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Q8C2S5

- FBXL5_MOUSE

UniProt

Q8C2S5 - FBXL5_MOUSE

Protein

F-box/LRR-repeat protein 5

Gene

Fbxl5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (03 Oct 2003)
      Previous versions | rss
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    Functioni

    Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi15 – 151Iron 1By similarity
    Metal bindingi57 – 571Iron 1By similarity
    Metal bindingi58 – 581Iron 1By similarity
    Metal bindingi61 – 611Iron 1By similarity
    Metal bindingi61 – 611Iron 2By similarity
    Metal bindingi80 – 801Iron 2By similarity
    Metal bindingi126 – 1261Iron 2By similarity
    Metal bindingi130 – 1301Iron 1By similarity
    Metal bindingi130 – 1301Iron 2By similarity

    GO - Molecular functioni

    1. iron ion binding Source: UniProtKB

    GO - Biological processi

    1. iron ion homeostasis Source: UniProtKB
    2. protein ubiquitination Source: UniProtKB
    3. SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    F-box/LRR-repeat protein 5
    Alternative name(s):
    F-box and leucine-rich repeat protein 5
    Gene namesi
    Name:Fbxl5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:2152883. Fbxl5.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity

    GO - Cellular componenti

    1. perinuclear region of cytoplasm Source: UniProtKB
    2. SCF ubiquitin ligase complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 690690F-box/LRR-repeat protein 5PRO_0000119846Add
    BLAST

    Post-translational modificationi

    Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor By similarity.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiQ8C2S5.

    PTM databases

    PhosphoSiteiQ8C2S5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8C2S5.
    BgeeiQ8C2S5.
    CleanExiMM_FBXL5.
    GenevestigatoriQ8C2S5.

    Interactioni

    Subunit structurei

    Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with ACO1, IREB2/IRP2; the interaction depends on the 4Fe-4S cluster. Interacts with DCTN1/p150-glued By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000045792.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8C2S5.
    SMRiQ8C2S5. Positions 4-160, 329-408, 596-678.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini202 – 24847F-boxPROSITE-ProRule annotationAdd
    BLAST
    Repeati340 – 36425LRR 1Add
    BLAST
    Repeati365 – 39228LRR 2Add
    BLAST
    Repeati393 – 41826LRR 3Add
    BLAST
    Repeati478 – 50730LRR 4Add
    BLAST
    Repeati575 – 60632LRR 5Add
    BLAST
    Repeati607 – 63428LRR 6Add
    BLAST
    Repeati635 – 66026LRR 7Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 159159Hemerythrin-likeBy similarityAdd
    BLAST

    Domaini

    The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded By similarity.By similarity

    Sequence similaritiesi

    Contains 1 F-box domain.PROSITE-ProRule annotation
    Contains 7 LRR (leucine-rich) repeats.Curated

    Keywords - Domaini

    Leucine-rich repeat, Repeat

    Phylogenomic databases

    eggNOGiNOG29620.
    GeneTreeiENSGT00390000006172.
    HOVERGENiHBG051589.
    InParanoidiQ3UC66.
    KOiK10271.
    OMAiTKQYACL.
    OrthoDBiEOG76MK7S.
    PhylomeDBiQ8C2S5.
    TreeFamiTF331105.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    IPR012312. Haemerythrin/HHE_cat-bd_motif.
    [Graphical view]
    PfamiPF12937. F-box-like. 1 hit.
    PF01814. Hemerythrin. 1 hit.
    [Graphical view]
    SMARTiSM00256. FBOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF81383. SSF81383. 1 hit.
    PROSITEiPS50181. FBOX. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8C2S5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAPFPDEVDV FTAPHWRMKQ LVGRYCDKLS KTNFSNNNDF RALLQSLYAT    50
    FKEFKMHEQI ENEYIIGLLQ QRSQTIYNVH SDNKLSEMLS LFEKGLKNVK 100
    NEYEQLNYAK QLKERLEAFT RDFLPHMKEE EEVFQPMLME YFTYEELKDI 150
    KKKVIAQHCS QKDTAELLRG LSLWNQAEER QKVLKYSVDE KADTEAEVSE 200
    HSTGITHLPP EVMLSIFSYL NPQELCRCSQ VSTKWSQLAK TGSLWKHLYP 250
    VHWARGDWYS GPATELDTEP DEEWVRNRKD ESRAFQEWDE DADIDESEES 300
    AEESVAISIA QMEKRVLHGL IHNVLPYVGT SVKTLVLAYS SAVSSKMVRQ 350
    ILELCPNLEH LDLTQTDISD SAFDSWSWLG CCQSLRHLDL SGCEKITDMA 400
    LEKISRALGV LTSHQSGFLK SAGKAASTPW TSKDITMPST TQYACLHNLT 450
    DKGIGEEIDN EHSWTEPVSS ESLTSPYVWM LDAEDLADIE DAVEWRHRNV 500
    ESLCVMETAS NFGCSSSGCY SKDIVGLRTS VCWQQHCASP AFAYCGHSFC 550
    CTGTALRTMT TLPATSAMCR KALRTTLPRG KDLIYFGSEK SDQETGRVLL 600
    FLSLSGCYQI TDHGLRALTL GGGLPYLEHL NLSGCLTVTG AGLQDLVSAC 650
    PSLNDEYFYY CDNINGPHAD TASGCQNLQC GFRACCRSGE 690
    Length:690
    Mass (Da):77,894
    Last modified:October 3, 2003 - v2
    Checksum:i9558476AFB3ECCA0
    GO
    Isoform 2 (identifier: Q8C2S5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-28: MAPFPDEVDVFTAPHWRMKQLVGRYCDK → MRNNQELKYEIKWKHLDCSVVE

    Note: No experimental confirmation available.

    Show »
    Length:684
    Mass (Da):77,319
    Checksum:iB1B47D533E3FC8DA
    GO
    Isoform 3 (identifier: Q8C2S5-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         617-623: ALTLGGG → WVISPSC
         624-690: Missing.

    Show »
    Length:623
    Mass (Da):70,924
    Checksum:i9DAE11360271A8AE
    GO
    Isoform 4 (identifier: Q8C2S5-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         298-298: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:689
    Mass (Da):77,765
    Checksum:i47DD6E9530CD54F7
    GO
    Isoform 5 (identifier: Q8C2S5-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         29-71: Missing.
         617-623: ALTLGGG → WVISPSC
         624-690: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:580
    Mass (Da):65,804
    Checksum:iB024C4DE375A3E7C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti105 – 1051Q → R in BAC38698. (PubMed:16141072)Curated
    Sequence conflicti127 – 1271M → K in BAC40126. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2828MAPFP…RYCDK → MRNNQELKYEIKWKHLDCSV VE in isoform 2. 1 PublicationVSP_008418Add
    BLAST
    Alternative sequencei29 – 7143Missing in isoform 5. 1 PublicationVSP_038529Add
    BLAST
    Alternative sequencei298 – 2981Missing in isoform 4. 1 PublicationVSP_038530
    Alternative sequencei617 – 6237ALTLGGG → WVISPSC in isoform 3 and isoform 5. 2 PublicationsVSP_008419
    Alternative sequencei624 – 69067Missing in isoform 3 and isoform 5. 2 PublicationsVSP_008420Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK029957 mRNA. Translation: BAC26698.1.
    AK041497 mRNA. Translation: BAC30964.1.
    AK082931 mRNA. Translation: BAC38698.1.
    AK085100 mRNA. Translation: BAC39366.1.
    AK088067 mRNA. Translation: BAC40126.1.
    AK150664 mRNA. Translation: BAE29748.1.
    AK159744 mRNA. Translation: BAE35337.1.
    AK171783 mRNA. Translation: BAE42662.1.
    BC047214 mRNA. Translation: AAH47214.1.
    BC113798 mRNA. Translation: AAI13799.1.
    CCDSiCCDS19263.1. [Q8C2S5-3]
    CCDS51489.1. [Q8C2S5-1]
    RefSeqiNP_001153435.1. NM_001159963.1. [Q8C2S5-1]
    NP_848844.1. NM_178729.4. [Q8C2S5-3]
    UniGeneiMm.25794.

