Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Meiotic recombination protein REC8 homolog

Gene

Rec8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.4 Publications

GO - Molecular functioni

GO - Biological processi

  • double-strand break repair via homologous recombination Source: MGI
  • fertilization Source: MGI
  • male meiosis I Source: MGI
  • meiotic nuclear division Source: MGI
  • meiotic sister chromatid cohesion, centromeric Source: GO_Central
  • mitotic sister chromatid cohesion Source: GO_Central
  • oocyte maturation Source: MGI
  • seminiferous tubule development Source: MGI
  • spermatid development Source: MGI
  • synapsis Source: MGI
  • synaptonemal complex assembly Source: MGI
Complete GO annotation...

Keywords - Biological processi

Chromosome partition, Meiosis

Enzyme and pathway databases

ReactomeiR-MMU-1221632. Meiotic synapsis.
R-MMU-1221633. Meiotic Synapsis.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein REC8 homolog
Alternative name(s):
Cohesin Rec8p
Gene namesi
Name:Rec8
Synonyms:Mei8, Rec8L1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1929645. Rec8.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome Source: MGI
  • condensed nuclear chromosome, centromeric region Source: MGI
  • condensed nuclear chromosome kinetochore Source: MGI
  • lateral element Source: MGI
  • male germ cell nucleus Source: MGI
  • meiotic cohesin complex Source: MGI
  • nuclear meiotic cohesin complex Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
  • synaptonemal complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display a high mortality rate, both during embryogenesis and after birth, germ cell failure and sterility. Mutant females exhibit ovarian dysgenesis and lack ovarian follicles at reproductive maturity. Affected males have small testes due to arrest of spermatogenesis during meiotic prophase I. Early chromosome pairing appears normal but synapsis occurs between sister chromatids rather than between homologous chromosomes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000978791 – 591Meiotic recombination protein REC8 homologAdd BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei149PhosphoserineCombined sources1
Modified residuei164PhosphothreonineBy similarity1
Modified residuei192PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8C5S7.
PeptideAtlasiQ8C5S7.
PRIDEiQ8C5S7.

PTM databases

iPTMnetiQ8C5S7.
PhosphoSitePlusiQ8C5S7.

Expressioni

Tissue specificityi

Expressed primarily in the gonads. In the testis, expressed in pachytene spermatocytes and in spermatids. Not expressed in spermatogonia or somatic cells. In the ovary, expressed only in oocytes. Low levels also detected in a number of somatic tissues including thymus, lung, liver, kidney and small intestine.4 Publications

Developmental stagei

Expressed from 2 weeks postpartum (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000002324.
CleanExiMM_REC8.
GenevisibleiQ8C5S7. MM.

Interactioni

Subunit structurei

Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51.1 Publication

Protein-protein interaction databases

BioGridi208153. 1 interactor.
DIPiDIP-60730N.
IntActiQ8C5S7. 4 interactors.
STRINGi10090.ENSMUSP00000002395.

Structurei

3D structure databases

ProteinModelPortaliQ8C5S7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the rad21 family.Curated

Phylogenomic databases

eggNOGiKOG1213. Eukaryota.
ENOG410XRB4. LUCA.
GeneTreeiENSGT00390000011379.
HOGENOMiHOG000154087.
HOVERGENiHBG056766.
InParanoidiQ8C5S7.
KOiK13054.
OMAiETQISRK.
OrthoDBiEOG091G0CXC.
PhylomeDBiQ8C5S7.
TreeFamiTF338144.

Family and domain databases

Gene3Di1.10.10.580. 1 hit.
InterProiIPR023093. Rad21/Rec8_C.
IPR006909. Rad21/Rec8_C_eu.
IPR006910. Rad21_Rec8_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04824. Rad21_Rec8. 1 hit.
PF04825. Rad21_Rec8_N. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8C5S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYYPNVLQR HTGCFATIWL AATRGSRLVK REYLNVNVVK TCEEILNYVL
60 70 80 90 100
VRVQPPVAGL PRPRFSLYLS AQLQIGVIRV YFQQCQYLVE DIQHILEHLH
110 120 130 140 150
RAQLRIRIDM EEADLPSLLL PNCLAMMETL EDAPEPFFGK MSVDPRLPSP
160 170 180 190 200
FDIPQIRHLL EAATPEKTRK ETLPEATPDP RKPDRTLATV QSPEVITLQE
210 220 230 240 250
AEPIRMLQIE GEQDLPEISR GDLELLIAEK DDAILLEERQ RGRLLRQRRA
260 270 280 290 300
SLPLDESREE PRALEGAGLV SALSPPAPAQ VEGIQEALPG QVFPPEVQKM
310 320 330 340 350
TGWEPGALLT EVTPPQELRL PAPPSTEKRL PSLQRPLPRR HRRRQLLFWD
360 370 380 390 400
KETQISREKF EEQLQTGAHC WEYPVAQPPK RMLTSPAELF RTPTLSGWLP
410 420 430 440 450
PELLGLWTHC AQVPQRMLRQ RPQLETEETV EEERAADEEE RRKTEALSEI
460 470 480 490 500
EVLREAQEPS GPLMLSSELS LEAAEDEKSR TSLIPPEWWA WSEEGQPEPP
510 520 530 540 550
ALPMLPELPE VPMEMPPRPE LSSEAVLRAV ALKLQANKEL DFSSLVPPLS
560 570 580 590
PRKLASRVFY LLLVLSTQKI LLVEQQKPYG PLLIRPGPKF P
Length:591
Mass (Da):67,425
Last modified:March 1, 2003 - v1
Checksum:i622986F564735700
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti49V → E in AAF69524 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262055 mRNA. Translation: AAF69524.1.
AK077167 mRNA. Translation: BAC36657.1.
AK146832 mRNA. Translation: BAE27467.1.
BC052155 mRNA. Translation: AAH52155.1.
CCDSiCCDS27120.1.
RefSeqiNP_064386.2. NM_020002.3.
UniGeneiMm.23149.

Genome annotation databases

EnsembliENSMUST00000002395; ENSMUSP00000002395; ENSMUSG00000002324.
GeneIDi56739.
KEGGimmu:56739.
UCSCiuc007tzo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262055 mRNA. Translation: AAF69524.1.
AK077167 mRNA. Translation: BAC36657.1.
AK146832 mRNA. Translation: BAE27467.1.
BC052155 mRNA. Translation: AAH52155.1.
CCDSiCCDS27120.1.
RefSeqiNP_064386.2. NM_020002.3.
UniGeneiMm.23149.

3D structure databases

ProteinModelPortaliQ8C5S7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208153. 1 interactor.
DIPiDIP-60730N.
IntActiQ8C5S7. 4 interactors.
STRINGi10090.ENSMUSP00000002395.

PTM databases

iPTMnetiQ8C5S7.
PhosphoSitePlusiQ8C5S7.

Proteomic databases

PaxDbiQ8C5S7.
PeptideAtlasiQ8C5S7.
PRIDEiQ8C5S7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002395; ENSMUSP00000002395; ENSMUSG00000002324.
GeneIDi56739.
KEGGimmu:56739.
UCSCiuc007tzo.2. mouse.

Organism-specific databases

CTDi9985.
MGIiMGI:1929645. Rec8.

Phylogenomic databases

eggNOGiKOG1213. Eukaryota.
ENOG410XRB4. LUCA.
GeneTreeiENSGT00390000011379.
HOGENOMiHOG000154087.
HOVERGENiHBG056766.
InParanoidiQ8C5S7.
KOiK13054.
OMAiETQISRK.
OrthoDBiEOG091G0CXC.
PhylomeDBiQ8C5S7.
TreeFamiTF338144.

Enzyme and pathway databases

ReactomeiR-MMU-1221632. Meiotic synapsis.
R-MMU-1221633. Meiotic Synapsis.

Miscellaneous databases

PROiQ8C5S7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002324.
CleanExiMM_REC8.
GenevisibleiQ8C5S7. MM.

Family and domain databases

Gene3Di1.10.10.580. 1 hit.
InterProiIPR023093. Rad21/Rec8_C.
IPR006909. Rad21/Rec8_C_eu.
IPR006910. Rad21_Rec8_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04824. Rad21_Rec8. 1 hit.
PF04825. Rad21_Rec8_N. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiREC8_MOUSE
AccessioniPrimary (citable) accession number: Q8C5S7
Secondary accession number(s): Q3UIN9, Q9JK52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.