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Protein

BRCA1-associated ATM activator 1

Gene

Brat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress. Plays a role in regulating mitochondrial function and cell proliferation (By similarity). Required for protein stability of MTOR and MTOR-related proteins, and cell cycle progress by growth factors (PubMed:25657994).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage

Names & Taxonomyi

Protein namesi
Recommended name:
BRCA1-associated ATM activator 1
Alternative name(s):
BRCA1-associated protein required for ATM activation protein 1
Gene namesi
Name:Brat1
Synonyms:Baat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1891679. Brat1.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Present at double strand breaks (DSBs) following ionizing radiation treatment (By similarity). The ubiquitinated form localizes in the nucleus in a NDFIP1-dependent manner.By similarity1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • membrane Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 822822BRCA1-associated ATM activator 1PRO_0000395837Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei743 – 7431PhosphoserineCombined sources

Post-translational modificationi

Ubiquitinated by NEDD4, NEDD4L and ITCH; mono- and polyubiquitinated forms are detected.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8C3R1.
MaxQBiQ8C3R1.
PaxDbiQ8C3R1.
PeptideAtlasiQ8C3R1.
PRIDEiQ8C3R1.

PTM databases

iPTMnetiQ8C3R1.
PhosphoSiteiQ8C3R1.

Expressioni

Tissue specificityi

High levels detected in the cortex and much lower levels detected in the cerebellum, spinal cord and lung (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000000148.
ExpressionAtlasiQ8C3R1. baseline and differential.
GenevisibleiQ8C3R1. MM.

Interactioni

Subunit structurei

Interacts with BRCA1 and ATM. Interacts with MTOR, RPTOR, NDFIP1, SMC1A and PRKDC.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036016.

Structurei

3D structure databases

ProteinModelPortaliQ8C3R1.
SMRiQ8C3R1. Positions 500-657, 690-727.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati495 – 53137HEAT 1Add
BLAST
Repeati544 – 57633HEAT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni100 – 200101Required for interaction with NDFIP1By similarityAdd
BLAST

Sequence similaritiesi

Contains 2 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IEZF. Eukaryota.
ENOG4110J7I. LUCA.
GeneTreeiENSGT00390000017551.
HOVERGENiHBG062139.
InParanoidiQ8C3R1.
PhylomeDBiQ8C3R1.
TreeFamiTF324349.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C3R1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPECSRLLP ALCAVLADPR QLVADDTCLE KLLDWFKTVT EAESSLQLLQ
60 70 80 90 100
DHPCLMELLS HVLKPQDVSP RVLSFALRLV GVFAAQEDCF EYLQQGELLL
110 120 130 140 150
GLFGESGAPG WAAWSIPSVR SGWIQGLCYL AHHPSALHFL ADSGAVDTLF
160 170 180 190 200
SLQGDPSLFV ASAASQLLVH ILALSMQGGA PGSPVPEAAA WPMCAQKIVN
210 220 230 240 250
HVDESLHAKA TPQVTQALNV LTTTFGRCHN PWTGVLWERL SPPVARLFER
260 270 280 290 300
DPIPAVHALM DLLLSVARSP VLNFAACGLW EMLAQTLSRL SPIQAGPLAL
310 320 330 340 350
GTLKLQHCPQ ELRTQAFGVL LQPLACILKA TTQAPGPPGL LDGTVGSLLT
360 370 380 390 400
VDILLASKSA CVGLLCQTLA HLEELQMLPQ CPSPWPQVHL LQAALTILHL
410 420 430 440 450
CDGSADPSSS AGGRLCGTLG GCVRVQRAAL DFLGTLSQGT SPLELVLEVF
460 470 480 490 500
AVLLKTLESP ESSPMVLKKA FQATLRWLQN PHKTPSSSDL SSDALLFLGE
510 520 530 540 550
LFPILQKRLC SPCWEVRDSA LEFLTHLIRH WGGQADFREA LRSSEVPTLA
560 570 580 590 600
LQLLQDPESY VRASAVGAAG QLSSQGLQAA PASPENSQAQ QGLLMDLMHI
610 620 630 640 650
LSTDSEGFPR RAVLRVFTDW LRDGHADVVR DTEWFVATVL QAVSRDLDWE
660 670 680 690 700
VRVQGLELAR VFLTQALGQP SLHCPYTVGL PRASSPRPHP EFLQTLCRLP
710 720 730 740 750
LFEFAFCALL DCDRPVAQKA CDLLLFLRDK TVPCSSPREA GDSPNSASVE
760 770 780 790 800
AALQRWREGE QAQPLGDLDP EAMLAILRAL DLEGLQGRLA KSSDHVEKSP
810 820
QSLLQDMLAT VGVLEENEAD CY
Length:822
Mass (Da):89,086
Last modified:March 1, 2003 - v1
Checksum:i9E0DBE3A5F3E23B9
GO
Isoform 2 (identifier: Q8C3R1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     592-597: GLLMDL → VDTGSW
     598-822: Missing.

Note: No experimental confirmation available.
Show »
Length:597
Mass (Da):64,041
Checksum:iDD0F0226CCB6EAF9
GO

Sequence cautioni

The sequence AAF43013 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE41674 differs from that shown. Reason: Frameshift at positions 133 and 178. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91L → F in AAH68301 (Ref. 2) Curated
Sequence conflicti375 – 3751L → Q in BAE32593 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei592 – 5976GLLMDL → VDTGSW in isoform 2. CuratedVSP_039548
Alternative sequencei598 – 822225Missing in isoform 2. CuratedVSP_039549Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085091 mRNA. Translation: BAC39362.1.
AK170269 mRNA. Translation: BAE41674.1. Frameshift.
AK154448 mRNA. Translation: BAE32593.1.
AK084987 mRNA. Translation: BAC39330.1.
CH466529 Genomic DNA. Translation: EDL19108.1.
BC068301 mRNA. Translation: AAH68301.1.
AF226663 mRNA. Translation: AAF43013.1. Different initiation.
CCDSiCCDS19823.1. [Q8C3R1-1]
RefSeqiNP_001263216.1. NM_001276287.1.
NP_851411.1. NM_181066.3. [Q8C3R1-1]
UniGeneiMm.271932.

Genome annotation databases

EnsembliENSMUST00000041588; ENSMUSP00000036016; ENSMUSG00000000148. [Q8C3R1-1]
ENSMUST00000110806; ENSMUSP00000106429; ENSMUSG00000000148. [Q8C3R1-2]
GeneIDi231841.
KEGGimmu:231841.
UCSCiuc009aib.2. mouse. [Q8C3R1-2]
uc009aic.2. mouse. [Q8C3R1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085091 mRNA. Translation: BAC39362.1.
AK170269 mRNA. Translation: BAE41674.1. Frameshift.
AK154448 mRNA. Translation: BAE32593.1.
AK084987 mRNA. Translation: BAC39330.1.
CH466529 Genomic DNA. Translation: EDL19108.1.
BC068301 mRNA. Translation: AAH68301.1.
AF226663 mRNA. Translation: AAF43013.1. Different initiation.
CCDSiCCDS19823.1. [Q8C3R1-1]
RefSeqiNP_001263216.1. NM_001276287.1.
NP_851411.1. NM_181066.3. [Q8C3R1-1]
UniGeneiMm.271932.

3D structure databases

ProteinModelPortaliQ8C3R1.
SMRiQ8C3R1. Positions 500-657, 690-727.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036016.

PTM databases

iPTMnetiQ8C3R1.
PhosphoSiteiQ8C3R1.

Proteomic databases

EPDiQ8C3R1.
MaxQBiQ8C3R1.
PaxDbiQ8C3R1.
PeptideAtlasiQ8C3R1.
PRIDEiQ8C3R1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041588; ENSMUSP00000036016; ENSMUSG00000000148. [Q8C3R1-1]
ENSMUST00000110806; ENSMUSP00000106429; ENSMUSG00000000148. [Q8C3R1-2]
GeneIDi231841.
KEGGimmu:231841.
UCSCiuc009aib.2. mouse. [Q8C3R1-2]
uc009aic.2. mouse. [Q8C3R1-1]

Organism-specific databases

CTDi221927.
MGIiMGI:1891679. Brat1.

Phylogenomic databases

eggNOGiENOG410IEZF. Eukaryota.
ENOG4110J7I. LUCA.
GeneTreeiENSGT00390000017551.
HOVERGENiHBG062139.
InParanoidiQ8C3R1.
PhylomeDBiQ8C3R1.
TreeFamiTF324349.

Miscellaneous databases

PROiQ8C3R1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000148.
ExpressionAtlasiQ8C3R1. baseline and differential.
GenevisibleiQ8C3R1. MM.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiBRAT1_MOUSE
AccessioniPrimary (citable) accession number: Q8C3R1
Secondary accession number(s): Q3TDC8
, Q3U442, Q6NV65, Q8C3T3, Q9JKU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.