Q8C3P7 (MTA70_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N6-adenosine-methyltransferase 70 kDa subunit
Short name=MT-A70
EC=2.1.1.62

Alternative name(s):
Methyltransferase-like protein 3

Gene names

Name:	Mettl3
Synonyms:	Mta70

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation By similarity.
Catalytic activity	S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.
Subcellular location	Nucleus speckle By similarity. Note: Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components By similarity.
Sequence similarities	Belongs to the MT-A70-like family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB mRNA (N6-adenosine)-methyltransferase activity Inferred from sequence orthology Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8C3P7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8C3P7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     34-106: Missing.
     241-299: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	N6-adenosine-methyltransferase 70 kDa subunit		PRO_0000207631
Amino acid modifications
	Modified residue	43	1	Phosphoserine By similarity
	Modified residue	219	1	Phosphoserine By similarity
	Modified residue	243	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	34 – 106	73	Missing in isoform 2.		VSP_007867
	Alternative sequence	241 – 299	59	Missing in isoform 2.		VSP_007868
Experimental info
	Sequence conflict	162	1	D → E Ref.2
	Sequence conflict	162	1	D → E Ref.3
	Sequence conflict	471	1	R → P in BAB26322. Ref.3
	Sequence conflict	475	1	W → L in BAB26322. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 25, 2003. Version 2. Checksum: 0DBDA2392A37A018	FASTA	580	64,616
	10 20 30 40 50 60 MSDTWSSIQA HKKQLDSLRE RLQRRRKQDS GHLDLRNPEA ALSPTFRSDS PVPTAPTSSG 70 80 90 100 110 120 PKPSTTSVAP ELATDPELEK KLLHHLSDLA LTLPTDAVSI RLAISTPDAP ATQDGVESLL 130 140 150 160 170 180 QKFAAQELIE VKRGLLQDDA HPTLVTYADH SKLSAMMGAV ADKKGLGEVA GTIAGQKRRA 190 200 210 220 230 240 EQDLTTVTTF ASSLASGLAS SASEPAKEPA KKSRKHAASD VDLEIESLLN QQSTKEQQSK 250 260 270 280 290 300 KVSQEILELL NTTTAKEQSI VEKFRSRGRA QVQEFCDYGT KEECMKASDA DRPCRKLHFR 310 320 330 340 350 360 RIINKHTDES LGDCSFLNTC FHMDTCKYVH YEIDACVDSE SPGSKEHMPS QELALTQSVG 370 380 390 400 410 420 GDSSADRLFP PQWICCDIRY LDVSILGKFA VVMADPPWDI HMELPYGTLT DDEMRRLNIP 430 440 450 460 470 480 VLQDDGFLFL WVTGRAMELG RECLNLWGYE RVDEIIWVKT NQLQRIIRTG RTGHWLNHGK 490 500 510 520 530 540 EHCLVGVKGN PQGFNQGLDC DVIVAEVRST SHKPDEIYGM IERLSPGTRK IELFGRPHNV 550 560 570 580 QPNWITLGNQ LDGIHLLDPD VVARFKQRYP DGIISKPKNL
	Isoform 2 [UniParc]. Checksum: 95539DAD61854334	FASTA	448	50,157
	10 20 30 40 50 60 MSDTWSSIQA HKKQLDSLRE RLQRRRKQDS GHLPDAPATQ DGVESLLQKF AAQELIEVKR 70 80 90 100 110 120 GLLQDDAHPT LVTYADHSKL SAMMGAVADK KGLGEVAGTI AGQKRRAEQD LTTVTTFASS 130 140 150 160 170 180 LASGLASSAS EPAKEPAKKS RKHAASDVDL EIESLLNQQS TKEQQSKRRI INKHTDESLG 190 200 210 220 230 240 DCSFLNTCFH MDTCKYVHYE IDACVDSESP GSKEHMPSQE LALTQSVGGD SSADRLFPPQ 250 260 270 280 290 300 WICCDIRYLD VSILGKFAVV MADPPWDIHM ELPYGTLTDD EMRRLNIPVL QDDGFLFLWV 310 320 330 340 350 360 TGRAMELGRE CLNLWGYERV DEIIWVKTNQ LQRIIRTGRT GHWLNHGKEH CLVGVKGNPQ 370 380 390 400 410 420 GFNQGLDCDV IVAEVRSTSH KPDEIYGMIE RLSPGTRKIE LFGRPHNVQP NWITLGNQLD 430 440 GIHLLDPDVV ARFKQRYPDG IISKPKNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase." Bokar J.A., Shambaugh M.E., Polayes D., Matera A.G., Rottman F.M. RNA 3:1233-1247(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1). Strain: 129/Sv.
[2]	"Expression of genes involved in mammalian meiosis during the transition from egg to embryo." Hwang S.-Y., Oh B., Knowles B.B., Solter D., Lee J.-S. Mol. Reprod. Dev. 59:144-158(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 2). Strain: C57BL/6 X DBA/2.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Stomach and Tongue.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF283992 Genomic DNA. Translation: AAG13957.1. AF135789 mRNA. Translation: AAD33673.1. AK009492 mRNA. Translation: BAB26322.1. AK085189 mRNA. Translation: BAC39385.1. BC012526 mRNA. Translation: AAH12526.1.
IPI	IPI00319214. IPI00623291.
RefSeq	NP_062695.2. NM_019721.2.
UniGene	Mm.271759.
3D structure databases
ProteinModelPortal	Q8C3P7.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4102524.
PTM databases
PhosphoSite	Q8C3P7.
Proteomic databases
PaxDb	Q8C3P7.
PRIDE	Q8C3P7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022767; ENSMUSP00000022767; ENSMUSG00000022160.
GeneID	56335.
KEGG	mmu:56335.
UCSC	uc007tpc.1. mouse.
Organism-specific databases
CTD	56339.
MGI	MGI:1927165. Mettl3.
Phylogenomic databases
eggNOG	COG4725.
GeneTree	ENSGT00550000075058.
HOGENOM	HOG000012669.
HOVERGEN	HBG052521.
InParanoid	Q8C3P7.
KO	K05925.
OrthoDB	EOG4JQ3XC.
Gene expression databases
ArrayExpress	Q8C3P7.
Bgee	Q8C3P7.
CleanEx	MM_METTL3.
Genevestigator	Q8C3P7.
GermOnline	ENSMUSG00000022160. Mus musculus.
Family and domain databases
InterPro	IPR025848. MT-A70. IPR007757. MT-A70-like. [Graphical view]
Pfam	PF05063. MT-A70. 1 hit. [Graphical view]
PROSITE	PS51143. MT_A70. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	METTL3. mouse.
NextBio	312322.
SOURCE	Search...

Entry information

Entry name

MTA70_MOUSE

Accession

Primary (citable) accession number: Q8C3P7
Secondary accession number(s): Q9CV54, Q9ERS9, Q9WUI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	July 25, 2003
Last modified:	May 29, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program