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Protein

CCR4-NOT transcription complex subunit 2

Gene

Cnot2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specificly involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of emryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 2
Alternative name(s):
CCR4-associated factor 2
Gene namesi
Name:Cnot2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1919318. Cnot2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540CCR4-NOT transcription complex subunit 2PRO_0000198332Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931PhosphothreonineBy similarity
Modified residuei126 – 1261PhosphoserineBy similarity
Modified residuei157 – 1571PhosphoserineCombined sources
Modified residuei165 – 1651PhosphoserineCombined sources
Modified residuei169 – 1691PhosphoserineCombined sources
Modified residuei242 – 2421PhosphoserineBy similarity
Modified residuei274 – 2741PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8C5L3.
MaxQBiQ8C5L3.
PaxDbiQ8C5L3.
PRIDEiQ8C5L3.

PTM databases

iPTMnetiQ8C5L3.
PhosphoSiteiQ8C5L3.

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells and in inner cell mass (ICM) of the blastocyst.1 Publication

Gene expression databases

BgeeiQ8C5L3.
CleanExiMM_CNOT2.
ExpressionAtlasiQ8C5L3. baseline and differential.
GenevisibleiQ8C5L3. MM.

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT3. Interacts with NCOR1, NCOR2. HDAC3 and GPS2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215128. 19 interactions.
IntActiQ8C5L3. 19 interactions.
STRINGi10090.ENSMUSP00000100902.

Structurei

3D structure databases

ProteinModelPortaliQ8C5L3.
SMRiQ8C5L3. Positions 350-540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Phylogenomic databases

eggNOGiKOG2151. Eukaryota.
COG5601. LUCA.
GeneTreeiENSGT00390000001285.
HOGENOMiHOG000246523.
HOVERGENiHBG051041.
InParanoidiQ8C5L3.
KOiK12605.
OMAiNIPSGMV.
OrthoDBiEOG77127J.
PhylomeDBiQ8C5L3.
TreeFamiTF313102.

Family and domain databases

InterProiIPR007282. NOT.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C5L3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVRTDGHTLS EKRNYQVTNS MFGASRKKFV EGVDSDYHDE NMYYSQSSMF
60 70 80 90 100
PHRSEKDMLA SPSTSGQLSQ FGASLYGQQS ALGLPMRGMS NNTPQLNRSL
110 120 130 140 150
SQGTQLPSHV TPTTGVPTMS LHTPPSPSRG ILPMNPRNMM NHSQVGQGIG
160 170 180 190 200
IPSRTNSMSS SGLGSPNRSS PSIICMPKQQ PSRQPFTVNS MSGFGMNRNQ
210 220 230 240 250
AFGMNNSLSS NIFNGTDGSE NVTGLDLSDF PALADRNRRE GSGNPTPLIN
260 270 280 290 300
PLAGRAPYVG MVTKPANEQS QDFSIHNEDF PALPGSSYKD PTSSNDDSKS
310 320 330 340 350
NLSTSGKTTS STDGPKFPGD KSSTTQNNNQ QKKGIQVLPD GRVTNIPQGM
360 370 380 390 400
VTDQFGMIGL LTFIRAAETD PGMVHLALGS DLTTLGLNLN SPENLYPKFA
410 420 430 440 450
SPWASSPCRP QDIDFHVPSE YLTNIHIRDK LAAIKLGRYG EDLLFYLYYM
460 470 480 490 500
NGGDVLQLLA AVELFNRDWR YHKEERVWIT RAPGMEPTMK TNTYERGTYY
510 520 530 540
FFDCLNWRKV AKEFHLEYDK LEERPHLPST FNYNPAQQAF
Length:540
Mass (Da):59,711
Last modified:April 13, 2004 - v2
Checksum:iC5185262B5E3C78C
GO
Isoform 2 (identifier: Q8C5L3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MVRTDGHTLSEKRNY → MLKEVA

Note: No experimental confirmation available.
Show »
Length:531
Mass (Da):58,594
Checksum:i242DC8CF90C700E6
GO
Isoform 3 (identifier: Q8C5L3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Note: No experimental confirmation available.
Show »
Length:455
Mass (Da):50,224
Checksum:i9778467D3A90FB8E
GO

Sequence cautioni

The sequence AAH43133.1 differs from that shown. Reason: Frameshift at position 1. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti148 – 1481G → S in BAC37134 (PubMed:16141072).Curated
Sequence conflicti426 – 4261H → Y in AAH43133 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8585Missing in isoform 3. 1 PublicationVSP_009917Add
BLAST
Alternative sequencei1 – 1515MVRTD…EKRNY → MLKEVA in isoform 2. 1 PublicationVSP_009918Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011231 mRNA. Translation: BAB27481.1.
AK078121 mRNA. Translation: BAC37134.1.
AK149767 mRNA. Translation: BAE29072.1.
BC043133 mRNA. Translation: AAH43133.1. Frameshift.
BC063105 mRNA. Translation: AAH63105.1.
BC065171 mRNA. Translation: AAH65171.1.
CCDSiCCDS36064.1. [Q8C5L3-1]
RefSeqiNP_001032935.2. NM_001037846.3. [Q8C5L3-1]
NP_001032936.2. NM_001037847.2.
NP_001032937.1. NM_001037848.3.
NP_082358.2. NM_028082.2.
XP_006514238.1. XM_006514175.2. [Q8C5L3-1]
XP_006514240.1. XM_006514177.2. [Q8C5L3-2]
XP_006514242.1. XM_006514179.2. [Q8C5L3-2]
XP_006514243.1. XM_006514180.1. [Q8C5L3-2]
XP_006514244.1. XM_006514181.1. [Q8C5L3-2]
XP_006514246.1. XM_006514183.2. [Q8C5L3-3]
XP_006514247.1. XM_006514184.2. [Q8C5L3-3]
UniGeneiMm.351553.

Genome annotation databases

EnsembliENSMUST00000105265; ENSMUSP00000100900; ENSMUSG00000020166. [Q8C5L3-3]
ENSMUST00000105267; ENSMUSP00000100902; ENSMUSG00000020166. [Q8C5L3-1]
ENSMUST00000169921; ENSMUSP00000132152; ENSMUSG00000020166. [Q8C5L3-1]
GeneIDi72068.
KEGGimmu:72068.
UCSCiuc007hcb.2. mouse. [Q8C5L3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011231 mRNA. Translation: BAB27481.1.
AK078121 mRNA. Translation: BAC37134.1.
AK149767 mRNA. Translation: BAE29072.1.
BC043133 mRNA. Translation: AAH43133.1. Frameshift.
BC063105 mRNA. Translation: AAH63105.1.
BC065171 mRNA. Translation: AAH65171.1.
CCDSiCCDS36064.1. [Q8C5L3-1]
RefSeqiNP_001032935.2. NM_001037846.3. [Q8C5L3-1]
NP_001032936.2. NM_001037847.2.
NP_001032937.1. NM_001037848.3.
NP_082358.2. NM_028082.2.
XP_006514238.1. XM_006514175.2. [Q8C5L3-1]
XP_006514240.1. XM_006514177.2. [Q8C5L3-2]
XP_006514242.1. XM_006514179.2. [Q8C5L3-2]
XP_006514243.1. XM_006514180.1. [Q8C5L3-2]
XP_006514244.1. XM_006514181.1. [Q8C5L3-2]
XP_006514246.1. XM_006514183.2. [Q8C5L3-3]
XP_006514247.1. XM_006514184.2. [Q8C5L3-3]
UniGeneiMm.351553.

3D structure databases

ProteinModelPortaliQ8C5L3.
SMRiQ8C5L3. Positions 350-540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215128. 19 interactions.
IntActiQ8C5L3. 19 interactions.
STRINGi10090.ENSMUSP00000100902.

PTM databases

iPTMnetiQ8C5L3.
PhosphoSiteiQ8C5L3.

Proteomic databases

EPDiQ8C5L3.
MaxQBiQ8C5L3.
PaxDbiQ8C5L3.
PRIDEiQ8C5L3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105265; ENSMUSP00000100900; ENSMUSG00000020166. [Q8C5L3-3]
ENSMUST00000105267; ENSMUSP00000100902; ENSMUSG00000020166. [Q8C5L3-1]
ENSMUST00000169921; ENSMUSP00000132152; ENSMUSG00000020166. [Q8C5L3-1]
GeneIDi72068.
KEGGimmu:72068.
UCSCiuc007hcb.2. mouse. [Q8C5L3-1]

Organism-specific databases

CTDi4848.
MGIiMGI:1919318. Cnot2.

Phylogenomic databases

eggNOGiKOG2151. Eukaryota.
COG5601. LUCA.
GeneTreeiENSGT00390000001285.
HOGENOMiHOG000246523.
HOVERGENiHBG051041.
InParanoidiQ8C5L3.
KOiK12605.
OMAiNIPSGMV.
OrthoDBiEOG77127J.
PhylomeDBiQ8C5L3.
TreeFamiTF313102.

Enzyme and pathway databases

ReactomeiR-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Miscellaneous databases

ChiTaRSiCnot2. mouse.
PROiQ8C5L3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C5L3.
CleanExiMM_CNOT2.
ExpressionAtlasiQ8C5L3. baseline and differential.
GenevisibleiQ8C5L3. MM.

Family and domain databases

InterProiIPR007282. NOT.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Bone marrow, Embryo and Medulla oblongata.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain and Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157; SER-165 AND SER-169, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  4. "Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation."
    Zheng X., Dumitru R., Lackford B.L., Freudenberg J.M., Singh A.P., Archer T.K., Jothi R., Hu G.
    Stem Cells 30:910-922(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCNOT2_MOUSE
AccessioniPrimary (citable) accession number: Q8C5L3
Secondary accession number(s): Q3UE39, Q80YA5, Q9D0P1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: July 6, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.