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Protein

Sodium/hydrogen exchanger 9B1

Gene

Slc9b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sperm-specific Na+/H+ exchanger involved in intracellular pH regulation of spermatozoa (PubMed:20036903). Involved in sperm motility and fertility (PubMed:20036903, PubMed:27010853, PubMed:19409551).3 Publications

GO - Molecular functioni

GO - Biological processi

  • flagellated sperm motility Source: UniProtKB
  • regulation of intracellular pH Source: UniProtKB
  • single fertilization Source: UniProtKB-KW
  • sodium ion transport Source: UniProtKB-KW

Keywordsi

Biological processAntiport, Fertilization, Ion transport, Sodium transport, Transport
LigandSodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 9B1
Alternative name(s):
Na(+)/H(+) exchanger-like domain-containing protein 1
Short name:
NHE domain-containing protein 1
Sodium/hydrogen exchanger-like domain-containing protein 1
Solute carrier family 9 subfamily B member 1
Testis specific sodium-hydrogen exchanger2 Publications
Short name:
MtsNHE2 Publications
Gene namesi
Name:Slc9b1Imported
Synonyms:Nha11 Publication, Nhedc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1921696. Slc9b1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Transmembranei206 – 223HelicalSequence analysisAdd BLAST18
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Transmembranei311 – 331HelicalSequence analysisAdd BLAST21
Transmembranei341 – 361HelicalSequence analysisAdd BLAST21
Transmembranei371 – 391HelicalSequence analysisAdd BLAST21
Transmembranei419 – 439HelicalSequence analysisAdd BLAST21
Transmembranei449 – 469HelicalSequence analysisAdd BLAST21
Transmembranei482 – 502HelicalSequence analysisAdd BLAST21
Transmembranei523 – 543HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are normal but males shown reduced fertility caused by diminished sperm motility (PubMed:27010853). Addition of cAMP analogs almost completely rescue the motility and infertility phenotypes in vitro (PubMed:27010853). Double knockout of SLC9B1 and SLC9B2 results in male infertility with a severe sperm mobility reduction, indicating that these two gene are functionally redundant (PubMed:27010853).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003140091 – 565Sodium/hydrogen exchanger 9B1Add BLAST565

Proteomic databases

PaxDbiQ8C0X2.
PRIDEiQ8C0X2.

PTM databases

iPTMnetiQ8C0X2.
PhosphoSitePlusiQ8C0X2.

Expressioni

Tissue specificityi

Testis-specific (PubMed:20036903). Expressed in the spermatids and spermatozoa (at protein level) (PubMed:20036903). Specifically present in the principal piece of sperm tail (at protein level) (PubMed:20036903, PubMed:27010853, PubMed:19409551).3 Publications

Gene expression databases

CleanExiMM_NHEDC1.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077644.

Structurei

3D structure databases

ProteinModelPortaliQ8C0X2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi32 – 98Glu-richAdd BLAST67

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3826. Eukaryota.
COG0025. LUCA.
HOGENOMiHOG000060325.
HOVERGENiHBG108184.
InParanoidiQ8C0X2.
PhylomeDBiQ8C0X2.

Family and domain databases

InterProiView protein in InterPro
IPR006153. Cation/H_exchanger.
IPR030187. NHA.
PANTHERiPTHR31102:SF9. PTHR31102:SF9. 1 hit.
PfamiView protein in Pfam
PF00999. Na_H_Exchanger. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C0X2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEHDVESNK KDDGFQSSVT VEMSKDPDSF HEETVEPKPE LKEPEPKEPE
60 70 80 90 100
PKEPERKEPE RKEPERKEPE RKEPERKVPG RRETQTKETQ TTEIERKETK
110 120 130 140 150
KKRGTNSYCP PQGTINKTIT DGAALIALWT LLWALIGQEV LPGGNLFGLV
160 170 180 190 200
VIFYSAFLGG KILEFIKIPV VPPLPPLIGM LLAGFTIRNV PIIYEFVHIP
210 220 230 240 250
TTWSSALRNT ALTIILVRAG LGLDPQALKH LKGVCLRLSF GPCFLEACSA
260 270 280 290 300
ALFSHFIMNF PWQWGFLLGF VLGAVSPAVV VPNMLMLQEN GYGVEKGIPT
310 320 330 340 350
LLVAASSMDD IVAITGFNTF LSIVFSSGSV ISNILSSLRD VLIGVLVGIV
360 370 380 390 400
MGVFVQYFPS GDQERLTQRR AFLVLSMCIS AVLGCQHIGL HGSGGLVTLV
410 420 430 440 450
LSFMAAKRWA EEKVGIQKIV ANTWNVFQPL LFGLVGTEVS VESLESKTIG
460 470 480 490 500
MCLATLGLAL SVRILSTFVL MSFANFRFKE KVFIALSWIP KATVQAVLGP
510 520 530 540 550
LALETARVMA PHLEGYAKAV MTVAFLAILI TAPNGALLIG ILGPKILEQS
560
EVTFPLKVEL SNFHH
Length:565
Mass (Da):61,958
Last modified:March 1, 2003 - v1
Checksum:i7ECBC2E03DC90655
GO
Isoform 2 (identifier: Q8C0X2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     364-378: ERLTQRRAFLVLSMC → AASTLAYMDLEDWSH
     379-565: Missing.

Show »
Length:378
Mass (Da):41,780
Checksum:iD07602DC1F1AAF6A
GO
Isoform 3 (identifier: Q8C0X2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-565: Missing.

Show »
Length:269
Mass (Da):30,312
Checksum:iACC7AC536D5B8764
GO
Isoform 4 (identifier: Q8C0X2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     123-145: AALIALWTLLWALIGQEVLPGGN → MSNKKCTWGENEKYKMQMIRLTV
     146-565: Missing.

Show »
Length:145
Mass (Da):17,045
Checksum:iFDCCBA61E2B6ACE2
GO
Isoform 5 (identifier: Q8C0X2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-114: KVPGRRETQT...TNSYCPPQGT → SCTHCTMDST...SYFLQCFPWG
     115-565: Missing.

Show »
Length:114
Mass (Da):13,561
Checksum:i7DDDDD73DD7FC0E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167K → R in BAB30495 (PubMed:16141072).Curated1
Sequence conflicti167K → R in AAH25002 (PubMed:15489334).Curated1
Sequence conflicti263Q → G in BAC25498 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03018177 – 114KVPGR…PPQGT → SCTHCTMDSTLGPYRSRSSP WWKFVWTCSYFLQCFPWG in isoform 5. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_030182115 – 565Missing in isoform 5. 1 PublicationAdd BLAST451
Alternative sequenceiVSP_030183123 – 145AALIA…LPGGN → MSNKKCTWGENEKYKMQMIR LTV in isoform 4. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_030184146 – 565Missing in isoform 4. 1 PublicationAdd BLAST420
Alternative sequenceiVSP_030185270 – 565Missing in isoform 3. 1 PublicationAdd BLAST296
Alternative sequenceiVSP_030186364 – 378ERLTQ…VLSMC → AASTLAYMDLEDWSH in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_030187379 – 565Missing in isoform 2. 1 PublicationAdd BLAST187

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU846100 mRNA. Translation: ACF60498.1.
AK007064 mRNA. Translation: BAB24849.1.
AK015318 mRNA. Translation: BAB29794.1.
AK016883 mRNA. Translation: BAC25498.1.
AK016917 mRNA. Translation: BAB30495.1.
AK029525 mRNA. Translation: BAC26494.1.
BC025002 mRNA. Translation: AAH25002.1.
CCDSiCCDS17855.2. [Q8C0X2-1]
RefSeqiNP_083222.2. NM_028946.3.
XP_006502267.1. XM_006502204.1.
UniGeneiMm.85000.

Genome annotation databases

GeneIDi74446.
KEGGimmu:74446.
UCSCiuc008rlf.1. mouse. [Q8C0X2-4]
uc008rlj.3. mouse. [Q8C0X2-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSL9B1_MOUSE
AccessioniPrimary (citable) accession number: Q8C0X2
Secondary accession number(s): B5AFL0
, Q8C1I8, Q8R3N0, Q9CPR9, Q9D400
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: August 30, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families