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Q8C0T9

- ADCYA_MOUSE

UniProt

Q8C0T9 - ADCYA_MOUSE

Protein

Adenylate cyclase type 10

Gene

Adcy10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor.2 Publications

    Catalytic activityi

    ATP = 3',5'-cyclic AMP + diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit.By similarity

    Enzyme regulationi

    Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Magnesium 1PROSITE-ProRule annotation
    Metal bindingi47 – 471Magnesium 2PROSITE-ProRule annotation
    Metal bindingi48 – 481Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation
    Metal bindingi99 – 991Magnesium 1PROSITE-ProRule annotation
    Metal bindingi99 – 991Magnesium 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. adenylate cyclase activity Source: MGI
    2. ATP binding Source: UniProtKB-KW
    3. magnesium ion binding Source: InterPro
    4. manganese ion binding Source: Ensembl

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. positive regulation of apoptotic process Source: Ensembl
    3. spermatogenesis Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    cAMP biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenylate cyclase type 10 (EC:4.6.1.1)
    Alternative name(s):
    Germ cell soluble adenylyl cyclase
    Short name:
    sAC
    Testicular soluble adenylyl cyclase
    Gene namesi
    Name:Adcy10
    Synonyms:Sac, Sacy
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2660854. Adcy10.

    Subcellular locationi

    Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Cytoplasmcytoskeleton 1 Publication. Cytoplasmperinuclear region 1 Publication
    Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin By similarity.By similarity

    GO - Cellular componenti

    1. apical part of cell Source: Ensembl
    2. axon Source: Ensembl
    3. basal part of cell Source: Ensembl
    4. cytoplasm Source: MGI
    5. cytoskeleton Source: UniProtKB-SubCell
    6. cytosol Source: Ensembl
    7. dendrite Source: Ensembl
    8. growth cone Source: Ensembl
    9. membrane Source: MGI
    10. neuronal cell body Source: Ensembl
    11. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    12. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are infertile because of a severe sperm-motility defect.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16141614Adenylate cyclase type 10PRO_0000317102Add
    BLAST

    Proteomic databases

    PRIDEiQ8C0T9.

    PTM databases

    PhosphoSiteiQ8C0T9.

    Expressioni

    Tissue specificityi

    Expressed in testis.1 Publication

    Gene expression databases

    BgeeiQ8C0T9.
    CleanExiMM_ADCY10.
    GenevestigatoriQ8C0T9.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60950N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8C0T9.
    SMRiQ8C0T9. Positions 1-467.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 179138Guanylate cyclase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini293 – 418126Guanylate cyclase 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi909 – 9124Poly-Glu

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 2 guanylate cyclase domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2114.
    GeneTreeiENSGT00390000001322.
    HOGENOMiHOG000060149.
    HOVERGENiHBG103835.
    InParanoidiB2RRJ9.
    KOiK11265.
    OMAiTCDSVTY.
    OrthoDBiEOG780RM3.
    TreeFamiTF329284.

