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Q8C147

- DOCK8_MOUSE

UniProt

Q8C147 - DOCK8_MOUSE

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Protein

Dedicator of cytokinesis protein 8

Gene

Dock8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP (By similarity).By similarity

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: MGI

GO - Biological processi

  1. dendritic cell migration Source: MGI
  2. immunological synapse formation Source: MGI
  3. memory T cell proliferation Source: Ensembl
  4. negative regulation of T cell apoptotic process Source: MGI
  5. positive regulation of GTPase activity Source: GOC
  6. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 8
Gene namesi
Name:Dock8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1921396. Dock8.

Subcellular locationi

GO - Cellular componenti

  1. cell leading edge Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21002100Dedicator of cytokinesis protein 8PRO_0000189998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei452 – 4521PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8C147.
PaxDbiQ8C147.
PRIDEiQ8C147.

PTM databases

PhosphoSiteiQ8C147.

Expressioni

Gene expression databases

BgeeiQ8C147.
CleanExiMM_DOCK8.
ExpressionAtlasiQ8C147. baseline and differential.
GenevestigatoriQ8C147.

Interactioni

Protein-protein interaction databases

BioGridi217953. 1 interaction.

Structurei

Secondary structure

1
2100
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1794 – 18029
Helixi1803 – 18086
Beta strandi1812 – 18209
Helixi1823 – 183816
Helixi1840 – 18423
Beta strandi1843 – 18464
Helixi1854 – 18563
Beta strandi1861 – 187111
Helixi1875 – 18784
Beta strandi1891 – 190010
Beta strandi1904 – 19063
Helixi1910 – 19123
Beta strandi1914 – 192714
Beta strandi1929 – 194315
Helixi1945 – 196521
Helixi1971 – 198212
Beta strandi1985 – 19873
Helixi1990 – 19978
Helixi2005 – 203531
Helixi2038 – 20403
Helixi2041 – 206222

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VHLX-ray2.08A1787-2067[»]
ProteinModelPortaliQ8C147.
SMRiQ8C147. Positions 1637-2066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini561 – 730170DHR-1Add
BLAST
Domaini1633 – 2067435DHR-2Add
BLAST

Domaini

The DHR-2 domain may mediate some GEF activity.By similarity

Sequence similaritiesi

Belongs to the DOCK family.Curated
Contains 1 DHR-1 domain.Curated
Contains 1 DHR-2 domain.Curated

Phylogenomic databases

eggNOGiNOG242127.
GeneTreeiENSGT00760000119234.
HOVERGENiHBG051390.
InParanoidiQ8C147.
OMAiQDNHLEK.
OrthoDBiEOG7P8P7G.
PhylomeDBiQ8C147.
TreeFamiTF313629.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 1 hit.
PfamiPF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8C147) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATLPSAERR AFALKINRYS SSEIRKQFTL PPNLGQYHRH SISTSGFPSL
60 70 80 90 100
QLPQLYEPVE PVDFEGLVMT HLNSLDAELA QELGDLTDDD LHVAFTPKEC
110 120 130 140 150
RTLQHSLPEE GVELDPHVRD CVQTYIREWL IVNRKNQGSS EFCSFKKTGS
160 170 180 190 200
RRDFQKTLQK QTFESETLEC SEPDTQTGPR HPLNVLCDVS GKGPLTSCDF
210 220 230 240 250
DLRSLQPDER LENLLQLVSA EDFEKEKEEA RKTNRPAELF ALYPPVDEED
260 270 280 290 300
AVEIRPVPEC PKEHLGNRIL VKVLTLKFEI EIEPLFASIA LYDVKERKKI
310 320 330 340 350
SENFHCDLNS DQFKGFLRAH TPSIDPSSQA RSAVFSVTYP SSDIYLVVKI
360 370 380 390 400
EKVLQQGEIA DCAEPYMIIK ESDGGKSKEK VEKLKLQAES FCQRLGKYRM
410 420 430 440 450
PFAWAPISLA SFFNISTLER ESTDVEPGVG RNSVGEKRSL SQSRRPSERT
460 470 480 490 500
LSLEENGVGS NFKATTLATN IFFKQEGDRL SDEDLFKFLA DYKRSSSLQR
510 520 530 540 550
RVKSIPGSLR LEISPAPDVM NCCLTPEMLP VKPFPENRTR PHKEILEFPI
560 570 580 590 600
REVYVPHTVY RNLLYVYPQR LNFASKLASA RNITIKIQFM CGEDPSNAMP
610 620 630 640 650
VIFGKSSGPE FLQEVYTAIT YHNKSPDFYE EVKIKLPAKL TVNHHLLFTF
660 670 680 690 700
YHISCQQKQG ASGESLLGYS WLPILLNERL QTGSYCLPVA LEKLPPNYSI
710 720 730 740 750
HSAEKVPLQN PPIKWAEGHK GVFNIEVQAV SSVHTQDNHL EKFFTLCHSL
760 770 780 790 800
ESQVSFPIRV LDQKITESTL EHELKLSIIC LNSSRLEPLV LFLHLVLDKL
810 820 830 840 850
FQLSVQPMVI AGQTANFSQF AFESVVAIAN SLHNSKDLRK DQHGRNCLLA
860 870 880 890 900
SYVHYVFRLP ELHRDTSKSG GPTTVVPDPR YHTYGRTSAA AVSSKLMQAR
910 920 930 940 950
VMSSSNPDLT GSHCAADEEV KNIMSSKIAD RNCSRMSYYC SGNSDAPGST
960 970 980 990 1000
AAPRPVSKKH FHEELALQMV VSTGVVRETV FKYAWFFFEL LVKSMAQYVH
1010 1020 1030 1040 1050
NLDKRDSFRR TRFSDRFKDD ITTIVNVVTS EIAALLVKPQ KESEQAEKIN
1060 1070 1080 1090 1100
ISLAFFLYDL LSIMDRGFVF NLIKHYCSQL SAKLNILPTL ISMRLEFLRI
1110 1120 1130 1140 1150
LCSHEHYLNL NLLFMNTDTA PASPCPSISS QNSSSCSSFQ DQKIASMFDL
1160 1170 1180 1190 1200
TPEYRQQHFL TGLLFTELAV ALDAEGDGIS RVQRKAVSAI HSLLCSHDLD
1210 1220 1230 1240 1250
PRCRKPEVKV KIAALYLPLV GIILDALPQL YDFTDARSGR SRASGSYEEQ
1260 1270 1280 1290 1300
DVANGINQNV ALAIAGNHFN LKTSGAMLSS LPYKQYNMLN ADTTRHLMIC
1310 1320 1330 1340 1350
FLWIMKNADQ SLIRKWIADL PSMQLNRILD LLFICVSCFE YKGKQSSDKV
1360 1370 1380 1390 1400
SNQVLQKSRD VKAKLEEALL RGEGARGEMM RRRIPGTDRF PGINENLRWR
1410 1420 1430 1440 1450
KEQTQWRQAN EKLDKTKAEL DQEALISGNL ATEANLIILD MQENIIQASS
1460 1470 1480 1490 1500
ALDCKDSLLG GVLRVLVNSL SCDQSTTYLT HCFATLRALI AKFGDLLFEE
1510 1520 1530 1540 1550
EMEQCADLCQ RVLHHCSSSM DVTRSQACAT LYLLMRFSFG ATSNFARVKM
1560 1570 1580 1590 1600
QVTMALASLV GKAPDFNEEH LRRSLRTILA YSEEDTAMQT TPFPMQVEEL
1610 1620 1630 1640 1650
LCNLNSILYD TVKMREFQED PEMLMDLMYR IAKSYQASPD LRLTWLQNMA
1660 1670 1680 1690 1700
EKHTKKKCFT EAAMCLVHAA ALVAEYLSML EDHSYLPVGS VSFQNISSNV
1710 1720 1730 1740 1750
LEESAVSDDT LSPDEDGVCS GRYFTESGLV GLLEQAAELF STGGLYETVN
1760 1770 1780 1790 1800
EVYKLVIPIL EAHRDFRKLT STHDKLQKAF DNIINKDHKR MFGTYFRVGF
1810 1820 1830 1840 1850
YGSRFGDLDE QEFVYKEPAI TKLPEISHRL EGFYGQCFGA EFVEVIKDST
1860 1870 1880 1890 1900
PVDKTKLDPN KAYIQITFVE PYFDEYEMKD RVTYFEKNFN LRRFMYTTPF
1910 1920 1930 1940 1950
TLEGRPRGEL HEQHRRNTVL TTMHAFPYIK TRIRVSQKEE FVLTPIEVAI
1960 1970 1980 1990 2000
EDMKKKTLQL AVATHQEPPD AKMLQMVLQG SVGATVNQGP LEVAQVFLAE
2010 2020 2030 2040 2050
IPADPKLYRH HNKLRLCFKE FIMRCGEAVE KNRRLITAEQ REYQQELKKN
2060 2070 2080 2090 2100
YNKLRDSLRP MIERKIPELY KPIFRVDSQK RDSFHRSSFR KCETQLSQGS
Length:2,100
Mass (Da):238,978
Last modified:August 21, 2007 - v4
Checksum:iE867E04339CC6009
GO
Isoform 2 (identifier: Q8C147-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-366: Missing.
     1042-1044: ESE → VAV
     1045-2100: Missing.

Note: No experimental confirmation available.

Show »
Length:678
Mass (Da):76,556
Checksum:i56753BFF16C1FB5F
GO

Sequence cautioni

The sequence AAH30316.2 differs from that shown. Reason: Erroneous initiation.
The sequence BAB23587.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC26219.2 differs from that shown. Reason: Erroneous initiation.
The sequence BAD21430.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1608 – 16081L → M in BAC26219. (PubMed:16141072)Curated
Sequence conflicti1616 – 16161E → K in AAH43470. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 366366Missing in isoform 2. 1 PublicationVSP_027374Add
BLAST
Alternative sequencei1042 – 10443ESE → VAV in isoform 2. 1 PublicationVSP_027375
Alternative sequencei1045 – 21001056Missing in isoform 2. 1 PublicationVSP_027376Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC139711 Genomic DNA. No translation available.
AC132319 Genomic DNA. No translation available.
AK004816 mRNA. Translation: BAB23587.1. Different initiation.
AK028968 mRNA. Translation: BAC26219.2. Different initiation.
AK038285 mRNA. Translation: BAC29959.1.
BC029018 mRNA. Translation: AAH29018.2.
BC030316 mRNA. Translation: AAH30316.2. Different initiation.
BC043470 mRNA. Translation: AAH43470.1.
BC055295 mRNA. Translation: AAH55295.1.
AK131180 mRNA. Translation: BAD21430.1. Different initiation.
CCDSiCCDS37943.1. [Q8C147-1]
RefSeqiNP_083061.2. NM_028785.3. [Q8C147-1]
UniGeneiMm.102485.

Genome annotation databases

EnsembliENSMUST00000025831; ENSMUSP00000025831; ENSMUSG00000052085. [Q8C147-1]
GeneIDi76088.
KEGGimmu:76088.
UCSCiuc008hba.1. mouse. [Q8C147-1]
uc008hbc.1. mouse. [Q8C147-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC139711 Genomic DNA. No translation available.
AC132319 Genomic DNA. No translation available.
AK004816 mRNA. Translation: BAB23587.1 . Different initiation.
AK028968 mRNA. Translation: BAC26219.2 . Different initiation.
AK038285 mRNA. Translation: BAC29959.1 .
BC029018 mRNA. Translation: AAH29018.2 .
BC030316 mRNA. Translation: AAH30316.2 . Different initiation.
BC043470 mRNA. Translation: AAH43470.1 .
BC055295 mRNA. Translation: AAH55295.1 .
AK131180 mRNA. Translation: BAD21430.1 . Different initiation.
CCDSi CCDS37943.1. [Q8C147-1 ]
RefSeqi NP_083061.2. NM_028785.3. [Q8C147-1 ]
UniGenei Mm.102485.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VHL X-ray 2.08 A 1787-2067 [» ]
ProteinModelPortali Q8C147.
SMRi Q8C147. Positions 1637-2066.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 217953. 1 interaction.

PTM databases

PhosphoSitei Q8C147.

Proteomic databases

MaxQBi Q8C147.
PaxDbi Q8C147.
PRIDEi Q8C147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025831 ; ENSMUSP00000025831 ; ENSMUSG00000052085 . [Q8C147-1 ]
GeneIDi 76088.
KEGGi mmu:76088.
UCSCi uc008hba.1. mouse. [Q8C147-1 ]
uc008hbc.1. mouse. [Q8C147-2 ]

Organism-specific databases

CTDi 81704.
MGIi MGI:1921396. Dock8.

Phylogenomic databases

eggNOGi NOG242127.
GeneTreei ENSGT00760000119234.
HOVERGENi HBG051390.
InParanoidi Q8C147.
OMAi QDNHLEK.
OrthoDBi EOG7P8P7G.
PhylomeDBi Q8C147.
TreeFami TF313629.

Enzyme and pathway databases

Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

ChiTaRSi DOCK8. mouse.
NextBioi 344573.
PROi Q8C147.
SOURCEi Search...

Gene expression databases

Bgeei Q8C147.
CleanExi MM_DOCK8.
ExpressionAtlasi Q8C147. baseline and differential.
Genevestigatori Q8C147.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view ]
PANTHERi PTHR23317. PTHR23317. 1 hit.
Pfami PF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1581-2100 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Lung, Skin and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 967-2100 (ISOFORM 1).
    Strain: C57BL/6J, FVB/N and FVB/N-3.
    Tissue: Mammary gland.
  4. "Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
    DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 992-2100 (ISOFORM 1).
    Strain: ICR.
    Tissue: Embryonic tail.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDOCK8_MOUSE
AccessioniPrimary (citable) accession number: Q8C147
Secondary accession number(s): Q6KAM7
, Q6PIS0, Q7TMQ5, Q8CAP6, Q8K105, Q9DBQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: August 21, 2007
Last modified: October 29, 2014
This is version 87 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3