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Q8C261 (NCKX5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/potassium/calcium exchanger 5
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 5
Solute carrier family 24 member 5
Gene names
Name:Slc24a5
Synonyms:Nckx5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cation exchanger involved in pigmentation, possibly by participating in ion transport in melanosomes. Predominant sodium-Calcium exchanger in melanocytes. Probably transports 1 Ca2+ and 1 K+ to the melanosome in exchange for 4 cytoplasmic Na+ By similarity.

Subcellular location

Golgi apparatustrans-Golgi network membrane; Multi-pass membrane protein By similarity. Melanosome By similarity.

Tissue specificity

Highly expressed in melanin-producing cells such as skin and eye compared to other tissues. Strongly overexpressed in melanoma cell lines. Expressed in dental tissues. Ref.5 Ref.6

Sequence similarities

Belongs to the sodium/potassium/calcium exchanger family. SLC24A subfamily.

Sequence caution

The sequence BAC24122.1 differs from that shown. Reason: Frameshift at positions 5 and 14.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 501472Sodium/potassium/calcium exchanger 5
PRO_0000045754

Regions

Topological domain30 – 6637Extracellular Potential
Transmembrane67 – 8721Helical; Name=1; Potential
Topological domain88 – 11124Cytoplasmic Potential
Transmembrane112 – 13221Helical; Name=2; Potential
Topological domain133 – 1364Extracellular Potential
Transmembrane137 – 15721Helical; Name=3; Potential
Topological domain158 – 17013Cytoplasmic Potential
Transmembrane171 – 19121Helical; Name=4; Potential
Topological domain192 – 1943Extracellular Potential
Transmembrane195 – 21521Helical; Name=5; Potential
Topological domain216 – 30590Cytoplasmic Potential
Transmembrane306 – 32621Helical; Name=6; Potential
Topological domain327 – 3348Extracellular Potential
Transmembrane335 – 35521Helical; Name=7; Potential
Topological domain356 – 3627Cytoplasmic Potential
Transmembrane363 – 38321Helical; Name=8; Potential
Topological domain384 – 40017Extracellular Potential
Transmembrane401 – 42121Helical; Name=9; Potential
Topological domain422 – 43817Cytoplasmic Potential
Transmembrane439 – 45921Helical; Name=10; Potential
Topological domain460 – 47011Extracellular Potential
Transmembrane471 – 49121Helical; Name=11; Potential
Topological domain492 – 50110Cytoplasmic Potential

Experimental info

Sequence conflict401T → K in BAC24122. Ref.2
Sequence conflict971I → V in AAP57526. Ref.1
Sequence conflict1201A → G in BAC24122. Ref.2
Sequence conflict3421S → C in BAC24122. Ref.2
Sequence conflict4741V → L in BAC24122. Ref.2
Sequence conflict4881I → V in BAC24122. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8C261 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: B0AFD2BE7FC7AB34

FASTA50154,984
        10         20         30         40         50         60 
MRTKWGPTWT RRVLLLGIFW VSAYLPVRGV SLPPRLPRAT GNSTQCAVSP ASEFPEGFFT 

        70         80         90        100        110        120 
KQESTDGGIV IYFLIILYMC MAISIVCDKY FLPSLEIISD SLGLSQDVAG ATFMAAGSSA 

       130        140        150        160        170        180 
PELVTAFLGV FITKGDIGIS TILGSAIYNL LGICAACGLL SNMVSTLSCW PLFRDCAVYA 

       190        200        210        220        230        240 
VSVGAVFGII FDNRIYWYEG AGLLLIYGLY VLLLCFDTTI SRHVMKTCSP CCPCLARAME 

       250        260        270        280        290        300 
ERIEQQTLLG WEDESQLFIR RQSRTDSGIF QEDSGYSQLS LSLHGLSQVS EDPPSVFSMP 

       310        320        330        340        350        360 
EADLRRIFWV LSLPIITLLA LTTPDCRRKF WKNYFVITFF MSALWISAFT YILVWMVTVT 

       370        380        390        400        410        420 
GETLGIPDTV MGLTLLAAGT SIPDTVTSVL VARKGKGDMA ISNIVGSNVF DMLCLGLPWF 

       430        440        450        460        470        480 
IKTAFTNASA PIEVNSKGLT YITISLNISI LFLFLAVHFN GWKLDRKLGV VCLVLYLGLA 

       490        500 
TLSVLYEIGI IGNNRIRGCG V 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel, mouse renal divalent cation transporter (JSX)."
Satoh J., Smith C., Romero M.F.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Cloning of a new member of Na-Ca exchanger."
Ishibashi K.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NOD.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Eye.
[5]"SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans."
Lamason R.L., Mohideen M.-A.P.K., Mest J.R., Wong A.C., Norton H.L., Aros M.C., Jurynec M.J., Mao X., Humphreville V.R., Humbert J.E., Sinha S., Moore J.L., Jagadeeswaran P., Zhao W., Ning G., Makalowska I., McKeigue P.M., O'Donnell D. expand/collapse author list , Kittles R., Parra E.J., Mangini N.J., Grunwald D.J., Shriver M.D., Canfield V.A., Cheng K.C.
Science 310:1782-1786(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts."
Hu P., Lacruz R.S., Smith C.E., Smith S.M., Kurtz I., Paine M.L.
Cells Tissues Organs 196:501-509(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Skin-deep - Issue 74 of September 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY236527 mRNA. Translation: AAP57526.1.
AB085629 mRNA. Translation: BAC24122.1. Frameshift.
AK089225 mRNA. Translation: BAC40800.1.
BC094232 mRNA. Translation: AAH94232.1.
CCDSCCDS38226.1.
RefSeqNP_778199.2. NM_175034.3.
UniGeneMm.330538.

3D structure databases

ProteinModelPortalQ8C261.
SMRQ8C261. Positions 84-215.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8C261.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070353; ENSMUSP00000063887; ENSMUSG00000035183.
GeneID317750.
KEGGmmu:317750.
UCSCuc008mbv.1. mouse.

Organism-specific databases

CTD283652.
MGIMGI:2677271. Slc24a5.

Phylogenomic databases

eggNOGCOG0530.
GeneTreeENSGT00730000110432.
HOGENOMHOG000231933.
HOVERGENHBG054881.
InParanoidQ8C261.
KOK13753.
OMAYITIFLN.
OrthoDBEOG776SP8.
PhylomeDBQ8C261.
TreeFamTF318759.

Gene expression databases

BgeeQ8C261.
CleanExMM_SLC24A5.
GenevestigatorQ8C261.

Family and domain databases

InterProIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
[Graphical view]
PANTHERPTHR10846. PTHR10846. 1 hit.
PfamPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsTIGR00367. TIGR00367. 1 hit.
ProtoNetSearch...

Other

NextBio394160.
PROQ8C261.
SOURCESearch...

Entry information

Entry nameNCKX5_MOUSE
AccessionPrimary (citable) accession number: Q8C261
Secondary accession number(s): Q6XE39, Q8CJF1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot