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Q8C2S5

- FBXL5_MOUSE

UniProt

Q8C2S5 - FBXL5_MOUSE

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Protein

F-box/LRR-repeat protein 5

Gene

Fbxl5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi15 – 151Iron 1By similarity
Metal bindingi57 – 571Iron 1By similarity
Metal bindingi58 – 581Iron 1By similarity
Metal bindingi61 – 611Iron 1By similarity
Metal bindingi61 – 611Iron 2By similarity
Metal bindingi80 – 801Iron 2By similarity
Metal bindingi126 – 1261Iron 2By similarity
Metal bindingi130 – 1301Iron 1By similarity
Metal bindingi130 – 1301Iron 2By similarity

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB

GO - Biological processi

  1. iron ion homeostasis Source: UniProtKB
  2. protein ubiquitination Source: UniProtKB
  3. SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_244970. Association of TriC/CCT with target proteins during biosynthesis.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
F-box/LRR-repeat protein 5
Alternative name(s):
F-box and leucine-rich repeat protein 5
Gene namesi
Name:Fbxl5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:2152883. Fbxl5.

Subcellular locationi

Cytoplasmperinuclear region By similarity

GO - Cellular componenti

  1. perinuclear region of cytoplasm Source: UniProtKB
  2. SCF ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690F-box/LRR-repeat protein 5PRO_0000119846Add
BLAST

Post-translational modificationi

Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ8C2S5.
PRIDEiQ8C2S5.

PTM databases

PhosphoSiteiQ8C2S5.

Expressioni

Gene expression databases

BgeeiQ8C2S5.
CleanExiMM_FBXL5.
ExpressionAtlasiQ8C2S5. baseline and differential.
GenevestigatoriQ8C2S5.

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with ACO1, IREB2/IRP2; the interaction depends on the 4Fe-4S cluster. Interacts with DCTN1/p150-glued (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045792.

Structurei

3D structure databases

ProteinModelPortaliQ8C2S5.
SMRiQ8C2S5. Positions 4-160, 304-419, 597-663.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 24847F-boxPROSITE-ProRule annotationAdd
BLAST
Repeati340 – 36425LRR 1Add
BLAST
Repeati365 – 39228LRR 2Add
BLAST
Repeati393 – 41826LRR 3Add
BLAST
Repeati478 – 50730LRR 4Add
BLAST
Repeati575 – 60632LRR 5Add
BLAST
Repeati607 – 63428LRR 6Add
BLAST
Repeati635 – 66026LRR 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 159159Hemerythrin-likeBy similarityAdd
BLAST

Domaini

The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded (By similarity).By similarity

Sequence similaritiesi

Contains 1 F-box domain.PROSITE-ProRule annotation
Contains 7 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiNOG29620.
GeneTreeiENSGT00390000006172.
HOVERGENiHBG051589.
InParanoidiQ8C2S5.
KOiK10271.
OMAiTKQYACL.
OrthoDBiEOG76MK7S.
PhylomeDBiQ8C2S5.
TreeFamiTF331105.

Family and domain databases

InterProiIPR001810. F-box_dom.
IPR012312. Haemerythrin/HHE_cat-bd_motif.
[Graphical view]
PfamiPF12937. F-box-like. 1 hit.
PF01814. Hemerythrin. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMiSSF81383. SSF81383. 1 hit.
PROSITEiPS50181. FBOX. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8C2S5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPFPDEVDV FTAPHWRMKQ LVGRYCDKLS KTNFSNNNDF RALLQSLYAT
60 70 80 90 100
FKEFKMHEQI ENEYIIGLLQ QRSQTIYNVH SDNKLSEMLS LFEKGLKNVK
110 120 130 140 150
NEYEQLNYAK QLKERLEAFT RDFLPHMKEE EEVFQPMLME YFTYEELKDI
160 170 180 190 200
KKKVIAQHCS QKDTAELLRG LSLWNQAEER QKVLKYSVDE KADTEAEVSE
210 220 230 240 250
HSTGITHLPP EVMLSIFSYL NPQELCRCSQ VSTKWSQLAK TGSLWKHLYP
260 270 280 290 300
VHWARGDWYS GPATELDTEP DEEWVRNRKD ESRAFQEWDE DADIDESEES
310 320 330 340 350
AEESVAISIA QMEKRVLHGL IHNVLPYVGT SVKTLVLAYS SAVSSKMVRQ
360 370 380 390 400
ILELCPNLEH LDLTQTDISD SAFDSWSWLG CCQSLRHLDL SGCEKITDMA
410 420 430 440 450
LEKISRALGV LTSHQSGFLK SAGKAASTPW TSKDITMPST TQYACLHNLT
460 470 480 490 500
DKGIGEEIDN EHSWTEPVSS ESLTSPYVWM LDAEDLADIE DAVEWRHRNV
510 520 530 540 550
ESLCVMETAS NFGCSSSGCY SKDIVGLRTS VCWQQHCASP AFAYCGHSFC
560 570 580 590 600
CTGTALRTMT TLPATSAMCR KALRTTLPRG KDLIYFGSEK SDQETGRVLL
610 620 630 640 650
FLSLSGCYQI TDHGLRALTL GGGLPYLEHL NLSGCLTVTG AGLQDLVSAC
660 670 680 690
PSLNDEYFYY CDNINGPHAD TASGCQNLQC GFRACCRSGE
Length:690
Mass (Da):77,894
Last modified:October 3, 2003 - v2
Checksum:i9558476AFB3ECCA0
GO
Isoform 2 (identifier: Q8C2S5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: MAPFPDEVDVFTAPHWRMKQLVGRYCDK → MRNNQELKYEIKWKHLDCSVVE

Note: No experimental confirmation available.

Show »
Length:684
Mass (Da):77,319
Checksum:iB1B47D533E3FC8DA
GO
Isoform 3 (identifier: Q8C2S5-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     617-623: ALTLGGG → WVISPSC
     624-690: Missing.

Show »
Length:623
Mass (Da):70,924
Checksum:i9DAE11360271A8AE
GO
Isoform 4 (identifier: Q8C2S5-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     298-298: Missing.

Note: No experimental confirmation available.

Show »
Length:689
Mass (Da):77,765
Checksum:i47DD6E9530CD54F7
GO
Isoform 5 (identifier: Q8C2S5-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     29-71: Missing.
     617-623: ALTLGGG → WVISPSC
     624-690: Missing.

Note: No experimental confirmation available.

Show »
Length:580
Mass (Da):65,804
Checksum:iB024C4DE375A3E7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051Q → R in BAC38698. (PubMed:16141072)Curated
Sequence conflicti127 – 1271M → K in BAC40126. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2828MAPFP…RYCDK → MRNNQELKYEIKWKHLDCSV VE in isoform 2. 1 PublicationVSP_008418Add
BLAST
Alternative sequencei29 – 7143Missing in isoform 5. 1 PublicationVSP_038529Add
BLAST
Alternative sequencei298 – 2981Missing in isoform 4. 1 PublicationVSP_038530
Alternative sequencei617 – 6237ALTLGGG → WVISPSC in isoform 3 and isoform 5. 2 PublicationsVSP_008419
Alternative sequencei624 – 69067Missing in isoform 3 and isoform 5. 2 PublicationsVSP_008420Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029957 mRNA. Translation: BAC26698.1.
AK041497 mRNA. Translation: BAC30964.1.
AK082931 mRNA. Translation: BAC38698.1.
AK085100 mRNA. Translation: BAC39366.1.
AK088067 mRNA. Translation: BAC40126.1.
AK150664 mRNA. Translation: BAE29748.1.
AK159744 mRNA. Translation: BAE35337.1.
AK171783 mRNA. Translation: BAE42662.1.
BC047214 mRNA. Translation: AAH47214.1.
BC113798 mRNA. Translation: AAI13799.1.
CCDSiCCDS19263.1. [Q8C2S5-3]
CCDS51489.1. [Q8C2S5-1]
RefSeqiNP_001153435.1. NM_001159963.1. [Q8C2S5-1]
NP_848844.1. NM_178729.4. [Q8C2S5-3]
UniGeneiMm.25794.

