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Protein

Negative elongation factor B

Gene

Nelfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Binds RNA which may help to stabilize the NELF complex on nucleic acid. May be able to induce chromatin unfolding (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor B
Short name:
NELF-B
Alternative name(s):
Cofactor of BRCA1
Gene namesi
Name:Nelfb
Synonyms:Cobra1
ORF Names:MNCb-5210
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1931035. Nelfb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191301 – 580Negative elongation factor BAdd BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei519N6-acetyllysineBy similarity1
Modified residuei557PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8C4Y3.
MaxQBiQ8C4Y3.
PaxDbiQ8C4Y3.
PeptideAtlasiQ8C4Y3.
PRIDEiQ8C4Y3.

PTM databases

iPTMnetiQ8C4Y3.
PhosphoSitePlusiQ8C4Y3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000013465.
CleanExiMM_COBRA1.
GenevisibleiQ8C4Y3. MM.

Interactioni

Subunit structurei

The NELF complex is composed of NELFA, NELFB, NELFCD and NELFE; the N-terminus of NELFB binds to the NELFA:NELFCD subcomplex. Interacts with the first BRCT repeat of BRCA1 (By similarity). Interacts with KIAA1191 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057731.

Structurei

3D structure databases

SMRiQ8C4Y3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NELF-B family.Curated

Phylogenomic databases

eggNOGiENOG410IEBF. Eukaryota.
ENOG410YK9P. LUCA.
HOGENOMiHOG000231489.
HOVERGENiHBG052596.
InParanoidiQ8C4Y3.
KOiK15180.
OrthoDBiEOG091G04WH.
PhylomeDBiQ8C4Y3.
TreeFamiTF324620.

Family and domain databases

InterProiView protein in InterPro
IPR010405. COBRA1.
PANTHERiPTHR13503. PTHR13503. 1 hit.
PfamiView protein in Pfam
PF06209. COBRA1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8C4Y3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF
60 70 80 90 100
LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS
110 120 130 140 150
FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW
160 170 180 190 200
QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV
210 220 230 240 250
VQKLTQMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV
260 270 280 290 300
SDICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD
310 320 330 340 350
LSMILCDPFA INTLSLSTIR HLQELVSQET LPRDSPDLLL LLRLLALGQG
360 370 380 390 400
AWDLIDSQVF KEPKMEAELI TKFLPMLMSL VVDDFTFNVD QKLPAEEKAS
410 420 430 440 450
VTYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE
460 470 480 490 500
TFGDLAFSDI FLHLLTGSLV LLADEFALED FCSSLFDGFF LTASPRKENV
510 520 530 540 550
HRHVLRLLLH LHARVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ
560 570 580
LDHRKPSPTQ AAETPALDLP LPSVPAPATL
Length:580
Mass (Da):65,636
Last modified:November 14, 2003 - v2
Checksum:i716FCC01C59D3F8A
GO

Sequence cautioni

The sequence AAH04762 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AK080419 differs from that shown. Reason: Frameshift at position 46.Curated
The sequence BAA95090 differs from that shown. Reason: Frameshift at position 35.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti470V → A in BAA95090 (Ref. 1) Curated1
Sequence conflicti547K → R in BAA95090 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041607 mRNA. Translation: BAA95090.1. Frameshift.
BC004762 mRNA. Translation: AAH04762.1. Different initiation.
AK080419 mRNA. No translation available.
RefSeqiNP_001297086.1. NM_001310157.1.
UniGeneiMm.116418.

Genome annotation databases

GeneIDi58202.
KEGGimmu:58202.
UCSCiuc008iqm.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiNELFB_MOUSE
AccessioniPrimary (citable) accession number: Q8C4Y3
Secondary accession number(s): Q99J41, Q9JJA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: November 14, 2003
Last modified: November 22, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families