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Protein

Lipoprotein lipase

Gene

Lpl

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.UniRule annotation

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei159 – 1591NucleophileUniRule annotation
Active sitei183 – 1831Charge relay systemUniRule annotation
Active sitei268 – 2681Charge relay systemUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Lipid degradationUniRule annotation, Lipid metabolism

Keywords - Ligandi

Heparin-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein lipaseUniRule annotation (EC:3.1.1.34UniRule annotation)
Short name:
LPLUniRule annotation
Gene namesi
Name:LplImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:96820. Lpl.

Subcellular locationi

  • Cell membrane UniRule annotation; Lipid-anchorGPI-anchor UniRule annotation
  • Secreted UniRule annotation

  • Note: Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membraneUniRule annotation, ChylomicronUniRule annotation, Membrane, SecretedUniRule annotationSAAS annotation, VLDLUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020UniRule annotationAdd
BLAST
Chaini21 – 474454Lipoprotein lipaseUniRule annotationPRO_5001373986Add
BLAST

Post-translational modificationi

Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation, Glycoprotein, GPI-anchorUniRule annotation, Lipoprotein, NitrationUniRule annotation

Expressioni

Gene expression databases

GenevestigatoriQ8C562.

Interactioni

Subunit structurei

Homodimer. Interacts with APOC2; the interaction activates LPL activity in the presence of lipids.UniRule annotation

Protein-protein interaction databases

IntActiQ8C562. 2 interactions.
MINTiMINT-4114390.

Structurei

3D structure databases

ProteinModelPortaliQ8C562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.UniRule annotation
Contains 1 PLAT domain.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

HOVERGENiHBG002259.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR008976. Lipase_LipOase.
IPR013818. Lipase_N.
IPR002330. Lipo_Lipase.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PTHR11610:SF3. PTHR11610:SF3. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00822. LIPOLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03230. lipo_lipase. 1 hit.
PROSITEiPS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C562-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESKALLLVV LGVWLQSLTA FRGGVAAEDA GRDFSDIESK FALRTPEDTA
60 70 80 90 100
EDTCHLIPGL ADSVSNCHFN HSSKTFVVIH GWTVTGMYES WVPKLVAALY
110 120 130 140 150
KREPDSNVIV VDWLYRAQQH YPVSAGYTKL VGNDVARFIN WMEEEFNYPL
160 170 180 190 200
DNVHLLGYSL GAHAAGVAGS LTNKKVNRIT GLDPAGPNFE YAEAPSRLSP
210 220 230 240 250
DDADFVDVLH TFTRGSPGRS IGIQKPVGHV DIYPNGGTFQ PGCNIGEAIR
260 270 280 290 300
VIAERGLGDV DQLVKCSHER SIHLFIDSLL NEENPSKAYR CNSKEAFEKG
310 320 330 340 350
LCLSCRKNRC NNLGYEINKV RAKRSSKMYL KTRSQMPYKV FHYQVKIHFS
360 370 380 390 400
GTEDGKQHNQ AFEISLYGTV AESENIPFTL PEVSTNKTYS FLIYTEVDIG
410 420 430 440 450
ELLMMKLKWI SDSYFSWPDW WSSPSFVIER IRVKAGETQK KVIFCAREKV
460 470
SHLQKGKDSA VFVKCHDKSL KKSG
Length:474
Mass (Da):53,167
Last modified:March 1, 2003 - v1
Checksum:i5173F44A8BC003DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK079446 mRNA. Translation: BAC37646.1.
UniGeneiMm.1514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK079446 mRNA. Translation: BAC37646.1.
UniGeneiMm.1514.

3D structure databases

ProteinModelPortaliQ8C562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8C562. 2 interactions.
MINTiMINT-4114390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:96820. Lpl.

Phylogenomic databases

HOVERGENiHBG002259.

Miscellaneous databases

ChiTaRSiLpl. mouse.
SOURCEiSearch...

Gene expression databases

GenevestigatoriQ8C562.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR008976. Lipase_LipOase.
IPR013818. Lipase_N.
IPR002330. Lipo_Lipase.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PTHR11610:SF3. PTHR11610:SF3. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00822. LIPOLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03230. lipo_lipase. 1 hit.
PROSITEiPS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "High-efficiency full-length cDNA cloning."
    Carninci P., Hayashizaki Y.
    Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: VaginaImported.
  2. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
    Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
    Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: VaginaImported.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: VaginaImported.
  4. "Functional annotation of a full-length mouse cDNA collection."
    The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium
    Nature 409:685-690(2001)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: VaginaImported.
  5. "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
    The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
    Nature 420:563-573(2002)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: VaginaImported.
  6. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: VaginaImported.
  7. "The Transcriptional Landscape of the Mammalian Genome."
    The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
    Science 309:1559-1563(2005)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: VaginaImported.
  8. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: VaginaImported.

Entry informationi

Entry nameiQ8C562_MOUSE
AccessioniPrimary (citable) accession number: Q8C562
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2003
Last sequence update: March 1, 2003
Last modified: May 27, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.