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Protein

Sodium/potassium/calcium exchanger 5

Gene

Slc24a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cation exchanger involved in pigmentation, possibly by participating in ion transport in melanosomes. Predominant sodium-Calcium exchanger in melanocytes. Probably transports 1 Ca2+ and 1 K+ to the melanosome in exchange for 4 cytoplasmic Na+ (By similarity).By similarity

GO - Molecular functioni

  1. calcium, potassium:sodium antiporter activity Source: MGI
  2. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. ion transmembrane transport Source: MGI
  2. negative regulation of melanin biosynthetic process Source: MGI
  3. response to stimulus Source: UniProtKB-KW
  4. sodium ion transmembrane transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Antiport, Calcium transport, Ion transport, Potassium transport, Sensory transduction, Sodium transport, Symport, Transport

Keywords - Ligandi

Calcium, Potassium, Sodium

Enzyme and pathway databases

ReactomeiREACT_236234. Sodium/Calcium exchangers.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium/calcium exchanger 5
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 5
Solute carrier family 24 member 5
Gene namesi
Name:Slc24a5
Synonyms:Nckx5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2677271. Slc24a5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 6637ExtracellularSequence AnalysisAdd
BLAST
Transmembranei67 – 8721Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini88 – 11124CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei112 – 13221Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini133 – 1364ExtracellularSequence Analysis
Transmembranei137 – 15721Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini158 – 17013CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei171 – 19121Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini192 – 1943ExtracellularSequence Analysis
Transmembranei195 – 21521Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini216 – 30590CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei306 – 32621Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini327 – 3348ExtracellularSequence Analysis
Transmembranei335 – 35521Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini356 – 3627CytoplasmicSequence Analysis
Transmembranei363 – 38321Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini384 – 40017ExtracellularSequence AnalysisAdd
BLAST
Transmembranei401 – 42121Helical; Name=9Sequence AnalysisAdd
BLAST
Topological domaini422 – 43817CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei439 – 45921Helical; Name=10Sequence AnalysisAdd
BLAST
Topological domaini460 – 47011ExtracellularSequence AnalysisAdd
BLAST
Transmembranei471 – 49121Helical; Name=11Sequence AnalysisAdd
BLAST
Topological domaini492 – 50110CytoplasmicSequence Analysis

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. melanosome Source: UniProtKB-SubCell
  3. trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 501472Sodium/potassium/calcium exchanger 5PRO_0000045754Add
BLAST

Proteomic databases

MaxQBiQ8C261.
PRIDEiQ8C261.

Expressioni

Tissue specificityi

Highly expressed in melanin-producing cells such as skin and eye compared to other tissues. Strongly overexpressed in melanoma cell lines. Expressed in dental tissues.2 Publications

Gene expression databases

BgeeiQ8C261.
CleanExiMM_SLC24A5.
ExpressionAtlasiQ8C261. baseline.
GenevestigatoriQ8C261.

Structurei

3D structure databases

ProteinModelPortaliQ8C261.
SMRiQ8C261. Positions 84-215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0530.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000231933.
HOVERGENiHBG054881.
InParanoidiQ8C261.
KOiK13753.
OMAiKGLTYIT.
OrthoDBiEOG776SP8.
PhylomeDBiQ8C261.
TreeFamiTF318759.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
IPR030245. SLC24A5.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 1 hit.
PTHR10846:SF27. PTHR10846:SF27. 1 hit.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00367. TIGR00367. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8C261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTKWGPTWT RRVLLLGIFW VSAYLPVRGV SLPPRLPRAT GNSTQCAVSP
60 70 80 90 100
ASEFPEGFFT KQESTDGGIV IYFLIILYMC MAISIVCDKY FLPSLEIISD
110 120 130 140 150
SLGLSQDVAG ATFMAAGSSA PELVTAFLGV FITKGDIGIS TILGSAIYNL
160 170 180 190 200
LGICAACGLL SNMVSTLSCW PLFRDCAVYA VSVGAVFGII FDNRIYWYEG
210 220 230 240 250
AGLLLIYGLY VLLLCFDTTI SRHVMKTCSP CCPCLARAME ERIEQQTLLG
260 270 280 290 300
WEDESQLFIR RQSRTDSGIF QEDSGYSQLS LSLHGLSQVS EDPPSVFSMP
310 320 330 340 350
EADLRRIFWV LSLPIITLLA LTTPDCRRKF WKNYFVITFF MSALWISAFT
360 370 380 390 400
YILVWMVTVT GETLGIPDTV MGLTLLAAGT SIPDTVTSVL VARKGKGDMA
410 420 430 440 450
ISNIVGSNVF DMLCLGLPWF IKTAFTNASA PIEVNSKGLT YITISLNISI
460 470 480 490 500
LFLFLAVHFN GWKLDRKLGV VCLVLYLGLA TLSVLYEIGI IGNNRIRGCG

V
Length:501
Mass (Da):54,984
Last modified:March 1, 2003 - v1
Checksum:iB0AFD2BE7FC7AB34
GO

Sequence cautioni

The sequence BAC24122.1 differs from that shown. Reason: Frameshift at positions 5 and 14. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401T → K in BAC24122 (Ref. 2) Curated
Sequence conflicti97 – 971I → V in AAP57526 (Ref. 1) Curated
Sequence conflicti120 – 1201A → G in BAC24122 (Ref. 2) Curated
Sequence conflicti342 – 3421S → C in BAC24122 (Ref. 2) Curated
Sequence conflicti474 – 4741V → L in BAC24122 (Ref. 2) Curated
Sequence conflicti488 – 4881I → V in BAC24122 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY236527 mRNA. Translation: AAP57526.1.
AB085629 mRNA. Translation: BAC24122.1. Frameshift.
AK089225 mRNA. Translation: BAC40800.1.
BC094232 mRNA. Translation: AAH94232.1.
CCDSiCCDS38226.1.
RefSeqiNP_778199.2. NM_175034.3.
UniGeneiMm.330538.

Genome annotation databases

EnsembliENSMUST00000070353; ENSMUSP00000063887; ENSMUSG00000035183.
GeneIDi317750.
KEGGimmu:317750.
UCSCiuc008mbv.1. mouse.

Cross-referencesi

Web resourcesi

Protein Spotlight

Skin-deep - Issue 74 of September 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY236527 mRNA. Translation: AAP57526.1.
AB085629 mRNA. Translation: BAC24122.1. Frameshift.
AK089225 mRNA. Translation: BAC40800.1.
BC094232 mRNA. Translation: AAH94232.1.
CCDSiCCDS38226.1.
RefSeqiNP_778199.2. NM_175034.3.
UniGeneiMm.330538.

3D structure databases

ProteinModelPortaliQ8C261.
SMRiQ8C261. Positions 84-215.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiQ8C261.
PRIDEiQ8C261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070353; ENSMUSP00000063887; ENSMUSG00000035183.
GeneIDi317750.
KEGGimmu:317750.
UCSCiuc008mbv.1. mouse.

Organism-specific databases

CTDi283652.
MGIiMGI:2677271. Slc24a5.

Phylogenomic databases

eggNOGiCOG0530.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000231933.
HOVERGENiHBG054881.
InParanoidiQ8C261.
KOiK13753.
OMAiKGLTYIT.
OrthoDBiEOG776SP8.
PhylomeDBiQ8C261.
TreeFamiTF318759.

Enzyme and pathway databases

ReactomeiREACT_236234. Sodium/Calcium exchangers.

Miscellaneous databases

NextBioi394160.
PROiQ8C261.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C261.
CleanExiMM_SLC24A5.
ExpressionAtlasiQ8C261. baseline.
GenevestigatoriQ8C261.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
IPR030245. SLC24A5.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 1 hit.
PTHR10846:SF27. PTHR10846:SF27. 1 hit.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00367. TIGR00367. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a novel, mouse renal divalent cation transporter (JSX)."
    Satoh J., Smith C., Romero M.F.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "Cloning of a new member of Na-Ca exchanger."
    Ishibashi K.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.
  5. Cited for: TISSUE SPECIFICITY.
  6. "Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts."
    Hu P., Lacruz R.S., Smith C.E., Smith S.M., Kurtz I., Paine M.L.
    Cells Tissues Organs 196:501-509(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiNCKX5_MOUSE
AccessioniPrimary (citable) accession number: Q8C261
Secondary accession number(s): Q6XE39, Q8CJF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 1, 2003
Last modified: March 4, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.