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Q8C267 (SETB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase SETDB2

EC=2.1.1.43
Alternative name(s):
SET domain bifurcated 2
Gene names
Name:Setdb2
Synonyms:Gm293
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length713 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis By similarity.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subcellular location

Nucleus By similarity. Chromosome By similarity.

Domain

In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Contains 1 MBD (methyl-CpG-binding) domain.

Contains 1 pre-SET domain.

Contains 1 SET domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8C267-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8C267-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-86: GMFITYSNPEVNTHRSN → D
     119-156: SPGKKVFLPV...HRHICSRTCL → YVYVIRVSAP...NSRCLCLLVN
     157-713: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 713713Histone-lysine N-methyltransferase SETDB2
PRO_0000281824

Regions

Domain161 – 23373MBD
Domain294 – 36774Pre-SET
Domain370 – 688319SET
Region380 – 3823S-adenosyl-L-methionine binding By similarity
Region645 – 6462S-adenosyl-L-methionine binding By similarity

Sites

Metal binding2961Zinc 1 By similarity
Metal binding2961Zinc 2 By similarity
Metal binding2981Zinc 1 By similarity
Metal binding3021Zinc 1 By similarity
Metal binding3021Zinc 3 By similarity
Metal binding3081Zinc 1 By similarity
Metal binding3101Zinc 2 By similarity
Metal binding3481Zinc 2 By similarity
Metal binding3481Zinc 3 By similarity
Metal binding3521Zinc 2 By similarity
Metal binding3541Zinc 3 By similarity
Metal binding3591Zinc 3 By similarity
Metal binding6481Zinc 4 By similarity
Metal binding7011Zinc 4 By similarity
Metal binding7031Zinc 4 By similarity
Metal binding7081Zinc 4 By similarity
Binding site6421S-adenosyl-L-methionine By similarity

Natural variations

Alternative sequence70 – 8617GMFIT…THRSN → D in isoform 2.
VSP_024063
Alternative sequence119 – 15638SPGKK…SRTCL → YVYVIRVSAPSVCCLLNIPK SLTPFIKFNSRCLCLLVN in isoform 2.
VSP_024064
Alternative sequence157 – 713557Missing in isoform 2.
VSP_024065

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 3, 2007. Version 2.
Checksum: C4E750F01ED4231A

FASTA71380,636
        10         20         30         40         50         60 
MEEKNGDAKT FWMELQDDGK VDLMFEKTQN VLHSLKQKIK DGSATNGDYV QAMNLVNEAT 

        70         80         90        100        110        120 
LSNTQTLEKG MFITYSNPEV NTHRSNHTPV TQSEQENKSS AVPSASCDNS CPKGCTIPSP 

       130        140        150        160        170        180 
GKKVFLPVKN KADNLVKKEA PLHISFHRHI CSRTCLMETP LSLKGENPLQ LPIRCHFQRR 

       190        200        210        220        230        240 
HAKTNSHSSA LHVNYKTPCG RNLRNMEEVF HYLLETECNF LFTDNFSFNT YVQLTRNHPK 

       250        260        270        280        290        300 
QNEVVSDVDI SNGVESVSIP FCNEIDNSKL PRFKYRNTVW PRIYHLNFSN MFSDSCDCSE 

       310        320        330        340        350        360 
GCIDIKKCAC LQLTAKNAKA CPLSSDGECA GYKYKRLQRL IPTGIYECNL LCKCNKQMCQ 

       370        380        390        400        410        420 
NRVIQHGVRV RLQVFKSEKK GWGVRCLDDI DKGTFVCIYS GRLLRRATPE KTNIGENGRE 

       430        440        450        460        470        480 
QQHIVKNSFS KKRKLEVVCS DCDAHCDSPK AEDCPPKLSG DLKEPAVEMN HRNISRTQHH 

       490        500        510        520        530        540 
SVIRRTKSKT TVFHYSEKNM GFVCSDSAAP EDKNGFKPAQ EHVNSEARRA HEDLSSNPAG 

       550        560        570        580        590        600 
DSEDTQLTES DVIDITASRE DSAPAYRCKH ATIVDRKDTK QVLEVPGKKS QEEEPAASQS 

       610        620        630        640        650        660 
QQALCDEELP SERTKIPSAS LMQLSKESLF LLDASKEGNV GRFLNHSCCP NLWVQNVFVE 

       670        680        690        700        710 
THDRNFPLVA FFTNRYVKAR TELTWDYGYE AGATPAKEIL CQCGFNKCRK KLI 

« Hide

Isoform 2 [UniParc].

Checksum: 37E19B746B1A9205
Show »

FASTA14015,493

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: NOD.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK089197 mRNA. Translation: BAC40789.1.
AC114007 Genomic DNA. No translation available.
CCDSCCDS36939.1. [Q8C267-1]
RefSeqNP_001074493.1. NM_001081024.1. [Q8C267-1]
UniGeneMm.205022.

3D structure databases

ProteinModelPortalQ8C267.
SMRQ8C267. Positions 247-414, 629-709.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000093450.

PTM databases

PhosphoSiteQ8C267.

Proteomic databases

PaxDbQ8C267.
PRIDEQ8C267.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000095775; ENSMUSP00000093450; ENSMUSG00000071350. [Q8C267-1]
ENSMUST00000111253; ENSMUSP00000106884; ENSMUSG00000071350. [Q8C267-2]
GeneID239122.
KEGGmmu:239122.
UCSCuc007uei.1. mouse. [Q8C267-1]
uc007uej.1. mouse. [Q8C267-2]

Organism-specific databases

CTD83852.
MGIMGI:2685139. Setdb2.

Phylogenomic databases

eggNOGCOG2940.
GeneTreeENSGT00750000117355.
HOGENOMHOG000060314.
HOVERGENHBG106688.
InParanoidQ8C267.
KOK11421.
OMAKCHFQRR.
PhylomeDBQ8C267.
TreeFamTF106411.

Gene expression databases

ArrayExpressQ8C267.
BgeeQ8C267.
CleanExMM_SETDB2.
GenevestigatorQ8C267.

Family and domain databases

InterProIPR016177. DNA-bd_dom.
IPR001739. Methyl_CpG_DNA-bd.
IPR007728. Pre-SET_dom.
IPR001214. SET_dom.
[Graphical view]
PfamPF01429. MBD. 1 hit.
PF05033. Pre-SET. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTSM00317. SET. 1 hit.
[Graphical view]
SUPFAMSSF54171. SSF54171. 1 hit.
PROSITEPS50982. MBD. 1 hit.
PS50867. PRE_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio383999.
PROQ8C267.
SOURCESearch...

Entry information

Entry nameSETB2_MOUSE
AccessionPrimary (citable) accession number: Q8C267
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: July 9, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot