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Q8C147

- DOCK8_MOUSE

UniProt

Q8C147 - DOCK8_MOUSE

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Protein
Dedicator of cytokinesis protein 8
Gene
Dock8
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP By similarity.

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: MGI

GO - Biological processi

  1. dendritic cell migration Source: MGI
  2. immunological synapse formation Source: MGI
  3. negative regulation of T cell apoptotic process Source: MGI
  4. regulation of GTPase activity Source: GOC
  5. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 8
Gene namesi
Name:Dock8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1921396. Dock8.

Subcellular locationi

GO - Cellular componenti

  1. cell leading edge Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21002100Dedicator of cytokinesis protein 8
PRO_0000189998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei452 – 4521Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8C147.
PaxDbiQ8C147.
PRIDEiQ8C147.

PTM databases

PhosphoSiteiQ8C147.

Expressioni

Gene expression databases

ArrayExpressiQ8C147.
BgeeiQ8C147.
CleanExiMM_DOCK8.
GenevestigatoriQ8C147.

Interactioni

Protein-protein interaction databases

BioGridi217953. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1794 – 18029
Helixi1803 – 18086
Beta strandi1812 – 18209
Helixi1823 – 183816
Helixi1840 – 18423
Beta strandi1843 – 18464
Helixi1854 – 18563
Beta strandi1861 – 187111
Helixi1875 – 18784
Beta strandi1891 – 190010
Beta strandi1904 – 19063
Helixi1910 – 19123
Beta strandi1914 – 192714
Beta strandi1929 – 194315
Helixi1945 – 196521
Helixi1971 – 198212
Beta strandi1985 – 19873
Helixi1990 – 19978
Helixi2005 – 203531
Helixi2038 – 20403
Helixi2041 – 206222

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VHLX-ray2.08A1787-2067[»]
ProteinModelPortaliQ8C147.
SMRiQ8C147. Positions 1637-2066.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini561 – 730170DHR-1
Add
BLAST
Domaini1633 – 2067435DHR-2
Add
BLAST

Domaini

The DHR-2 domain may mediate some GEF activity By similarity.

Sequence similaritiesi

Belongs to the DOCK family.
Contains 1 DHR-1 domain.
Contains 1 DHR-2 domain.

Phylogenomic databases

eggNOGiNOG242127.
GeneTreeiENSGT00740000115071.
HOVERGENiHBG051390.
InParanoidiQ8C147.
OMAiQDNHLEK.
OrthoDBiEOG7P8P7G.
PhylomeDBiQ8C147.
TreeFamiTF313629.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 1 hit.
PfamiPF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8C147-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATLPSAERR AFALKINRYS SSEIRKQFTL PPNLGQYHRH SISTSGFPSL     50
QLPQLYEPVE PVDFEGLVMT HLNSLDAELA QELGDLTDDD LHVAFTPKEC 100
RTLQHSLPEE GVELDPHVRD CVQTYIREWL IVNRKNQGSS EFCSFKKTGS 150
RRDFQKTLQK QTFESETLEC SEPDTQTGPR HPLNVLCDVS GKGPLTSCDF 200
DLRSLQPDER LENLLQLVSA EDFEKEKEEA RKTNRPAELF ALYPPVDEED 250
AVEIRPVPEC PKEHLGNRIL VKVLTLKFEI EIEPLFASIA LYDVKERKKI 300
SENFHCDLNS DQFKGFLRAH TPSIDPSSQA RSAVFSVTYP SSDIYLVVKI 350
EKVLQQGEIA DCAEPYMIIK ESDGGKSKEK VEKLKLQAES FCQRLGKYRM 400
PFAWAPISLA SFFNISTLER ESTDVEPGVG RNSVGEKRSL SQSRRPSERT 450
LSLEENGVGS NFKATTLATN IFFKQEGDRL SDEDLFKFLA DYKRSSSLQR 500
RVKSIPGSLR LEISPAPDVM NCCLTPEMLP VKPFPENRTR PHKEILEFPI 550
REVYVPHTVY RNLLYVYPQR LNFASKLASA RNITIKIQFM CGEDPSNAMP 600
VIFGKSSGPE FLQEVYTAIT YHNKSPDFYE EVKIKLPAKL TVNHHLLFTF 650
YHISCQQKQG ASGESLLGYS WLPILLNERL QTGSYCLPVA LEKLPPNYSI 700
HSAEKVPLQN PPIKWAEGHK GVFNIEVQAV SSVHTQDNHL EKFFTLCHSL 750
ESQVSFPIRV LDQKITESTL EHELKLSIIC LNSSRLEPLV LFLHLVLDKL 800
FQLSVQPMVI AGQTANFSQF AFESVVAIAN SLHNSKDLRK DQHGRNCLLA 850
SYVHYVFRLP ELHRDTSKSG GPTTVVPDPR YHTYGRTSAA AVSSKLMQAR 900
VMSSSNPDLT GSHCAADEEV KNIMSSKIAD RNCSRMSYYC SGNSDAPGST 950
AAPRPVSKKH FHEELALQMV VSTGVVRETV FKYAWFFFEL LVKSMAQYVH 1000
NLDKRDSFRR TRFSDRFKDD ITTIVNVVTS EIAALLVKPQ KESEQAEKIN 1050
ISLAFFLYDL LSIMDRGFVF NLIKHYCSQL SAKLNILPTL ISMRLEFLRI 1100
LCSHEHYLNL NLLFMNTDTA PASPCPSISS QNSSSCSSFQ DQKIASMFDL 1150
TPEYRQQHFL TGLLFTELAV ALDAEGDGIS RVQRKAVSAI HSLLCSHDLD 1200
PRCRKPEVKV KIAALYLPLV GIILDALPQL YDFTDARSGR SRASGSYEEQ 1250
DVANGINQNV ALAIAGNHFN LKTSGAMLSS LPYKQYNMLN ADTTRHLMIC 1300
FLWIMKNADQ SLIRKWIADL PSMQLNRILD LLFICVSCFE YKGKQSSDKV 1350
SNQVLQKSRD VKAKLEEALL RGEGARGEMM RRRIPGTDRF PGINENLRWR 1400
KEQTQWRQAN EKLDKTKAEL DQEALISGNL ATEANLIILD MQENIIQASS 1450
ALDCKDSLLG GVLRVLVNSL SCDQSTTYLT HCFATLRALI AKFGDLLFEE 1500
EMEQCADLCQ RVLHHCSSSM DVTRSQACAT LYLLMRFSFG ATSNFARVKM 1550
QVTMALASLV GKAPDFNEEH LRRSLRTILA YSEEDTAMQT TPFPMQVEEL 1600
LCNLNSILYD TVKMREFQED PEMLMDLMYR IAKSYQASPD LRLTWLQNMA 1650
EKHTKKKCFT EAAMCLVHAA ALVAEYLSML EDHSYLPVGS VSFQNISSNV 1700
LEESAVSDDT LSPDEDGVCS GRYFTESGLV GLLEQAAELF STGGLYETVN 1750
EVYKLVIPIL EAHRDFRKLT STHDKLQKAF DNIINKDHKR MFGTYFRVGF 1800
YGSRFGDLDE QEFVYKEPAI TKLPEISHRL EGFYGQCFGA EFVEVIKDST 1850
PVDKTKLDPN KAYIQITFVE PYFDEYEMKD RVTYFEKNFN LRRFMYTTPF 1900
TLEGRPRGEL HEQHRRNTVL TTMHAFPYIK TRIRVSQKEE FVLTPIEVAI 1950
EDMKKKTLQL AVATHQEPPD AKMLQMVLQG SVGATVNQGP LEVAQVFLAE 2000
IPADPKLYRH HNKLRLCFKE FIMRCGEAVE KNRRLITAEQ REYQQELKKN 2050
YNKLRDSLRP MIERKIPELY KPIFRVDSQK RDSFHRSSFR KCETQLSQGS 2100
Length:2,100
Mass (Da):238,978
Last modified:August 21, 2007 - v4
Checksum:iE867E04339CC6009
GO
Isoform 2 (identifier: Q8C147-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-366: Missing.
     1042-1044: ESE → VAV
     1045-2100: Missing.

Note: No experimental confirmation available.

Show »
Length:678
Mass (Da):76,556
Checksum:i56753BFF16C1FB5F
GO

Sequence cautioni

The sequence AAH30316.2 differs from that shown. Reason: Erroneous initiation.
The sequence BAB23587.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC26219.2 differs from that shown. Reason: Erroneous initiation.
The sequence BAD21430.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 366366Missing in isoform 2.
VSP_027374Add
BLAST
Alternative sequencei1042 – 10443ESE → VAV in isoform 2.
VSP_027375
Alternative sequencei1045 – 21001056Missing in isoform 2.
VSP_027376Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1608 – 16081L → M in BAC26219. 1 Publication
Sequence conflicti1616 – 16161E → K in AAH43470. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC139711 Genomic DNA. No translation available.
AC132319 Genomic DNA. No translation available.
AK004816 mRNA. Translation: BAB23587.1. Different initiation.
AK028968 mRNA. Translation: BAC26219.2. Different initiation.
AK038285 mRNA. Translation: BAC29959.1.
BC029018 mRNA. Translation: AAH29018.2.
BC030316 mRNA. Translation: AAH30316.2. Different initiation.
BC043470 mRNA. Translation: AAH43470.1.
BC055295 mRNA. Translation: AAH55295.1.
AK131180 mRNA. Translation: BAD21430.1. Different initiation.
CCDSiCCDS37943.1. [Q8C147-1]
RefSeqiNP_083061.2. NM_028785.3. [Q8C147-1]
UniGeneiMm.102485.

Genome annotation databases

EnsembliENSMUST00000025831; ENSMUSP00000025831; ENSMUSG00000052085. [Q8C147-1]
GeneIDi76088.
KEGGimmu:76088.
UCSCiuc008hba.1. mouse. [Q8C147-1]
uc008hbc.1. mouse. [Q8C147-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC139711 Genomic DNA. No translation available.
AC132319 Genomic DNA. No translation available.
AK004816 mRNA. Translation: BAB23587.1 . Different initiation.
AK028968 mRNA. Translation: BAC26219.2 . Different initiation.
AK038285 mRNA. Translation: BAC29959.1 .
BC029018 mRNA. Translation: AAH29018.2 .
BC030316 mRNA. Translation: AAH30316.2 . Different initiation.
BC043470 mRNA. Translation: AAH43470.1 .
BC055295 mRNA. Translation: AAH55295.1 .
AK131180 mRNA. Translation: BAD21430.1 . Different initiation.
CCDSi CCDS37943.1. [Q8C147-1 ]
RefSeqi NP_083061.2. NM_028785.3. [Q8C147-1 ]
UniGenei Mm.102485.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VHL X-ray 2.08 A 1787-2067 [» ]
ProteinModelPortali Q8C147.
SMRi Q8C147. Positions 1637-2066.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 217953. 1 interaction.

PTM databases

PhosphoSitei Q8C147.

Proteomic databases

MaxQBi Q8C147.
PaxDbi Q8C147.
PRIDEi Q8C147.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025831 ; ENSMUSP00000025831 ; ENSMUSG00000052085 . [Q8C147-1 ]
GeneIDi 76088.
KEGGi mmu:76088.
UCSCi uc008hba.1. mouse. [Q8C147-1 ]
uc008hbc.1. mouse. [Q8C147-2 ]

Organism-specific databases

CTDi 81704.
MGIi MGI:1921396. Dock8.

Phylogenomic databases

eggNOGi NOG242127.
GeneTreei ENSGT00740000115071.
HOVERGENi HBG051390.
InParanoidi Q8C147.
OMAi QDNHLEK.
OrthoDBi EOG7P8P7G.
PhylomeDBi Q8C147.
TreeFami TF313629.

Enzyme and pathway databases

Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

ChiTaRSi DOCK8. mouse.
NextBioi 344573.
PROi Q8C147.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8C147.
Bgeei Q8C147.
CleanExi MM_DOCK8.
Genevestigatori Q8C147.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view ]
PANTHERi PTHR23317. PTHR23317. 1 hit.
Pfami PF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1581-2100 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Lung, Skin and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 967-2100 (ISOFORM 1).
    Strain: C57BL/6J, FVB/N and FVB/N-3.
    Tissue: Mammary gland.
  4. "Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
    DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 992-2100 (ISOFORM 1).
    Strain: ICR.
    Tissue: Embryonic tail.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDOCK8_MOUSE
AccessioniPrimary (citable) accession number: Q8C147
Secondary accession number(s): Q6KAM7
, Q6PIS0, Q7TMQ5, Q8CAP6, Q8K105, Q9DBQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: August 21, 2007
Last modified: September 3, 2014
This is version 85 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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