Reviewed, UniProtKB/Swiss-Prot Q8C1B2 (PARPT_MOUSE)

Names and origin

Protein names

Recommended name:
    TCDD-inducible poly [ADP-ribose] polymerase
    EC=2.4.2.30

Gene names

Name:

Tiparp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Poly [ADP-ribose] polymerase using NAD+ as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals. Ref.1
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Tissue specificity	Ubiquitously expressed. Ref.1
Induction	By 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which is a by-product of industrial processes and an important environmental contaminant. This induction is concentration and time-dependent and is mediated through an aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocation (Arnt) signal transduction. Superinduced by cycloheximide and by inhibitors of the 26S proteasome. Ref.1 Ref.4
Sequence similarities	Contains 1 C3H1-type zinc finger. Contains 1 PARP catalytic domain. Contains 1 WWE domain.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	Metal-binding NAD Zinc
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	face morphogenesis Inferred from mutant phenotype. Source: MGI hemopoiesis Inferred from mutant phenotype. Source: MGI kidney development Inferred from mutant phenotype. Source: MGI multicellular organismal metabolic process Inferred from mutant phenotype. Source: MGI nitrogen compound metabolic process Inferred from mutant phenotype. Source: MGI palate development Inferred from mutant phenotype. Source: MGI platelet-derived growth factor receptor signaling pathway Inferred from mutant phenotype. Source: MGI post-embryonic development Inferred from mutant phenotype. Source: MGI protein amino acid ADP-ribosylation Ref.1 Inferred from direct assay. Source: MGI skeletal system morphogenesis Inferred from mutant phenotype. Source: MGI smooth muscle tissue development Inferred from mutant phenotype. Source: MGI vasculogenesis Inferred from mutant phenotype. Source: MGI
Molecular function	NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	TCDD-inducible poly [ADP-ribose] polymerase		PRO_0000247836
Regions
	Domain	333 – 411	79	WWE
	Domain	449 – 657	209	PARP catalytic
	Zinc finger	238 – 265	28	C3H1-type
Experimental info
	Sequence conflict	521	1	R → H in BAC27876. Ref.2
	Sequence conflict	620	1	D → N in BAC27876. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8C1B2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 332CB9721C7EBC33	FASTA	657	75,901
	10 20 30 40 50 60 MEVETTEPEP DCVVQPPSPS DDFSCQMRIS EKISPLKTCF KKKQEQKRLG TGTLRSLRPI 70 80 90 100 110 120 LNTLLESGSL DGVFRARDQN RDESSLHEHI VKKPLEINPS CPPAENSMPV LIPDGTNVEG 130 140 150 160 170 180 QLPEAHPSTD APEQGVPIQD HSFPPETISG TVADSTTGHF QTDLLHPVSG DVPTSPDCVD 190 200 210 220 230 240 KVMDYVPGAF QDNSFTIQYI LDTSDKLSTE LFQDKSEEAS LELVFELVNQ LQYHTHQENG 250 260 270 280 290 300 IEICMDFLQG TCIYGRDCLK HHTVLPYHWQ IKRTTTQKWQ SVSNDSQEHL ERFYCNPEND 310 320 330 340 350 360 RMRMKYGGQD FWADLNAMTV FETTEFDQLR RLSTPPCSNS NSIYHTFWKF FCRDHFGWRE 370 380 390 400 410 420 YPESVVRLIE EANSRGLKEV RFMMWNNHYI LHNSFFRREI KRRPLFRSCF ILIPYLQTLG 430 440 450 460 470 480 GVPTQASLPL EATSSQIICP DGVTSANFYP ETWVYMHPSQ DFIQVPVSAE DKSYRIIYNL 490 500 510 520 530 540 FHKTVPEFKY RILQILRVQN QFLWEKYKRK KEYMNRKMSG RDRIINERHL FHGTSQDVVD 550 560 570 580 590 600 GICKHNFDPR VCGKHATMFG QGSYFAKKAS YSHNFSKKSS KGVHFMFLAK VLTGRYTMGS 610 620 630 640 650 HGMRRPPPVN PGSVTSDLYD SCVDNFFEPQ IFVIFNDDQS YPYFVIQYEE VSNTVSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TCDD-inducible poly(ADP-ribose) polymerase: a novel response to 2,3,7,8-tetrachlorodibenzo-p-dioxin." Ma Q., Baldwin K.T., Renzelli A.J., McDaniel A., Dong L. Biochem. Biophys. Res. Commun. 289:499-506(2001) [PubMed: 11716501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow, Olfactory bulb and Skin.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Eye.
[4]	"Induction and superinduction of 2,3,7,8-tetrachlorodibenzo-rho-dioxin-inducible poly(ADP-ribose) polymerase: role of the aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator transcription activation domains and a labile transcription repressor." Ma Q. Arch. Biochem. Biophys. 404:309-316(2002) [PubMed: 12147270] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK028529 mRNA. Translation: BAC25994.1. AK032453 mRNA. Translation: BAC27876.1. AK150248 mRNA. Translation: BAE29412.1. AK162094 mRNA. Translation: BAE36720.1. BC068173 mRNA. Translation: AAH68173.1.
IPI	IPI00453574.
RefSeq	NP_849223.2.
UniGene	Mm.246398
3D structure databases
SMR	Q8C1B2. Positions 261-404, 460-652.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8C1B2.
Proteomic databases
PRIDE	Q8C1B2.
Genome annotation databases
Ensembl	ENSMUST00000047906; ENSMUSP00000048051; ENSMUSG00000034640; Mus musculus. [Genome view]
GeneID	99929.
KEGG	mmu:99929.
UCSC	uc008pkr.1. mouse.
Organism-specific databases
CTD	99929.
MGI	MGI:2159210. Tiparp.
Phylogenomic databases
eggNOG	roNOG09894.
HOGENOM	HBG283791.
HOVERGEN	Q8C1B2.
InParanoid	Q8C1B2.
OMA	DSQEHLE.
OrthoDB	EOG9ZKN6F.
PhylomeDB	Q8C1B2.
Enzyme and pathway databases
BRENDA	2.4.2.30. 244.
Gene expression databases
ArrayExpress	Q8C1B2.
Bgee	Q8C1B2.
CleanEx	MM_TIPARP.
Genevestigator	Q8C1B2.
GermOnline	ENSMUSG00000034640. Mus musculus.
Family and domain databases
InterPro	IPR012317. Poly(ADP-ribose)pol_cat_dom. IPR004170. WWE-dom. IPR000571. Znf_CCCH. [Graphical view]
Pfam	PF00644. PARP. 1 hit. [Graphical view]
PROSITE	PS51059. PARP_CATALYTIC. 1 hit. PS50918. WWE. 1 hit. PS50103. ZF_C3H1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	354169.
SOURCE	Search...

Entry information

Entry name

PARPT_MOUSE

Accession

Primary (citable) accession number: Q8C1B2
Secondary accession number(s): Q3UD50, Q8C032

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	March 1, 2003
Last modified:	January 19, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)