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Q8C147

- DOCK8_MOUSE

UniProt

Q8C147 - DOCK8_MOUSE

Protein

Dedicator of cytokinesis protein 8

Gene

Dock8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 4 (21 Aug 2007)
      Previous versions | rss
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    Functioni

    Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP By similarity.By similarity

    GO - Molecular functioni

    1. guanyl-nucleotide exchange factor activity Source: MGI

    GO - Biological processi

    1. dendritic cell migration Source: MGI
    2. immunological synapse formation Source: MGI
    3. negative regulation of T cell apoptotic process Source: MGI
    4. positive regulation of GTPase activity Source: GOC
    5. small GTPase mediated signal transduction Source: InterPro

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Enzyme and pathway databases

    ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dedicator of cytokinesis protein 8
    Gene namesi
    Name:Dock8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1921396. Dock8.

    Subcellular locationi

    GO - Cellular componenti

    1. cell leading edge Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 21002100Dedicator of cytokinesis protein 8PRO_0000189998Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei452 – 4521PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8C147.
    PaxDbiQ8C147.
    PRIDEiQ8C147.

    PTM databases

    PhosphoSiteiQ8C147.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8C147.
    BgeeiQ8C147.
    CleanExiMM_DOCK8.
    GenevestigatoriQ8C147.

    Interactioni

    Protein-protein interaction databases

    BioGridi217953. 1 interaction.

    Structurei

    Secondary structure

    1
    2100
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi1794 – 18029
    Helixi1803 – 18086
    Beta strandi1812 – 18209
    Helixi1823 – 183816
    Helixi1840 – 18423
    Beta strandi1843 – 18464
    Helixi1854 – 18563
    Beta strandi1861 – 187111
    Helixi1875 – 18784
    Beta strandi1891 – 190010
    Beta strandi1904 – 19063
    Helixi1910 – 19123
    Beta strandi1914 – 192714
    Beta strandi1929 – 194315
    Helixi1945 – 196521
    Helixi1971 – 198212
    Beta strandi1985 – 19873
    Helixi1990 – 19978
    Helixi2005 – 203531
    Helixi2038 – 20403
    Helixi2041 – 206222

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VHLX-ray2.08A1787-2067[»]
    ProteinModelPortaliQ8C147.
    SMRiQ8C147. Positions 1637-2066.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini561 – 730170DHR-1Add
    BLAST
    Domaini1633 – 2067435DHR-2Add
    BLAST

    Domaini

    The DHR-2 domain may mediate some GEF activity.By similarity

    Sequence similaritiesi

    Belongs to the DOCK family.Curated
    Contains 1 DHR-1 domain.Curated
    Contains 1 DHR-2 domain.Curated

    Phylogenomic databases

    eggNOGiNOG242127.
    GeneTreeiENSGT00740000115071.
    HOVERGENiHBG051390.
    InParanoidiQ8C147.
    OMAiQDNHLEK.
    OrthoDBiEOG7P8P7G.
    PhylomeDBiQ8C147.
    TreeFamiTF313629.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR027007. DHR-1_domain.
    IPR027357. DHR-2.
    IPR026791. DOCK.
    IPR010703. DOCK_C.
    IPR021816. DOCK_C/D_N.
    [Graphical view]
    PANTHERiPTHR23317. PTHR23317. 1 hit.
    PfamiPF06920. Ded_cyto. 1 hit.
    PF14429. DOCK-C2. 1 hit.
    PF11878. DUF3398. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS51650. DHR_1. 1 hit.
    PS51651. DHR_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8C147-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATLPSAERR AFALKINRYS SSEIRKQFTL PPNLGQYHRH SISTSGFPSL     50
    QLPQLYEPVE PVDFEGLVMT HLNSLDAELA QELGDLTDDD LHVAFTPKEC 100
    RTLQHSLPEE GVELDPHVRD CVQTYIREWL IVNRKNQGSS EFCSFKKTGS 150
    RRDFQKTLQK QTFESETLEC SEPDTQTGPR HPLNVLCDVS GKGPLTSCDF 200
    DLRSLQPDER LENLLQLVSA EDFEKEKEEA RKTNRPAELF ALYPPVDEED 250
    AVEIRPVPEC PKEHLGNRIL VKVLTLKFEI EIEPLFASIA LYDVKERKKI 300
    SENFHCDLNS DQFKGFLRAH TPSIDPSSQA RSAVFSVTYP SSDIYLVVKI 350
    EKVLQQGEIA DCAEPYMIIK ESDGGKSKEK VEKLKLQAES FCQRLGKYRM 400
    PFAWAPISLA SFFNISTLER ESTDVEPGVG RNSVGEKRSL SQSRRPSERT 450
    LSLEENGVGS NFKATTLATN IFFKQEGDRL SDEDLFKFLA DYKRSSSLQR 500
    RVKSIPGSLR LEISPAPDVM NCCLTPEMLP VKPFPENRTR PHKEILEFPI 550
    REVYVPHTVY RNLLYVYPQR LNFASKLASA RNITIKIQFM CGEDPSNAMP 600
    VIFGKSSGPE FLQEVYTAIT YHNKSPDFYE EVKIKLPAKL TVNHHLLFTF 650
    YHISCQQKQG ASGESLLGYS WLPILLNERL QTGSYCLPVA LEKLPPNYSI 700
    HSAEKVPLQN PPIKWAEGHK GVFNIEVQAV SSVHTQDNHL EKFFTLCHSL 750
    ESQVSFPIRV LDQKITESTL EHELKLSIIC LNSSRLEPLV LFLHLVLDKL 800
    FQLSVQPMVI AGQTANFSQF AFESVVAIAN SLHNSKDLRK DQHGRNCLLA 850
    SYVHYVFRLP ELHRDTSKSG GPTTVVPDPR YHTYGRTSAA AVSSKLMQAR 900
    VMSSSNPDLT GSHCAADEEV KNIMSSKIAD RNCSRMSYYC SGNSDAPGST 950
    AAPRPVSKKH FHEELALQMV VSTGVVRETV FKYAWFFFEL LVKSMAQYVH 1000
    NLDKRDSFRR TRFSDRFKDD ITTIVNVVTS EIAALLVKPQ KESEQAEKIN 1050
    ISLAFFLYDL LSIMDRGFVF NLIKHYCSQL SAKLNILPTL ISMRLEFLRI 1100
    LCSHEHYLNL NLLFMNTDTA PASPCPSISS QNSSSCSSFQ DQKIASMFDL 1150
    TPEYRQQHFL TGLLFTELAV ALDAEGDGIS RVQRKAVSAI HSLLCSHDLD 1200
    PRCRKPEVKV KIAALYLPLV GIILDALPQL YDFTDARSGR SRASGSYEEQ 1250
    DVANGINQNV ALAIAGNHFN LKTSGAMLSS LPYKQYNMLN ADTTRHLMIC 1300
    FLWIMKNADQ SLIRKWIADL PSMQLNRILD LLFICVSCFE YKGKQSSDKV 1350
    SNQVLQKSRD VKAKLEEALL RGEGARGEMM RRRIPGTDRF PGINENLRWR 1400
    KEQTQWRQAN EKLDKTKAEL DQEALISGNL ATEANLIILD MQENIIQASS 1450
    ALDCKDSLLG GVLRVLVNSL SCDQSTTYLT HCFATLRALI AKFGDLLFEE 1500
    EMEQCADLCQ RVLHHCSSSM DVTRSQACAT LYLLMRFSFG ATSNFARVKM 1550
    QVTMALASLV GKAPDFNEEH LRRSLRTILA YSEEDTAMQT TPFPMQVEEL 1600
    LCNLNSILYD TVKMREFQED PEMLMDLMYR IAKSYQASPD LRLTWLQNMA 1650
    EKHTKKKCFT EAAMCLVHAA ALVAEYLSML EDHSYLPVGS VSFQNISSNV 1700
    LEESAVSDDT LSPDEDGVCS GRYFTESGLV GLLEQAAELF STGGLYETVN 1750
    EVYKLVIPIL EAHRDFRKLT STHDKLQKAF DNIINKDHKR MFGTYFRVGF 1800
    YGSRFGDLDE QEFVYKEPAI TKLPEISHRL EGFYGQCFGA EFVEVIKDST 1850
    PVDKTKLDPN KAYIQITFVE PYFDEYEMKD RVTYFEKNFN LRRFMYTTPF 1900
    TLEGRPRGEL HEQHRRNTVL TTMHAFPYIK TRIRVSQKEE FVLTPIEVAI 1950
    EDMKKKTLQL AVATHQEPPD AKMLQMVLQG SVGATVNQGP LEVAQVFLAE 2000
    IPADPKLYRH HNKLRLCFKE FIMRCGEAVE KNRRLITAEQ REYQQELKKN 2050
    YNKLRDSLRP MIERKIPELY KPIFRVDSQK RDSFHRSSFR KCETQLSQGS 2100
    Length:2,100
    Mass (Da):238,978
    Last modified:August 21, 2007 - v4
    Checksum:iE867E04339CC6009
    GO
    Isoform 2 (identifier: Q8C147-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-366: Missing.
         1042-1044: ESE → VAV
         1045-2100: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:678
    Mass (Da):76,556
    Checksum:i56753BFF16C1FB5F
    GO

    Sequence cautioni

    The sequence AAH30316.2 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB23587.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC26219.2 differs from that shown. Reason: Erroneous initiation.
    The sequence BAD21430.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1608 – 16081L → M in BAC26219. (PubMed:16141072)Curated
    Sequence conflicti1616 – 16161E → K in AAH43470. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 366366Missing in isoform 2. 1 PublicationVSP_027374Add
    BLAST
    Alternative sequencei1042 – 10443ESE → VAV in isoform 2. 1 PublicationVSP_027375
    Alternative sequencei1045 – 21001056Missing in isoform 2. 1 PublicationVSP_027376Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC139711 Genomic DNA. No translation available.
    AC132319 Genomic DNA. No translation available.
    AK004816 mRNA. Translation: BAB23587.1. Different initiation.
    AK028968 mRNA. Translation: BAC26219.2. Different initiation.
    AK038285 mRNA. Translation: BAC29959.1.
    BC029018 mRNA. Translation: AAH29018.2.
    BC030316 mRNA. Translation: AAH30316.2. Different initiation.
    BC043470 mRNA. Translation: AAH43470.1.
    BC055295 mRNA. Translation: AAH55295.1.
    AK131180 mRNA. Translation: BAD21430.1. Different initiation.
    CCDSiCCDS37943.1. [Q8C147-1]
    RefSeqiNP_083061.2. NM_028785.3. [Q8C147-1]
    UniGeneiMm.102485.

    Genome annotation databases

    EnsembliENSMUST00000025831; ENSMUSP00000025831; ENSMUSG00000052085. [Q8C147-1]
    GeneIDi76088.
    KEGGimmu:76088.
    UCSCiuc008hba.1. mouse. [Q8C147-1]
    uc008hbc.1. mouse. [Q8C147-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC139711 Genomic DNA. No translation available.
    AC132319 Genomic DNA. No translation available.
    AK004816 mRNA. Translation: BAB23587.1 . Different initiation.
    AK028968 mRNA. Translation: BAC26219.2 . Different initiation.
    AK038285 mRNA. Translation: BAC29959.1 .
    BC029018 mRNA. Translation: AAH29018.2 .
    BC030316 mRNA. Translation: AAH30316.2 . Different initiation.
    BC043470 mRNA. Translation: AAH43470.1 .
    BC055295 mRNA. Translation: AAH55295.1 .
    AK131180 mRNA. Translation: BAD21430.1 . Different initiation.
    CCDSi CCDS37943.1. [Q8C147-1 ]
    RefSeqi NP_083061.2. NM_028785.3. [Q8C147-1 ]
    UniGenei Mm.102485.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VHL X-ray 2.08 A 1787-2067 [» ]
    ProteinModelPortali Q8C147.
    SMRi Q8C147. Positions 1637-2066.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 217953. 1 interaction.

    PTM databases

    PhosphoSitei Q8C147.

    Proteomic databases

    MaxQBi Q8C147.
    PaxDbi Q8C147.
    PRIDEi Q8C147.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025831 ; ENSMUSP00000025831 ; ENSMUSG00000052085 . [Q8C147-1 ]
    GeneIDi 76088.
    KEGGi mmu:76088.
    UCSCi uc008hba.1. mouse. [Q8C147-1 ]
    uc008hbc.1. mouse. [Q8C147-2 ]

    Organism-specific databases

    CTDi 81704.
    MGIi MGI:1921396. Dock8.

    Phylogenomic databases

    eggNOGi NOG242127.
    GeneTreei ENSGT00740000115071.
    HOVERGENi HBG051390.
    InParanoidi Q8C147.
    OMAi QDNHLEK.
    OrthoDBi EOG7P8P7G.
    PhylomeDBi Q8C147.
    TreeFami TF313629.

    Enzyme and pathway databases

    Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

    Miscellaneous databases

    ChiTaRSi DOCK8. mouse.
    NextBioi 344573.
    PROi Q8C147.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8C147.
    Bgeei Q8C147.
    CleanExi MM_DOCK8.
    Genevestigatori Q8C147.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR027007. DHR-1_domain.
    IPR027357. DHR-2.
    IPR026791. DOCK.
    IPR010703. DOCK_C.
    IPR021816. DOCK_C/D_N.
    [Graphical view ]
    PANTHERi PTHR23317. PTHR23317. 1 hit.
    Pfami PF06920. Ded_cyto. 1 hit.
    PF14429. DOCK-C2. 1 hit.
    PF11878. DUF3398. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS51650. DHR_1. 1 hit.
    PS51651. DHR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1581-2100 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Lung, Skin and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 967-2100 (ISOFORM 1).
      Strain: C57BL/6J, FVB/N and FVB/N-3.
      Tissue: Mammary gland.
    4. "Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
      DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 992-2100 (ISOFORM 1).
      Strain: ICR.
      Tissue: Embryonic tail.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDOCK8_MOUSE
    AccessioniPrimary (citable) accession number: Q8C147
    Secondary accession number(s): Q6KAM7
    , Q6PIS0, Q7TMQ5, Q8CAP6, Q8K105, Q9DBQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 3, 2003
    Last sequence update: August 21, 2007
    Last modified: October 1, 2014
    This is version 86 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3