Genome annotation databases

EnsembliENSMUST00000047857; ENSMUSP00000045792; ENSMUSG00000039753. [Q8C2S5-1]
ENSMUST00000087465; ENSMUSP00000084733; ENSMUSG00000039753. [Q8C2S5-3]
ENSMUST00000114047; ENSMUSP00000109681; ENSMUSG00000039753. [Q8C2S5-2]
ENSMUST00000121736; ENSMUSP00000112444; ENSMUSG00000039753. [Q8C2S5-5]
GeneIDi242960.
KEGGimmu:242960.
UCSCiuc008xhu.2. mouse. [Q8C2S5-1]
uc008xhv.2. mouse. [Q8C2S5-4]
uc008xhw.2. mouse. [Q8C2S5-3]
uc008xhx.2. mouse. [Q8C2S5-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029957 mRNA. Translation: BAC26698.1 .
AK041497 mRNA. Translation: BAC30964.1 .
AK082931 mRNA. Translation: BAC38698.1 .
AK085100 mRNA. Translation: BAC39366.1 .
AK088067 mRNA. Translation: BAC40126.1 .
AK150664 mRNA. Translation: BAE29748.1 .
AK159744 mRNA. Translation: BAE35337.1 .
AK171783 mRNA. Translation: BAE42662.1 .
BC047214 mRNA. Translation: AAH47214.1 .
BC113798 mRNA. Translation: AAI13799.1 .
CCDSi CCDS19263.1. [Q8C2S5-3 ]
CCDS51489.1. [Q8C2S5-1 ]
RefSeqi NP_001153435.1. NM_001159963.1. [Q8C2S5-1 ]
NP_848844.1. NM_178729.4. [Q8C2S5-3 ]
UniGenei Mm.25794.

3D structure databases

ProteinModelPortali Q8C2S5.
SMRi Q8C2S5. Positions 4-160, 304-419, 597-663.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000045792.

PTM databases

PhosphoSitei Q8C2S5.

Proteomic databases

MaxQBi Q8C2S5.
PRIDEi Q8C2S5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047857 ; ENSMUSP00000045792 ; ENSMUSG00000039753 . [Q8C2S5-1 ]
ENSMUST00000087465 ; ENSMUSP00000084733 ; ENSMUSG00000039753 . [Q8C2S5-3 ]
ENSMUST00000114047 ; ENSMUSP00000109681 ; ENSMUSG00000039753 . [Q8C2S5-2 ]
ENSMUST00000121736 ; ENSMUSP00000112444 ; ENSMUSG00000039753 . [Q8C2S5-5 ]
GeneIDi 242960.
KEGGi mmu:242960.
UCSCi uc008xhu.2. mouse. [Q8C2S5-1 ]
uc008xhv.2. mouse. [Q8C2S5-4 ]
uc008xhw.2. mouse. [Q8C2S5-3 ]
uc008xhx.2. mouse. [Q8C2S5-5 ]

Organism-specific databases

CTDi 26234.
MGIi MGI:2152883. Fbxl5.

Phylogenomic databases

eggNOGi NOG29620.
GeneTreei ENSGT00390000006172.
HOVERGENi HBG051589.
InParanoidi Q8C2S5.
KOi K10271.
OMAi TKQYACL.
OrthoDBi EOG76MK7S.
PhylomeDBi Q8C2S5.
TreeFami TF331105.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_244970. Association of TriC/CCT with target proteins during biosynthesis.

Miscellaneous databases

ChiTaRSi Fbxl5. mouse.
NextBioi 385622.
PROi Q8C2S5.
SOURCEi Search...

Gene expression databases

Bgeei Q8C2S5.
CleanExi MM_FBXL5.
ExpressionAtlasi Q8C2S5. baseline and differential.
Genevestigatori Q8C2S5.

Family and domain databases

InterProi IPR001810. F-box_dom.
IPR012312. Haemerythrin/HHE_cat-bd_motif.
[Graphical view ]
Pfami PF12937. F-box-like. 1 hit.
PF01814. Hemerythrin. 1 hit.
[Graphical view ]
SMARTi SM00256. FBOX. 1 hit.
[Graphical view ]
SUPFAMi SSF81383. SSF81383. 1 hit.
PROSITEi PS50181. FBOX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3; 4 AND 5).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Lung, Spleen, Testis and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Eye.

Entry informationi

Entry nameiFBXL5_MOUSE
AccessioniPrimary (citable) accession number: Q8C2S5
Secondary accession number(s): Q14CG1
, Q3TAK6, Q3TWC9, Q3UC66, Q80XI5, Q8BGF5, Q8BNL3, Q8C3Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: November 26, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3