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Protein

WASH complex subunit strumpellin

Gene

Kiaa0196

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting. May be involved in axonal outgrowth. Involved in cellular localization of ADRB2. Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (By similarity). Involved in cytokinesis and following polar body extrusion during oocyte meiotic maturation (PubMed:24998208).By similarity1 Publication

GO - Biological processi

  • endosomal transport Source: MGI
  • oocyte maturation Source: UniProtKB
  • polar body extrusion after meiotic divisions Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • spindle assembly involved in meiosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
WASH complex subunit strumpellin
Gene namesi
Name:Kiaa0196
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2146110. E430025E21Rik.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Endoplasmic reticulum By similarity
  • Early endosome By similarity

  • Note: Colocalizes with SYP/synaptophysin in the external molecular layer of the dentate gyrus and in motoneurons of the ventral horn of spinal cord.By similarity

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • early endosome Source: MGI
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • endosome Source: MGI
  • WASH complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000507341 – 1159WASH complex subunit strumpellinAdd BLAST1159

Proteomic databases

EPDiQ8C2E7.
PaxDbiQ8C2E7.
PeptideAtlasiQ8C2E7.
PRIDEiQ8C2E7.

PTM databases

iPTMnetiQ8C2E7.
PhosphoSitePlusiQ8C2E7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022350.
CleanExiMM_E430025E21RIK.
GenevisibleiQ8C2E7. MM.

Interactioni

Subunit structurei

Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASH1, FAM21, KIAA1033/SWIP, KIAA0196/strumpellin and CCDC53. The WASH core complex associates via FAM21 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex. Interacts with VCP, PI4K2A (By similarity).By similarity

Protein-protein interaction databases

BioGridi230156. 1 interactor.
STRINGi10090.ENSMUSP00000022976.

Structurei

3D structure databases

ProteinModelPortaliQ8C2E7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the strumpellin family.Curated

Phylogenomic databases

eggNOGiKOG3666. Eukaryota.
ENOG410XNSS. LUCA.
GeneTreeiENSGT00390000011137.
HOGENOMiHOG000258245.
HOVERGENiHBG102793.
InParanoidiQ8C2E7.
KOiK18464.
OMAiMLYVCLY.
OrthoDBiEOG091G02GF.
PhylomeDBiQ8C2E7.
TreeFamiTF314480.

Family and domain databases

InterProiIPR019393. WASH_strumpellin.
[Graphical view]
PANTHERiPTHR15691. PTHR15691. 1 hit.
PfamiPF10266. Strumpellin. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C2E7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLDFLAENNL CGQAILRIVS CGNAIIAEVL RLSEFIPAVF LLKDRADQQR
60 70 80 90 100
YGDIIFDFSY FKGPEFWESK LEAKPELQDL DEEFRENNIE IVTRFYLAFQ
110 120 130 140 150
SVHKYIVDLN RYLDDLNEGV YIQQTLETVL LSEDGKQLLC EALYLYGVML
160 170 180 190 200
LVIDQKIEGE VRERMLVSYY RYSAARSSAD SNMDDICKLL RSTGYSSQPG
210 220 230 240 250
AKRPPNYPES YFQRVPINET FISMVIGRLR SDDIYNQVSA YPLPEHRSTA
260 270 280 290 300
LANQAAMLYV ILYFEPSILH THQAKMREIV DKYFPDNWVI SIYMGITVNL
310 320 330 340 350
ADAWEPYKAA KTALNNTLDL ANVKEQASRY ASVSDRVRAQ VQQFLKEGYL
360 370 380 390 400
REEVLLDNIP RLLNCLRDCN VAIRWLMLHT ADSACDPNNK RLRQIKDQIL
410 420 430 440 450
ADSRYNPKIL FQLLLDTAQF EFILKEMFKQ MLSEKQSKWE HYKKEGSERM
460 470 480 490 500
TELADVFSGV KPLTRVEKNE NLQAWFREIS KQILSLNYDD STAAGRKTVQ
510 520 530 540 550
LIQALEEVQE FHQLESNLQV CQFLADTRKF LHQMIRTINI KEEVLITVQI
560 570 580 590 600
IGDLSFAWQL IDSFTSIMQE SIRVNPSMVT KLRATFLKLA SALDLPLLRI
610 620 630 640 650
NQANSPDLLS VSQYYSGELV SYVRKVLQII PESMFTSLLK IIKLQTHDIM
660 670 680 690 700
EVPTRLDKDK LRDYAQLGPR YEVAKLTHAI SIFTEGILMM KTTLVGIIKV
710 720 730 740 750
DPKQLLEDGI RKELVKRVAF ALHRGLIFNP RAKPSELMPK LKELGATMDG
760 770 780 790 800
FHRSFEYIQD YVSIYGLKIW QEEVSRIINY NVEQECNNFL RTKIQDWQSM
810 820 830 840 850
YQSTHIPIPK FAPVDESITF IGRLCREILR ITDPKMTCYI DQLNTWYDVK
860 870 880 890 900
THQEVTSSRL FSEIQTTLGT FGLNGLDRLL CFMIVKELQN FLSMFQKIIL
910 920 930 940 950
KERTVQETLK MLMSAVNPLK SIVANSSKVY LSAITKTQKI WSAYLEAIMK
960 970 980 990 1000
VGQMQILRQQ IANELNSSCR FDSRHLAAAL DNLNKALLAD IEAHYRDPSL
1010 1020 1030 1040 1050
PYPKEDNTLL YEITAYLEAA GIHNPLNKIY ITTKRLPYFP IVNFLFLIAQ
1060 1070 1080 1090 1100
LPKLQYNKNL GMVCRKPADP VDWPPLVLGL LTLLKQFHSR YTEQFLALIG
1110 1120 1130 1140 1150
QFIRSTMEQC TSQKMPEMPA DAVGALLFLE DYVRYTKLPR RVAEAHVPNF

