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Protein

Dipeptidase 2

Gene

Dpep2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable metalloprotease which hydrolyzes leukotriene D4 (LTD4) into leukotriene E4 (LTE4).

Catalytic activityi

Hydrolysis of dipeptides.PROSITE-ProRule annotation

Cofactori

Zn2+PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by L-penicillamine.1 Publication

Kineticsi

  1. KM=5 µM for LTD41 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi91 – 911Zinc 1; catalyticPROSITE-ProRule annotation
Metal bindingi93 – 931Zinc 1; catalyticPROSITE-ProRule annotation
Metal bindingi190 – 1901Zinc 1; catalyticPROSITE-ProRule annotation
Metal bindingi190 – 1901Zinc 2; catalyticPROSITE-ProRule annotation
Binding sitei217 – 2171SubstratePROSITE-ProRule annotation
Metal bindingi263 – 2631Zinc 2; catalyticPROSITE-ProRule annotation
Metal bindingi284 – 2841Zinc 2; catalyticPROSITE-ProRule annotation
Binding sitei295 – 2951SubstratePROSITE-ProRule annotation
Binding sitei353 – 3531SubstratePROSITE-ProRule annotation

GO - Molecular functioni

  1. dipeptidase activity Source: UniProtKB-KW
  2. dipeptidyl-peptidase activity Source: InterPro
  3. exopeptidase activity Source: MGI
  4. metal ion binding Source: UniProtKB-KW
  5. metalloexopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM19.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidase 2 (EC:3.4.13.19)
Alternative name(s):
Membrane-bound dipeptidase 2
Short name:
MBD-2
Gene namesi
Name:Dpep2
Synonyms:Mbd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:2442042. Dpep2.

Subcellular locationi

Membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 459428Dipeptidase 2PRO_0000231605Add
BLAST
Propeptidei460 – 47819Removed in mature formSequence AnalysisPRO_0000231606Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi113 – 1131N-linked (GlcNAc...)By similarity
Disulfide bondi140 ↔ 219PROSITE-ProRule annotation
Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi291 ↔ 323PROSITE-ProRule annotation
Disulfide bondi428 – 428InterchainPROSITE-ProRule annotation
Lipidationi459 – 4591GPI-anchor amidated serineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ8C255.
PaxDbiQ8C255.
PRIDEiQ8C255.

Expressioni

Tissue specificityi

Expressed in heart, lung, testis, spleen and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ8C255.
CleanExiMM_DPEP2.
MM_MBD2.
GenevestigatoriQ8C255.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.PROSITE-ProRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8C255.
SMRiQ8C255. Positions 74-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M19 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2355.
GeneTreeiENSGT00390000017920.
HOGENOMiHOG000072016.
HOVERGENiHBG002339.
InParanoidiQ8C255.
OMAiVDILETC.
OrthoDBiEOG7SJD4N.
PhylomeDBiQ8C255.
TreeFamiTF324523.

Family and domain databases

InterProiIPR000180. Dipep_AS.
IPR028531. Dpep2.
IPR008257. Renal_dipep_fam.
[Graphical view]
PANTHERiPTHR10443. PTHR10443. 1 hit.
PTHR10443:SF9. PTHR10443:SF9. 1 hit.
PfamiPF01244. Peptidase_M19. 1 hit.
[Graphical view]
PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C255-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLTGLKGHW VLGHGLSVFL LVLLLLGPSQ PLIWTQTKPG FSGASTTSSI
60 70 80 90 100
PRALTKPDIS SIPTTPGNPN FPDLRDRARA LMQEFPLIDG HNDMPLVLRQ
110 120 130 140 150
FYQNGLQDAN LRNFTHGQTS LDRLKDGLVG AQFWSAYVPC QTQDRDALRL
160 170 180 190 200
TLEQIDLIRR ICASYSELEL VTSVKALNST QKLACLIGVE GGHSLDNSLA
210 220 230 240 250
VLRSFYLLGV RYLTLTHTCN TPWAETSSKG VHAFYSSVTG LTSFGEKVVA
260 270 280 290 300
EMNRLGMMVD LSHVSDAAAR RALEVSQAPV IFSHSAARAV CPNARNLPDD
310 320 330 340 350
LLQLLKKNGG IVMVTFSVGV LPCNPLANVS TVADHFDHIR SVIGSEFIGI
360 370 380 390 400
GGDYDGTKQF PQGLEDVSTY PVLIEELLRR GWNEQELQGI LRGNLLRVFR
410 420 430 440 450
QVEQVRDKSK WQSPLEDMIP EEQLDSACHS ALRPQKQHPE KNQPETPEYH
460 470
ILKFSHSKSS PHIVPSLAIV ATLLGLIV
Length:478
Mass (Da):52,664
Last modified:March 1, 2003 - v1
Checksum:i0790B97009F5D41F
GO
Isoform 2 (identifier: Q8C255-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAWHGETYCL...VPACSALSKM
     176-179: ALNS → VKWIYSG
     334-340: DHFDHIR → EKGQQKHQFTPPRDTHFV

Show »
Length:578
Mass (Da):63,675
Checksum:i253DC762000875A4
GO
Isoform 3 (identifier: Q8C255-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: Missing.

Show »
Length:385
Mass (Da):42,667
Checksum:iD28E45A1A5169543
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781A → T in BAE42354 (PubMed:16141072).Curated
Sequence conflicti89 – 891D → G in BAE42559 (PubMed:16141072).Curated
Sequence conflicti99 – 991R → G in BAE42559 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9393Missing in isoform 3. 1 PublicationVSP_017852Add
BLAST
Alternative sequencei1 – 11M → MAWHGETYCLIGGYRVYGDA PLPTPAKAEQEEKPVPRRAP KRQRVQEESDQDLGCPGAKV PRLKLKHGGKGLSRPSSVPA CSALSKM in isoform 2. 1 PublicationVSP_017853
Alternative sequencei176 – 1794ALNS → VKWIYSG in isoform 2. 1 PublicationVSP_017854
Alternative sequencei334 – 3407DHFDHIR → EKGQQKHQFTPPRDTHFV in isoform 2. 1 PublicationVSP_017855

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF488552 mRNA. Translation: AAP84986.1.
AK089244 mRNA. Translation: BAC40810.1.
AK155417 mRNA. Translation: BAE33255.1.
AK170094 mRNA. Translation: BAE41559.1.
AK170970 mRNA. Translation: BAE42149.1.
AK171044 mRNA. Translation: BAE42209.1.
AK171122 mRNA. Translation: BAE42264.1.
AK171155 mRNA. Translation: BAE42280.1.
AK171196 mRNA. Translation: BAE42306.1.
AK171268 mRNA. Translation: BAE42354.1.
AK171503 mRNA. Translation: BAE42494.1.
AK171606 mRNA. Translation: BAE42559.1.
CCDSiCCDS22625.1. [Q8C255-2]
RefSeqiNP_001288133.1. NM_001301204.1. [Q8C255-1]
NP_001288134.1. NM_001301205.1. [Q8C255-3]
NP_795887.2. NM_176913.4. [Q8C255-2]
UniGeneiMm.248357.
Mm.470084.

Genome annotation databases

EnsembliENSMUST00000034373; ENSMUSP00000034373; ENSMUSG00000053687. [Q8C255-1]
ENSMUST00000081998; ENSMUSP00000080659; ENSMUSG00000053687. [Q8C255-2]
ENSMUST00000117555; ENSMUSP00000113877; ENSMUSG00000053687. [Q8C255-3]
GeneIDi319446.
KEGGimmu:319446.
UCSCiuc009nev.1. mouse. [Q8C255-3]
uc009ney.1. mouse. [Q8C255-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF488552 mRNA. Translation: AAP84986.1.
AK089244 mRNA. Translation: BAC40810.1.
AK155417 mRNA. Translation: BAE33255.1.
AK170094 mRNA. Translation: BAE41559.1.
AK170970 mRNA. Translation: BAE42149.1.
AK171044 mRNA. Translation: BAE42209.1.
AK171122 mRNA. Translation: BAE42264.1.
AK171155 mRNA. Translation: BAE42280.1.
AK171196 mRNA. Translation: BAE42306.1.
AK171268 mRNA. Translation: BAE42354.1.
AK171503 mRNA. Translation: BAE42494.1.
AK171606 mRNA. Translation: BAE42559.1.
CCDSiCCDS22625.1. [Q8C255-2]
RefSeqiNP_001288133.1. NM_001301204.1. [Q8C255-1]
NP_001288134.1. NM_001301205.1. [Q8C255-3]
NP_795887.2. NM_176913.4. [Q8C255-2]
UniGeneiMm.248357.
Mm.470084.

3D structure databases

ProteinModelPortaliQ8C255.
SMRiQ8C255. Positions 74-430.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM19.002.

Proteomic databases

MaxQBiQ8C255.
PaxDbiQ8C255.
PRIDEiQ8C255.

Protocols and materials databases

DNASUi319446.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034373; ENSMUSP00000034373; ENSMUSG00000053687. [Q8C255-1]
ENSMUST00000081998; ENSMUSP00000080659; ENSMUSG00000053687. [Q8C255-2]
ENSMUST00000117555; ENSMUSP00000113877; ENSMUSG00000053687. [Q8C255-3]
GeneIDi319446.
KEGGimmu:319446.
UCSCiuc009nev.1. mouse. [Q8C255-3]
uc009ney.1. mouse. [Q8C255-2]

Organism-specific databases

CTDi64174.
MGIiMGI:2442042. Dpep2.

Phylogenomic databases

eggNOGiCOG2355.
GeneTreeiENSGT00390000017920.
HOGENOMiHOG000072016.
HOVERGENiHBG002339.
InParanoidiQ8C255.
OMAiVDILETC.
OrthoDBiEOG7SJD4N.
PhylomeDBiQ8C255.
TreeFamiTF324523.

Miscellaneous databases

NextBioi394746.
PROiQ8C255.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C255.
CleanExiMM_DPEP2.
MM_MBD2.
GenevestigatoriQ8C255.

Family and domain databases

InterProiIPR000180. Dipep_AS.
IPR028531. Dpep2.
IPR008257. Renal_dipep_fam.
[Graphical view]
PANTHERiPTHR10443. PTHR10443. 1 hit.
PTHR10443:SF9. PTHR10443:SF9. 1 hit.
PfamiPF01244. Peptidase_M19. 1 hit.
[Graphical view]
PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of two additional members of the membrane-bound dipeptidase family."
    Habib G.M., Shi Z.-Z., Cuevas A.A., Lieberman M.W.
    FASEB J. 17:1313-1315(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION.
    Strain: C57BL/6.
    Tissue: Heart, Lung and Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Dendritic cell.

Entry informationi

Entry nameiDPEP2_MOUSE
AccessioniPrimary (citable) accession number: Q8C255
Secondary accession number(s): Q3TAV9
, Q3TBF7, Q3TBK3, Q3TBM9, Q7TQ53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: March 1, 2003
Last modified: March 4, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.