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Protein

Mediator of RNA polymerase II transcription subunit 19

Gene

Med19

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 19
Alternative name(s):
Mediator complex subunit 19
Gene namesi
Name:Med19
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914234. Med19.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244Mediator of RNA polymerase II transcription subunit 19PRO_0000304767Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei194 – 1941PhosphoserineBy similarity
Modified residuei226 – 2261PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8C1S0.
MaxQBiQ8C1S0.
PaxDbiQ8C1S0.
PeptideAtlasiQ8C1S0.
PRIDEiQ8C1S0.

PTM databases

iPTMnetiQ8C1S0.
PhosphoSiteiQ8C1S0.

Expressioni

Gene expression databases

BgeeiQ8C1S0.
GenevisibleiQ8C1S0. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MED26O954022EBI-398761,EBI-394392From a different organism.
Med31Q9CXU12EBI-398761,EBI-309355

GO - Molecular functioni

Protein-protein interaction databases

BioGridi237911. 2 interactions.
IntActiQ8C1S0. 11 interactions.
MINTiMINT-4114078.
STRINGi10090.ENSMUSP00000099705.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 4532Pro-richAdd
BLAST
Compositional biasi172 – 22251Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 19 family.Curated

Phylogenomic databases

eggNOGiKOG4043. Eukaryota.
ENOG4111KKA. LUCA.
GeneTreeiENSGT00390000001774.
HOGENOMiHOG000047323.
HOVERGENiHBG055317.
InParanoidiQ8C1S0.
KOiK15137.
OMAiNRHSPEH.
OrthoDBiEOG7XSTG9.
PhylomeDBiQ8C1S0.
TreeFamiTF317417.

Family and domain databases

InterProiIPR019403. Mediator_Med19_met.
[Graphical view]
PfamiPF10278. Med19. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C1S0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENFTALFGA QTDPPPPPSA LGFGPGKPPP PPPPPPGGGP GAAPPSTATS
60 70 80 90 100
APAGADKSTA GSGPFYLMRE LPGSTELTGS TNLITHYNLE QAYNKFCGKK
110 120 130 140 150
VKEKLSNFLP DLPGMIDLPG SHDNSSLRSL IEKPPILGGS FNPITGTMLS
160 170 180 190 200
GFRLHTGPLP EQCRLMHIQP PKKKNKHKHK QSRTQDPVPP ETPSDSDHKK
210 220 230 240
KKKKKEEDPE RKRKKKEKKK KKNRHSPDHP GMGSSQASSS SSLR
Length:244
Mass (Da):26,263
Last modified:March 1, 2003 - v1
Checksum:i6F38036D801490AE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 14012SLIEK…ILGGS → HASAHASAHASA in AAH25475 (PubMed:15489334).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005399 mRNA. Translation: BAC25114.1.
AK160579 mRNA. Translation: BAE35886.1.
AK168787 mRNA. Translation: BAE40621.1.
BC025475 mRNA. Translation: AAH25475.1.
BC044207 mRNA. Translation: AAH44207.1.
BC082559 mRNA. Translation: AAH82559.1.
CCDSiCCDS16189.1.
RefSeqiNP_080161.1. NM_025885.3.
UniGeneiMm.219643.

Genome annotation databases

EnsembliENSMUST00000102645; ENSMUSP00000099705; ENSMUSG00000027080.
GeneIDi381379.
KEGGimmu:381379.
UCSCiuc008kiy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005399 mRNA. Translation: BAC25114.1.
AK160579 mRNA. Translation: BAE35886.1.
AK168787 mRNA. Translation: BAE40621.1.
BC025475 mRNA. Translation: AAH25475.1.
BC044207 mRNA. Translation: AAH44207.1.
BC082559 mRNA. Translation: AAH82559.1.
CCDSiCCDS16189.1.
RefSeqiNP_080161.1. NM_025885.3.
UniGeneiMm.219643.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237911. 2 interactions.
IntActiQ8C1S0. 11 interactions.
MINTiMINT-4114078.
STRINGi10090.ENSMUSP00000099705.

PTM databases

iPTMnetiQ8C1S0.
PhosphoSiteiQ8C1S0.

Proteomic databases

EPDiQ8C1S0.
MaxQBiQ8C1S0.
PaxDbiQ8C1S0.
PeptideAtlasiQ8C1S0.
PRIDEiQ8C1S0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102645; ENSMUSP00000099705; ENSMUSG00000027080.
GeneIDi381379.
KEGGimmu:381379.
UCSCiuc008kiy.2. mouse.

Organism-specific databases

CTDi219541.
MGIiMGI:1914234. Med19.

Phylogenomic databases

eggNOGiKOG4043. Eukaryota.
ENOG4111KKA. LUCA.
GeneTreeiENSGT00390000001774.
HOGENOMiHOG000047323.
HOVERGENiHBG055317.
InParanoidiQ8C1S0.
KOiK15137.
OMAiNRHSPEH.
OrthoDBiEOG7XSTG9.
PhylomeDBiQ8C1S0.
TreeFamiTF317417.

Enzyme and pathway databases

ReactomeiR-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiMed19. mouse.
PROiQ8C1S0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C1S0.
GenevisibleiQ8C1S0. MM.

Family and domain databases

InterProiIPR019403. Mediator_Med19_met.
[Graphical view]
PfamiPF10278. Med19. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell, Heart and Placenta.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Eye, Liver and Mammary tumor.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney and Spleen.

Entry informationi

Entry nameiMED19_MOUSE
AccessioniPrimary (citable) accession number: Q8C1S0
Secondary accession number(s): Q80XM2, Q8R3G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.