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Protein

F-box/LRR-repeat protein 5

Gene

Fbxl5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Iron 1By similarity1
Metal bindingi57Iron 1By similarity1
Metal bindingi58Iron 1By similarity1
Metal bindingi61Iron 1By similarity1
Metal bindingi61Iron 2By similarity1
Metal bindingi80Iron 2By similarity1
Metal bindingi126Iron 2By similarity1
Metal bindingi130Iron 1By similarity1
Metal bindingi130Iron 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/LRR-repeat protein 5
Alternative name(s):
F-box and leucine-rich repeat protein 5
Gene namesi
Name:Fbxl5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2152883. Fbxl5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001198461 – 690F-box/LRR-repeat protein 5Add BLAST690

Post-translational modificationi

Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8C2S5.
PRIDEiQ8C2S5.

PTM databases

PhosphoSitePlusiQ8C2S5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039753.
CleanExiMM_FBXL5.
ExpressionAtlasiQ8C2S5. baseline and differential.
GenevisibleiQ8C2S5. MM.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with ACO1, IREB2/IRP2; the interaction depends on the 4Fe-4S cluster. Interacts with DCTN1/p150-glued (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045792.

Structurei

3D structure databases

ProteinModelPortaliQ8C2S5.
SMRiQ8C2S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini202 – 248F-boxPROSITE-ProRule annotationAdd BLAST47
Repeati340 – 364LRR 1Add BLAST25
Repeati365 – 392LRR 2Add BLAST28
Repeati393 – 418LRR 3Add BLAST26
Repeati478 – 507LRR 4Add BLAST30
Repeati575 – 606LRR 5Add BLAST32
Repeati607 – 634LRR 6Add BLAST28
Repeati635 – 660LRR 7Add BLAST26

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 159Hemerythrin-likeBy similarityAdd BLAST159

Domaini

The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded (By similarity).By similarity

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 7 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IHDI. Eukaryota.
ENOG410ZKEM. LUCA.
GeneTreeiENSGT00390000006172.
HOVERGENiHBG051589.
InParanoidiQ8C2S5.
KOiK10271.
OMAiTKQYACL.
OrthoDBiEOG091G02J5.
PhylomeDBiQ8C2S5.
TreeFamiTF331105.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR001810. F-box_dom.
IPR012312. Haemerythrin-like.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF01814. Hemerythrin. 1 hit.
PF13516. LRR_6. 2 hits.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 4 hits.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C2S5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPFPDEVDV FTAPHWRMKQ LVGRYCDKLS KTNFSNNNDF RALLQSLYAT
60 70 80 90 100
FKEFKMHEQI ENEYIIGLLQ QRSQTIYNVH SDNKLSEMLS LFEKGLKNVK
110 120 130 140 150
NEYEQLNYAK QLKERLEAFT RDFLPHMKEE EEVFQPMLME YFTYEELKDI
160 170 180 190 200
KKKVIAQHCS QKDTAELLRG LSLWNQAEER QKVLKYSVDE KADTEAEVSE
210 220 230 240 250
HSTGITHLPP EVMLSIFSYL NPQELCRCSQ VSTKWSQLAK TGSLWKHLYP
260 270 280 290 300
VHWARGDWYS GPATELDTEP DEEWVRNRKD ESRAFQEWDE DADIDESEES
310 320 330 340 350
AEESVAISIA QMEKRVLHGL IHNVLPYVGT SVKTLVLAYS SAVSSKMVRQ
360 370 380 390 400
ILELCPNLEH LDLTQTDISD SAFDSWSWLG CCQSLRHLDL SGCEKITDMA
410 420 430 440 450
LEKISRALGV LTSHQSGFLK SAGKAASTPW TSKDITMPST TQYACLHNLT
460 470 480 490 500
DKGIGEEIDN EHSWTEPVSS ESLTSPYVWM LDAEDLADIE DAVEWRHRNV
510 520 530 540 550
ESLCVMETAS NFGCSSSGCY SKDIVGLRTS VCWQQHCASP AFAYCGHSFC
560 570 580 590 600
CTGTALRTMT TLPATSAMCR KALRTTLPRG KDLIYFGSEK SDQETGRVLL
610 620 630 640 650
FLSLSGCYQI TDHGLRALTL GGGLPYLEHL NLSGCLTVTG AGLQDLVSAC
660 670 680 690
PSLNDEYFYY CDNINGPHAD TASGCQNLQC GFRACCRSGE
Length:690
Mass (Da):77,894
Last modified:October 3, 2003 - v2
Checksum:i9558476AFB3ECCA0
GO
Isoform 2 (identifier: Q8C2S5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MAPFPDEVDVFTAPHWRMKQLVGRYCDK → MRNNQELKYEIKWKHLDCSVVE

Note: No experimental confirmation available.
Show »
Length:684
Mass (Da):77,319
Checksum:iB1B47D533E3FC8DA
GO
Isoform 3 (identifier: Q8C2S5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     617-623: ALTLGGG → WVISPSC
     624-690: Missing.

Show »
Length:623
Mass (Da):70,924
Checksum:i9DAE11360271A8AE
GO
Isoform 4 (identifier: Q8C2S5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     298-298: Missing.

Note: No experimental confirmation available.
Show »
Length:689
Mass (Da):77,765
Checksum:i47DD6E9530CD54F7
GO
Isoform 5 (identifier: Q8C2S5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     29-71: Missing.
     617-623: ALTLGGG → WVISPSC
     624-690: Missing.

Note: No experimental confirmation available.
Show »
Length:580
Mass (Da):65,804
Checksum:iB024C4DE375A3E7C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105Q → R in BAC38698 (PubMed:16141072).Curated1
Sequence conflicti127M → K in BAC40126 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0084181 – 28MAPFP…RYCDK → MRNNQELKYEIKWKHLDCSV VE in isoform 2. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_03852929 – 71Missing in isoform 5. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_038530298Missing in isoform 4. 1 Publication1
Alternative sequenceiVSP_008419617 – 623ALTLGGG → WVISPSC in isoform 3 and isoform 5. 2 Publications7
Alternative sequenceiVSP_008420624 – 690Missing in isoform 3 and isoform 5. 2 PublicationsAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029957 mRNA. Translation: BAC26698.1.
AK041497 mRNA. Translation: BAC30964.1.
AK082931 mRNA. Translation: BAC38698.1.
AK085100 mRNA. Translation: BAC39366.1.
AK088067 mRNA. Translation: BAC40126.1.
AK150664 mRNA. Translation: BAE29748.1.
AK159744 mRNA. Translation: BAE35337.1.
AK171783 mRNA. Translation: BAE42662.1.
BC047214 mRNA. Translation: AAH47214.1.
BC113798 mRNA. Translation: AAI13799.1.
CCDSiCCDS19263.1. [Q8C2S5-3]
CCDS51489.1. [Q8C2S5-1]
RefSeqiNP_001153435.1. NM_001159963.1. [Q8C2S5-1]
NP_848844.1. NM_178729.4. [Q8C2S5-3]
UniGeneiMm.25794.

Genome annotation databases

EnsembliENSMUST00000047857; ENSMUSP00000045792; ENSMUSG00000039753. [Q8C2S5-1]
ENSMUST00000087465; ENSMUSP00000084733; ENSMUSG00000039753. [Q8C2S5-3]
ENSMUST00000114047; ENSMUSP00000109681; ENSMUSG00000039753. [Q8C2S5-2]
ENSMUST00000121736; ENSMUSP00000112444; ENSMUSG00000039753. [Q8C2S5-5]
ENSMUST00000196483; ENSMUSP00000143703; ENSMUSG00000039753. [Q8C2S5-4]
GeneIDi242960.
KEGGimmu:242960.
UCSCiuc008xhu.2. mouse. [Q8C2S5-1]
uc008xhv.2. mouse. [Q8C2S5-4]
uc008xhw.2. mouse. [Q8C2S5-3]
uc008xhx.2. mouse. [Q8C2S5-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029957 mRNA. Translation: BAC26698.1.
AK041497 mRNA. Translation: BAC30964.1.
AK082931 mRNA. Translation: BAC38698.1.
AK085100 mRNA. Translation: BAC39366.1.
AK088067 mRNA. Translation: BAC40126.1.
AK150664 mRNA. Translation: BAE29748.1.
AK159744 mRNA. Translation: BAE35337.1.
AK171783 mRNA. Translation: BAE42662.1.
BC047214 mRNA. Translation: AAH47214.1.
BC113798 mRNA. Translation: AAI13799.1.
CCDSiCCDS19263.1. [Q8C2S5-3]
CCDS51489.1. [Q8C2S5-1]
RefSeqiNP_001153435.1. NM_001159963.1. [Q8C2S5-1]
NP_848844.1. NM_178729.4. [Q8C2S5-3]
UniGeneiMm.25794.

3D structure databases

ProteinModelPortaliQ8C2S5.
SMRiQ8C2S5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045792.

PTM databases

PhosphoSitePlusiQ8C2S5.

Proteomic databases

PaxDbiQ8C2S5.
PRIDEiQ8C2S5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047857; ENSMUSP00000045792; ENSMUSG00000039753. [Q8C2S5-1]
ENSMUST00000087465; ENSMUSP00000084733; ENSMUSG00000039753. [Q8C2S5-3]
ENSMUST00000114047; ENSMUSP00000109681; ENSMUSG00000039753. [Q8C2S5-2]
ENSMUST00000121736; ENSMUSP00000112444; ENSMUSG00000039753. [Q8C2S5-5]
ENSMUST00000196483; ENSMUSP00000143703; ENSMUSG00000039753. [Q8C2S5-4]
GeneIDi242960.
KEGGimmu:242960.
UCSCiuc008xhu.2. mouse. [Q8C2S5-1]
uc008xhv.2. mouse. [Q8C2S5-4]
uc008xhw.2. mouse. [Q8C2S5-3]
uc008xhx.2. mouse. [Q8C2S5-5]

Organism-specific databases

CTDi26234.
MGIiMGI:2152883. Fbxl5.

Phylogenomic databases

eggNOGiENOG410IHDI. Eukaryota.
ENOG410ZKEM. LUCA.
GeneTreeiENSGT00390000006172.
HOVERGENiHBG051589.
InParanoidiQ8C2S5.
KOiK10271.
OMAiTKQYACL.
OrthoDBiEOG091G02J5.
PhylomeDBiQ8C2S5.
TreeFamiTF331105.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiFbxl5. mouse.
PROiQ8C2S5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039753.
CleanExiMM_FBXL5.
ExpressionAtlasiQ8C2S5. baseline and differential.
GenevisibleiQ8C2S5. MM.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR001810. F-box_dom.
IPR012312. Haemerythrin-like.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR006553. Leu-rich_rpt_Cys-con_subtyp.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF01814. Hemerythrin. 1 hit.
PF13516. LRR_6. 2 hits.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
SM00367. LRR_CC. 4 hits.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBXL5_MOUSE
AccessioniPrimary (citable) accession number: Q8C2S5
Secondary accession number(s): Q14CG1
, Q3TAK6, Q3TWC9, Q3UC66, Q80XI5, Q8BGF5, Q8BNL3, Q8C3Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.