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Protein

Meiotic recombination protein REC8 homolog

Gene

Rec8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.4 Publications

GO - Biological processi

  • double-strand break repair via homologous recombination Source: MGI
  • fertilization Source: MGI
  • male meiosis I Source: MGI
  • meiotic nuclear division Source: MGI
  • oocyte maturation Source: MGI
  • seminiferous tubule development Source: MGI
  • spermatid development Source: MGI
  • synapsis Source: MGI
  • synaptonemal complex assembly Source: MGI
Complete GO annotation...

Keywords - Biological processi

Chromosome partition, Meiosis

Enzyme and pathway databases

ReactomeiR-MMU-1221633. Meiotic Synapsis.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein REC8 homolog
Alternative name(s):
Cohesin Rec8p
Gene namesi
Name:Rec8
Synonyms:Mei8, Rec8L1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1929645. Rec8.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome Source: MGI
  • condensed nuclear chromosome, centromeric region Source: MGI
  • condensed nuclear chromosome kinetochore Source: MGI
  • lateral element Source: MGI
  • male germ cell nucleus Source: MGI
  • meiotic cohesin complex Source: MGI
  • nuclear meiotic cohesin complex Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
  • synaptonemal complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display a high mortality rate, both during embryogenesis and after birth, germ cell failure and sterility. Mutant females exhibit ovarian dysgenesis and lack ovarian follicles at reproductive maturity. Affected males have small testes due to arrest of spermatogenesis during meiotic prophase I. Early chromosome pairing appears normal but synapsis occurs between sister chromatids rather than between homologous chromosomes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 591591Meiotic recombination protein REC8 homologPRO_0000097879Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei149 – 1491PhosphoserineCombined sources
Modified residuei164 – 1641PhosphothreonineBy similarity
Modified residuei192 – 1921PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8C5S7.
PaxDbiQ8C5S7.
PRIDEiQ8C5S7.

PTM databases

PhosphoSiteiQ8C5S7.

Expressioni

Tissue specificityi

Expressed primarily in the gonads. In the testis, expressed in pachytene spermatocytes and in spermatids. Not expressed in spermatogonia or somatic cells. In the ovary, expressed only in oocytes. Low levels also detected in a number of somatic tissues including thymus, lung, liver, kidney and small intestine.4 Publications

Developmental stagei

Expressed from 2 weeks postpartum (at protein level).1 Publication

Gene expression databases

BgeeiQ8C5S7.
CleanExiMM_REC8.
GenevisibleiQ8C5S7. MM.

Interactioni

Subunit structurei

Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51.1 Publication

Protein-protein interaction databases

BioGridi208153. 1 interaction.
DIPiDIP-60730N.
IntActiQ8C5S7. 4 interactions.
STRINGi10090.ENSMUSP00000002395.

Structurei

3D structure databases

ProteinModelPortaliQ8C5S7.
SMRiQ8C5S7. Positions 19-101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the rad21 family.Curated

Phylogenomic databases

eggNOGiKOG1213. Eukaryota.
ENOG410XRB4. LUCA.
GeneTreeiENSGT00390000011379.
HOGENOMiHOG000154087.
HOVERGENiHBG056766.
InParanoidiQ8C5S7.
KOiK13054.
OMAiETQISRK.
OrthoDBiEOG7K6PV2.
PhylomeDBiQ8C5S7.
TreeFamiTF338144.

Family and domain databases

Gene3Di1.10.10.580. 1 hit.
InterProiIPR023093. Rad21/Rec8_C.
IPR006909. Rad21/Rec8_C_eu.
IPR006910. Rad21_Rec8_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04824. Rad21_Rec8. 1 hit.
PF04825. Rad21_Rec8_N. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8C5S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYYPNVLQR HTGCFATIWL AATRGSRLVK REYLNVNVVK TCEEILNYVL
60 70 80 90 100
VRVQPPVAGL PRPRFSLYLS AQLQIGVIRV YFQQCQYLVE DIQHILEHLH
110 120 130 140 150
RAQLRIRIDM EEADLPSLLL PNCLAMMETL EDAPEPFFGK MSVDPRLPSP
160 170 180 190 200
FDIPQIRHLL EAATPEKTRK ETLPEATPDP RKPDRTLATV QSPEVITLQE
210 220 230 240 250
AEPIRMLQIE GEQDLPEISR GDLELLIAEK DDAILLEERQ RGRLLRQRRA
260 270 280 290 300
SLPLDESREE PRALEGAGLV SALSPPAPAQ VEGIQEALPG QVFPPEVQKM
310 320 330 340 350
TGWEPGALLT EVTPPQELRL PAPPSTEKRL PSLQRPLPRR HRRRQLLFWD
360 370 380 390 400
KETQISREKF EEQLQTGAHC WEYPVAQPPK RMLTSPAELF RTPTLSGWLP
410 420 430 440 450
PELLGLWTHC AQVPQRMLRQ RPQLETEETV EEERAADEEE RRKTEALSEI
460 470 480 490 500
EVLREAQEPS GPLMLSSELS LEAAEDEKSR TSLIPPEWWA WSEEGQPEPP
510 520 530 540 550
ALPMLPELPE VPMEMPPRPE LSSEAVLRAV ALKLQANKEL DFSSLVPPLS
560 570 580 590
PRKLASRVFY LLLVLSTQKI LLVEQQKPYG PLLIRPGPKF P
Length:591
Mass (Da):67,425
Last modified:March 1, 2003 - v1
Checksum:i622986F564735700
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491V → E in AAF69524 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262055 mRNA. Translation: AAF69524.1.
AK077167 mRNA. Translation: BAC36657.1.
AK146832 mRNA. Translation: BAE27467.1.
BC052155 mRNA. Translation: AAH52155.1.
CCDSiCCDS27120.1.
RefSeqiNP_064386.2. NM_020002.3.
UniGeneiMm.23149.

Genome annotation databases

EnsembliENSMUST00000002395; ENSMUSP00000002395; ENSMUSG00000002324.
GeneIDi56739.
KEGGimmu:56739.
UCSCiuc007tzo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF262055 mRNA. Translation: AAF69524.1.
AK077167 mRNA. Translation: BAC36657.1.
AK146832 mRNA. Translation: BAE27467.1.
BC052155 mRNA. Translation: AAH52155.1.
CCDSiCCDS27120.1.
RefSeqiNP_064386.2. NM_020002.3.
UniGeneiMm.23149.

3D structure databases

ProteinModelPortaliQ8C5S7.
SMRiQ8C5S7. Positions 19-101.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208153. 1 interaction.
DIPiDIP-60730N.
IntActiQ8C5S7. 4 interactions.
STRINGi10090.ENSMUSP00000002395.

PTM databases

PhosphoSiteiQ8C5S7.

Proteomic databases

MaxQBiQ8C5S7.
PaxDbiQ8C5S7.
PRIDEiQ8C5S7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002395; ENSMUSP00000002395; ENSMUSG00000002324.
GeneIDi56739.
KEGGimmu:56739.
UCSCiuc007tzo.2. mouse.

Organism-specific databases

CTDi9985.
MGIiMGI:1929645. Rec8.

Phylogenomic databases

eggNOGiKOG1213. Eukaryota.
ENOG410XRB4. LUCA.
GeneTreeiENSGT00390000011379.
HOGENOMiHOG000154087.
HOVERGENiHBG056766.
InParanoidiQ8C5S7.
KOiK13054.
OMAiETQISRK.
OrthoDBiEOG7K6PV2.
PhylomeDBiQ8C5S7.
TreeFamiTF338144.

Enzyme and pathway databases

ReactomeiR-MMU-1221633. Meiotic Synapsis.

Miscellaneous databases

NextBioi313236.
PROiQ8C5S7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C5S7.
CleanExiMM_REC8.
GenevisibleiQ8C5S7. MM.

Family and domain databases

Gene3Di1.10.10.580. 1 hit.
InterProiIPR023093. Rad21/Rec8_C.
IPR006909. Rad21/Rec8_C_eu.
IPR006910. Rad21_Rec8_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04824. Rad21_Rec8. 1 hit.
PF04825. Rad21_Rec8_N. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "mrec8, a meiotic homolog of the rad21 cohesin family."
    Beasley M., Warren W., McKay M.J.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. "Rec8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans."
    Parisi S., McKay M.J., Molnar M., Thompson M.A., van der Spek P.J., van Drunen-Schoenmaker E., Kanaar R., Lehmann E., Hoeijmakers J.H.J., Kohli J.
    Mol. Cell. Biol. 19:3515-3528(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Analyses of mRNA expression patterns of cohesin subunits Rad21 and Rec8 in mice: germ cell-specific expression of rec8 mRNA in both male and female mice."
    Lee J., Yokota T., Yamashita M.
    Zool. Sci. 19:539-544(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis."
    Lee J., Iwai T., Yokota T., Yamashita M.
    J. Cell Sci. 116:2781-2790(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SMC1B; SMC3 AND SYCP3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION.
  8. "Positional cloning and characterization of mouse mei8, a disrupted allelle of the meiotic cohesin Rec8."
    Bannister L.A., Reinholdt L.G., Munroe R.J., Schimenti J.C.
    Genesis 40:184-194(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Absence of mouse REC8 cohesin promotes synapsis of sister chromatids in meiosis."
    Xu H., Beasley M.D., Warren W.D., van der Horst G.T.J., McKay M.J.
    Dev. Cell 8:949-961(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  10. "Loss of Rec8 from chromosome arm and centromere region is required for homologous chromosome separation and sister chromatid separation, respectively, in mammalian meiosis."
    Lee J., Okada K., Ogushi S., Miyano T., Miyake M., Yamashita M.
    Cell Cycle 5:1448-1455(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  12. "Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis."
    Fukuda T., Pratto F., Schimenti J.C., Turner J.M., Camerini-Otero R.D., Hoeoeg C.
    PLoS Genet. 8:E1002485-E1002485(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.

Entry informationi

Entry nameiREC8_MOUSE
AccessioniPrimary (citable) accession number: Q8C5S7
Secondary accession number(s): Q3UIN9, Q9JK52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: March 16, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.