Q8C2K5 (RASL3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RAS protein activator like-3

Gene names

Name:

Rasal3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1041 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as a Ras GTPase-activating protein By similarity.
Sequence similarities	Contains 1 C2 domain. Contains 1 PH domain. Contains 1 Ras-GAP domain.
Caution	It is uncertain whether Met-1 or Met-23 is the initiator.
Sequence caution	The sequence BAC30956.1 differs from that shown. Reason: Frameshift at position 11.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of Ras protein signal transduction Inferred from Biological aspect of Ancestor. Source: RefGenome signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome intrinsic to internal side of plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	Ras GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8C2K5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8C2K5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.
     132-132: E → ERSKQAMVPGVKGQLSGVSSLLQLQ

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1041	1041	RAS protein activator like-3		PRO_0000322567
Regions
	Domain	220 – 321	102	PH
	Domain	334 – 410	77	C2
	Domain	484 – 676	193	Ras-GAP
	Coiled coil	218 – 243	26	Potential
	Coiled coil	931 – 1013	83	Potential
Amino acid modifications
	Modified residue	41	1	Phosphoserine By similarity
	Modified residue	187	1	Phosphoserine By similarity
	Modified residue	190	1	Phosphoserine By similarity
	Modified residue	259	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 22	22	Missing in isoform 2.		VSP_031931
	Alternative sequence	132	1	E → ERSKQAMVPGVKGQLSGVSS LLQLQ in isoform 2.		VSP_031932
Experimental info
	Sequence conflict	122	1	L → M in BAC27141. Ref.1
	Sequence conflict	494	1	R → H in BAC27141. Ref.1
	Sequence conflict	978	1	G → S in BAC40689. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: DA58139782B1EEFF	FASTA	1,041	114,782
	10 20 30 40 50 60 MKPECGQTMF RTFWSRSRDS SAMDPPLQSE EDSQTQPSLP SPLTSYRWHT GGSGEKAAGG 70 80 90 100 110 120 FRWGRFAGWG RALSHQEPMV NSQPAPRSLF RRVLSAPPKE SRSNRLRFSK TLWGRHKNVA 130 140 150 160 170 180 PLEPKPNPKA PEPELELVAD PDLPVAQIPE PPTPDMPVWN IDGFTLLEGK LVMLGEEEGP 190 200 210 220 230 240 RQIRVGSASS ENSMQAALGN LKDAVRTPGK TEPEAAGSNQ VHNVRKLLKR LKEKKRAKSE 250 260 270 280 290 300 LGAYTPRDGP PSALGSRESL ATLSELDLGA ERDVRVWPLH PSLLGEPYCF QVTWAGGSLC 310 320 330 340 350 360 FSCRSSAERD RWIEDLRRQF QPSQDNVERQ EMWLTVWVHE AKGLPRATVP GVRAELWLDG 370 380 390 400 410 420 ALLARTAPRA GPGQLFWAER FHFEALPPAR RLSLRLRSAG PAGATVGRVV LELDEVSIPR 430 440 450 460 470 480 APAAGLERWF PVLGAPAGAV LRARIRVRCL RVLPSERYKE LAEFLTFHYA RLCGALEPAL 490 500 510 520 530 540 SAQAKEELAA AMVRVLRATG RAQALVTDLG TAELARCGGR EALLFRENTL ATKAIDEYMK 550 560 570 580 590 600 LVAQEYLQDT LGQVVRCLCA STEDCEVDPS KCPTPELPKH QARLRDSCEE VFENIIHSYN 610 620 630 640 650 660 CFPAELGSVF SSWREACKAR GSEALGPRLV CASLFLRLLC PAILAPSLFG LAPEHPAPGP 670 680 690 700 710 720 ARTLTLIAKV IQNLANCAPF GEKEAYMAFM NSFLEDHGPA MQHFLDQVAT VDADTTPSGY 730 740 750 760 770 780 QGSGDLALQL AVLHVQLCTI FAELDQKTQD SLEPLPTILR AIEEGRPVPV SVPMRLPRIS 790 800 810 820 830 840 TQVQSSFFSG EKPGFLAPRD LPKHTPLISK SQSLRSFQGA GSWASRRPDE ERPQRRPRPV 850 860 870 880 890 900 LRTQSVPARR PTHRRPSAGS KPRPKGSLRM GPAPCGRAWT RASASLPRKP SVPWQRQMDQ 910 920 930 940 950 960 PGDRYQTTGT HRPVGKLAEI QCEVAIFREA QKALSLLVES LSTQVQALKE QQEHFRCQLQ 970 980 990 1000 1010 1020 DLYSRLGAGI SKLDSKGGLP SNGSHRLKSL EQRLTEMECS QDQLRDSLQS LQLLSKTPGS 1030 1040 RSQPLPLKAP CVNGADLSMG T
	Isoform 2 [UniParc]. Checksum: BB7FACC5B3C42A49	FASTA	1,043	114,685
	10 20 30 40 50 60 MDPPLQSEED SQTQPSLPSP LTSYRWHTGG SGEKAAGGFR WGRFAGWGRA LSHQEPMVNS 70 80 90 100 110 120 QPAPRSLFRR VLSAPPKESR SNRLRFSKTL WGRHKNVAPL EPKPNPKAPE RSKQAMVPGV 130 140 150 160 170 180 KGQLSGVSSL LQLQPELELV ADPDLPVAQI PEPPTPDMPV WNIDGFTLLE GKLVMLGEEE 190 200 210 220 230 240 GPRQIRVGSA SSENSMQAAL GNLKDAVRTP GKTEPEAAGS NQVHNVRKLL KRLKEKKRAK 250 260 270 280 290 300 SELGAYTPRD GPPSALGSRE SLATLSELDL GAERDVRVWP LHPSLLGEPY CFQVTWAGGS 310 320 330 340 350 360 LCFSCRSSAE RDRWIEDLRR QFQPSQDNVE RQEMWLTVWV HEAKGLPRAT VPGVRAELWL 370 380 390 400 410 420 DGALLARTAP RAGPGQLFWA ERFHFEALPP ARRLSLRLRS AGPAGATVGR VVLELDEVSI 430 440 450 460 470 480 PRAPAAGLER WFPVLGAPAG AVLRARIRVR CLRVLPSERY KELAEFLTFH YARLCGALEP 490 500 510 520 530 540 ALSAQAKEEL AAAMVRVLRA TGRAQALVTD LGTAELARCG GREALLFREN TLATKAIDEY 550 560 570 580 590 600 MKLVAQEYLQ DTLGQVVRCL CASTEDCEVD PSKCPTPELP KHQARLRDSC EEVFENIIHS 610 620 630 640 650 660 YNCFPAELGS VFSSWREACK ARGSEALGPR LVCASLFLRL LCPAILAPSL FGLAPEHPAP 670 680 690 700 710 720 GPARTLTLIA KVIQNLANCA PFGEKEAYMA FMNSFLEDHG PAMQHFLDQV ATVDADTTPS 730 740 750 760 770 780 GYQGSGDLAL QLAVLHVQLC TIFAELDQKT QDSLEPLPTI LRAIEEGRPV PVSVPMRLPR 790 800 810 820 830 840 ISTQVQSSFF SGEKPGFLAP RDLPKHTPLI SKSQSLRSFQ GAGSWASRRP DEERPQRRPR 850 860 870 880 890 900 PVLRTQSVPA RRPTHRRPSA GSKPRPKGSL RMGPAPCGRA WTRASASLPR KPSVPWQRQM 910 920 930 940 950 960 DQPGDRYQTT GTHRPVGKLA EIQCEVAIFR EAQKALSLLV ESLSTQVQAL KEQQEHFRCQ 970 980 990 1000 1010 1020 LQDLYSRLGA GISKLDSKGG LPSNGSHRLK SLEQRLTEME CSQDQLRDSL QSLQLLSKTP 1030 1040 GSRSQPLPLK APCVNGADLS MGT