    Genome annotation databases

    EnsembliENSMUST00000047857; ENSMUSP00000045792; ENSMUSG00000039753. [Q8C2S5-1]
    ENSMUST00000087465; ENSMUSP00000084733; ENSMUSG00000039753. [Q8C2S5-3]
    ENSMUST00000114047; ENSMUSP00000109681; ENSMUSG00000039753. [Q8C2S5-2]
    ENSMUST00000121736; ENSMUSP00000112444; ENSMUSG00000039753. [Q8C2S5-5]
    GeneIDi242960.
    KEGGimmu:242960.
    UCSCiuc008xhu.2. mouse. [Q8C2S5-1]
    uc008xhv.2. mouse. [Q8C2S5-4]
    uc008xhw.2. mouse. [Q8C2S5-3]
    uc008xhx.2. mouse. [Q8C2S5-5]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK029957 mRNA. Translation: BAC26698.1 .
    AK041497 mRNA. Translation: BAC30964.1 .
    AK082931 mRNA. Translation: BAC38698.1 .
    AK085100 mRNA. Translation: BAC39366.1 .
    AK088067 mRNA. Translation: BAC40126.1 .
    AK150664 mRNA. Translation: BAE29748.1 .
    AK159744 mRNA. Translation: BAE35337.1 .
    AK171783 mRNA. Translation: BAE42662.1 .
    BC047214 mRNA. Translation: AAH47214.1 .
    BC113798 mRNA. Translation: AAI13799.1 .
    CCDSi CCDS19263.1. [Q8C2S5-3 ]
    CCDS51489.1. [Q8C2S5-1 ]
    RefSeqi NP_001153435.1. NM_001159963.1. [Q8C2S5-1 ]
    NP_848844.1. NM_178729.4. [Q8C2S5-3 ]
    UniGenei Mm.25794.

    3D structure databases

    ProteinModelPortali Q8C2S5.
    SMRi Q8C2S5. Positions 4-160, 329-408, 596-678.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000045792.

    PTM databases

    PhosphoSitei Q8C2S5.

    Proteomic databases

    PRIDEi Q8C2S5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047857 ; ENSMUSP00000045792 ; ENSMUSG00000039753 . [Q8C2S5-1 ]
    ENSMUST00000087465 ; ENSMUSP00000084733 ; ENSMUSG00000039753 . [Q8C2S5-3 ]
    ENSMUST00000114047 ; ENSMUSP00000109681 ; ENSMUSG00000039753 . [Q8C2S5-2 ]
    ENSMUST00000121736 ; ENSMUSP00000112444 ; ENSMUSG00000039753 . [Q8C2S5-5 ]
    GeneIDi 242960.
    KEGGi mmu:242960.
    UCSCi uc008xhu.2. mouse. [Q8C2S5-1 ]
    uc008xhv.2. mouse. [Q8C2S5-4 ]
    uc008xhw.2. mouse. [Q8C2S5-3 ]
    uc008xhx.2. mouse. [Q8C2S5-5 ]

    Organism-specific databases

    CTDi 26234.
    MGIi MGI:2152883. Fbxl5.

    Phylogenomic databases

    eggNOGi NOG29620.
    GeneTreei ENSGT00390000006172.
    HOVERGENi HBG051589.
    InParanoidi Q3UC66.
    KOi K10271.
    OMAi TKQYACL.
    OrthoDBi EOG76MK7S.
    PhylomeDBi Q8C2S5.
    TreeFami TF331105.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi FBXL5. mouse.
    NextBioi 385622.
    PROi Q8C2S5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8C2S5.
    Bgeei Q8C2S5.
    CleanExi MM_FBXL5.
    Genevestigatori Q8C2S5.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    IPR012312. Haemerythrin/HHE_cat-bd_motif.
    [Graphical view ]
    Pfami PF12937. F-box-like. 1 hit.
    PF01814. Hemerythrin. 1 hit.
    [Graphical view ]
    SMARTi SM00256. FBOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81383. SSF81383. 1 hit.
    PROSITEi PS50181. FBOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3; 4 AND 5).
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Lung, Spleen, Testis and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Tissue: Eye.

    Entry informationi

    Entry nameiFBXL5_MOUSE
    AccessioniPrimary (citable) accession number: Q8C2S5
    Secondary accession number(s): Q14CG1
    , Q3TAK6, Q3TWC9, Q3UC66, Q80XI5, Q8BGF5, Q8BNL3, Q8C3Q8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: October 3, 2003
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3