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Q8C2S0 (UBP44_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 44

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 44
Ubiquitin thioesterase 44
Ubiquitin-specific-processing protease 44
Gene names
Name:Usp44
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination. Ref.1

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Nucleus. Note: Peaks in interphase, with relatively low levels maintained throughout mitosis. Ref.1

Tissue specificity

Widely expressed. Highly expressed in lung, pancreas, skin, liver, stomach and intestine. Ref.5

Post-translational modification

Dephosphorylated by CTDP1 By similarity.

Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome. Ref.1

Sequence similarities

Belongs to the peptidase C19 family. USP44 subfamily.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence caution

The sequence AAI11887.1 differs from that shown. Reason: Intron retention.

The sequence BAC40148.1 differs from that shown. Reason: Intron retention.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711Ubiquitin carboxyl-terminal hydrolase 44
PRO_0000395811

Regions

Domain272 – 677406USP
Zinc finger24 – 8562UBP-type

Sites

Active site2811Nucleophile By similarity
Active site6351Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8C2S0 [UniParc].

Last modified November 28, 2012. Version 3.
Checksum: F176DB2BA3C658BE

FASTA71180,479
        10         20         30         40         50         60 
MDRCKHVEQL QLAQGHSILD PQKWYCMVCN TTESIWACLS CSHVACGKYI QEHALKHFQE 

        70         80         90        100        110        120 
SSHPVAFEVN DMYAFCYLCN DYVLNDNAAG DLKSLRSTLS TIKSKKYPCV VPSDSVLHPV 

       130        140        150        160        170        180 
DAQDRVYSLL DGTQSLPGNE DPTCAALWHR RRVLMGKAFR TWFEQSAIGR KGQEPTQERM 

       190        200        210        220        230        240 
VAKREAKRRQ QQELEQQMKA ELESTPPRKS LRLQGSSEEA ATIEIVPVRA PPPPPASPAK 

       250        260        270        280        290        300 
DKAALPTSED RTFKKVSDSL IKRRPMVTPG VTGLRNLGNT CYMNSVLQVL SHLLIFRQCF 

       310        320        330        340        350        360 
LKLDLNQWLA VAASDKARSY KHSAVTEAAA QQMNEGQEKE KGFVCSRHSG LSSGLSGGAS 

       370        380        390        400        410        420 
KGRNMELIQP REPSSPYSSL CHELHILFQV MWSGEWALVS PFAMLHSVWR LIPAFRGYAQ 

       430        440        450        460        470        480 
QDAQEFLCEL LDKIQRELET TGTKLPALIP TSQRRLIEQV LNVVNNIFHG QFLSQVTCLA 

       490        500        510        520        530        540 
CDNKSDTIES FWDLSLEFPE RYQCSGKDAA SQPCLVTDML DKFTETEALE GKIYMCDHCN 

       550        560        570        580        590        600 
SKRRKFSSKS VVFTEAQKQL MICHLPQVLR LHLKRFRWSG RNNREKIGVH VVFEETLNME 

       610        620        630        640        650        660 
PYCCRETLNA LRPECFLYNL SAVVIHHGKG FGSGHYTAYC YNSEGGFWVH CNDSKLSMCT 

       670        680        690        700        710 
MEEVRKAQAY ILFYTQRVTE NGHSKLLPPE LLSNSQHPSK ETDASSNEVL S 

« Hide

References

« Hide 'large scale' references
[1]"Overexpression of ubiquitin specific protease 44 (USP44) induces chromosomal instability and is frequently observed in human T-cell leukemia."
Zhang Y., van Deursen J., Galardy P.J.
PLoS ONE 6:E23389-E23389(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, PROTEASOMAL DEGRADATION.
Tissue: Kidney.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
Tissue: Thymus.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"K48- and K63-linked polyubiquitination of deubiquitinating enzyme USP44."
Suresh B., Ramakrishna S., Lee H.J., Choi J.H., Kim J.Y., Ahn W.S., Baek K.H.
Cell Biol. Int. 34:799-808(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK088109 mRNA. Translation: BAC40148.1. Sequence problems.
CJ236846 mRNA. No translation available.
AC124686 Genomic DNA. No translation available.
BC111886 mRNA. Translation: AAI11887.1. Sequence problems.
RefSeqNP_001193780.1. NM_001206851.1.
UniGeneMm.491598.

3D structure databases

ProteinModelPortalQ8C2S0.
SMRQ8C2S0. Positions 26-83, 268-677.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid236490. 1 interaction.
STRING10090.ENSMUSP00000092975.

PTM databases

PhosphoSiteQ8C2S0.

Proteomic databases

PRIDEQ8C2S0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID327799.
KEGGmmu:327799.
UCSCuc011xlw.1. mouse.

Organism-specific databases

CTD84101.
MGIMGI:3045318. Usp44.

Phylogenomic databases

eggNOGKOG1867.
HOGENOMHOG000015084.
HOVERGENHBG018027.
InParanoidQ8C2S0.
KOK11834.
OrthoDBEOG7JX33S.

Gene expression databases

BgeeQ8C2S0.
GenevestigatorQ8C2S0.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio397964.
PROQ8C2S0.
SOURCESearch...

Entry information

Entry nameUBP44_MOUSE
AccessionPrimary (citable) accession number: Q8C2S0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: November 28, 2012
Last modified: April 16, 2014
This is version 92 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot