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Protein

Layilin

Gene

Layn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for hyaluronate.By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. hyaluronic acid binding Source: HGNC
Complete GO annotation...

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Layilin
Gene namesi
Name:Layn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2685357. Layn.

Subcellular locationi

Membrane Curated; Single-pass type I membrane protein Curated
Note: Colocalizes with TLN1 at the membrane ruffles.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 235211ExtracellularSequence AnalysisAdd
BLAST
Transmembranei236 – 25621HelicalSequence AnalysisAdd
BLAST
Topological domaini257 – 381125CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: HGNC
  2. integral component of membrane Source: UniProtKB-KW
  3. ruffle Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 381357LayilinPRO_0000262509Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi71 ↔ 184PROSITE-ProRule annotation
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi150 ↔ 176PROSITE-ProRule annotation
Modified residuei286 – 2861Phosphoserine1 Publication
Modified residuei299 – 2991Phosphoserine1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8C351.
PRIDEiQ8C351.

PTM databases

PhosphoSiteiQ8C351.

Expressioni

Gene expression databases

BgeeiQ8C351.
CleanExiMM_LAYN.
GenevestigatoriQ8C351.

Interactioni

Subunit structurei

Interacts with TLN1 (By similarity). Interacts with NF2 and RDX.By similarity1 Publication

Structurei

Secondary structure

1
381
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi372 – 3765Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K00NMR-B367-381[»]
ProteinModelPortaliQ8C351.
SMRiQ8C351. Positions 46-179.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8C351.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 185141C-type lectinPROSITE-ProRule annotationAdd
BLAST
Repeati340 – 34451-1
Repeati350 – 35451-2
Repeati356 – 35942-1
Repeati371 – 37551-3
Repeati377 – 38042-2

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni330 – 37445Interaction with NF2Add
BLAST
Regioni337 – 38145Interaction with TLN1By similarityAdd
BLAST
Regioni340 – 375363 X 5 AA repeats of E-S-G-X-VAdd
BLAST
Regioni356 – 380252 X 4 AA repeats of N-X-I-YAdd
BLAST

Domaini

The C-terminal domain interacts with the N-terminal domain of RDX.

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG315174.
GeneTreeiENSGT00390000001844.
HOGENOMiHOG000294068.
HOVERGENiHBG050945.
InParanoidiQ8C351.
KOiK10077.
OMAiTACQDLY.
OrthoDBiEOG7FV3RQ.
TreeFamiTF330715.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C351-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQPGAALQAM LLAVLLAKPR DSKGRLLSAS DLDPRGGQLV CRGGTRRPCY
60 70 80 90 100
KVIYFHDAFQ RLNFEEAKET CMEDGGQLVS IETEDEQRLI EKFIENLLAS
110 120 130 140 150
DGDFWIGLKR LEEKQSNNTA CQDLYAWTDG STSQFRNWYV DEPSCGSEVC
160 170 180 190 200
VVMYHQPSAP PGIGGSYMFQ WNDDRCNMKN NFICKYHDDK PSTTPSIWPG
210 220 230 240 250
GEATEPATPL LPEETQKEDT KETFKERREA ALNLAYILIP SIPLFLLLVV
260 270 280 290 300
TSAVCWVWIC RRKREQTDPS TKEQHTIWPT PRQENSPNLD VYNVIRKQSE
310 320 330 340 350
ADLAEPRPDL KNISFRVCSG EAMPDDMSCD YENIAVNPSE SGFVTLASME
360 370 380
SGFVTNDIYE FSPDRMGRSK ESGWVENEIY Y
Length:381
Mass (Da):43,269
Last modified:July 27, 2011 - v4
Checksum:iCF20C2EE708A5E07
GO
Isoform 2 (identifier: Q8C351-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     136-137: RN → SG
     138-381: Missing.

Show »
Length:137
Mass (Da):15,321
Checksum:i7D76C154D4D17262
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei136 – 1372RN → SG in isoform 2. 1 PublicationVSP_021782
Alternative sequencei138 – 381244Missing in isoform 2. 1 PublicationVSP_021783Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK086930 mRNA. Translation: BAC39765.2.
AK165695 mRNA. Translation: BAE38343.1.
AC119831 Genomic DNA. No translation available.
CCDSiCCDS52791.1. [Q8C351-1]
RefSeqiNP_001028706.1. NM_001033534.1. [Q8C351-1]
UniGeneiMm.335152.

Genome annotation databases

EnsembliENSMUST00000098782; ENSMUSP00000096379; ENSMUSG00000060594. [Q8C351-1]
GeneIDi244864.
KEGGimmu:244864.
UCSCiuc012gtc.1. mouse. [Q8C351-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK086930 mRNA. Translation: BAC39765.2.
AK165695 mRNA. Translation: BAE38343.1.
AC119831 Genomic DNA. No translation available.
CCDSiCCDS52791.1. [Q8C351-1]
RefSeqiNP_001028706.1. NM_001033534.1. [Q8C351-1]
UniGeneiMm.335152.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2K00NMR-B367-381[»]
ProteinModelPortaliQ8C351.
SMRiQ8C351. Positions 46-179.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8C351.

Proteomic databases

MaxQBiQ8C351.
PRIDEiQ8C351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098782; ENSMUSP00000096379; ENSMUSG00000060594. [Q8C351-1]
GeneIDi244864.
KEGGimmu:244864.
UCSCiuc012gtc.1. mouse. [Q8C351-1]

Organism-specific databases

CTDi143903.
MGIiMGI:2685357. Layn.

Phylogenomic databases

eggNOGiNOG315174.
GeneTreeiENSGT00390000001844.
HOGENOMiHOG000294068.
HOVERGENiHBG050945.
InParanoidiQ8C351.
KOiK10077.
OMAiTACQDLY.
OrthoDBiEOG7FV3RQ.
TreeFamiTF330715.

Miscellaneous databases

EvolutionaryTraceiQ8C351.
NextBioi386444.
PROiQ8C351.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C351.
CleanExiMM_LAYN.
GenevestigatoriQ8C351.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-212 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Lung and Medulla oblongata.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Layilin, a cell surface hyaluronan receptor, interacts with merlin and radixin."
    Bono P., Cordero E., Johnson K., Borowsky M., Ramesh V., Jacks T., Hynes R.O.
    Exp. Cell Res. 308:177-187(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NF2 AND RDX.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286 AND SER-299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLAYN_MOUSE
AccessioniPrimary (citable) accession number: Q8C351
Secondary accession number(s): E9QQ23, Q3TMU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 91 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.