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Q8C2S5 (FBXL5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
F-box/LRR-repeat protein 5
Alternative name(s):
F-box and leucine-rich repeat protein 5
Gene names
Name:Fbxl5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length690 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with ACO1, IREB2/IRP2; the interaction depends on the 4Fe-4S cluster. Interacts with DCTN1/p150-glued By similarity.

Subcellular location

Cytoplasmperinuclear region By similarity.

Domain

The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded By similarity.

Post-translational modification

Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor By similarity.

Sequence similarities

Contains 1 F-box domain.

Contains 7 LRR (leucine-rich) repeats.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8C2S5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8C2S5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MAPFPDEVDVFTAPHWRMKQLVGRYCDK → MRNNQELKYEIKWKHLDCSVVE
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8C2S5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     617-623: ALTLGGG → WVISPSC
     624-690: Missing.
Isoform 4 (identifier: Q8C2S5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     298-298: Missing.
Note: No experimental confirmation available.
Isoform 5 (identifier: Q8C2S5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     29-71: Missing.
     617-623: ALTLGGG → WVISPSC
     624-690: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 690690F-box/LRR-repeat protein 5
PRO_0000119846

Regions

Domain202 – 24847F-box
Repeat340 – 36425LRR 1
Repeat365 – 39228LRR 2
Repeat393 – 41826LRR 3
Repeat478 – 50730LRR 4
Repeat575 – 60632LRR 5
Repeat607 – 63428LRR 6
Repeat635 – 66026LRR 7
Region1 – 159159Hemerythrin-like By similarity

Sites

Metal binding151Iron 1 By similarity
Metal binding571Iron 1 By similarity
Metal binding581Iron 1 By similarity
Metal binding611Iron 1 By similarity
Metal binding611Iron 2 By similarity
Metal binding801Iron 2 By similarity
Metal binding1261Iron 2 By similarity
Metal binding1301Iron 1 By similarity
Metal binding1301Iron 2 By similarity

Natural variations

Alternative sequence1 – 2828MAPFP…RYCDK → MRNNQELKYEIKWKHLDCSV VE in isoform 2.
VSP_008418
Alternative sequence29 – 7143Missing in isoform 5.
VSP_038529
Alternative sequence2981Missing in isoform 4.
VSP_038530
Alternative sequence617 – 6237ALTLGGG → WVISPSC in isoform 3 and isoform 5.
VSP_008419
Alternative sequence624 – 69067Missing in isoform 3 and isoform 5.
VSP_008420

Experimental info

Sequence conflict1051Q → R in BAC38698. Ref.1
Sequence conflict1271M → K in BAC40126. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 3, 2003. Version 2.
Checksum: 9558476AFB3ECCA0

FASTA69077,894
        10         20         30         40         50         60 
MAPFPDEVDV FTAPHWRMKQ LVGRYCDKLS KTNFSNNNDF RALLQSLYAT FKEFKMHEQI 

        70         80         90        100        110        120 
ENEYIIGLLQ QRSQTIYNVH SDNKLSEMLS LFEKGLKNVK NEYEQLNYAK QLKERLEAFT 

       130        140        150        160        170        180 
RDFLPHMKEE EEVFQPMLME YFTYEELKDI KKKVIAQHCS QKDTAELLRG LSLWNQAEER 

       190        200        210        220        230        240 
QKVLKYSVDE KADTEAEVSE HSTGITHLPP EVMLSIFSYL NPQELCRCSQ VSTKWSQLAK 

       250        260        270        280        290        300 
TGSLWKHLYP VHWARGDWYS GPATELDTEP DEEWVRNRKD ESRAFQEWDE DADIDESEES 

       310        320        330        340        350        360 
AEESVAISIA QMEKRVLHGL IHNVLPYVGT SVKTLVLAYS SAVSSKMVRQ ILELCPNLEH 

       370        380        390        400        410        420 
LDLTQTDISD SAFDSWSWLG CCQSLRHLDL SGCEKITDMA LEKISRALGV LTSHQSGFLK 

       430        440        450        460        470        480 
SAGKAASTPW TSKDITMPST TQYACLHNLT DKGIGEEIDN EHSWTEPVSS ESLTSPYVWM 

