Nos3

Functionⁱ

Produces nitric oxide (NO).UniRule annotation

Catalytic activityⁱ

2 L-arginine + 3 NADPH + 4 O₂ = 2 L-citrulline + 2 nitric oxide + 3 NADP⁺ + 4 H₂O.UniRule annotation

Cofactorⁱ

Protein has several cofactor binding sites:

FAD
UniRule annotation
Automatic assertion according to rulesⁱ
- PIRNR:PIRNR000333
Note: Binds 1 FAD.
UniRule annotation
Automatic assertion according to rulesⁱ
- PIRNR:PIRNR000333
FMN
UniRule annotation
Automatic assertion according to rulesⁱ
- PIRNR:PIRNR000333
Note: Binds 1 FMN.
UniRule annotation
Automatic assertion according to rulesⁱ
- PIRNR:PIRNR000333
heme
UniRule annotation
Automatic assertion according to rulesⁱ
- PIRNR:PIRNR000333

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Metal bindingⁱ	183	Iron (heme axial ligand)UniRule annotation		1

GO - Molecular functionⁱ

calmodulin binding Source: UniProtKB-KW
flavin adenine dinucleotide binding Source: InterPro
FMN binding Source: InterPro
heme binding Source: InterPro
iron ion binding Source: InterPro
NADP binding Source: InterPro
nitric-oxide synthase activity Source: UniProtKB-EC

Complete GO annotation...

GO - Biological processⁱ

nitric oxide biosynthetic process Source: InterPro

Complete GO annotation...

Keywordsⁱ

Molecular function	Calmodulin-bindingUniRule annotation, OxidoreductaseUniRule annotation
Ligand	FADUniRule annotation, Flavoprotein, FMNUniRule annotation, HemeUniRule annotationSAAS annotation, Iron, Metal-binding, NADPUniRule annotation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Nitric oxide synthaseUniRule annotation (EC:1.14.13.39UniRule annotation)
Gene namesⁱ	Name:Nos3Imported
Organismⁱ	Mus musculus (Mouse)Imported
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus

Organism-specific databases

MGIⁱ	MGI:97362. Nos3.

PTM / Processingⁱ

Proteomic databases

PaxDbⁱ	Q8C5P3.

PaxDbⁱ

Q8C5P3.

Expressionⁱ

Gene expression databases

Genevisibleⁱ	Q8C5P3. MM.

Genevisibleⁱ

Q8C5P3. MM.

Interactionⁱ

GO - Molecular functionⁱ

calmodulin binding Source: UniProtKB-KW

Complete GO annotation...

Protein-protein interaction databases

IntActⁱ	Q8C5P3. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-4114447.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8C5P3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	519 – 702	Flavodoxin-likeInterPro annotationAdd BLAST		184
Domainⁱ	755 – 1001	FAD-binding FR-typeInterPro annotationAdd BLAST		247

Sequence similaritiesⁱ

Belongs to the NOS family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	KOG1158. Eukaryota. COG0369. LUCA. COG4362. LUCA.
HOVERGENⁱ	HBG000159.
KEGG Orthology (KO) More...KOⁱ	K13242.

