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UniProtKB - Q8C255 (DPEP2_MOUSE)

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Protein

Dipeptidase 2

Gene

Dpep2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable metalloprotease which hydrolyzes leukotriene D4 (LTD4) into leukotriene E4 (LTE4).

Catalytic activityi

Hydrolysis of dipeptides.PROSITE-ProRule annotation

Cofactori

Zn2+PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by L-penicillamine.1 Publication

Kineticsi

  1. KM=5 µM for LTD41 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi91Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi93Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi190Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi190Zinc 2; catalyticPROSITE-ProRule annotation1
    Binding sitei217SubstratePROSITE-ProRule annotation1
    Metal bindingi263Zinc 2; catalyticPROSITE-ProRule annotation1
    Metal bindingi284Zinc 2; catalyticPROSITE-ProRule annotation1
    Binding sitei295SubstratePROSITE-ProRule annotation1
    Binding sitei353SubstratePROSITE-ProRule annotation1

    GO - Molecular functioni

    Complete GO annotation...

    Keywordsi

    Molecular functionDipeptidase, Hydrolase, Metalloprotease, Protease
    LigandMetal-binding, Zinc

    Protein family/group databases

    MEROPSiM19.002.

    Chemistry databases

    SwissLipidsiSLP:000001462.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dipeptidase 2 (EC:3.4.13.19)
    Alternative name(s):
    Membrane-bound dipeptidase 2
    Short name:
    MBD-2
    Gene namesi
    Name:Dpep2
    Synonyms:Mbd2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:2442042. Dpep2.

    Subcellular locationi

    • Membrane 1 Publication; Lipid-anchor › GPI-anchor 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 31Sequence analysisAdd BLAST31
    ChainiPRO_000023160532 – 459Dipeptidase 2Add BLAST428
    PropeptideiPRO_0000231606460 – 478Removed in mature formSequence analysisAdd BLAST19

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi113N-linked (GlcNAc...) asparagineBy similarity1
    Disulfide bondi140 ↔ 219PROSITE-ProRule annotation
    Glycosylationi178N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi291 ↔ 323PROSITE-ProRule annotation
    Disulfide bondi428InterchainPROSITE-ProRule annotation
    Lipidationi459GPI-anchor amidated serineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiQ8C255.
    PeptideAtlasiQ8C255.
    PRIDEiQ8C255.

    PTM databases

    PhosphoSitePlusiQ8C255.

    Expressioni

    Tissue specificityi

    Expressed in heart, lung, testis, spleen and skeletal muscle.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000053687.
    CleanExiMM_DPEP2.
    MM_MBD2.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.PROSITE-ProRule annotation

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000080659.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8C255.
    SMRiQ8C255.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG4127. Eukaryota.
    COG2355. LUCA.
    GeneTreeiENSGT00390000017920.
    HOGENOMiHOG000072016.
    HOVERGENiHBG002339.
    InParanoidiQ8C255.
    KOiK01273.
    OrthoDBiEOG091G09CU.
    PhylomeDBiQ8C255.
    TreeFamiTF324523.

    Family and domain databases

    CDDicd01301. rDP_like. 1 hit.
    InterProiView protein in InterPro
    IPR000180. Dipep_AS.
    IPR032466. Metal_Hydrolase.
    IPR008257. Renal_dipep_fam.
    PANTHERiPTHR10443. PTHR10443. 1 hit.
    PfamiView protein in Pfam
    PF01244. Peptidase_M19. 1 hit.
    SUPFAMiSSF51556. SSF51556. 1 hit.
    PROSITEiView protein in PROSITE
    PS00869. RENAL_DIPEPTIDASE_1. 1 hit.
    PS51365. RENAL_DIPEPTIDASE_2. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q8C255-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MSLTGLKGHW VLGHGLSVFL LVLLLLGPSQ PLIWTQTKPG FSGASTTSSI 
            60         70         80         90        100
    PRALTKPDIS SIPTTPGNPN FPDLRDRARA LMQEFPLIDG HNDMPLVLRQ 
           110        120        130        140        150
    FYQNGLQDAN LRNFTHGQTS LDRLKDGLVG AQFWSAYVPC QTQDRDALRL 
           160        170        180        190        200
    TLEQIDLIRR ICASYSELEL VTSVKALNST QKLACLIGVE GGHSLDNSLA 
           210        220        230        240        250
    VLRSFYLLGV RYLTLTHTCN TPWAETSSKG VHAFYSSVTG LTSFGEKVVA 
           260        270        280        290        300
    EMNRLGMMVD LSHVSDAAAR RALEVSQAPV IFSHSAARAV CPNARNLPDD 
           310        320        330        340        350
    LLQLLKKNGG IVMVTFSVGV LPCNPLANVS TVADHFDHIR SVIGSEFIGI 
           360        370        380        390        400
    GGDYDGTKQF PQGLEDVSTY PVLIEELLRR GWNEQELQGI LRGNLLRVFR 
           410        420        430        440        450
    QVEQVRDKSK WQSPLEDMIP EEQLDSACHS ALRPQKQHPE KNQPETPEYH 
           460        470 
    ILKFSHSKSS PHIVPSLAIV ATLLGLIV
    Length:478
    Mass (Da):52,664
    Last modified:March 1, 2003 - v1
    Checksum:i0790B97009F5D41F
    GO
    Isoform 2 (identifier: Q8C255-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MAWHGETYCL...VPACSALSKM
         176-179: ALNS → VKWIYSG
         334-340: DHFDHIR → EKGQQKHQFTPPRDTHFV

