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Protein

Calmodulin-regulated spectrin-associated protein 2

Gene

Camsap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:23169647). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (By similarity). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (By similarity). In addition, it also reduces the velocity of microtubule polymerization (By similarity). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (By similarity). Essential for the tethering, but not for nucleation of non-centrosomal microtubules at the Golgi: together with Golgi-associated proteins AKAP9 and PDE4DIP, required to tether non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (By similarity). Also acts as a regulator of neuronal polarity and development: localizes to non-centrosomal microtubule minus-ends in neurons and stabilizes non-centrosomal microtubules, which is required for neuronal polarity, axon specification and dendritic branch formation (By similarity). Through the microtubule cytoskeleton, regulates the autophagosome transport (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-regulated spectrin-associated protein 2Curated
Alternative name(s):
Calmodulin-regulated spectrin-associated protein 1-like protein 1
Gene namesi
Name:Camsap2Imported
Synonyms:Camsap1l1, Kiaa10781 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1922434. Camsap2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003168331 – 1461Calmodulin-regulated spectrin-associated protein 2Add BLAST1461

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei391PhosphoserineCombined sources1
Modified residuei393PhosphoserineCombined sources1
Modified residuei401PhosphothreonineCombined sources1
Modified residuei439PhosphoserineCombined sources1
Modified residuei572PhosphoserineBy similarity1
Modified residuei573PhosphoserineCombined sources1
Modified residuei585PhosphoserineCombined sources1
Modified residuei647PhosphoserineBy similarity1
Modified residuei652PhosphothreonineCombined sources1
Modified residuei654PhosphoserineCombined sources1
Modified residuei836PhosphoserineCombined sources1
Modified residuei905PhosphoserineBy similarity1
Modified residuei910PhosphoserineBy similarity1
Modified residuei970PhosphothreonineBy similarity1
Modified residuei975PhosphothreonineBy similarity1
Modified residuei977PhosphothreonineBy similarity1
Modified residuei981PhosphoserineBy similarity1
Modified residuei992PhosphoserineBy similarity1
Modified residuei1120PhosphoserineCombined sources1
Modified residuei1285PhosphoserineCombined sources1
Modified residuei1291PhosphoserineCombined sources1
Modified residuei1293PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8C1B1.
PaxDbiQ8C1B1.
PeptideAtlasiQ8C1B1.
PRIDEiQ8C1B1.

PTM databases

iPTMnetiQ8C1B1.
PhosphoSitePlusiQ8C1B1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041570.
ExpressionAtlasiQ8C1B1. baseline and differential.
GenevisibleiQ8C1B1. MM.

Interactioni

Subunit structurei

Interacts with CAMSAP3 (PubMed:23169647). Interacts with KATNA1 and KATNB1; leading to regulate the length of CAMSAP2-decorated microtubule stretches (By similarity). Interacts with a complex formed by AKAP9 and PDE4DIP isoform 2/MMG8/SMYLE, which recruits CAMSAP2 to the Golgi (By similarity). Interacts with MAPRE1/EB1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Camsap3Q80VC92EBI-8839434,EBI-2125556

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212510. 2 interactors.
DIPiDIP-60092N.
IntActiQ8C1B1. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ8C1B1.
SMRiQ8C1B1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 324Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST114
Domaini1321 – 1455CKKPROSITE-ProRule annotationAdd BLAST135

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni896 – 1007MBD regionBy similarityAdd BLAST112

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili730 – 767Sequence analysisAdd BLAST38
Coiled coili861 – 900Sequence analysisAdd BLAST40
Coiled coili1138 – 1210Sequence analysisAdd BLAST73

Domaini

The CKK domain binds microtubules and specifically recognizes the minus-end of microtubules.PROSITE-ProRule annotationBy similarity
The MBD (microtubule-binding domain) region can recognize some features of the microtubule lattice, which might contribute to the specific decoration of growing microtubule minus-ends by CAMSAP2.By similarity

Sequence similaritiesi

Belongs to the CAMSAP1 family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IU7J. Eukaryota.
ENOG411033G. LUCA.
GeneTreeiENSGT00390000010026.
HOVERGENiHBG107572.
InParanoidiQ8C1B1.
KOiK17493.
PhylomeDBiQ8C1B1.

