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Protein

Copine-1

Gene

Cpne1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner. Exhibits calcium-dependent phospholipid binding properties. Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner. May recruit target proteins to the cell membrane in a calcium-dependent manner. May function in membrane trafficking. Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit. Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1483206. Glycerophospholipid biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Copine-1Curated
Alternative name(s):
Copine IBy similarityImported
Gene namesi
Name:Cpne1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2386621. Cpne1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Translocates to the cell membrane in a calcium-dependent manner.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 536536Copine-1PRO_0000144835Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei170 – 1701N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8C166.
MaxQBiQ8C166.
PaxDbiQ8C166.
PRIDEiQ8C166.

2D gel databases

REPRODUCTION-2DPAGEQ8C166.

PTM databases

iPTMnetiQ8C166.
PhosphoSiteiQ8C166.
SwissPalmiQ8C166.

Expressioni

Gene expression databases

BgeeiQ8C166.
ExpressionAtlasiQ8C166. baseline and differential.
GenevisibleiQ8C166. MM.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its C2 domains. Interacts with p65/RELA (via N-terminus); this interaction induces proteolytic cleavage of p65/RELA subunit and inhibition of NF-kappa-B transcriptional activity. Interacts (via VWFA domain) with ACTB, CCDC22, MYCBP2, PPP5C, RDX and UBE2O.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234468. 2 interactions.
IntActiQ8C166. 1 interaction.
MINTiMINT-4091849.
STRINGi10090.ENSMUSP00000105236.

Structurei

3D structure databases

ProteinModelPortaliQ8C166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9797C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini143 – 22785C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini284 – 504221VWFAPROSITE-ProRule annotationAdd
BLAST

Domaini

C2 domains are necessary for calcium-dependent cell membrane association. C2 domains are necessary for neuronal progenitor cell differentiation in a calcium-independent manner.By similarity

Sequence similaritiesi

Belongs to the copine family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1327. Eukaryota.
ENOG410XPC8. LUCA.
GeneTreeiENSGT00760000119085.
HOGENOMiHOG000220898.
HOVERGENiHBG066841.
InParanoidiQ8C166.
OMAiQEMKDSR.
PhylomeDBiQ8C166.
TreeFamiTF316419.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR010734. Copine.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF07002. Copine. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF53300. SSF53300. 1 hit.
PROSITEiPS50004. C2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHCVTLVQL SVSCEHLIDK DIGSKSDPLC VLLQDVGGAW AELCRTERVR
60 70 80 90 100
NCSSPEFSKT LQIEYHFETV QKLRFGIYDI DNKTPELGDD DFLGGAECSL
110 120 130 140 150
GQIVSSQTLT LPLMLKPGKP AGRGTITVSA QELKDSRVVT MEVEARNLDK
160 170 180 190 200
KDFLGKSDPF LEFFRQGDGK WQLAYRTEVV KNNLNPTWKR FSVSLQHFCG
210 220 230 240 250
GDLSTPIQVR CSDYDSDGSH DLIGTFHTTL AQLQAVPAEF ECVHPEKQQR
260 270 280 290 300
KKNYRNSGTV RVKTCRVETE YSFLDYVMGG CQINFTVGVD FTGSNGDPSS
310 320 330 340 350
PDSLHYLSPT GVNEYLTALW SVGSVVQDYD SDKLFPAFGF GAQVPPDWQV
360 370 380 390 400
SHEFALNFNP SNPYCAGIQG IVDAYRQALP QVRLYGPTNF APIINHVARF
410 420 430 440 450
AAQAAQQRSA SQYFVLLLLT DGAVTDVEAT CKAVVDASKL PMSVIIVGVG
460 470 480 490 500
GADFEVMEQL DADGGPLRTR SGEAAARDIV QFVPYRRFQN APRETLAQTV
510 520 530
LAEVPTQMVS YFRAQGWAPL KAPPTPGKGP AQAPQA
Length:536
Mass (Da):58,887
Last modified:March 1, 2003 - v1
Checksum:iDF603A71A1A74F7B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431L → P in AAH57554 (PubMed:15489334).Curated
Sequence conflicti452 – 4543ADF → GHS in AAK56087 (PubMed:11471062).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028882 mRNA. Translation: BAC26170.1.
BC057554 mRNA. Translation: AAH57554.1.
AF332057 mRNA. Translation: AAK56086.1.
AF332058 mRNA. Translation: AAK56087.1.
CCDSiCCDS16960.1.
RefSeqiNP_733467.1. NM_170588.3.
NP_733469.1. NM_170590.3.
UniGeneiMm.27660.

Genome annotation databases

EnsembliENSMUST00000109607; ENSMUSP00000105236; ENSMUSG00000074643.
ENSMUST00000109608; ENSMUSP00000105237; ENSMUSG00000074643.
GeneIDi266692.
KEGGimmu:266692.
UCSCiuc008nmf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028882 mRNA. Translation: BAC26170.1.
BC057554 mRNA. Translation: AAH57554.1.
AF332057 mRNA. Translation: AAK56086.1.
AF332058 mRNA. Translation: AAK56087.1.
CCDSiCCDS16960.1.
RefSeqiNP_733467.1. NM_170588.3.
NP_733469.1. NM_170590.3.
UniGeneiMm.27660.

3D structure databases

ProteinModelPortaliQ8C166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234468. 2 interactions.
IntActiQ8C166. 1 interaction.
MINTiMINT-4091849.
STRINGi10090.ENSMUSP00000105236.

PTM databases

iPTMnetiQ8C166.
PhosphoSiteiQ8C166.
SwissPalmiQ8C166.

2D gel databases

REPRODUCTION-2DPAGEQ8C166.

Proteomic databases

EPDiQ8C166.
MaxQBiQ8C166.
PaxDbiQ8C166.
PRIDEiQ8C166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109607; ENSMUSP00000105236; ENSMUSG00000074643.
ENSMUST00000109608; ENSMUSP00000105237; ENSMUSG00000074643.
GeneIDi266692.
KEGGimmu:266692.
UCSCiuc008nmf.2. mouse.

Organism-specific databases

CTDi8904.
MGIiMGI:2386621. Cpne1.

Phylogenomic databases

eggNOGiKOG1327. Eukaryota.
ENOG410XPC8. LUCA.
GeneTreeiENSGT00760000119085.
HOGENOMiHOG000220898.
HOVERGENiHBG066841.
InParanoidiQ8C166.
OMAiQEMKDSR.
PhylomeDBiQ8C166.
TreeFamiTF316419.

Enzyme and pathway databases

ReactomeiR-MMU-1483206. Glycerophospholipid biosynthesis.

Miscellaneous databases

NextBioi392184.
PROiQ8C166.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C166.
ExpressionAtlasiQ8C166. baseline and differential.
GenevisibleiQ8C166. MM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR010734. Copine.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF07002. Copine. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF53300. SSF53300. 1 hit.
PROSITEiPS50004. C2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
    Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
    Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-454.
    Strain: ILS and ISS.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen and Testis.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiCPNE1_MOUSE
AccessioniPrimary (citable) accession number: Q8C166
Secondary accession number(s): Q925K4, Q925K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: March 1, 2003
Last modified: March 16, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.