Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hydroxycarboxylic acid receptor 1

Gene

Hcar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxycarboxylic acid receptor 1
Alternative name(s):
G-protein coupled receptor 81
Gene namesi
Name:Hcar1
Synonyms:Gpr81
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2441671. Hcar1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121ExtracellularSequence analysisAdd
BLAST
Transmembranei22 – 4221Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini43 – 497CytoplasmicSequence analysis
Transmembranei50 – 7021Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini71 – 9020ExtracellularSequence analysisAdd
BLAST
Transmembranei91 – 11121Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini112 – 13120CytoplasmicSequence analysisAdd
BLAST
Transmembranei132 – 15221Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini153 – 18230ExtracellularSequence analysisAdd
BLAST
Transmembranei183 – 20321Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini204 – 22017CytoplasmicSequence analysisAdd
BLAST
Transmembranei221 – 24121Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini242 – 25918ExtracellularSequence analysisAdd
BLAST
Transmembranei260 – 28021Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini281 – 34363CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2146354.
GuidetoPHARMACOLOGYi311.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Hydroxycarboxylic acid receptor 1PRO_0000069587Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi3 – 31N-linked (GlcNAc...)Sequence analysis
Disulfide bondi88 ↔ 165PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8C131.
PRIDEiQ8C131.

PTM databases

PhosphoSiteiQ8C131.

Expressioni

Tissue specificityi

Highly expressed in subcutaneous fat and omental fat and detectable in lower levels in brain and many other tissues. High levels detected in epididymal and subcutaneous fat with slightly lower in omental fat, low levels are detected in the brain, skeletal muscle, kidney, liver and the pancreas (at protein level).2 Publications

Gene expression databases

BgeeiQ8C131.
CleanExiMM_GPR81.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000129280.

Chemistry

BindingDBiQ8C131.

Structurei

3D structure databases

ProteinModelPortaliQ8C131.
SMRiQ8C131. Positions 18-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKI5. Eukaryota.
ENOG4110VUM. LUCA.
HOVERGENiHBG051680.
InParanoidiQ8C131.
PhylomeDBiQ8C131.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR028016. HCAR1_rcpt.
[Graphical view]
PANTHERiPTHR24231:SF29. PTHR24231:SF29. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNGSCCLIE GEPISQVMPP LLILVFVLGA LGNGIALCGF CFHMKTWKSS
60 70 80 90 100
TIYLFNLAVA DFLLMICLPL RTDYYLRRRH WIFGDIACRL VLFKLAMNRA
110 120 130 140 150
GSIVFLTVVA VDRYFKVVHP HHMVNAISNR TAAATACVLW TLVILGTVYL
160 170 180 190 200
LMESHLCVQG TLSSCESFIM ESANGWHDVM FQLEFFLPLT IILFCSVNVV
210 220 230 240 250
WSLRRRQQLT RQARMRRATR FIMVVASVFI TCYLPSVLAR LYFLWTVPTS
260 270 280 290 300
ACDPSVHTAL HVTLSFTYLN SMLDPLVYYF SSPSLPKFYT KLTICSLKPK
310 320 330 340
RPGRTKTRRS EEMPISNLCS KSSIDGANRS QRPSDGQWDL QVC
Length:343
Mass (Da):38,927
Last modified:March 1, 2003 - v1
Checksum:i917FA9499B2E03FD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti290 – 2901T → A in ACJ03846 (PubMed:19047060).Curated
Sequence conflicti290 – 2901T → A in AAI41306 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU809460 mRNA. Translation: ACJ03846.1.
AK029064 mRNA. Translation: BAC26273.1.
BC141305 mRNA. Translation: AAI41306.1.
UniGeneiMm.33362.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU809460 mRNA. Translation: ACJ03846.1.
AK029064 mRNA. Translation: BAC26273.1.
BC141305 mRNA. Translation: AAI41306.1.
UniGeneiMm.33362.

3D structure databases

ProteinModelPortaliQ8C131.
SMRiQ8C131. Positions 18-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000129280.

Chemistry

BindingDBiQ8C131.
ChEMBLiCHEMBL2146354.
GuidetoPHARMACOLOGYi311.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ8C131.

Proteomic databases

PaxDbiQ8C131.
PRIDEiQ8C131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:2441671. Hcar1.

Phylogenomic databases

eggNOGiENOG410IKI5. Eukaryota.
ENOG4110VUM. LUCA.
HOVERGENiHBG051680.
InParanoidiQ8C131.
PhylomeDBiQ8C131.

Miscellaneous databases

PROiQ8C131.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C131.
CleanExiMM_GPR81.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR028016. HCAR1_rcpt.
[Graphical view]
PANTHERiPTHR24231:SF29. PTHR24231:SF29. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Lactate inhibits lipolysis in fat cells through activation of an orphan G-protein-coupled receptor, GPR81."
    Liu C., Wu J., Zhu J., Kuei C., Yu J., Shelton J., Sutton S.W., Li X., Yun S.J., Mirzadegan T., Mazur C., Kamme F., Lovenberg T.W.
    J. Biol. Chem. 284:2811-2822(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, FUNCTION.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Elucidation of signaling and functional activities of an orphan GPCR, GPR81."
    Ge H., Weiszmann J., Reagan J.D., Gupte J., Baribault H., Gyuris T., Chen J.-L., Tian H., Li Y.
    J. Lipid Res. 49:797-803(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue.

Entry informationi

Entry nameiHCAR1_MOUSE
AccessioniPrimary (citable) accession number: Q8C131
Secondary accession number(s): B9EJ36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: March 1, 2003
Last modified: February 17, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.