    Show »
    Length:578
    Mass (Da):63,675
    Checksum:i253DC762000875A4
    GO
    Isoform 3 (identifier: Q8C255-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-93: Missing.

    Show »
    Length:385
    Mass (Da):42,667
    Checksum:iD28E45A1A5169543
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti78A → T in BAE42354 (PubMed:16141072).Curated1
    Sequence conflicti89D → G in BAE42559 (PubMed:16141072).Curated1
    Sequence conflicti99R → G in BAE42559 (PubMed:16141072).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0178521 – 93Missing in isoform 3. 1 PublicationAdd BLAST93
    Alternative sequenceiVSP_0178531M → MAWHGETYCLIGGYRVYGDA PLPTPAKAEQEEKPVPRRAP KRQRVQEESDQDLGCPGAKV PRLKLKHGGKGLSRPSSVPA CSALSKM in isoform 2. 1 Publication1
    Alternative sequenceiVSP_017854176 – 179ALNS → VKWIYSG in isoform 2. 1 Publication4
    Alternative sequenceiVSP_017855334 – 340DHFDHIR → EKGQQKHQFTPPRDTHFV in isoform 2. 1 Publication7

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		AF488552 mRNA. Translation: AAP84986.1.
    AK089244 mRNA. Translation: BAC40810.1.
    AK155417 mRNA. Translation: BAE33255.1.
    AK170094 mRNA. Translation: BAE41559.1.
    AK170970 mRNA. Translation: BAE42149.1.
    AK171044 mRNA. Translation: BAE42209.1.
    AK171122 mRNA. Translation: BAE42264.1.
    AK171155 mRNA. Translation: BAE42280.1.
    AK171196 mRNA. Translation: BAE42306.1.
    AK171268 mRNA. Translation: BAE42354.1.
    AK171503 mRNA. Translation: BAE42494.1.
    AK171606 mRNA. Translation: BAE42559.1.
    CCDSiCCDS22625.1. [Q8C255-2]
    CCDS80925.1. [Q8C255-3]
    CCDS80926.1. [Q8C255-1]
    RefSeqiNP_001288133.1. NM_001301204.1. [Q8C255-1]
    NP_001288134.1. NM_001301205.1. [Q8C255-3]
    NP_795887.2. NM_176913.4. [Q8C255-2]
    UniGeneiMm.248357.
    Mm.470084.

    Genome annotation databases

    EnsembliENSMUST00000034373; ENSMUSP00000034373; ENSMUSG00000053687. [Q8C255-1]
    ENSMUST00000081998; ENSMUSP00000080659; ENSMUSG00000053687. [Q8C255-2]
    ENSMUST00000117555; ENSMUSP00000113877; ENSMUSG00000053687. [Q8C255-3]
    GeneIDi319446.
    KEGGimmu:319446.
    UCSCiuc009nex.2. mouse. [Q8C255-3]
    uc009ney.2. mouse. [Q8C255-2]
    uc057amr.1. mouse. [Q8C255-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiDPEP2_MOUSE
    AccessioniPrimary (citable) accession number: Q8C255
    Secondary accession number(s): Q3TAV9
    , Q3TBF7, Q3TBK3, Q3TBM9, Q7TQ53
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: March 1, 2003
    Last modified: July 5, 2017
    This is version 107 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families