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J and NOD. Tissue: Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK030797 mRNA. Translation: BAC27141.1. AK041479 mRNA. Translation: BAC30956.1. Frameshift. AK088449 mRNA. Translation: BAC40358.1. AK088987 mRNA. Translation: BAC40689.1. BC132341 mRNA. Translation: AAI32342.2.
IPI	IPI00624211. IPI00844774.
RefSeq	NP_848900.2. NM_178785.3.
UniGene	Mm.122284. Mm.416246.
3D structure databases
ProteinModelPortal	Q8C2K5.
SMR	Q8C2K5. Positions 444-762.
ModBase	Search...
PTM databases
PhosphoSite	Q8C2K5.
Proteomic databases
PaxDb	Q8C2K5.
PRIDE	Q8C2K5.
Protocols and materials databases
DNASU	320484.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000063824; ENSMUSP00000064084; ENSMUSG00000052142. ENSMUST00000136375; ENSMUSP00000118738; ENSMUSG00000052142. ENSMUST00000137458; ENSMUSP00000123141; ENSMUSG00000052142.
GeneID	320484.
KEGG	mmu:320484.
UCSC	uc008bwu.1. mouse. uc008bwv.1. mouse.
Organism-specific databases
CTD	64926.
MGI	MGI:2444128. Rasal3.
Phylogenomic databases
eggNOG	NOG313834.
GeneTree	ENSGT00600000084217.
HOGENOM	HOG000154074.
InParanoid	Q8C2K5.
OMA	LPSERYK.
OrthoDB	EOG4SF958.
Gene expression databases
ArrayExpress	Q8C2K5.
Bgee	Q8C2K5.
CleanEx	MM_A430107D22RIK.
Genevestigator	Q8C2K5.
Family and domain databases
Gene3D	1.10.506.10. 1 hit.
InterPro	IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR001849. Pleckstrin_homology. IPR001936. RasGAP. IPR023152. RasGAP_CS. IPR008936. Rho_GTPase_activation_prot. [Graphical view]
Pfam	PF00616. RasGAP. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00323. RasGAP. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50004. C2. False negative. PS50003. PH_DOMAIN. False negative. PS00509. RAS_GTPASE_ACTIV_1. 1 hit. PS50018. RAS_GTPASE_ACTIV_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	396821.
SOURCE	Search...

Entry information

Entry name

RASL3_MOUSE

Accession

Primary (citable) accession number: Q8C2K5
Secondary accession number(s): A3KMM0 , Q8C2A5, Q8C9R4, Q8CDB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program