ID   VP9D1_MOUSE             Reviewed;         649 AA.
AC   Q8C190; Q6PFD1;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   24-JAN-2024, entry version 124.
DE   RecName: Full=VPS9 domain-containing protein 1;
DE   AltName: Full=5-day ovary-specific transcript 1 protein;
GN   Name=Vps9d1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   STRAIN=ICR; TISSUE=Ovary;
RA   Park C.E., Hwang K.C., Kim N.H., Lee K.A.;
RT   "Identification of differentially expressed transcripts between 1 day ovary
RT   and 5 day ovary.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J; TISSUE=Skin;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=C57BL/6J; TISSUE=Embryonic brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-116, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, and Kidney;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q8C190-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q8C190-2; Sequence=VSP_014703, VSP_014704;
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DR   EMBL; AY751521; AAV30827.1; -; mRNA.
DR   EMBL; AK028751; BAC26098.1; -; mRNA.
DR   EMBL; BC057621; AAH57621.1; -; mRNA.
DR   CCDS; CCDS52698.1; -. [Q8C190-1]
DR   RefSeq; NP_001297590.1; NM_001310661.1.
DR   RefSeq; NP_082476.1; NM_028200.2. [Q8C190-1]
DR   AlphaFoldDB; Q8C190; -.
DR   SMR; Q8C190; -.
DR   BioGRID; 215310; 2.
DR   STRING; 10090.ENSMUSP00000113634; -.
DR   iPTMnet; Q8C190; -.
DR   PhosphoSitePlus; Q8C190; -.
DR   EPD; Q8C190; -.
DR   MaxQB; Q8C190; -.
DR   PaxDb; 10090-ENSMUSP00000113575; -.
DR   ProteomicsDB; 297929; -. [Q8C190-1]
DR   ProteomicsDB; 297930; -. [Q8C190-2]
DR   Pumba; Q8C190; -.
DR   Antibodypedia; 1753; 48 antibodies from 8 providers.
DR   Ensembl; ENSMUST00000117643.9; ENSMUSP00000113748.3; ENSMUSG00000001062.19. [Q8C190-2]
DR   Ensembl; ENSMUST00000122363.8; ENSMUSP00000113575.2; ENSMUSG00000001062.19. [Q8C190-1]
DR   GeneID; 72325; -.
DR   KEGG; mmu:72325; -.
DR   UCSC; uc009nuv.2; mouse. [Q8C190-1]
DR   AGR; MGI:1914143; -.
DR   CTD; 9605; -.
DR   MGI; MGI:1914143; Vps9d1.
DR   VEuPathDB; HostDB:ENSMUSG00000001062; -.
DR   eggNOG; KOG2319; Eukaryota.
DR   GeneTree; ENSGT00390000015057; -.
DR   HOGENOM; CLU_017849_0_0_1; -.
DR   InParanoid; Q8C190; -.
DR   OMA; RVICECA; -.
DR   OrthoDB; 5478325at2759; -.
DR   PhylomeDB; Q8C190; -.
DR   TreeFam; TF332037; -.
DR   BioGRID-ORCS; 72325; 3 hits in 76 CRISPR screens.
DR   PRO; PR:Q8C190; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; Q8C190; Protein.
DR   Bgee; ENSMUSG00000001062; Expressed in saccule of membranous labyrinth and 259 other cell types or tissues.
DR   ExpressionAtlas; Q8C190; baseline and differential.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0030139; C:endocytic vesicle; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0031267; F:small GTPase binding; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR   Gene3D; 1.20.1050.80; VPS9 domain; 1.
DR   InterPro; IPR036181; MIT_dom_sf.
DR   InterPro; IPR003123; VPS9.
DR   InterPro; IPR045046; Vps9-like.
DR   InterPro; IPR037191; VPS9_dom_sf.
DR   PANTHER; PTHR23101; RAB GDP/GTP EXCHANGE FACTOR; 1.
DR   PANTHER; PTHR23101:SF98; VPS9 DOMAIN-CONTAINING PROTEIN 1; 1.
DR   Pfam; PF02204; VPS9; 1.
DR   SMART; SM00167; VPS9; 1.
DR   SUPFAM; SSF116846; MIT domain; 1.
DR   SUPFAM; SSF109993; VPS9 domain; 1.
DR   PROSITE; PS51205; VPS9; 1.
DR   Genevisible; Q8C190; MM.
PE   1: Evidence at protein level;
KW   Alternative splicing; Phosphoprotein; Reference proteome.
FT   CHAIN           1..649
FT                   /note="VPS9 domain-containing protein 1"
FT                   /id="PRO_0000079280"
FT   DOMAIN          487..648
FT                   /note="VPS9"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00550"
FT   REGION          348..393
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        353..367
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         116
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   VAR_SEQ         552..555
FT                   /note="VRTL -> GAGI (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_014703"
FT   VAR_SEQ         556..649
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_014704"
SQ   SEQUENCE   649 AA;  71284 MW;  34385AD36C050552 CRC64;
     MAAAAGDGGA KPLQSAMKLA NKAIELDTGN RPREAYVEYL RSIHYISQVL LEDVENTTEA
     GETVPPETSK MLKLAEQCLE RAQSTATKLG RICLKPAVPA APPTPLPTSR HRRVCSDEGG
     KLSPFLPPEI FQKLQVAESQ NSKKELTPLE KASLQNQKLR ATYEARMARL DPSQAMQKTS
     LTLSLQRQMM ENLVIAKARE ETLQRKMEER RLRLQEAANR RFCSQVALTP EEREQRALYA
     AILEYEQDHD WPKHWRAKLK RSPGDLSLVT SLLSHLLSLP DHPISQLLKK LQCAVYSALY
     PIVSRAATSA APAPGSCSLP PEANGLLAPG SRRLRSSQSL YCMLSPSEPS AAPRVLDGPP
     PTPSVPTLHS GPLDREADGS PAGPSSPLVD ALSHLPDKDS SFEDLEQFLA MSEPQTPGAK
     REPLLECLKS TVKDIHNAID RLLSLTLLAF ESLNTATSKD RCLACIEEPF FSPLWPLLLA
     LYRSVHRTRE AAVSRSMELY RNAPPTALGI PTKLLPRVPE AQASTYPYCT AAQELGLLVL
     ESCPQKKLEC IVRTLRVICI CAEDYCRAQE ARPEGESQPP AAAIGADDLL PILSFVVLRS
     GLPQLVSECA ALEEFTHEGY LIGEEGYCLT SLQSALSYVE LLSRGRLDK
//