTED
210 220 230 240 250
SKPGIWREIS VCGNVFSLRE TRSAQQRGKM VEIETNQLQD GSLIDLCGAT
260 270 280 290 300
LLWRTAEGLS HTPTVKHLEA LRQEINAARP QCPVGFNTLA FPSMKRKDVV
310 320 330 340 350
DEKQPWVYLN CGHVHGYHNW GNKEERDGKD RECPMCRSVG PYVPLWLGCE
360 370 380 390 400
AGFYVDAGPP THAFSPCGHV CSEKTTAYWS QIPLPHGTHT FHAACPFCAH
410
QLAGEQGYIR LIFQGPLD
Length:418
Mass (Da):46,259
Last modified:October 24, 2003 - v2
Checksum:i26072654577EBBF7
GO

Sequence cautioni

The sequence BAC36351 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5 – 7DQE → GSR in AAG15391 (PubMed:11306823).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302503 mRNA. Translation: AAG15391.1.
AK009945 mRNA. Translation: BAB26600.1.
AK045673 mRNA. Translation: BAC32452.1.
AK076458 mRNA. Translation: BAC36351.1. Different initiation.
BC016515 mRNA. Translation: AAH16515.1.
CCDSiCCDS24462.1.
RefSeqiNP_075813.2. NM_023324.2.
UniGeneiMm.28957.

Genome annotation databases

EnsembliENSMUST00000093290; ENSMUSP00000090979; ENSMUSG00000020134.
ENSMUST00000101477; ENSMUSP00000099018; ENSMUSG00000020134.
GeneIDi67245.
KEGGimmu:67245.
UCSCiuc007idl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302503 mRNA. Translation: AAG15391.1.
AK009945 mRNA. Translation: BAB26600.1.
AK045673 mRNA. Translation: BAC32452.1.
AK076458 mRNA. Translation: BAC36351.1. Different initiation.
BC016515 mRNA. Translation: AAH16515.1.
CCDSiCCDS24462.1.
RefSeqiNP_075813.2. NM_023324.2.
UniGeneiMm.28957.

3D structure databases

ProteinModelPortaliQ8C669.
SMRiQ8C669.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212043. 3 interactors.
DIPiDIP-48336N.
IntActiQ8C669. 1 interactor.
STRINGi10090.ENSMUSP00000090979.

PTM databases

iPTMnetiQ8C669.
PhosphoSitePlusiQ8C669.

Proteomic databases

MaxQBiQ8C669.
PaxDbiQ8C669.
PRIDEiQ8C669.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093290; ENSMUSP00000090979; ENSMUSG00000020134.
ENSMUST00000101477; ENSMUSP00000099018; ENSMUSG00000020134.
GeneIDi67245.
KEGGimmu:67245.
UCSCiuc007idl.1. mouse.

Organism-specific databases

CTDi57162.
MGIiMGI:1914495. Peli1.

Phylogenomic databases

eggNOGiKOG3842. Eukaryota.
ENOG410XP3S. LUCA.
GeneTreeiENSGT00390000007680.
HOGENOMiHOG000234110.
HOVERGENiHBG053559.
InParanoidiQ8C669.
KOiK11964.
OMAiSGEQGYI.
OrthoDBiEOG091G0631.
PhylomeDBiQ8C669.
TreeFamiTF314338.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-446652. Interleukin-1 signaling.
R-MMU-937039. IRAK1 recruits IKK complex.
R-MMU-975144. IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation.

Miscellaneous databases

ChiTaRSiPeli1. mouse.
PROiQ8C669.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020134.
CleanExiMM_PELI1.
ExpressionAtlasiQ8C669. baseline and differential.
GenevisibleiQ8C669. MM.

Family and domain databases

InterProiIPR006800. Pellino_fam.
[Graphical view]
PANTHERiPTHR12098. PTHR12098. 1 hit.
PfamiPF04710. Pellino. 1 hit.
[Graphical view]
PIRSFiPIRSF038886. Pellino. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPELI1_MOUSE
AccessioniPrimary (citable) accession number: Q8C669
Secondary accession number(s): Q91YL9, Q9CV22, Q9ERJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: October 24, 2003
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.