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Protein

Adenylate cyclase type 10

Gene

Adcy10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of the signaling molecule cAMP. May function as sensor that mediates responses to changes in cellular bicarbonate and CO2 levels (By similarity). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (PubMed:14976244, PubMed:16054031). Involved in ciliary beat regulation (By similarity).By similarity2 Publications

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).By similarity

Enzyme regulationi

Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate. Calcium mildly increases the enzyme activity, also in the presence of magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Magnesium 1PROSITE-ProRule annotation1
Metal bindingi47Magnesium 2PROSITE-ProRule annotation1
Metal bindingi48Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei95BicarbonateBy similarity1
Metal bindingi99Magnesium 1PROSITE-ProRule annotation1
Metal bindingi99Magnesium 2PROSITE-ProRule annotation1
Binding sitei99ATPBy similarity1
Binding sitei144ATPBy similarity1
Binding sitei167Bicarbonate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei176BicarbonateBy similarity1
Binding sitei337Bicarbonate; via amide nitrogenBy similarity1
Binding sitei406ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 52ATPBy similarity6
Nucleotide bindingi412 – 416ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cAMP biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.1. 3474.
ReactomeiR-MMU-5610787. Hedgehog 'off' state.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 10 (EC:4.6.1.1)
Alternative name(s):
Germ cell soluble adenylyl cyclase
Short name:
sAC
Testicular soluble adenylyl cyclase
Gene namesi
Name:Adcy10
Synonyms:Sac, Sacy
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2660854. Adcy10.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: Ensembl
  • axon Source: Ensembl
  • basal part of cell Source: Ensembl
  • cytoplasm Source: MGI
  • cytosol Source: Ensembl
  • dendrite Source: Ensembl
  • growth cone Source: Ensembl
  • membrane Source: MGI
  • microtubule cytoskeleton Source: Ensembl
  • mitochondrion Source: UniProtKB-SubCell
  • motile cilium Source: Ensembl
  • neuronal cell body Source: Ensembl
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are infertile because of a severe sperm-motility defect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003171021 – 1614Adenylate cyclase type 10Add BLAST1614

Proteomic databases

PaxDbiQ8C0T9.
PRIDEiQ8C0T9.

PTM databases

iPTMnetiQ8C0T9.
PhosphoSitePlusiQ8C0T9.

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000026567.
CleanExiMM_ADCY10.
ExpressionAtlasiQ8C0T9. baseline and differential.
GenevisibleiQ8C0T9. MM.

Interactioni

Protein-protein interaction databases

DIPiDIP-60950N.
STRINGi10090.ENSMUSP00000027852.

Structurei

3D structure databases

ProteinModelPortaliQ8C0T9.
SMRiQ8C0T9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 179Guanylate cyclase 1PROSITE-ProRule annotationAdd BLAST138
Domaini293 – 418Guanylate cyclase 2PROSITE-ProRule annotationAdd BLAST126

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi909 – 912Poly-Glu4

Domaini

The N-terminal guanylate cyclase domains are required for enzyme activity. Fragments containing the first 470 amino acid residues are fully active.By similarity

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 2 guanylate cyclase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IEPA. Eukaryota.
ENOG410ZFMC. LUCA.
GeneTreeiENSGT00390000001322.
HOGENOMiHOG000060149.
HOVERGENiHBG103835.
InParanoidiQ8C0T9.
KOiK11265.
OMAiPLESCQC.
OrthoDBiEOG091G00BV.
TreeFamiTF329284.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR029787. Nucleotide_cyclase.
IPR027417. P-loop_NTPase.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55073. SSF55073. 2 hits.
PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C0T9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG
60 70 80 90 100
FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA
110 120 130 140 150
LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEAEEGLD IRVKIGLAAG
160 170 180 190 200
HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS
210 220 230 240 250
MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF TKCMGFMDYY PSGDHKNFLR
260 270 280 290 300
LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM
310 320 330 340 350
FKEQDKVEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF
360 370 380 390 400
PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV
410 420 430 440 450
RHEYTVIGQK VNIAARMMMY YPGIVSCDSV TYDGSNLPAY FFKELPKKVM
460 470 480 490 500
KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM
510 520 530 540 550
KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS
560 570 580 590 600
FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD EKFHCLLNDI
610 620 630 640 650
FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LAQTVREERI IFIIDEAQFV
660 670 680 690 700
DGTSWAFIEK LIRSMPIFIV MSLAPFSEVP CAAANAIMKN RNTTYITLGT
710 720 730 740 750
MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRV
760 770 780 790 800
LLFQQAETEQ KTNVTWNNMF KHSVRPTDDM QLFTSISEGQ KEVCYLVSGV
810 820 830 840 850
RLNNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP
860 870 880 890 900
CWNMKMMIKA LATLVESNVF NCFRSSKDLQ LALKQNVPTF EVHYRSLALK
910 920 930 940 950
LKEGLTYGEE EELREMEGEV VECRILRFCR PIMQKTAYEL WLKDQKKVLH
960 970 980 990 1000
LKCARFLEES AHRCNHCRNV DFIPYHHFIV DIRLNTLDMD TVKRMVTSQG
1010 1020 1030 1040 1050
FKIDEEEAIF SKSELPRKYK FPENLSITEI REKILHFFDN VILKMKSSPN
1060 1070 1080 1090 1100
DIIPLESCQC KELLQIVILP LAQHFVALEE NNKALYYFLE LASAYLILGD
1110 1120 1130 1140 1150
NYNAYMYLGE GERLLKSLTN EDSWSQTFEY ATFYSLKAEV CFNMGQMVLA
1160 1170 1180 1190 1200
KKMLRKALKL LNRMFPCNLL TLTFQMHVEK NRLSHFMNQH TQEGSVPGKK
1210 1220 1230 1240 1250
LAQLYLQASC FSLLWRIYSL NFFFHYKYYG HLAAMMEMNT SLETQNDFQI
1260 1270 1280 1290 1300
IKAYLDFSLY HHLAGYQGVW FKYEILVMEQ LLNLPLKGEA IEIMAYTADT
1310 1320 1330 1340 1350
LGHIKFLMGH LDLAIELGSR AHRMWSLLRN PNKYQMVLCR LSKPLFLKSR
1360 1370 1380 1390 1400
YKHLVQVLGW LWDLSVTEED IFSKAFFYFV CLDIMLYSGF IYRTFEECLE
1410 1420 1430 1440 1450
FIHHNEDNRI LKFQSGLLLG LYSCIAVWYA RLQEWDNFNK FSDRAKHLVT
1460 1470 1480 1490 1500
RRTPTVLYYE GISRYMEGQV LHLQKQIEEQ AENAQDSGVE ILKALETLVA
1510 1520 1530 1540 1550
QNTTGPVFYP RLYHLMAYVC ILMGDGHSCD FFLNTALELS ETHGNLLEKC
1560 1570 1580 1590 1600
WLSMSKEWWY SASELTGDQW LQTVLSLPSW DKIVSGKGGQ RKRSWSWFCP
1610
PNFSMVSWSQ PQCA
Length:1,614
Mass (Da):186,412
Last modified:July 27, 2011 - v2
Checksum:iAE56433825277126
GO
Isoform 2 (identifier: Q8C0T9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-471: MF → TC
     472-1614: Missing.

Note: No experimental confirmation available.
Show »
Length:471
Mass (Da):52,952
Checksum:iBE78B5B846717338
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti304Q → H in BAC26642 (PubMed:16141072).Curated1
Sequence conflicti1009I → V in BAC26642 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030871470 – 471MF → TC in isoform 2. 1 Publication2
Alternative sequenceiVSP_030872472 – 1614Missing in isoform 2. 1 PublicationAdd BLAST1143

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029849 mRNA. Translation: BAC26642.1.
AK133181 mRNA. Translation: BAE21546.1.
BC138448 mRNA. Translation: AAI38449.1.
CCDSiCCDS35756.1. [Q8C0T9-1]
RefSeqiNP_766617.2. NM_173029.3. [Q8C0T9-1]
XP_006496930.1. XM_006496867.1. [Q8C0T9-1]
XP_017176545.1. XM_017321056.1. [Q8C0T9-1]
UniGeneiMm.66952.

Genome annotation databases

EnsembliENSMUST00000027852; ENSMUSP00000027852; ENSMUSG00000026567. [Q8C0T9-1]
ENSMUST00000148550; ENSMUSP00000137959; ENSMUSG00000026567. [Q8C0T9-2]
GeneIDi271639.
KEGGimmu:271639.
UCSCiuc007djf.1. mouse. [Q8C0T9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029849 mRNA. Translation: BAC26642.1.
AK133181 mRNA. Translation: BAE21546.1.
BC138448 mRNA. Translation: AAI38449.1.
CCDSiCCDS35756.1. [Q8C0T9-1]
RefSeqiNP_766617.2. NM_173029.3. [Q8C0T9-1]
XP_006496930.1. XM_006496867.1. [Q8C0T9-1]
XP_017176545.1. XM_017321056.1. [Q8C0T9-1]
UniGeneiMm.66952.

3D structure databases

ProteinModelPortaliQ8C0T9.
SMRiQ8C0T9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60950N.
STRINGi10090.ENSMUSP00000027852.

PTM databases

iPTMnetiQ8C0T9.
PhosphoSitePlusiQ8C0T9.

Proteomic databases

PaxDbiQ8C0T9.
PRIDEiQ8C0T9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027852; ENSMUSP00000027852; ENSMUSG00000026567. [Q8C0T9-1]
ENSMUST00000148550; ENSMUSP00000137959; ENSMUSG00000026567. [Q8C0T9-2]
GeneIDi271639.
KEGGimmu:271639.
UCSCiuc007djf.1. mouse. [Q8C0T9-1]

Organism-specific databases

CTDi55811.
MGIiMGI:2660854. Adcy10.

Phylogenomic databases

eggNOGiENOG410IEPA. Eukaryota.
ENOG410ZFMC. LUCA.
GeneTreeiENSGT00390000001322.
HOGENOMiHOG000060149.
HOVERGENiHBG103835.
InParanoidiQ8C0T9.
KOiK11265.
OMAiPLESCQC.
OrthoDBiEOG091G00BV.
TreeFamiTF329284.

Enzyme and pathway databases

BRENDAi4.6.1.1. 3474.
ReactomeiR-MMU-5610787. Hedgehog 'off' state.

Miscellaneous databases

PROiQ8C0T9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026567.
CleanExiMM_ADCY10.
ExpressionAtlasiQ8C0T9. baseline and differential.
GenevisibleiQ8C0T9. MM.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR029787. Nucleotide_cyclase.
IPR027417. P-loop_NTPase.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55073. SSF55073. 2 hits.
PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADCYA_MOUSE
AccessioniPrimary (citable) accession number: Q8C0T9
Secondary accession number(s): B2RRJ9, Q3V0F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.