Q8C351 (LAYN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Layilin

Gene names

Name:

Layn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for hyaluronate By similarity.
Subunit structure	Interacts with TLN1 By similarity. Interacts with NF2 and RDX. Ref.3
Subcellular location	Membrane; Single-pass type I membrane protein Potential. Note: Colocalizes with TLN1 at the membrane ruffles By similarity.
Domain	The C-terminal domain interacts with the N-terminal domain of RDX.
Sequence similarities	Contains 1 C-type lectin domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Lectin
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cell surface Inferred from sequence or structural similarity. Source: HGNC integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW ruffle Inferred from sequence or structural similarity. Source: HGNC
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro hyaluronic acid binding Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8C351-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8C351-2)

The sequence of this isoform differs from the canonical sequence as follows:
     136-137: RN → SG
     138-381: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Chain

25 – 381

357

Layilin

PRO_0000262509

Regions

Topological domain

25 – 235

211

Extracellular Potential

Transmembrane

236 – 256

21

Helical; Potential

Topological domain

257 – 381

125

Cytoplasmic Potential

Domain

45 – 185

141

C-type lectin

Repeat

340 – 344

5

1-1

Repeat

350 – 354

5

1-2

Repeat

356 – 359

4

2-1

Repeat

371 – 375

5

1-3

Repeat

377 – 380

4

2-2

Region

330 – 374

45

Interaction with NF2

Region

337 – 381

45

Interaction with TLN1 By similarity

Region

340 – 375

36

3 X 5 AA repeats of E-S-G-X-V

Region

356 – 380

25

2 X 4 AA repeats of N-X-I-Y

Amino acid modifications

Modified residue

286

1

Phosphoserine Ref.5

Modified residue

299

1

Phosphoserine Ref.4 Ref.5

Glycosylation

117

1

N-linked (GlcNAc...) Potential

Disulfide bond

71 ↔ 184

By similarity

Disulfide bond

150 ↔ 176

By similarity

Natural variations

Alternative sequence

136 – 137

2

RN → SG in isoform 2.

VSP_021782

Alternative sequence

138 – 381

244

Missing in isoform 2.

VSP_021783

Secondary structure

1

.

.

.

381

Helix Strand Turn

Details...

Beta strand

372 – 376

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 4. Checksum: CF20C2EE708A5E07	FASTA	381	43,269
	10 20 30 40 50 60 MQPGAALQAM LLAVLLAKPR DSKGRLLSAS DLDPRGGQLV CRGGTRRPCY KVIYFHDAFQ 70 80 90 100 110 120 RLNFEEAKET CMEDGGQLVS IETEDEQRLI EKFIENLLAS DGDFWIGLKR LEEKQSNNTA 130 140 150 160 170 180 CQDLYAWTDG STSQFRNWYV DEPSCGSEVC VVMYHQPSAP PGIGGSYMFQ WNDDRCNMKN 190 200 210 220 230 240 NFICKYHDDK PSTTPSIWPG GEATEPATPL LPEETQKEDT KETFKERREA ALNLAYILIP 250 260 270 280 290 300 SIPLFLLLVV TSAVCWVWIC RRKREQTDPS TKEQHTIWPT PRQENSPNLD VYNVIRKQSE 310 320 330 340 350 360 ADLAEPRPDL KNISFRVCSG EAMPDDMSCD YENIAVNPSE SGFVTLASME SGFVTNDIYE 370 380 FSPDRMGRSK ESGWVENEIY Y
	Isoform 2 [UniParc]. Checksum: 7D76C154D4D17262	FASTA	137	15,321
	10 20 30 40 50 60 MQPGAALQAM LLAVLLAKPR DSKGRLLSAS DLDPRGGQLV CRGGTRRPCY KVIYFHDAFQ 70 80 90 100 110 120 RLNFEEAKET CMEDGGQLVS IETEDEQRLI EKFIENLLAS DGDFWIGLKR LEEKQSNNTA 130 CQDLYAWTDG STSQFSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-212 (ISOFORM 1). Strain: C57BL/6J. Tissue: Lung and Medulla oblongata.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Layilin, a cell surface hyaluronan receptor, interacts with merlin and radixin." Bono P., Cordero E., Johnson K., Borowsky M., Ramesh V., Jacks T., Hynes R.O. Exp. Cell Res. 308:177-187(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NF2 AND RDX.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299, MASS SPECTROMETRY. Tissue: Melanoma.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286 AND SER-299, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK086930 mRNA. Translation: BAC39765.2.
AK165695 mRNA. Translation: BAE38343.1.
AC119831 Genomic DNA. No translation available.

IPI

IPI00352139.
IPI00987592.

RefSeq

NP_001028706.1. NM_001033534.1.

UniGene

Mm.335152.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2K00	NMR	-	B	367-381	[»]

ProteinModelPortal

Q8C351.

SMR

Q8C351. Positions 46-179.

ModBase

Search...

PTM databases

PhosphoSite

Q8C351.

Proteomic databases

PRIDE

Q8C351.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000098782; ENSMUSP00000096379; ENSMUSG00000060594.

GeneID

244864.

KEGG

mmu:244864.

Organism-specific databases

CTD

143903.

MGI

MGI:2685357. Layn.

Phylogenomic databases

eggNOG

NOG315174.

GeneTree

ENSGT00390000001844.

HOGENOM

HOG000294068.

HOVERGEN

HBG050945.

InParanoid

Q8C351.

KO

K10077.

OMA

AKEACRR.

OrthoDB

EOG4FN4JN.

Gene expression databases

Bgee

Q8C351.

CleanEx

MM_LAYN.

Genevestigator

Q8C351.

GermOnline

ENSMUSG00000060594. Mus musculus.

Family and domain databases

Gene3D

3.10.100.10. 1 hit.

InterPro

IPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]

Pfam

PF00059. Lectin_C. 1 hit.
[Graphical view]

SMART

SM00034. CLECT. 1 hit.
[Graphical view]

SUPFAM

SSF56436. C-type_lectin_fold. 1 hit.

PROSITE

PS00615. C_TYPE_LECTIN_1. False negative.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8C351.

NextBio

386444.

SOURCE

Search...

Entry information

Entry name

LAYN_MOUSE

Accession

Primary (citable) accession number: Q8C351
Secondary accession number(s): E9QQ23, Q3TMU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 77 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program