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Protein

Leucyl-cystinyl aminopeptidase

Gene

Lnpep

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Release of an N-terminal amino acid, cleave before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Cys-|-Xaa-, in which the half-cystine residue is involved in a disulfide loop, notably in oxytocin or vasopressin. Hydrolysis rates on a range of aminoacyl arylamides exceed that for the cystinyl derivative, however.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei295SubstrateBy similarity1
Metal bindingi464Zinc; catalyticPROSITE-ProRule annotation1
Active sitei465Proton acceptorPROSITE-ProRule annotation1
Metal bindingi468Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi487Zinc; catalyticPROSITE-ProRule annotation1
Sitei549Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: MGI
  • metalloaminopeptidase activity Source: GO_Central
  • peptide binding Source: GO_Central
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • cell-cell signaling Source: GO_Central
  • peptide catabolic process Source: GO_Central
  • protein catabolic process Source: MGI
  • regulation of blood pressure Source: GO_Central
  • SMAD protein signal transduction Source: MGI

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-1236977 Endosomal/Vacuolar pathway
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiM01.011

Names & Taxonomyi

Protein namesi
Recommended name:
Leucyl-cystinyl aminopeptidase (EC:3.4.11.3)
Short name:
Cystinyl aminopeptidase
Alternative name(s):
Oxytocinase
Short name:
OTase
Gene namesi
Name:Lnpep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2387123 Lnpep

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 109CytoplasmicSequence analysisAdd BLAST109
Transmembranei110 – 131Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST22
Topological domaini132 – 1025ExtracellularSequence analysisAdd BLAST894

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002781981 – 1025Leucyl-cystinyl aminopeptidaseAdd BLAST1025

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei70PhosphotyrosineCombined sources1
Modified residuei80PhosphoserineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi184N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi215N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi256N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi266N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi368N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi374N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi447N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi525N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi578N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi664N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi682N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi695N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi758N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi834N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi850N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi989N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8C129
MaxQBiQ8C129
PaxDbiQ8C129
PeptideAtlasiQ8C129
PRIDEiQ8C129

PTM databases

iPTMnetiQ8C129
PhosphoSitePlusiQ8C129
SwissPalmiQ8C129

Expressioni

Gene expression databases

BgeeiENSMUSG00000023845
CleanExiMM_LNPEP
GenevisibleiQ8C129 MM

Interactioni

Subunit structurei

Homodimer. Binds tankyrases 1 and 2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi232146, 2 interactors
IntActiQ8C129, 1 interactor
MINTiQ8C129
STRINGi10090.ENSMUSP00000036998

Structurei

3D structure databases

ProteinModelPortaliQ8C129
SMRiQ8C129
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 101Tankyrase bindingBy similarity6
Regioni428 – 432Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi53 – 54Dileucine internalization motifSequence analysis2
Motifi76 – 77Dileucine internalization motifSequence analysis2

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1046 Eukaryota
COG0308 LUCA
GeneTreeiENSGT00760000119082
HOGENOMiHOG000106482
HOVERGENiHBG108296
InParanoidiQ8C129
KOiK01257
OMAiMMKTWTL
OrthoDBiEOG091G01GH
PhylomeDBiQ8C129
TreeFamiTF300395

Family and domain databases

CDDicd09601 M1_APN_2, 1 hit
InterProiView protein in InterPro
IPR034017 Cystinyl_aminopeptidase
IPR024571 ERAP1-like_C_dom
IPR034016 M1_APN-typ
IPR001930 Peptidase_M1
IPR014782 Peptidase_M1_N
PANTHERiPTHR11533 PTHR11533, 1 hit
PTHR11533:SF42 PTHR11533:SF42, 1 hit
PfamiView protein in Pfam
PF11838 ERAP1_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8C129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESFTNDRLQ LPRNMIENSM FEEEPDVVDL AKEPCLHPLE PDEVEYEPRG
60 70 80 90 100
SRLLVRGLGE HEMDEDEEDY ESSAKLLGMS FMNRSSGLRN SAAGYRQSPD
110 120 130 140 150
GTCSLPSART LVICVFVIVV AVSVIMVIYL LPRCTFTKEG CHKTNQSAEL
160 170 180 190 200
IQPVATNGKV FPWAQIRLPT AIIPLCYELS LHPNLTSMTF RGSVTISLQA
210 220 230 240 250
LQDTRDIILH STGHNISRVT FMSAVSSQEK QVEILEYPYH EQIAVVAPEP
260 270 280 290 300
LLTGHNYTLK IEYSANISNS YYGFYGITYT DKSNEKKYFA ATQFEPLAAR
310 320 330 340 350
SAFPCFDEPA FKATFIIKIT RNEHHTALSN MPKKSSVPAE EGLIQDEFSE
360 370 380 390 400
SVKMSTYLVA FIVGEMRNLS QDVNGTLVSV YAVPEKIGQV HHALDTTIKL
410 420 430 440 450
LEFYQTYFEI QYPLKKLDLV AIPDFEAGAM ENWGLLTFRE ETLLYDNATS
460 470 480 490 500
SVADRKLVTK IIAHELAHQW FGNLVTMQWW NDLWLNEGFA TFMEYFSVEK
510 520 530 540 550
IFKELNSYED FLDARFKTMR KDSLNSSHPI SSSVQSSEQI EEMFDSLSYF
560 570 580 590 600
KGASLLLMLK SYLSEDVFRH AVILYLHNHS YAAIQSDDLW DSFNEVTDKT
610 620 630 640 650
LDVKKMMKTW TLQKGFPLVT VQRKGTELLL QQERFFLRMQ PESQPSDTSH
660 670 680 690 700
LWHIPISYVT DGRNYSEYRS VSLLDKKSDV INLTEQVQWV KVNSNMTGYY
710 720 730 740 750
IVHYAHDDWT ALINQLKRNP YVLSDKDRAN LINNIFELAG LGKVPLRMAF
760 770 780 790 800
DLIDYLKNET HTAPITEALF QTNLIYNLLE KLGHMDLSSR LVARVHKLLQ
810 820 830 840 850
NQIQQQTWTD EGTPSMRELR SALLEFACAH SLENCTTMAT NLFDSWMASN
860 870 880 890 900
GTQSLPTDVM VTVFKVGART EKGWLFLFSM YSSMGSEAEK NKILEALASS
910 920 930 940 950
EDVHKLYWLM KSSLDGDIIR TQKLSLIIRT VGRHFPGHLL AWDFVKENWN
960 970 980 990 1000
KLVHKFHLGS YTIQSIVAGS THLFSTKTHL SEVQAFFENQ SEATLKLRCV
1010 1020
QEALEVIQLN IQWMVRNLKT LSQWL
Length:1,025
Mass (Da):117,304
Last modified:March 1, 2003 - v1
Checksum:iAD6F98196EBA683B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029094 mRNA Translation: BAC26293.1
BC120925 mRNA Translation: AAI20926.1
BC120926 mRNA Translation: AAI20927.1
CCDSiCCDS37457.1
RefSeqiNP_766415.1, NM_172827.3
UniGeneiMm.328807
Mm.393452
Mm.444774

Genome annotation databases

EnsembliENSMUST00000041047; ENSMUSP00000036998; ENSMUSG00000023845
GeneIDi240028
KEGGimmu:240028
UCSCiuc008apk.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLCAP_MOUSE
AccessioniPrimary (citable) accession number: Q8C129
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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