Family and domain databases

Gene3Di3.10.20.360. 1 hit.
InterProiView protein in InterPro
IPR032940. CAMSAP.
IPR031372. CAMSAP_CC1.
IPR022613. CAMSAP_CH.
IPR036872. CH_dom_sf.
IPR038209. CKK_dom_sf.
IPR014797. CKK_domain.
IPR011033. PRC_barrel-like_sf.
PANTHERiPTHR21595. PTHR21595. 1 hit.
PfamiView protein in Pfam
PF17095. CAMSAP_CC1. 1 hit.
PF11971. CAMSAP_CH. 1 hit.
PF08683. CAMSAP_CKK. 1 hit.
SMARTiView protein in SMART
SM01051. CAMSAP_CKK. 1 hit.
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF50346. SSF50346. 1 hit.
PROSITEiView protein in PROSITE
PS50021. CH. 1 hit.
PS51508. CKK. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C1B1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDAADPREM RRTFIVPAIK PFDHYDFSRA KIACNLAWLV AKAFGTENVP
60 70 80 90 100
EELGDPFYTD QYDQEHIKPP VVNLLLSAEL YCRAGSLILK SDAAKPLLGH
110 120 130 140 150
DAVIQALAQK GLYVTDQEKL VTERDLHKKP IQMSAHLAMI DTLMMAYTVE
160 170 180 190 200
MISIEKVIAC AQQYSAFFQA TDLPYDIEDA VMYWMNKVNE HLKDIMEQEQ
210 220 230 240 250
KSKEHHPAEA PGGQKARYRK EQTLLKQLPC IPLVENLLKD GTDGCALAAL
260 270 280 290 300
IHFYCPAVVR LEDICLKETM SLADSLYNLQ LIQEFCQEYL NHCCHFSLED
310 320 330 340 350
MLYAASSIKS NYLVFMAELF WWFEVVKPSF VQPRVVRPQG AEPAKDVPSV
360 370 380 390 400
PVLNAAKRNI RDSSSSSDFS SRYTRPQTHS SASGGIRRSS SMSYVDGFIG
410 420 430 440 450
TWPKEKRTSV HGVSFDISFD KEDSAQSSTP NRGIIRSVSN EGLTLNNSRA
460 470 480 490 500
SKHIRKNLSF KPVNGEEEES IEEELHVDPH GDLQSPMPLN TNELNSNEST
510 520 530 540 550
HYKLPNGALQ NRVLLDEFGN QIETPSIEEA LQIIHDTERP PHTPRPDQIA
560 570 580 590 600
NGFFLHGQDL SILNSNIKLN QSSPDNLTDT KGALSPITDT TEVDTGIHVP
610 620 630 640 650
SEDIPETMDE DSSLRDYTVS LDSDMDDASK LLQDYDLRAS NPREALSPCP
660 670 680 690 700
STISTKSQPG SSASSSSGVK MTSFAEQKFR KLNHTDGKSS GSSSQKTTPE
710 720 730 740 750
GSELNIPHVV SWAQIPEEAG VAPGRDTTQL LASEMVHLRM RLEEKRRAIE
760 770 780 790 800
AQKKKMEAAF TKQRQKMGRT AFLTVVKKKG EGISPLREEA AGAEDEKVYT
810 820 830 840 850
DRAKERESQK MDGQRSKSLA DIKESMETPP GRWLKSPTTP VDPERQWNLT
860 870 880 890 900
SPSEETLNEG EILEYTKSIE KLNSSLHFLQ QEMQRLSLQQ EMLMQMREQQ
910 920 930 940 950
AWVISPPQPS PQKQIRDFKP RQAGLSSAAA PFSSDSPRPT HPSPQSSTRK
960 970 980 990 1000
SASFSVKNQR TPRPNELKIT PLNRTLTPPR SVDSLPRLRR FSPSQVPIQT
1010 1020 1030 1040 1050
RSFVCFGDDG EPQKEPKQKE EIKKEPSECK GTLGPCDHNP GEKEIKPVES
1060 1070 1080 1090 1100
TVSEVLSQPI TETVCVTPNE DQLSQPTEPP PKPVFPPTAP KNVNLIEVSL
1110 1120 1130 1140 1150
SDLKPPEKAD VSVEKLDGES DKEQFDDDQK VCCGFFFKDD QKAENDMAMK
1160 1170 1180 1190 1200
RAALLEKRLR REKETQLRKQ QLEAEMEHKK EETRRKTEEE RQKKEDERAR
1210 1220 1230 1240 1250
REFIRQEYMR RKQLKLMEDM DTVIKPRPQA AKQKKQRPKS IHRDHIESPK
1260 1270 1280 1290 1300
TPIKGPPVSS LSLASLNTGD SESVHSGKRT PRSESVEGFL SPSRCGSRNG
1310 1320 1330 1340 1350
EKDWENASTT SSVASGTEYT GPKLYKEPSA KSNKHIIQNA LAHCCLAGKV
1360 1370 1380 1390 1400
NEGQKKKILE EMEKSDANNF LILFRDSGCQ FRSLYTYCPE TEEINKLTGI
1410 1420 1430 1440 1450
GPKSITKKMI EGLYKYNSDR KQFSHIPAKT LSASVDAITI HSHLWQTKRP
1460
VTPKKLLPTK A
Length:1,461
Mass (Da):164,333
Last modified:February 5, 2008 - v3
Checksum:i24308DADC498788A
GO
Isoform 2 (identifier: Q8C1B1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1257-1257: P → PGSRISRVFS

Note: No experimental confirmation available.
Show »
Length:1,470
Mass (Da):165,323
Checksum:iF056D5FAB0D69AFD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30A → T in AAI45259 (PubMed:15489334).Curated1
Sequence conflicti802R → Q in AAI45259 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0308071257P → PGSRISRVFS in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC145258 mRNA. Translation: AAI45259.1.
AK028542 mRNA. Translation: BAC25999.2.
AK081975 mRNA. Translation: BAC38384.1.
AK122436 mRNA. Translation: BAC65718.1.
CCDSiCCDS83597.1. [Q8C1B1-1]
RefSeqiNP_001334039.1. NM_001347110.1. [Q8C1B1-1]
XP_006529897.1. XM_006529834.2. [Q8C1B1-2]
UniGeneiMm.144143.

Genome annotation databases

EnsembliENSMUST00000192001; ENSMUSP00000142166; ENSMUSG00000041570. [Q8C1B1-1]
GeneIDi67886.
KEGGimmu:67886.
UCSCiuc007cur.2. mouse. [Q8C1B1-1]
uc011wsz.1. mouse. [Q8C1B1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCAMP2_MOUSE
AccessioniPrimary (citable) accession number: Q8C1B1
Secondary accession number(s): B7ZNI4, Q80TK8, Q8C4J5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: March 28, 2018
This is version 109 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome