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Protein

Zinc finger protein Eos

Gene

Ikzf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. Essential for the inhibitory function of regulatory T-cells (Treg). Mediates FOXP3-mediated gene silencing in regulatory T-cells (Treg) via recruitment of corepressor CTBP1 (PubMed:19696312).3 Publications

Miscellaneous

Eos is a Greek word for rising sun.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 181C2H2-type 1Add BLAST23
Zinc fingeri187 – 209C2H2-type 2Add BLAST23
Zinc fingeri215 – 237C2H2-type 3Add BLAST23
Zinc fingeri248 – 271C2H2-type 4Add BLAST24
Zinc fingeri531 – 553C2H2-type 5Add BLAST23
Zinc fingeri559 – 583C2H2-type 6Add BLAST25

GO - Molecular functioni

  • bHLH transcription factor binding Source: MGI
  • protein domain specific binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI
  • sequence-specific DNA binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: GO_Central
  • transcription regulatory region DNA binding Source: GO_Central
  • zinc ion binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein Eos
Alternative name(s):
Ikaros family zinc finger protein 4
Gene namesi
Name:Ikzf4
Synonyms:Zfpn1a4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1343139. Ikzf4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002994691 – 586Zinc finger protein EosAdd BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki100Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei105PhosphoserineBy similarity1
Modified residuei335N6-acetyllysineBy similarity1
Cross-linki501Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8C208.
PRIDEiQ8C208.

PTM databases

iPTMnetiQ8C208.
PhosphoSitePlusiQ8C208.

Expressioni

Tissue specificityi

Expressed mainly in the brain. Up-regulated in long term cultured astrocytes. Down-regulated during osteoclast differentiation.2 Publications

Developmental stagei

Highly expressed in the brain at E15 and E18. Expression gradually decreased as postnatal development proceeded. No expression detected in the liver, kidney, lung, thymus or spleen during all developmental stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000002578.
CleanExiMM_IKZF4.
ExpressionAtlasiQ8C208. baseline and differential.
GenevisibleiQ8C208. MM.

Interactioni

Subunit structurei

Self-associates. Interacts with other family members; IKZF1, IKZF2, IKZF3 AND IKZF5 (By similarity). Interacts with CTBP2, SPI1 and MITF. Interacts with FOXP3 and CTBP1.By similarity4 Publications

GO - Molecular functioni

  • bHLH transcription factor binding Source: MGI
  • protein domain specific binding Source: MGI
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi204703. 1 interactor.
STRINGi10090.ENSMUSP00000114404.

Structurei

3D structure databases

ProteinModelPortaliQ8C208.
SMRiQ8C208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni281 – 586Interaction with FOXP31 PublicationAdd BLAST306

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi423 – 433CTBP-binding motif PEDLGAdd BLAST11

Domaini

The N-terminal zinc fingers are involved in sequence-specific DNA binding.
C-terminal zinc fingers mediate homodimerization.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri159 – 181C2H2-type 1Add BLAST23
Zinc fingeri187 – 209C2H2-type 2Add BLAST23
Zinc fingeri215 – 237C2H2-type 3Add BLAST23
Zinc fingeri248 – 271C2H2-type 4Add BLAST24
Zinc fingeri531 – 553C2H2-type 5Add BLAST23
Zinc fingeri559 – 583C2H2-type 6Add BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00550000074392.
HOGENOMiHOG000049114.
HOVERGENiHBG004752.
InParanoidiQ8C208.
KOiK09220.
OMAiYHARAPL.
OrthoDBiEOG091G05E2.
PhylomeDBiQ8C208.
TreeFamiTF331189.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR036236. Znf_C2H2_sf.
IPR013087. Znf_C2H2_type.
IPR013083. Znf_RING/FYVE/PHD.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 6 hits.
SUPFAMiSSF57667. SSF57667. 3 hits.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 5 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C208-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHTPPALPRR FQGGGRVRTP GSHRQGKDNL ERELSGGCAP DFLPQAQDSN
60 70 80 90 100
HFIMESLFCE SSGDSSLEKE FLGAPVGPSV STPNSQHSSP SRSLSANSIK
110 120 130 140 150
VEMYSDEESS RLLGPDERLL DKDDSVIVED SLSEPLGYCD GSGPEPHSPG
160 170 180 190 200
GIRLPNGKLK CDVCGMVCIG PNVLMVHKRS HTGERPFHCN QCGASFTQKG
210 220 230 240 250
NLLRHIKLHS GEKPFKCPFC NYACRRRDAL TGHLRTHSVS SPTVGKPYKC
260 270 280 290 300
NYCGRSYKQQ STLEEHKERC HNYLQSLSTD AQALTGQPGD EIRDLEMVPD
310 320 330 340 350
SMLHPSTERP TFIDRLANSL TKRKRSTPQK FVGEKQMRFS LSDLPYDVNA
360 370 380 390 400
SGGYEKDVEL VAHHGLEPGF GGSLAFVGTE HLRPLRLPPT NCISELTPVI
410 420 430 440 450
SSVYTQMQPI PSRLELPGSR EAGEGPEDLG DGGPLLYRAR GSLTDPGASP
460 470 480 490 500
SNGCQDSTDT ESNHEDRIGG VVSLPQGPPP QPPPTIVVGR HSPAYAKEDP
510 520 530 540 550
KPQEGLLRGT PGPSKEVLRV VGESGEPVKA FKCEHCRILF LDHVMFTIHM
560 570 580
GCHGFRDPFE CNICGYHSQD RYEFSSHIVR GEHKVG
Length:586
Mass (Da):64,155
Last modified:September 11, 2007 - v2
Checksum:iBC39FF5BBE7F914C
GO
Isoform 2 (identifier: Q8C208-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:533
Mass (Da):58,335
Checksum:iBDFCCC00C23C5746
GO
Isoform 3 (identifier: Q8C208-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: MHTPPALPRRFQGGGRVRTPGSHRQGKDNLERELSGGCAPDFLPQAQDSNHFIMESLFCES → MFIPVG

Note: No experimental confirmation available.
Show »
Length:531
Mass (Da):58,052
Checksum:i7DA51FC4203BC451
GO
Isoform 4 (identifier: Q8C208-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     572-586: YEFSSHIVRGEHKVG → TRRLVPRLLG...SSIAGGSLSL

Note: No experimental confirmation available.
Show »
Length:686
Mass (Da):75,075
Checksum:iAAF4318A96EA1307
GO

Sequence cautioni

The sequence BAA36213 differs from that shown. Reason: Frameshift at positions 472 and 479.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70 – 71EF → DS in BAA36213 (PubMed:10218586).Curated2
Sequence conflicti291E → V in BAE41808 (PubMed:16141072).Curated1
Sequence conflicti352G → S in BAE41808 (PubMed:16141072).Curated1
Sequence conflicti478P → T in BAC40912 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0276891 – 61MHTPP…LFCES → MFIPVG in isoform 3. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_0276901 – 53Missing in isoform 2. 2 PublicationsAdd BLAST53
Alternative sequenceiVSP_027691572 – 586YEFSS…EHKVG → TRRLVPRLLGPVMINGREKG DVSFLSANFQYNQKNCPRMN YTYVPVNHSTLVPARMGRTQ LGVTSTALSILSSRHRAGEA VFSGGCRHSGYSDNRGFVRP CRRRHSSIAGGSLSL in isoform 4. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017615 mRNA. Translation: BAA36213.1. Frameshift.
AK089522 mRNA. Translation: BAC40912.1.
AK170453 mRNA. Translation: BAE41808.1.
AC117232 Genomic DNA. No translation available.
BC141286 mRNA. Translation: AAI41287.1.
CCDSiCCDS24284.2. [Q8C208-1]
RefSeqiNP_035902.2. NM_011772.2. [Q8C208-1]
XP_006513677.1. XM_006513614.2. [Q8C208-3]
XP_006513678.1. XM_006513615.2. [Q8C208-3]
XP_011241744.1. XM_011243442.2. [Q8C208-2]
UniGeneiMm.358648.

Genome annotation databases

EnsembliENSMUST00000133342; ENSMUSP00000114404; ENSMUSG00000002578. [Q8C208-1]
ENSMUST00000221150; ENSMUSP00000152617; ENSMUSG00000002578. [Q8C208-4]
ENSMUST00000222067; ENSMUSP00000152234; ENSMUSG00000002578. [Q8C208-2]
GeneIDi22781.
KEGGimmu:22781.
UCSCiuc007hnp.1. mouse. [Q8C208-1]
uc011xqg.1. mouse. [Q8C208-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIKZF4_MOUSE
AccessioniPrimary (citable) accession number: Q8C208
Secondary accession number(s): B9EJ21, Q3TCZ7, Q9Z2Z2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: October 25, 2017
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families