IFDEFRTVL
Length:1,159
Mass (Da):134,110
Last modified:May 10, 2004 - v2
Checksum:iF7E7B5A60340E1BF
GO
Isoform 2 (identifier: Q8C2E7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-450: Missing.
     451-469: TELADVFSGVKPLTRVEKN → MAEPVWRCGCKASGQEEEL

Note: No experimental confirmation available.
Show »
Length:709
Mass (Da):81,751
Checksum:i63C2AD6AD17BC944
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti682I → T in BAC40548 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0103231 – 450Missing in isoform 2. 1 PublicationAdd BLAST450
Alternative sequenceiVSP_010324451 – 469TELAD…RVEKN → MAEPVWRCGCKASGQEEEL in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031364 mRNA. Translation: AAH31364.1.
BC034070 mRNA. Translation: AAH34070.1.
BC040815 mRNA. Translation: AAH40815.1.
BC067035 mRNA. Translation: AAH67035.1.
AK088754 mRNA. Translation: BAC40548.1.
AK129086 Transcribed RNA. Translation: BAC97896.2.
CCDSiCCDS37083.1. [Q8C2E7-1]
RefSeqiNP_705776.2. NM_153548.2. [Q8C2E7-1]
UniGeneiMm.218665.

Genome annotation databases

EnsembliENSMUST00000022976; ENSMUSP00000022976; ENSMUSG00000022350. [Q8C2E7-1]
GeneIDi223593.
KEGGimmu:223593.
UCSCiuc007vxs.1. mouse. [Q8C2E7-2]
uc007vxt.1. mouse. [Q8C2E7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC031364 mRNA. Translation: AAH31364.1.
BC034070 mRNA. Translation: AAH34070.1.
BC040815 mRNA. Translation: AAH40815.1.
BC067035 mRNA. Translation: AAH67035.1.
AK088754 mRNA. Translation: BAC40548.1.
AK129086 Transcribed RNA. Translation: BAC97896.2.
CCDSiCCDS37083.1. [Q8C2E7-1]
RefSeqiNP_705776.2. NM_153548.2. [Q8C2E7-1]
UniGeneiMm.218665.

3D structure databases

ProteinModelPortaliQ8C2E7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230156. 1 interactor.
STRINGi10090.ENSMUSP00000022976.

PTM databases

iPTMnetiQ8C2E7.
PhosphoSitePlusiQ8C2E7.

Proteomic databases

EPDiQ8C2E7.
PaxDbiQ8C2E7.
PeptideAtlasiQ8C2E7.
PRIDEiQ8C2E7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022976; ENSMUSP00000022976; ENSMUSG00000022350. [Q8C2E7-1]
GeneIDi223593.
KEGGimmu:223593.
UCSCiuc007vxs.1. mouse. [Q8C2E7-2]
uc007vxt.1. mouse. [Q8C2E7-1]

Organism-specific databases

MGIiMGI:2146110. E430025E21Rik.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3666. Eukaryota.
ENOG410XNSS. LUCA.
GeneTreeiENSGT00390000011137.
HOGENOMiHOG000258245.
HOVERGENiHBG102793.
InParanoidiQ8C2E7.
KOiK18464.
OMAiMLYVCLY.
OrthoDBiEOG091G02GF.
PhylomeDBiQ8C2E7.
TreeFamiTF314480.

Miscellaneous databases

PROiQ8C2E7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022350.
CleanExiMM_E430025E21RIK.
GenevisibleiQ8C2E7. MM.

Family and domain databases

InterProiIPR019393. WASH_strumpellin.
[Graphical view]
PANTHERiPTHR15691. PTHR15691. 1 hit.
PfamiPF10266. Strumpellin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTRUM_MOUSE
AccessioniPrimary (citable) accession number: Q8C2E7
Secondary accession number(s): Q8BGY1, Q8K2J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 2, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.