Family and domain databases

Gene3Dⁱ	3.40.50.360. 1 hit.
InterProⁱ	View protein in InterPro IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR029039. Flavoprotein-like_dom. IPR012144. NOS_euk. IPR004030. NOS_N. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF02898. NO_synthase. 1 hit.
PIRSFⁱ	PIRSF000333. NOS. 1 hit.
PRINTSⁱ	PR00369. FLAVODOXIN. PR00371. FPNCR.
SUPFAMⁱ	SSF52218. SSF52218. 1 hit. SSF56512. SSF56512. 1 hit. SSF63380. SSF63380. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q8C5P3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGNLKSVGQE PGPPCGLGLG LGLGLCGKQG PASPAPEPSQ APAPPSPTRP 
        60         70         80         90        100
APDHSPPLTR PPDGPRFPRV KNWEVGSITY DTLSAQAQQD GPCTSRRCLG 
       110        120        130        140        150
SLVFPRKLQS RPTQGPSPTE QLLGQARDFI NQYYNSIKRS GSQAHEQRLQ 
       160        170        180        190        200
EVEAEVAATG TYQLRESELV FGAKQAWRNA PRCVGRIQWG KLQVFDARDC 
       210        220        230        240        250
RTAQEMFTYI CNHIKYATNR GNLRSAITVF PQRCPGRGDF RIWNSQLIRY 
       260        270        280        290        300
AGYRQQDGSV RGDPANVEIT ELCIQHGWTP GNGRFDVLPL LLQAPDEPPE 
       310        320        330        340        350
LFTLPPEMVL EVPLEHPTLE WFAALGLRWY ALPAVSNMLL EIGGLEFPAA 
       360        370        380        390        400
PFSGWYMSSE IGMRDLCDPH RYNILEDVAV CMDLDTRTTS SLWKDKAAVE 
       410        420        430        440        450
INVAVLHSYQ LAKVTIVDHH AATASFMKHL ENEQKARGGC PADWAWIVPP 
       460        470        480        490        500
ISGSLTPVFH QEMVNYFLSP AFRYQPDPWK GSAAKGAGIT RKKTFKEVAN 
       510        520        530        540        550
AVKVSASLMG TVMAKRVKAT ILYGSEAGRA QSYAQQLGRL FRKAFDPRVL 
       560        570        580        590        600
CMDEYDVVSL EHEALVLVVT STFGNGDPPE NGESFAAALM EMSGPYNSSP 
       610        620        630        640        650
RPEQHKSYKI RFNSVSCSDP LVSSWRRKRK ESSNTDSAGA LGTLRFCVFG 
       660        670        680        690        700
LGSRAYPHFC AFARAVDTRL EELGGERLLQ LGQGDELCGQ EEAFRGWAQA 
       710        720        730        740        750
AFQAACETFC VGEDAKAAAR DIFSPKRSWK RQRYRLSTQA ESLQLLPGLT 
       760        770        780        790        800
HVHRRKMFQA TILSVENLQS SKSTRATILV RLDTGGQEGL QYQPGDHIGV 
       810        820        830        840        850
CPPNRPGLVE ALLSRVEDPP PSTEPVAVEQ LEKGSPGGPP PGWVRDPRLP 
       860        870        880        890        900
PCTLRQALTY FLDITSPPSP RLLRLLSTLA EESSEQQELE ALSQDPRRYE 
       910        920        930        940        950
EWKWFSCPTL LEVLEQFPSV ALPAPLILTQ LPLLQPRYYS VSSAPSAHPG 
       960        970        980        990       1000
EIHLTIAVLA YRTQDGLGPL HYGVCSTWMS QLKAGDPVPC FIRGAPSFRL 
      1010       1020       1030       1040       1050
PPDPNLPCIL VGPGTGIAPF RGFWQDRLHD IEIKGLQPAP MTLVFGCRCS 
      1060       1070       1080       1090       1100
QLDHLYRDEV LDAQQRGVFG QVLTAFSRDP GSPKTYVQDL LRTELAAEVH 
      1110       1120       1130       1140       1150
RVLCLEQGHM FVCGDVTMAT SVLQTVQRIL ATEGGMELDE AGDVIGVLRD 
      1160       1170       1180       1190       1200
QQRYHEDIFG LTLRTQEVTS RIRTQSFSLQ ERQLRGAVPW SFDPPGPEIP 

GS

Length:1,202

Mass (Da):132,872

Last modified:March 1, 2003 - v1

Checksum:ⁱF3CC089611D24B1E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK077896 mRNA. Translation: BAC37052.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_032739.3. NM_008713.4.
UniGeneⁱ	Mm.258415.

Genome annotation databases

GeneIDⁱ	18127.
KEGGⁱ	mmu:18127.

Similar proteinsⁱ

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q8C5P3	P29474 A0A090N8H8 UPI0003F153AB S7NFW7 F6REQ2 Q8SPL6 UPI0003ADF414 F1PV05 M3Y867 G9KDP7 +178	Homo sapiens (Human) Chrysochloris asiatica (Cape golden mole) Myotis brandtii (Brandt's bat) Equus caballus (Horse) Canis lupus familiaris (Dog) (Canis familiaris) Mustela putorius furo (European domestic ferret) (Mustela furo) Ochotona curzoniae (Black-lipped pika) Octodon degus (Degu) (Sciurus degus) Oryctolagus cuniculus (Rabbit) Ictidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus tridecemlineatus) And more	1,203	UniRef90_P29474	Cluster: Nitric oxide synthase, endothelial	189

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q8C5P3	P29474 A0A090N8H8 UPI0003F153AB F6REQ2 Q8SPL6 S7NFW7 UPI0003ADF414 F1PV05 UPI00062A937F UPI000333CB94 +220	Homo sapiens (Human) Chrysochloris asiatica (Cape golden mole) Equus caballus (Horse) Myotis brandtii (Brandt's bat) Canis lupus familiaris (Dog) (Canis familiaris) Octodon degus (Degu) (Sciurus degus) Ochotona curzoniae (Black-lipped pika) Mustela putorius furo (European domestic ferret) (Mustela furo) Ictidomys tridecemlineatus (Thirteen-lined ground squirrel) (Spermophilus tridecemlineatus) Oryctolagus cuniculus (Rabbit) And more	1,203	UniRef50_P29474	Cluster: Nitric oxide synthase, endothelial	231

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK077896 mRNA. Translation: BAC37052.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_032739.3. NM_008713.4.
UniGeneⁱ	Mm.258415.

3D structure databases

ProteinModelPortalⁱ	Q8C5P3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	Q8C5P3. 1 interactor.
Molecular INTeraction database More...MINTⁱ	MINT-4114447.

Proteomic databases

PaxDbⁱ	Q8C5P3.

PaxDbⁱ

Q8C5P3.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	18127.
KEGGⁱ	mmu:18127.

Organism-specific databases

CTDⁱ	4846.
MGIⁱ	MGI:97362. Nos3.

Phylogenomic databases

eggNOGⁱ	KOG1158. Eukaryota. COG0369. LUCA. COG4362. LUCA.
HOVERGENⁱ	HBG000159.
KEGG Orthology (KO) More...KOⁱ	K13242.

Miscellaneous databases

SOURCEⁱ	Search...

SOURCEⁱ

Search...

Gene expression databases

Genevisibleⁱ	Q8C5P3. MM.

Genevisibleⁱ

Q8C5P3. MM.

Family and domain databases

Gene3Dⁱ	3.40.50.360. 1 hit.
InterProⁱ	View protein in InterPro IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR029039. Flavoprotein-like_dom. IPR012144. NOS_euk. IPR004030. NOS_N. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. PF02898. NO_synthase. 1 hit.
PIRSFⁱ	PIRSF000333. NOS. 1 hit.
PRINTSⁱ	PR00369. FLAVODOXIN. PR00371. FPNCR.
SUPFAMⁱ	SSF52218. SSF52218. 1 hit. SSF56512. SSF56512. 1 hit. SSF63380. SSF63380. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. PS60001. NOS. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	Q8C5P3_MOUSE
Accessionⁱ	Q8C5P3Primary (citable) accession number: Q8C5P3
Entry historyⁱ	Integrated into UniProtKB/TrEMBL:	March 1, 2003
	Last sequence update:	March 1, 2003
	Last modified:	August 30, 2017
	This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Unreviewed (UniProtKB/TrEMBL)

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UniProtKB - Q8C5P3 (Q8C5P3_MOUSE)

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Nitric oxide synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