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Q8C1A5

- THOP1_MOUSE

UniProt

Q8C1A5 - THOP1_MOUSE

Protein

Thimet oligopeptidase

Gene

Thop1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein and generate amyloidogenic fragments By similarity.By similarity

    Catalytic activityi

    Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi473 – 4731Zinc; catalyticPROSITE-ProRule annotation
    Active sitei474 – 4741PROSITE-ProRule annotation
    Metal bindingi477 – 4771Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi480 – 4801Zinc; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: MGI
    3. peptide binding Source: Ensembl

    GO - Biological processi

    1. intracellular signal transduction Source: MGI
    2. peptide metabolic process Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM03.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thimet oligopeptidase (EC:3.4.24.15)
    Gene namesi
    Name:Thop1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1354165. Thop1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 687687Thimet oligopeptidasePRO_0000319044Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei257 – 2571N6-acetyllysineBy similarity
    Modified residuei278 – 2781Phosphotyrosine1 Publication
    Modified residuei538 – 5381N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8C1A5.
    PaxDbiQ8C1A5.
    PRIDEiQ8C1A5.

    2D gel databases

    REPRODUCTION-2DPAGEQ8C1A5.

    PTM databases

    PhosphoSiteiQ8C1A5.

    Expressioni

    Gene expression databases

    BgeeiQ8C1A5.
    CleanExiMM_THOP1.
    GenevestigatoriQ8C1A5.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi206040. 2 interactions.
    IntActiQ8C1A5. 1 interaction.
    MINTiMINT-4128941.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8C1A5.
    SMRiQ8C1A5. Positions 24-677.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0339.
    GeneTreeiENSGT00550000074738.
    HOGENOMiHOG000245985.
    HOVERGENiHBG000238.
    InParanoidiQ8C1A5.
    KOiK01392.
    OrthoDBiEOG7SR4KW.
    PhylomeDBiQ8C1A5.
    TreeFamiTF300459.

    Family and domain databases

    Gene3Di1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProiIPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view]
    PfamiPF01432. Peptidase_M3. 1 hit.
    [Graphical view]
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8C1A5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPPAACAGD VVDAASPAST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR    50
    VGAQNFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP CKDIRAASTE 100
    ADKKLSEFDV EMSMRQDVYQ RVVWLQEKTP KNSLKPEAAR YLERLIKLGR 150
    RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK NLNEDTTFLP FTREELGGLP 200
    EDFLSSLEKA EDGKLKVTLK YPHYFPLLKK CHVPETRRLL EEAFNCRCKE 250
    ENCAILKELV SLRAQKSSLL GFHTHADYVL EMNMAKTSQT VATFLDELAQ 300
    KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR 350
    VDQNLLKEYF PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR 400
    DAASGEEIGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQLAVAAMVA 450
    NFTKPTPDAP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF 500
    VEAPSQMLEN WVWEKEPLMR MSQHYRTGSE APQDLLEKLI KSRQANAGLF 550
    NLRQIVLAKV DQVLHTQTDA DPAEEYARLC QEILGVPATP GTNMPATFGH 600
    LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG 650
    SEDASAMLKQ FLGRDPKQDA FLLSKGLQVE GSEAPAC 687
    Length:687
    Mass (Da):78,026
    Last modified:March 1, 2003 - v1
    Checksum:i362D899CE5101662
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti132 – 1321N → D in AAG35061. (PubMed:10969067)Curated
    Sequence conflicti132 – 1321N → D in AAH31722. (PubMed:15489334)Curated
    Sequence conflicti193 – 1942RE → KK in AAG35061. (PubMed:10969067)Curated
    Sequence conflicti205 – 2051S → I in AAG35061. (PubMed:10969067)Curated
    Sequence conflicti529 – 5291S → G in AAG35061. (PubMed:10969067)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF314187 mRNA. Translation: AAG35061.1.
    AK028609 mRNA. Translation: BAC26031.1.
    BC031175 mRNA. Translation: AAH31175.1.
    BC031722 mRNA. Translation: AAH31722.1.
    CCDSiCCDS24042.1.
    RefSeqiNP_073144.3. NM_022653.4.
    UniGeneiMm.26995.

    Genome annotation databases

    EnsembliENSMUST00000005057; ENSMUSP00000005057; ENSMUSG00000004929.
    GeneIDi50492.
    KEGGimmu:50492.
    UCSCiuc007gfv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF314187 mRNA. Translation: AAG35061.1 .
    AK028609 mRNA. Translation: BAC26031.1 .
    BC031175 mRNA. Translation: AAH31175.1 .
    BC031722 mRNA. Translation: AAH31722.1 .
    CCDSi CCDS24042.1.
    RefSeqi NP_073144.3. NM_022653.4.
    UniGenei Mm.26995.

    3D structure databases

    ProteinModelPortali Q8C1A5.
    SMRi Q8C1A5. Positions 24-677.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 206040. 2 interactions.
    IntActi Q8C1A5. 1 interaction.
    MINTi MINT-4128941.

    Protein family/group databases

    MEROPSi M03.001.

    PTM databases

    PhosphoSitei Q8C1A5.

    2D gel databases

    REPRODUCTION-2DPAGE Q8C1A5.

    Proteomic databases

    MaxQBi Q8C1A5.
    PaxDbi Q8C1A5.
    PRIDEi Q8C1A5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005057 ; ENSMUSP00000005057 ; ENSMUSG00000004929 .
    GeneIDi 50492.
    KEGGi mmu:50492.
    UCSCi uc007gfv.2. mouse.

    Organism-specific databases

    CTDi 7064.
    MGIi MGI:1354165. Thop1.

    Phylogenomic databases

    eggNOGi COG0339.
    GeneTreei ENSGT00550000074738.
    HOGENOMi HOG000245985.
    HOVERGENi HBG000238.
    InParanoidi Q8C1A5.
    KOi K01392.
    OrthoDBi EOG7SR4KW.
    PhylomeDBi Q8C1A5.
    TreeFami TF300459.

    Miscellaneous databases

    NextBioi 307472.
    PROi Q8C1A5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8C1A5.
    CleanExi MM_THOP1.
    Genevestigatori Q8C1A5.

    Family and domain databases

    Gene3Di 1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProi IPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view ]
    Pfami PF01432. Peptidase_M3. 1 hit.
    [Graphical view ]
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The neuropeptide processing enzyme EC 3.4.24.15 is modulated by protein kinase A phosphorylation."
      Tullai J.W., Cummins P.M., Pabon A., Roberts J.L., Lopingco M.C., Shrimpton C.N., Smith A.I., Martignetti J.A., Ferro E.S., Glucksman M.J.
      J. Biol. Chem. 275:36514-36522(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Skin.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II and FVB/N.
      Tissue: Colon and Mammary tumor.
    4. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-278, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiTHOP1_MOUSE
    AccessioniPrimary (citable) accession number: Q8C1A5
    Secondary accession number(s): Q8K0J9, Q8K2D4, Q9EPX1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3