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Q8C147 (DOCK8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dedicator of cytokinesis protein 8
Gene names
Name:Dock8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length2100 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP By similarity.

Domain

The DHR-2 domain may mediate some GEF activity By similarity.

Sequence similarities

Belongs to the DOCK family.

Contains 1 DHR-1 domain.

Contains 1 DHR-2 domain.

Sequence caution

The sequence AAH30316.2 differs from that shown. Reason: Erroneous initiation.

The sequence BAB23587.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC26219.2 differs from that shown. Reason: Erroneous initiation.

The sequence BAD21430.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   Molecular functionGuanine-nucleotide releasing factor
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processsmall GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Molecular_functionguanyl-nucleotide exchange factor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8C147-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8C147-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-366: Missing.
     1042-1044: ESE → VAV
     1045-2100: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 21002100Dedicator of cytokinesis protein 8
PRO_0000189998

Regions

Domain561 – 730170DHR-1
Domain1633 – 2067435DHR-2

Amino acid modifications

Modified residue4521Phosphoserine By similarity

Natural variations

Alternative sequence1 – 366366Missing in isoform 2.
VSP_027374
Alternative sequence1042 – 10443ESE → VAV in isoform 2.
VSP_027375
Alternative sequence1045 – 21001056Missing in isoform 2.
VSP_027376

Experimental info

Sequence conflict16081L → M in BAC26219. Ref.2
Sequence conflict16161E → K in AAH43470. Ref.3

Secondary structure

........................................ 2100
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 21, 2007. Version 4.
Checksum: E867E04339CC6009

FASTA2,100238,978
        10         20         30         40         50         60 
MATLPSAERR AFALKINRYS SSEIRKQFTL PPNLGQYHRH SISTSGFPSL QLPQLYEPVE 

        70         80         90        100        110        120 
PVDFEGLVMT HLNSLDAELA QELGDLTDDD LHVAFTPKEC RTLQHSLPEE GVELDPHVRD 

       130        140        150        160        170        180 
CVQTYIREWL IVNRKNQGSS EFCSFKKTGS RRDFQKTLQK QTFESETLEC SEPDTQTGPR 

       190        200        210        220        230        240 
HPLNVLCDVS GKGPLTSCDF DLRSLQPDER LENLLQLVSA EDFEKEKEEA RKTNRPAELF 

       250        260        270        280        290        300 
ALYPPVDEED AVEIRPVPEC PKEHLGNRIL VKVLTLKFEI EIEPLFASIA LYDVKERKKI 

       310        320        330        340        350        360 
SENFHCDLNS DQFKGFLRAH TPSIDPSSQA RSAVFSVTYP SSDIYLVVKI EKVLQQGEIA 

       370        380        390        400        410        420 
DCAEPYMIIK ESDGGKSKEK VEKLKLQAES FCQRLGKYRM PFAWAPISLA SFFNISTLER 

       430        440        450        460        470        480 
ESTDVEPGVG RNSVGEKRSL SQSRRPSERT LSLEENGVGS NFKATTLATN IFFKQEGDRL 

       490        500        510        520        530        540 
SDEDLFKFLA DYKRSSSLQR RVKSIPGSLR LEISPAPDVM NCCLTPEMLP VKPFPENRTR 

       550        560        570        580        590        600 
PHKEILEFPI REVYVPHTVY RNLLYVYPQR LNFASKLASA RNITIKIQFM CGEDPSNAMP 

       610        620        630        640        650        660 
VIFGKSSGPE FLQEVYTAIT YHNKSPDFYE EVKIKLPAKL TVNHHLLFTF YHISCQQKQG 

       670        680        690        700        710        720 
ASGESLLGYS WLPILLNERL QTGSYCLPVA LEKLPPNYSI HSAEKVPLQN PPIKWAEGHK 

       730        740        750        760        770        780 
GVFNIEVQAV SSVHTQDNHL EKFFTLCHSL ESQVSFPIRV LDQKITESTL EHELKLSIIC 

       790        800        810        820        830        840 
LNSSRLEPLV LFLHLVLDKL FQLSVQPMVI AGQTANFSQF AFESVVAIAN SLHNSKDLRK 

       850        860        870        880        890        900 
DQHGRNCLLA SYVHYVFRLP ELHRDTSKSG GPTTVVPDPR YHTYGRTSAA AVSSKLMQAR 

       910        920        930        940        950        960 
VMSSSNPDLT GSHCAADEEV KNIMSSKIAD RNCSRMSYYC SGNSDAPGST AAPRPVSKKH 

       970        980        990       1000       1010       1020 
FHEELALQMV VSTGVVRETV FKYAWFFFEL LVKSMAQYVH NLDKRDSFRR TRFSDRFKDD 

      1030       1040       1050       1060       1070       1080 
ITTIVNVVTS EIAALLVKPQ KESEQAEKIN ISLAFFLYDL LSIMDRGFVF NLIKHYCSQL 

      1090       1100       1110       1120       1130       1140 
SAKLNILPTL ISMRLEFLRI LCSHEHYLNL NLLFMNTDTA PASPCPSISS QNSSSCSSFQ 

      1150       1160       1170       1180       1190       1200 
DQKIASMFDL TPEYRQQHFL TGLLFTELAV ALDAEGDGIS RVQRKAVSAI HSLLCSHDLD 

      1210       1220       1230       1240       1250       1260 
PRCRKPEVKV KIAALYLPLV GIILDALPQL YDFTDARSGR SRASGSYEEQ DVANGINQNV 

      1270       1280       1290       1300       1310       1320 
ALAIAGNHFN LKTSGAMLSS LPYKQYNMLN ADTTRHLMIC FLWIMKNADQ SLIRKWIADL 

      1330       1340       1350       1360       1370       1380 
PSMQLNRILD LLFICVSCFE YKGKQSSDKV SNQVLQKSRD VKAKLEEALL RGEGARGEMM 

      1390       1400       1410       1420       1430       1440 
RRRIPGTDRF PGINENLRWR KEQTQWRQAN EKLDKTKAEL DQEALISGNL ATEANLIILD 

      1450       1460       1470       1480       1490       1500 
MQENIIQASS ALDCKDSLLG GVLRVLVNSL SCDQSTTYLT HCFATLRALI AKFGDLLFEE 

      1510       1520       1530       1540       1550       1560 
EMEQCADLCQ RVLHHCSSSM DVTRSQACAT LYLLMRFSFG ATSNFARVKM QVTMALASLV 

      1570       1580       1590       1600       1610       1620 
GKAPDFNEEH LRRSLRTILA YSEEDTAMQT TPFPMQVEEL LCNLNSILYD TVKMREFQED 

      1630       1640       1650       1660       1670       1680 
PEMLMDLMYR IAKSYQASPD LRLTWLQNMA EKHTKKKCFT EAAMCLVHAA ALVAEYLSML 

      1690       1700       1710       1720       1730       1740 
EDHSYLPVGS VSFQNISSNV LEESAVSDDT LSPDEDGVCS GRYFTESGLV GLLEQAAELF 

      1750       1760       1770       1780       1790       1800 
STGGLYETVN EVYKLVIPIL EAHRDFRKLT STHDKLQKAF DNIINKDHKR MFGTYFRVGF 

      1810       1820       1830       1840       1850       1860 
YGSRFGDLDE QEFVYKEPAI TKLPEISHRL EGFYGQCFGA EFVEVIKDST PVDKTKLDPN 

      1870       1880       1890       1900       1910       1920 
KAYIQITFVE PYFDEYEMKD RVTYFEKNFN LRRFMYTTPF TLEGRPRGEL HEQHRRNTVL 

      1930       1940       1950       1960       1970       1980 
TTMHAFPYIK TRIRVSQKEE FVLTPIEVAI EDMKKKTLQL AVATHQEPPD AKMLQMVLQG 

      1990       2000       2010       2020       2030       2040 
SVGATVNQGP LEVAQVFLAE IPADPKLYRH HNKLRLCFKE FIMRCGEAVE KNRRLITAEQ 

      2050       2060       2070       2080       2090       2100 
REYQQELKKN YNKLRDSLRP MIERKIPELY KPIFRVDSQK RDSFHRSSFR KCETQLSQGS 

« Hide

Isoform 2 [UniParc].

Checksum: 56753BFF16C1FB5F
Show »

FASTA67876,556

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1581-2100 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Lung, Skin and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 967-2100 (ISOFORM 1).
Strain: C57BL/6J, FVB/N and FVB/N-3.
Tissue: Mammary gland.
[4]"Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 992-2100 (ISOFORM 1).
Strain: ICR.
Tissue: Embryonic tail.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC139711 Genomic DNA. No translation available.
AC132319 Genomic DNA. No translation available.
AK004816 mRNA. Translation: BAB23587.1. Different initiation.
AK028968 mRNA. Translation: BAC26219.2. Different initiation.
AK038285 mRNA. Translation: BAC29959.1.
BC029018 mRNA. Translation: AAH29018.2.
BC030316 mRNA. Translation: AAH30316.2. Different initiation.
BC043470 mRNA. Translation: AAH43470.1.
BC055295 mRNA. Translation: AAH55295.1.
AK131180 mRNA. Translation: BAD21430.1. Different initiation.
RefSeqNP_083061.2. NM_028785.3.
UniGeneMm.102485.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VHLX-ray2.08A1787-2067[»]
ProteinModelPortalQ8C147.
SMRQ8C147. Positions 561-730, 1637-2066.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid217953. 1 interaction.

PTM databases

PhosphoSiteQ8C147.

Proteomic databases

PaxDbQ8C147.
PRIDEQ8C147.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025831; ENSMUSP00000025831; ENSMUSG00000052085. [Q8C147-1]
GeneID76088.
KEGGmmu:76088.
UCSCuc008hba.1. mouse. [Q8C147-1]
uc008hbc.1. mouse. [Q8C147-2]

Organism-specific databases

CTD81704.
MGIMGI:1921396. Dock8.

Phylogenomic databases

eggNOGNOG242127.
GeneTreeENSGT00740000115071.
HOVERGENHBG051390.
InParanoidQ8C147.
OMASVHTQDN.
OrthoDBEOG7P8P7G.
PhylomeDBQ8C147.
TreeFamTF313629.

Gene expression databases

ArrayExpressQ8C147.
BgeeQ8C147.
CleanExMM_DOCK8.
GenevestigatorQ8C147.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR026798. DOCK6/DOCK8.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view]
PANTHERPTHR23317. PTHR23317. 1 hit.
PTHR23317:SF27. PTHR23317:SF27. 1 hit.
PfamPF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDOCK8. mouse.
NextBio344573.
PROQ8C147.
SOURCESearch...

Entry information

Entry nameDOCK8_MOUSE
AccessionPrimary (citable) accession number: Q8C147
Secondary accession number(s): Q6KAM7 expand/collapse secondary AC list , Q6PIS0, Q7TMQ5, Q8CAP6, Q8K105, Q9DBQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: August 21, 2007
Last modified: April 16, 2014
This is version 81 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot