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Protein

Translation factor Guf1, mitochondrial

Gene

Guf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi57 – 648GTPUniRule annotation
Nucleotide bindingi122 – 1265GTPUniRule annotation
Nucleotide bindingi176 – 1794GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Translation factor Guf1, mitochondrialUniRule annotation (EC:3.6.5.-)
Alternative name(s):
Elongation factor 4 homologUniRule annotation
Short name:
EF-4UniRule annotation
GTPase Guf1UniRule annotation
Ribosomal back-translocaseUniRule annotation
Gene namesi
Name:Guf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2140726. Guf1.

Subcellular locationi

  • Mitochondrion inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Matrix side UniRule annotation

GO - Cellular componenti

  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131MitochondrionUniRule annotationAdd
BLAST
Chaini32 – 651620Translation factor Guf1, mitochondrialPRO_0000256252Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki84 – 84Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)UniRule annotation

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ8C3X4.
MaxQBiQ8C3X4.
PaxDbiQ8C3X4.
PRIDEiQ8C3X4.

PTM databases

iPTMnetiQ8C3X4.
PhosphoSiteiQ8C3X4.

Expressioni

Gene expression databases

BgeeiQ8C3X4.
CleanExiMM_GUF1.
ExpressionAtlasiQ8C3X4. baseline and differential.
GenevisibleiQ8C3X4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084480.

Structurei

3D structure databases

ProteinModelPortaliQ8C3X4.
SMRiQ8C3X4. Positions 48-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 229182tr-type GAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0462. Eukaryota.
COG0481. LUCA.
GeneTreeiENSGT00550000074940.
HOGENOMiHOG000020624.
HOVERGENiHBG081580.
InParanoidiQ8C3X4.
OMAiKPMVFCG.
OrthoDBiEOG7MH0XK.
PhylomeDBiQ8C3X4.
TreeFamiTF314751.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C3X4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWALVGRALA PWAAGARHAA ASEPRAACRL FSAAELKEKP DMSRFPVEDI
60 70 80 90 100
RNFSIIAHVD HGKSTLADRL LELTGTIDKT KKNKQVLDKL QVERERGITV
110 120 130 140 150
KAQTASLFYS FGGKQYLLNL IDTPGHVDFS YEVSRSLSAC QGVLLVVDAN
160 170 180 190 200
EGIQAQTVAN FFLAFEAQLS VIPVINKIDL KNADPERVGK QIEKVFDIPS
210 220 230 240 250
EECIKISAKL GTNVDSVLQA VIERIPPPKV HRENPLKALV FDSTFDQYRG
260 270 280 290 300
VIANIALFDG VVSKGDKIVS AHTKKAYEVN EVGILNPNEQ PTHKLYAGQV
310 320 330 340 350
GFLIAGMKDV TEAQIGDTLY LHNHPVEPLP GFKSAKPMVF AGVYPIDQSE
360 370 380 390 400
YNNLKSAIEK LTLNDSSVTV HRDSSLALGA GWRLGFLGLL HMEVFNQRLE
410 420 430 440 450
QEYNASVILT TPTVPYKAVL SSAKLIKEYK EKEITIINPA QFPEKSQVTE
460 470 480 490 500
YLEPVVLGTV ITPTEYTGKI MALCQARRAI QKNMTFIDEN RVMLKYLFPL
510 520 530 540 550
NEIVVDFYDS LKSLSSGYAS FDYEDAGYQT AELVKMDILL NGNMVEELVT
560 570 580 590 600
VVHREKAYTV GKSICERLKE SLPRQLYEIA IQAAVGSKVI ARETVKAYRK
610 620 630 640 650
NVLAKCYGGD ITRKMKLLKR QSEGKKKLRK IGNIEIPKDA FIKVLKTQPN

K
Length:651
Mass (Da):72,463
Last modified:March 1, 2003 - v1
Checksum:i7DA56A6C43753960
GO
Isoform 2 (identifier: Q8C3X4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     296-383: Missing.

Show »
Length:563
Mass (Da):62,933
Checksum:i75F2DB129305A977
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei296 – 38388Missing in isoform 2. 1 PublicationVSP_021345Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053420 mRNA. Translation: BAC35380.2.
AK084627 mRNA. Translation: BAC39234.1.
BC057962 mRNA. Translation: AAH57962.1.
CCDSiCCDS19322.1. [Q8C3X4-1]
CCDS80296.1. [Q8C3X4-2]
RefSeqiNP_001297560.1. NM_001310631.1. [Q8C3X4-2]
NP_766299.1. NM_172711.3. [Q8C3X4-1]
UniGeneiMm.21105.

Genome annotation databases

EnsembliENSMUST00000031113; ENSMUSP00000031113; ENSMUSG00000029208. [Q8C3X4-2]
ENSMUST00000087228; ENSMUSP00000084480; ENSMUSG00000029208. [Q8C3X4-1]
GeneIDi231279.
KEGGimmu:231279.
UCSCiuc008xqm.1. mouse. [Q8C3X4-1]
uc008xqn.1. mouse. [Q8C3X4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053420 mRNA. Translation: BAC35380.2.
AK084627 mRNA. Translation: BAC39234.1.
BC057962 mRNA. Translation: AAH57962.1.
CCDSiCCDS19322.1. [Q8C3X4-1]
CCDS80296.1. [Q8C3X4-2]
RefSeqiNP_001297560.1. NM_001310631.1. [Q8C3X4-2]
NP_766299.1. NM_172711.3. [Q8C3X4-1]
UniGeneiMm.21105.

3D structure databases

ProteinModelPortaliQ8C3X4.
SMRiQ8C3X4. Positions 48-599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084480.

PTM databases

iPTMnetiQ8C3X4.
PhosphoSiteiQ8C3X4.

Proteomic databases

EPDiQ8C3X4.
MaxQBiQ8C3X4.
PaxDbiQ8C3X4.
PRIDEiQ8C3X4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031113; ENSMUSP00000031113; ENSMUSG00000029208. [Q8C3X4-2]
ENSMUST00000087228; ENSMUSP00000084480; ENSMUSG00000029208. [Q8C3X4-1]
GeneIDi231279.
KEGGimmu:231279.
UCSCiuc008xqm.1. mouse. [Q8C3X4-1]
uc008xqn.1. mouse. [Q8C3X4-2]

Organism-specific databases

CTDi60558.
MGIiMGI:2140726. Guf1.

Phylogenomic databases

eggNOGiKOG0462. Eukaryota.
COG0481. LUCA.
GeneTreeiENSGT00550000074940.
HOGENOMiHOG000020624.
HOVERGENiHBG081580.
InParanoidiQ8C3X4.
OMAiKPMVFCG.
OrthoDBiEOG7MH0XK.
PhylomeDBiQ8C3X4.
TreeFamiTF314751.

Miscellaneous databases

ChiTaRSiGuf1. mouse.
NextBioi380480.
PROiQ8C3X4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C3X4.
CleanExiMM_GUF1.
ExpressionAtlasiQ8C3X4. baseline and differential.
GenevisibleiQ8C3X4. MM.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryonic heart and Eye.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart and Kidney.

Entry informationi

Entry nameiGUF1_MOUSE
AccessioniPrimary (citable) accession number: Q8C3X4
Secondary accession number(s): Q6PEN7, Q8BPS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2003
Last modified: May 11, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.