       490        500        510        520        530        540 
LDAEDLADIE DAVEWRHRNV ESLCVMETAS NFGCSSSGCY SKDIVGLRTS VCWQQHCASP 

       550        560        570        580        590        600 
AFAYCGHSFC CTGTALRTMT TLPATSAMCR KALRTTLPRG KDLIYFGSEK SDQETGRVLL 

       610        620        630        640        650        660 
FLSLSGCYQI TDHGLRALTL GGGLPYLEHL NLSGCLTVTG AGLQDLVSAC PSLNDEYFYY 

       670        680        690 
CDNINGPHAD TASGCQNLQC GFRACCRSGE 

« Hide

Isoform 2 [UniParc].

Checksum: B1B47D533E3FC8DA
Show »

FASTA68477,319
Isoform 3 [UniParc].

Checksum: 9DAE11360271A8AE
Show »

FASTA62370,924
Isoform 4 [UniParc].

Checksum: 47DD6E9530CD54F7
Show »

FASTA68977,765
Isoform 5 [UniParc].

Checksum: B024C4DE375A3E7C
Show »

FASTA58065,804

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3; 4 AND 5).
Strain: C57BL/6J and NOD.
Tissue: Bone marrow, Lung, Spleen, Testis and Thymus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Eye.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK029957 mRNA. Translation: BAC26698.1.
AK041497 mRNA. Translation: BAC30964.1.
AK082931 mRNA. Translation: BAC38698.1.
AK085100 mRNA. Translation: BAC39366.1.
AK088067 mRNA. Translation: BAC40126.1.
AK150664 mRNA. Translation: BAE29748.1.
AK159744 mRNA. Translation: BAE35337.1.
AK171783 mRNA. Translation: BAE42662.1.
BC047214 mRNA. Translation: AAH47214.1.
BC113798 mRNA. Translation: AAI13799.1.
RefSeqNP_001153435.1. NM_001159963.1.
NP_848844.1. NM_178729.4.
UniGeneMm.25794.

3D structure databases

ProteinModelPortalQ8C2S5.
SMRQ8C2S5. Positions 4-160, 205-245, 304-419, 596-656.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000045792.

PTM databases

PhosphoSiteQ8C2S5.

Proteomic databases

PRIDEQ8C2S5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047857; ENSMUSP00000045792; ENSMUSG00000039753. [Q8C2S5-1]
ENSMUST00000087465; ENSMUSP00000084733; ENSMUSG00000039753. [Q8C2S5-3]
ENSMUST00000114047; ENSMUSP00000109681; ENSMUSG00000039753. [Q8C2S5-2]
ENSMUST00000121736; ENSMUSP00000112444; ENSMUSG00000039753. [Q8C2S5-5]
GeneID242960.
KEGGmmu:242960.
UCSCuc008xhu.2. mouse. [Q8C2S5-1]
uc008xhv.2. mouse. [Q8C2S5-4]
uc008xhw.2. mouse. [Q8C2S5-3]
uc008xhx.2. mouse. [Q8C2S5-5]

Organism-specific databases

CTD26234.
MGIMGI:2152883. Fbxl5.

Phylogenomic databases

eggNOGNOG29620.
GeneTreeENSGT00390000006172.
HOVERGENHBG051589.
InParanoidQ3UC66.
KOK10271.
OMATKQYACL.
OrthoDBEOG76MK7S.
PhylomeDBQ8C2S5.
TreeFamTF331105.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ8C2S5.
BgeeQ8C2S5.
CleanExMM_FBXL5.
GenevestigatorQ8C2S5.

Family and domain databases

InterProIPR001810. F-box_dom.
IPR012312. Haemerythrin/HHE_cat-bd_motif.
[Graphical view]
PfamPF01814. Hemerythrin. 1 hit.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMSSF81383. SSF81383. 1 hit.
PROSITEPS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFBXL5. mouse.
NextBio385622.
PROQ8C2S5.
SOURCESearch...

Entry information

Entry nameFBXL5_MOUSE
AccessionPrimary (citable) accession number: Q8C2S5
Secondary accession number(s): Q14CG1 expand/collapse secondary AC list , Q3TAK6, Q3TWC9, Q3UC66, Q80XI5, Q8BGF5, Q8BNL3, Q8C3Q8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot