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Protein

Thimet oligopeptidase

Gene

Thop1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the beta-amyloid precursor protein and generate amyloidogenic fragments (By similarity).By similarity

Catalytic activityi

Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi473Zinc; catalyticPROSITE-ProRule annotation1
Active sitei474PROSITE-ProRule annotation1
Metal bindingi477Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi480Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: MGI

GO - Biological processi

  • intracellular signal transduction Source: MGI
  • peptide metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM03.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Thimet oligopeptidase (EC:3.4.24.15)
Gene namesi
Name:Thop1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1354165. Thop1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003190441 – 687Thimet oligopeptidaseAdd BLAST687

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16PhosphoserineBy similarity1
Modified residuei172PhosphoserineCombined sources1
Modified residuei257N6-acetyllysineBy similarity1
Modified residuei278PhosphotyrosineCombined sources1
Modified residuei538N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8C1A5.
MaxQBiQ8C1A5.
PaxDbiQ8C1A5.
PeptideAtlasiQ8C1A5.
PRIDEiQ8C1A5.

2D gel databases

REPRODUCTION-2DPAGEQ8C1A5.

PTM databases

iPTMnetiQ8C1A5.
PhosphoSitePlusiQ8C1A5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004929.
CleanExiMM_THOP1.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi206040. 2 interactors.
IntActiQ8C1A5. 1 interactor.
MINTiMINT-4128941.
STRINGi10090.ENSMUSP00000005057.

Structurei

3D structure databases

ProteinModelPortaliQ8C1A5.
SMRiQ8C1A5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Phylogenomic databases

eggNOGiKOG2089. Eukaryota.
COG0339. LUCA.
HOGENOMiHOG000245985.
HOVERGENiHBG000238.
InParanoidiQ8C1A5.
KOiK01392.
PhylomeDBiQ8C1A5.
TreeFamiTF300459.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C1A5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPPAACAGD VVDAASPAST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR
60 70 80 90 100
VGAQNFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP CKDIRAASTE
110 120 130 140 150
ADKKLSEFDV EMSMRQDVYQ RVVWLQEKTP KNSLKPEAAR YLERLIKLGR
160 170 180 190 200
RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK NLNEDTTFLP FTREELGGLP
210 220 230 240 250
EDFLSSLEKA EDGKLKVTLK YPHYFPLLKK CHVPETRRLL EEAFNCRCKE
260 270 280 290 300
ENCAILKELV SLRAQKSSLL GFHTHADYVL EMNMAKTSQT VATFLDELAQ
310 320 330 340 350
KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR
360 370 380 390 400
VDQNLLKEYF PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR
410 420 430 440 450
DAASGEEIGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQLAVAAMVA
460 470 480 490 500
NFTKPTPDAP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF
510 520 530 540 550
VEAPSQMLEN WVWEKEPLMR MSQHYRTGSE APQDLLEKLI KSRQANAGLF
560 570 580 590 600
NLRQIVLAKV DQVLHTQTDA DPAEEYARLC QEILGVPATP GTNMPATFGH
610 620 630 640 650
LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG
660 670 680
SEDASAMLKQ FLGRDPKQDA FLLSKGLQVE GSEAPAC
Length:687
Mass (Da):78,026
Last modified:March 1, 2003 - v1
Checksum:i362D899CE5101662
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132N → D in AAG35061 (PubMed:10969067).Curated1
Sequence conflicti132N → D in AAH31722 (PubMed:15489334).Curated1
Sequence conflicti193 – 194RE → KK in AAG35061 (PubMed:10969067).Curated2
Sequence conflicti205S → I in AAG35061 (PubMed:10969067).Curated1
Sequence conflicti529S → G in AAG35061 (PubMed:10969067).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314187 mRNA. Translation: AAG35061.1.
AK028609 mRNA. Translation: BAC26031.1.
BC031175 mRNA. Translation: AAH31175.1.
BC031722 mRNA. Translation: AAH31722.1.
CCDSiCCDS24042.1.
RefSeqiNP_073144.3. NM_022653.4.
UniGeneiMm.26995.

Genome annotation databases

GeneIDi50492.
KEGGimmu:50492.
UCSCiuc007gfv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF314187 mRNA. Translation: AAG35061.1.
AK028609 mRNA. Translation: BAC26031.1.
BC031175 mRNA. Translation: AAH31175.1.
BC031722 mRNA. Translation: AAH31722.1.
CCDSiCCDS24042.1.
RefSeqiNP_073144.3. NM_022653.4.
UniGeneiMm.26995.

3D structure databases

ProteinModelPortaliQ8C1A5.
SMRiQ8C1A5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206040. 2 interactors.
IntActiQ8C1A5. 1 interactor.
MINTiMINT-4128941.
STRINGi10090.ENSMUSP00000005057.

Protein family/group databases

MEROPSiM03.001.

PTM databases

iPTMnetiQ8C1A5.
PhosphoSitePlusiQ8C1A5.

2D gel databases

REPRODUCTION-2DPAGEQ8C1A5.

Proteomic databases

EPDiQ8C1A5.
MaxQBiQ8C1A5.
PaxDbiQ8C1A5.
PeptideAtlasiQ8C1A5.
PRIDEiQ8C1A5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi50492.
KEGGimmu:50492.
UCSCiuc007gfv.2. mouse.

Organism-specific databases

CTDi7064.
MGIiMGI:1354165. Thop1.

Phylogenomic databases

eggNOGiKOG2089. Eukaryota.
COG0339. LUCA.
HOGENOMiHOG000245985.
HOVERGENiHBG000238.
InParanoidiQ8C1A5.
KOiK01392.
PhylomeDBiQ8C1A5.
TreeFamiTF300459.

Miscellaneous databases

PROiQ8C1A5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004929.
CleanExiMM_THOP1.

Family and domain databases

Gene3Di1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHOP1_MOUSE
AccessioniPrimary (citable) accession number: Q8C1A5
Secondary accession number(s): Q8K0J9, Q8K2D4, Q9EPX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.