Q8C147 (DOCK8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dedicator of cytokinesis protein 8

Gene names

Name:

Dock8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2100 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP By similarity.
Domain	The DHR-2 domain may mediate some GEF activity By similarity.
Sequence similarities	Belongs to the DOCK family. Contains 1 DHR-1 domain. Contains 1 DHR-2 domain.
Sequence caution	The sequence AAH30316.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB23587.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC26219.2 differs from that shown. Reason: Erroneous initiation. The sequence BAD21430.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8C147-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8C147-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-366: Missing.
     1042-1044: ESE → VAV
     1045-2100: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 2100

2100

Dedicator of cytokinesis protein 8

PRO_0000189998

Regions

Domain

561 – 730

170

DHR-1

Domain

1633 – 2067

435

DHR-2

Amino acid modifications

Modified residue

452

1

Phosphoserine By similarity

Natural variations

Alternative sequence

1 – 366

366

Missing in isoform 2.

VSP_027374

Alternative sequence

1042 – 1044

3

ESE → VAV in isoform 2.

VSP_027375

Alternative sequence

1045 – 2100

1056

Missing in isoform 2.

VSP_027376

Experimental info

Sequence conflict

1608

1

L → M in BAC26219. Ref.2

Sequence conflict

1616

1

E → K in AAH43470. Ref.3

Secondary structure

1

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2100

Helix Strand Turn

Details...

Beta strand

1794 – 1802

9

Helix

1803 – 1808

6

Beta strand

1812 – 1820

9

Helix

1823 – 1838

16

Helix

1840 – 1842

3

Beta strand

1843 – 1846

4

Helix

1854 – 1856

3

Beta strand

1861 – 1871

11

Helix

1875 – 1878

4

Beta strand

1891 – 1900

10

Beta strand

1904 – 1906

3

Helix

1910 – 1912

3

Beta strand

1914 – 1927

14

Beta strand

1929 – 1943

15

Helix

1945 – 1965

21

Helix

1971 – 1982

12

Beta strand

1985 – 1987

3

Helix

1990 – 1997

8

Helix

2005 – 2035

31

Helix

2038 – 2040

3

Helix

2041 – 2062

22

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 21, 2007. Version 4. Checksum: E867E04339CC6009	FASTA	2,100	238,978
	10 20 30 40 50 60 MATLPSAERR AFALKINRYS SSEIRKQFTL PPNLGQYHRH SISTSGFPSL QLPQLYEPVE 70 80 90 100 110 120 PVDFEGLVMT HLNSLDAELA QELGDLTDDD LHVAFTPKEC RTLQHSLPEE GVELDPHVRD 130 140 150 160 170 180 CVQTYIREWL IVNRKNQGSS EFCSFKKTGS RRDFQKTLQK QTFESETLEC SEPDTQTGPR 190 200 210 220 230 240 HPLNVLCDVS GKGPLTSCDF DLRSLQPDER LENLLQLVSA EDFEKEKEEA RKTNRPAELF 250 260 270 280 290 300 ALYPPVDEED AVEIRPVPEC PKEHLGNRIL VKVLTLKFEI EIEPLFASIA LYDVKERKKI 310 320 330 340 350 360 SENFHCDLNS DQFKGFLRAH TPSIDPSSQA RSAVFSVTYP SSDIYLVVKI EKVLQQGEIA 370 380 390 400 410 420 DCAEPYMIIK ESDGGKSKEK VEKLKLQAES FCQRLGKYRM PFAWAPISLA SFFNISTLER 430 440 450 460 470 480 ESTDVEPGVG RNSVGEKRSL SQSRRPSERT LSLEENGVGS NFKATTLATN IFFKQEGDRL 490 500 510 520 530 540 SDEDLFKFLA DYKRSSSLQR RVKSIPGSLR LEISPAPDVM NCCLTPEMLP VKPFPENRTR 550 560 570 580 590 600 PHKEILEFPI REVYVPHTVY RNLLYVYPQR LNFASKLASA RNITIKIQFM CGEDPSNAMP 610 620 630 640 650 660 VIFGKSSGPE FLQEVYTAIT YHNKSPDFYE EVKIKLPAKL TVNHHLLFTF YHISCQQKQG 670 680 690 700 710 720 ASGESLLGYS WLPILLNERL QTGSYCLPVA LEKLPPNYSI HSAEKVPLQN PPIKWAEGHK 730 740 750 760 770 780 GVFNIEVQAV SSVHTQDNHL EKFFTLCHSL ESQVSFPIRV LDQKITESTL EHELKLSIIC 790 800 810 820 830 840 LNSSRLEPLV LFLHLVLDKL FQLSVQPMVI AGQTANFSQF AFESVVAIAN SLHNSKDLRK 850 860 870 880 890 900 DQHGRNCLLA SYVHYVFRLP ELHRDTSKSG GPTTVVPDPR YHTYGRTSAA AVSSKLMQAR 910 920 930 940 950 960 VMSSSNPDLT GSHCAADEEV KNIMSSKIAD RNCSRMSYYC SGNSDAPGST AAPRPVSKKH 970 980 990 1000 1010 1020 FHEELALQMV VSTGVVRETV FKYAWFFFEL LVKSMAQYVH NLDKRDSFRR TRFSDRFKDD 1030 1040 1050 1060 1070 1080 ITTIVNVVTS EIAALLVKPQ KESEQAEKIN ISLAFFLYDL LSIMDRGFVF NLIKHYCSQL 1090 1100 1110 1120 1130 1140 SAKLNILPTL ISMRLEFLRI LCSHEHYLNL NLLFMNTDTA PASPCPSISS QNSSSCSSFQ 1150 1160 1170 1180 1190 1200 DQKIASMFDL TPEYRQQHFL TGLLFTELAV ALDAEGDGIS RVQRKAVSAI HSLLCSHDLD 1210 1220 1230 1240 1250 1260 PRCRKPEVKV KIAALYLPLV GIILDALPQL YDFTDARSGR SRASGSYEEQ DVANGINQNV 1270 1280 1290 1300 1310 1320 ALAIAGNHFN LKTSGAMLSS LPYKQYNMLN ADTTRHLMIC FLWIMKNADQ SLIRKWIADL 1330 1340 1350 1360 1370 1380 PSMQLNRILD LLFICVSCFE YKGKQSSDKV SNQVLQKSRD VKAKLEEALL RGEGARGEMM 1390 1400 1410 1420 1430 1440 RRRIPGTDRF PGINENLRWR KEQTQWRQAN EKLDKTKAEL DQEALISGNL ATEANLIILD 1450 1460 1470 1480 1490 1500 MQENIIQASS ALDCKDSLLG GVLRVLVNSL SCDQSTTYLT HCFATLRALI AKFGDLLFEE 1510 1520 1530 1540 1550 1560 EMEQCADLCQ RVLHHCSSSM DVTRSQACAT LYLLMRFSFG ATSNFARVKM QVTMALASLV 1570 1580 1590 1600 1610 1620 GKAPDFNEEH LRRSLRTILA YSEEDTAMQT TPFPMQVEEL LCNLNSILYD TVKMREFQED 1630 1640 1650 1660 1670 1680 PEMLMDLMYR IAKSYQASPD LRLTWLQNMA EKHTKKKCFT EAAMCLVHAA ALVAEYLSML 1690 1700 1710 1720 1730 1740 EDHSYLPVGS VSFQNISSNV LEESAVSDDT LSPDEDGVCS GRYFTESGLV GLLEQAAELF 1750 1760 1770 1780 1790 1800 STGGLYETVN EVYKLVIPIL EAHRDFRKLT STHDKLQKAF DNIINKDHKR MFGTYFRVGF 1810 1820 1830 1840 1850 1860 YGSRFGDLDE QEFVYKEPAI TKLPEISHRL EGFYGQCFGA EFVEVIKDST PVDKTKLDPN 1870 1880 1890 1900 1910 1920 KAYIQITFVE PYFDEYEMKD RVTYFEKNFN LRRFMYTTPF TLEGRPRGEL HEQHRRNTVL 1930 1940 1950 1960 1970 1980 TTMHAFPYIK TRIRVSQKEE FVLTPIEVAI EDMKKKTLQL AVATHQEPPD AKMLQMVLQG 1990 2000 2010 2020 2030 2040 SVGATVNQGP LEVAQVFLAE IPADPKLYRH HNKLRLCFKE FIMRCGEAVE KNRRLITAEQ 2050 2060 2070 2080 2090 2100 REYQQELKKN YNKLRDSLRP MIERKIPELY KPIFRVDSQK RDSFHRSSFR KCETQLSQGS
	Isoform 2 [UniParc]. Checksum: 56753BFF16C1FB5F	FASTA	678	76,556
	10 20 30 40 50 60 MIIKESDGGK SKEKVEKLKL QAESFCQRLG KYRMPFAWAP ISLASFFNIS TLERESTDVE 70 80 90 100 110 120 PGVGRNSVGE KRSLSQSRRP SERTLSLEEN GVGSNFKATT LATNIFFKQE GDRLSDEDLF 130 140 150 160 170 180 KFLADYKRSS SLQRRVKSIP GSLRLEISPA PDVMNCCLTP EMLPVKPFPE NRTRPHKEIL 190 200 210 220 230 240 EFPIREVYVP HTVYRNLLYV YPQRLNFASK LASARNITIK IQFMCGEDPS NAMPVIFGKS 250 260 270 280 290 300 SGPEFLQEVY TAITYHNKSP DFYEEVKIKL PAKLTVNHHL LFTFYHISCQ QKQGASGESL 310 320 330 340 350 360 LGYSWLPILL NERLQTGSYC LPVALEKLPP NYSIHSAEKV PLQNPPIKWA EGHKGVFNIE 370 380 390 400 410 420 VQAVSSVHTQ DNHLEKFFTL CHSLESQVSF PIRVLDQKIT ESTLEHELKL SIICLNSSRL 430 440 450 460 470 480 EPLVLFLHLV LDKLFQLSVQ PMVIAGQTAN FSQFAFESVV AIANSLHNSK DLRKDQHGRN 490 500 510 520 530 540 CLLASYVHYV FRLPELHRDT SKSGGPTTVV PDPRYHTYGR TSAAAVSSKL MQARVMSSSN 550 560 570 580 590 600 PDLTGSHCAA DEEVKNIMSS KIADRNCSRM SYYCSGNSDA PGSTAAPRPV SKKHFHEELA 610 620 630 640 650 660 LQMVVSTGVV RETVFKYAWF FFELLVKSMA QYVHNLDKRD SFRRTRFSDR FKDDITTIVN 670 VVTSEIAALL VKPQKVAV

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1581-2100 (ISOFORM 1). Strain: C57BL/6J. Tissue: Lung, Skin and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 967-2100 (ISOFORM 1). Strain: C57BL/6J, FVB/N and FVB/N-3. Tissue: Mammary gland.
[4]	"Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H. DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 992-2100 (ISOFORM 1). Strain: ICR. Tissue: Embryonic tail.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AC139711 Genomic DNA. No translation available.
AC132319 Genomic DNA. No translation available.
AK004816 mRNA. Translation: BAB23587.1. Different initiation.
AK028968 mRNA. Translation: BAC26219.2. Different initiation.
AK038285 mRNA. Translation: BAC29959.1.
BC029018 mRNA. Translation: AAH29018.2.
BC030316 mRNA. Translation: AAH30316.2. Different initiation.
BC043470 mRNA. Translation: AAH43470.1.
BC055295 mRNA. Translation: AAH55295.1.
AK131180 mRNA. Translation: BAD21430.1. Different initiation.

IPI

IPI00228136.
IPI00475383.

RefSeq

NP_083061.2. NM_028785.3.

UniGene

Mm.102485.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3VHL	X-ray	2.08	A	1787-2067	[»]

ProteinModelPortal

Q8C147.

SMR

Q8C147. Positions 1637-2066.

ModBase

Search...

PTM databases

PhosphoSite

Q8C147.

Proteomic databases

PaxDb

Q8C147.

PRIDE

Q8C147.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000025831; ENSMUSP00000025831; ENSMUSG00000052085.

GeneID

76088.

KEGG

mmu:76088.

UCSC

uc008hba.1. mouse.
uc008hbc.1. mouse.

Organism-specific databases

CTD

81704.

MGI

MGI:1921396. Dock8.

Phylogenomic databases

eggNOG

NOG242127.

GeneTree

ENSGT00560000076710.

HOVERGEN

HBG051390.

InParanoid

Q8C147.

OMA

SVHTQDN.

OrthoDB

EOG42Z4P8.

Gene expression databases

ArrayExpress

Q8C147.

Bgee

Q8C147.

CleanEx

MM_DOCK8.

Genevestigator

Q8C147.

GermOnline

ENSMUSG00000052085. Mus musculus.

Family and domain databases

InterPro

IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
IPR026798. DOCK_C_fam.
[Graphical view]

PANTHER

PTHR23317. PTHR23317. 1 hit.
PTHR23317:SF27. PTHR23317:SF27. 1 hit.

Pfam

PF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view]

PROSITE

PS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

DOCK8. mouse.

NextBio

344573.

SOURCE

Search...

Entry information

Entry name

DOCK8_MOUSE

Accession

Primary (citable) accession number: Q8C147
Secondary accession number(s): Q6KAM7 , Q6PIS0, Q7TMQ5, Q8CAP6, Q8K105, Q9DBQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	August 21, 2007
Last modified:	May 1, 2013
This is version 73 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program