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Protein

Ubiquitin carboxyl-terminal hydrolase 44

Gene

Usp44

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei281NucleophilePROSITE-ProRule annotation1
Active sitei635Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri24 – 85UBP-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: CACAO
  • negative regulation of ubiquitin protein ligase activity Source: MGI
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein deubiquitination Source: MGI
  • regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.057

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 44 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 44
Ubiquitin thioesterase 44
Ubiquitin-specific-processing protease 44
Gene namesi
Name:Usp44
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:3045318 Usp44

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003958111 – 711Ubiquitin carboxyl-terminal hydrolase 44Add BLAST711

Post-translational modificationi

Dephosphorylated by CTDP1.By similarity
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination and is degraded by the proteasome.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ8C2S0

PTM databases

iPTMnetiQ8C2S0
PhosphoSitePlusiQ8C2S0

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in lung, pancreas, skin, liver, stomach and intestine.1 Publication

Gene expression databases

ExpressionAtlasiQ8C2S0 baseline and differential

Interactioni

Protein-protein interaction databases

BioGridi236490, 2 interactors

Structurei

3D structure databases

ProteinModelPortaliQ8C2S0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini272 – 677USPAdd BLAST406

Sequence similaritiesi

Belongs to the peptidase C19 family. USP44 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri24 – 85UBP-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00890000139343
HOGENOMiHOG000015084
HOVERGENiHBG018027
InParanoidiQ8C2S0
KOiK11834

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR018200 USP_CS
IPR028889 USP_dom
IPR013083 Znf_RING/FYVE/PHD
IPR001607 Znf_UBP
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PF02148 zf-UBP, 1 hit
SMARTiView protein in SMART
SM00290 ZnF_UBP, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit
PS50271 ZF_UBP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8C2S0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRCKHVEQL QLAQGHSILD PQKWYCMVCN TTESIWACLS CSHVACGKYI
60 70 80 90 100
QEHALKHFQE SSHPVAFEVN DMYAFCYLCN DYVLNDNAAG DLKSLRSTLS
110 120 130 140 150
TIKSKKYPCV VPSDSVLHPV DAQDRVYSLL DGTQSLPGNE DPTCAALWHR
160 170 180 190 200
RRVLMGKAFR TWFEQSAIGR KGQEPTQERM VAKREAKRRQ QQELEQQMKA
210 220 230 240 250
ELESTPPRKS LRLQGSSEEA ATIEIVPVRA PPPPPASPAK DKAALPTSED
260 270 280 290 300
RTFKKVSDSL IKRRPMVTPG VTGLRNLGNT CYMNSVLQVL SHLLIFRQCF
310 320 330 340 350
LKLDLNQWLA VAASDKARSY KHSAVTEAAA QQMNEGQEKE KGFVCSRHSG
360 370 380 390 400
LSSGLSGGAS KGRNMELIQP REPSSPYSSL CHELHILFQV MWSGEWALVS
410 420 430 440 450
PFAMLHSVWR LIPAFRGYAQ QDAQEFLCEL LDKIQRELET TGTKLPALIP
460 470 480 490 500
TSQRRLIEQV LNVVNNIFHG QFLSQVTCLA CDNKSDTIES FWDLSLEFPE
510 520 530 540 550
RYQCSGKDAA SQPCLVTDML DKFTETEALE GKIYMCDHCN SKRRKFSSKS
560 570 580 590 600
VVFTEAQKQL MICHLPQVLR LHLKRFRWSG RNNREKIGVH VVFEETLNME
610 620 630 640 650
PYCCRETLNA LRPECFLYNL SAVVIHHGKG FGSGHYTAYC YNSEGGFWVH
660 670 680 690 700
CNDSKLSMCT MEEVRKAQAY ILFYTQRVTE NGHSKLLPPE LLSNSQHPSK
710
ETDASSNEVL S
Length:711
Mass (Da):80,479
Last modified:November 28, 2012 - v3
Checksum:iF176DB2BA3C658BE
GO

Sequence cautioni

The sequence AAI11887 differs from that shown. Intron retention.Curated
The sequence BAC40148 differs from that shown. Intron retention.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK088109 mRNA Translation: BAC40148.1 Sequence problems.
CJ236846 mRNA No translation available.
AC124686 Genomic DNA No translation available.
BC111886 mRNA Translation: AAI11887.1 Sequence problems.
RefSeqiNP_001193780.1, NM_001206851.1
XP_011241781.1, XM_011243479.2
XP_011241782.1, XM_011243480.2
XP_011241783.1, XM_011243481.2
XP_011241784.1, XM_011243482.2
XP_011241785.1, XM_011243483.2
XP_011241786.1, XM_011243484.2
XP_011241787.1, XM_011243485.2
XP_011241788.1, XM_011243486.2
XP_011241789.1, XM_011243487.2
XP_011241790.1, XM_011243488.2
XP_011241791.1, XM_011243489.2
XP_011241792.1, XM_011243490.2
UniGeneiMm.491598

Genome annotation databases

EnsembliENSMUST00000216224; ENSMUSP00000149020; ENSMUSG00000020020
GeneIDi327799
KEGGimmu:327799
UCSCiuc029rep.1 mouse

Similar proteinsi

Entry informationi

Entry nameiUBP44_MOUSE
AccessioniPrimary (citable) accession number: Q8C2S0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: November 28, 2012
Last modified: February 28, 2018
This is version 119 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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