Q8C4Q6 (AIDA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Axin interactor, dorsalization-associated protein

Alternative name(s):
Axin interaction partner and dorsalization antagonist

Gene names

Name:

Aida

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a ventralizing factor during embryogenesis By similarity. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling. Ref.4
Subunit structure	Interacts with AXIN1. Ref.4
Sequence similarities	Belongs to the AIDA family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Molecular function	Developmental protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	determination of ventral identity Inferred from sequence orthology Ref.4. Source: MGI negative regulation of JNK cascade Inferred from mutant phenotype Ref.4. Source: UniProtKB negative regulation of JUN kinase activity Inferred from direct assay Ref.4. Source: MGI negative regulation of determination of dorsal identity Inferred from sequence orthology Ref.4. Source: MGI regulation of protein homodimerization activity Inferred from mutant phenotype Ref.4. Source: UniProtKB
Cellular_component	cytoplasmic part Inferred from direct assay Ref.4. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8C4Q6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8C4Q6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     153-234: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 305

305

Axin interactor, dorsalization-associated protein

PRO_0000305279

Regions

Region

153 – 220

68

Axin-binding

Coiled coil

27 – 62

36

Potential

Natural variations

Alternative sequence

153 – 234

82

Missing in isoform 2.

VSP_028323

Experimental info

Sequence conflict

138

1

G → V in AAH66829. Ref.2

Sequence conflict

171

1

R → K in BAE31624. Ref.1

Sequence conflict

231

1

R → K in BAE34358. Ref.1

Secondary structure

1

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305

Helix Strand Turn

Details...

Helix

4 – 25

22

Helix

28 – 46

19

Beta strand

49 – 51

3

Helix

57 – 78

22

Helix

89 – 92

4

Helix

94 – 99

6

Turn

100 – 103

4

Beta strand

167 – 176

10

Helix

180 – 182

3

Beta strand

184 – 193

10

Beta strand

199 – 201

3

Beta strand

210 – 213

4

Beta strand

216 – 224

9

Helix

229 – 231

3

Beta strand

237 – 246

10

Turn

247 – 250

4

Beta strand

251 – 261

11

Helix

262 – 264

3

Beta strand

267 – 272

6

Beta strand

275 – 278

4

Beta strand

291 – 293

3

Beta strand

296 – 303

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A285C564AF5333D0	FASTA	305	34,888
	10 20 30 40 50 60 MSEVTRSLLQ RWGASLRRGA DFDSWGQLVE AIDEYQILAR HLQKEAQAQH NNSEFTEEQK 70 80 90 100 110 120 KTIGKIATCL ELRSAALQST QSQEEFKLED LKKLEPILKN ILTYNKEFPF DVQPIPLRRI 130 140 150 160 170 180 LAPGEEENLE FEEDEEGGAG AGPPDSFSAR VPGTLLPRLP SEPGMTLLTI RIEKIGLKDA 190 200 210 220 230 240 GQCIDPYITV SVKDLNGIDL TPVQDTPVAS RKEDTYVHFN VDIELQKHVE RLTKGAAIFF 250 260 270 280 290 300 EFKHYKPKKR FTSTKCFAFM EMDEIKPGPI VIELYKKPTD FKRKKLQLLT KKPLYLHLHQ SLHKE
	Isoform 2 [UniParc]. Checksum: F80B8D98D3B0A126	FASTA	223	25,750
	10 20 30 40 50 60 MSEVTRSLLQ RWGASLRRGA DFDSWGQLVE AIDEYQILAR HLQKEAQAQH NNSEFTEEQK 70 80 90 100 110 120 KTIGKIATCL ELRSAALQST QSQEEFKLED LKKLEPILKN ILTYNKEFPF DVQPIPLRRI 130 140 150 160 170 180 LAPGEEENLE FEEDEEGGAG AGPPDSFSAR VPGAAIFFEF KHYKPKKRFT STKCFAFMEM 190 200 210 220 DEIKPGPIVI ELYKKPTDFK RKKLQLLTKK PLYLHLHQSL HKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J and NOD. Tissue: Bone marrow, Embryo, Head, Inner ear, Placenta and Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6 and Czech II. Tissue: Brain, Embryonic germ cell and Mammary tumor.
[3]	"Solution structure of four helical up-and-down bundle domain of the hypothetical protein 2610208m17RIK similar to the protein FLJ12806." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1-115.
[4]	"A beta-catenin-independent dorsalization pathway activated by Axin/JNK signaling and antagonized by aida." Rui Y., Xu Z., Xiong B., Cao Y., Lin S., Zhang M., Chan S.-C., Luo W., Han Y., Lu Z., Ye Z., Zhou H.-M., Han J., Meng A., Lin S.-C. Dev. Cell 13:268-282(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH AXIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK027971 mRNA. Translation: BAC25682.1.
AK081494 mRNA. Translation: BAC38234.1.
AK143716 mRNA. Translation: BAE25512.1.
AK149788 mRNA. Translation: BAE29085.1.
AK150639 mRNA. Translation: BAE29727.1.
AK150702 mRNA. Translation: BAE29781.1.
AK152961 mRNA. Translation: BAE31624.1.
AK153448 mRNA. Translation: BAE32003.1.
AK159318 mRNA. Translation: BAE34985.1.
AK167520 mRNA. Translation: BAE39592.1.
AK172451 mRNA. Translation: BAE43012.1.
AK158103 mRNA. Translation: BAE34358.1.
BC057183 mRNA. Translation: AAH57183.1.
BC066829 mRNA. Translation: AAH66829.1.
BC086763 mRNA. Translation: AAH86763.1.

IPI

IPI00311123.
IPI00664676.

RefSeq

NP_859421.1. NM_181732.4.

UniGene

Mm.290502.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UG7	NMR	-	A	1-115	[»]
2QZ5	X-ray	2.60	A/B	151-305	[»]

ProteinModelPortal

Q8C4Q6.

SMR

Q8C4Q6. Positions 3-115, 151-304.

ModBase

Search...

PTM databases

PhosphoSite

Q8C4Q6.

2D gel databases

REPRODUCTION-2DPAGE

Q8C4Q6.

Proteomic databases

PaxDb

Q8C4Q6.

PRIDE

Q8C4Q6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000109166; ENSMUSP00000104795; ENSMUSG00000042901.

GeneID

108909.

KEGG

mmu:108909.

UCSC

uc008icu.2. mouse.

Organism-specific databases

CTD

64853.

MGI

MGI:1919737. Aida.

Phylogenomic databases

eggNOG

NOG48105.

GeneTree

ENSGT00390000016465.

HOVERGEN

HBG057354.

InParanoid

Q8C4Q6.

OMA

ATCLEMR.

OrthoDB

EOG4640CJ.

Gene expression databases

Bgee

Q8C4Q6.

Genevestigator

Q8C4Q6.

Family and domain databases

Gene3D

1.20.120.360. 1 hit.

InterPro

IPR015006. AIDA.
IPR025939. Aida_C.
IPR023421. AIDA_N.
[Graphical view]

Pfam

PF08910. Aida-C2. 1 hit.
PF14186. Aida_C2. 1 hit.
[Graphical view]

SUPFAM

SSF109779. SSF109779. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

AIDA. mouse.

EvolutionaryTrace

Q8C4Q6.

NextBio

361453.

SOURCE

Search...

Entry information

Entry name

AIDA_MOUSE

Accession

Primary (citable) accession number: Q8C4Q6
Secondary accession number(s): Q3U6V0 , Q3UP85, Q6NXY2, Q6PG77, Q78W09, Q99K80

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program