    Family and domain databases

    Gene3Di3.30.70.1230. 2 hits.
    InterProiIPR001054. A/G_cyclase.
    IPR016577. Adenylate_cylcase_typ10.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00211. Guanylate_cyc. 2 hits.
    [Graphical view]
    PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF55073. SSF55073. 2 hits.
    PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8C0T9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG     50
    FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA 100
    LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEAEEGLD IRVKIGLAAG 150
    HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS 200
    MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF TKCMGFMDYY PSGDHKNFLR 250
    LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM 300
    FKEQDKVEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF 350
    PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV 400
    RHEYTVIGQK VNIAARMMMY YPGIVSCDSV TYDGSNLPAY FFKELPKKVM 450
    KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM 500
    KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS 550
    FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD EKFHCLLNDI 600
    FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LAQTVREERI IFIIDEAQFV 650
    DGTSWAFIEK LIRSMPIFIV MSLAPFSEVP CAAANAIMKN RNTTYITLGT 700
    MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRV 750
    LLFQQAETEQ KTNVTWNNMF KHSVRPTDDM QLFTSISEGQ KEVCYLVSGV 800
    RLNNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP 850
    CWNMKMMIKA LATLVESNVF NCFRSSKDLQ LALKQNVPTF EVHYRSLALK 900
    LKEGLTYGEE EELREMEGEV VECRILRFCR PIMQKTAYEL WLKDQKKVLH 950
    LKCARFLEES AHRCNHCRNV DFIPYHHFIV DIRLNTLDMD TVKRMVTSQG 1000
    FKIDEEEAIF SKSELPRKYK FPENLSITEI REKILHFFDN VILKMKSSPN 1050
    DIIPLESCQC KELLQIVILP LAQHFVALEE NNKALYYFLE LASAYLILGD 1100
    NYNAYMYLGE GERLLKSLTN EDSWSQTFEY ATFYSLKAEV CFNMGQMVLA 1150
    KKMLRKALKL LNRMFPCNLL TLTFQMHVEK NRLSHFMNQH TQEGSVPGKK 1200
    LAQLYLQASC FSLLWRIYSL NFFFHYKYYG HLAAMMEMNT SLETQNDFQI 1250
    IKAYLDFSLY HHLAGYQGVW FKYEILVMEQ LLNLPLKGEA IEIMAYTADT 1300
    LGHIKFLMGH LDLAIELGSR AHRMWSLLRN PNKYQMVLCR LSKPLFLKSR 1350
    YKHLVQVLGW LWDLSVTEED IFSKAFFYFV CLDIMLYSGF IYRTFEECLE 1400
    FIHHNEDNRI LKFQSGLLLG LYSCIAVWYA RLQEWDNFNK FSDRAKHLVT 1450
    RRTPTVLYYE GISRYMEGQV LHLQKQIEEQ AENAQDSGVE ILKALETLVA 1500
    QNTTGPVFYP RLYHLMAYVC ILMGDGHSCD FFLNTALELS ETHGNLLEKC 1550
    WLSMSKEWWY SASELTGDQW LQTVLSLPSW DKIVSGKGGQ RKRSWSWFCP 1600
    PNFSMVSWSQ PQCA 1614
    Length:1,614
    Mass (Da):186,412
    Last modified:July 27, 2011 - v2
    Checksum:iAE56433825277126
    GO
    Isoform 2 (identifier: Q8C0T9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         470-471: MF → TC
         472-1614: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:471
    Mass (Da):52,952
    Checksum:iBE78B5B846717338
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti304 – 3041Q → H in BAC26642. (PubMed:16141072)Curated
    Sequence conflicti1009 – 10091I → V in BAC26642. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei470 – 4712MF → TC in isoform 2. 1 PublicationVSP_030871
    Alternative sequencei472 – 16141143Missing in isoform 2. 1 PublicationVSP_030872Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK029849 mRNA. Translation: BAC26642.1.
    AK133181 mRNA. Translation: BAE21546.1.
    BC138448 mRNA. Translation: AAI38449.1.
    CCDSiCCDS35756.1. [Q8C0T9-1]
    RefSeqiNP_766617.2. NM_173029.3. [Q8C0T9-1]
    XP_006496930.1. XM_006496867.1. [Q8C0T9-1]
    UniGeneiMm.66952.

    Genome annotation databases

    EnsembliENSMUST00000027852; ENSMUSP00000027852; ENSMUSG00000026567. [Q8C0T9-1]
    ENSMUST00000148550; ENSMUSP00000137959; ENSMUSG00000026567. [Q8C0T9-2]
    GeneIDi271639.
    KEGGimmu:271639.
    UCSCiuc007djf.1. mouse. [Q8C0T9-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK029849 mRNA. Translation: BAC26642.1 .
    AK133181 mRNA. Translation: BAE21546.1 .
    BC138448 mRNA. Translation: AAI38449.1 .
    CCDSi CCDS35756.1. [Q8C0T9-1 ]
    RefSeqi NP_766617.2. NM_173029.3. [Q8C0T9-1 ]
    XP_006496930.1. XM_006496867.1. [Q8C0T9-1 ]
    UniGenei Mm.66952.

    3D structure databases

    ProteinModelPortali Q8C0T9.
    SMRi Q8C0T9. Positions 1-467.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60950N.

    PTM databases

    PhosphoSitei Q8C0T9.

    Proteomic databases

    PRIDEi Q8C0T9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027852 ; ENSMUSP00000027852 ; ENSMUSG00000026567 . [Q8C0T9-1 ]
    ENSMUST00000148550 ; ENSMUSP00000137959 ; ENSMUSG00000026567 . [Q8C0T9-2 ]
    GeneIDi 271639.
    KEGGi mmu:271639.
    UCSCi uc007djf.1. mouse. [Q8C0T9-1 ]

    Organism-specific databases

    CTDi 55811.
    MGIi MGI:2660854. Adcy10.

    Phylogenomic databases

    eggNOGi COG2114.
    GeneTreei ENSGT00390000001322.
    HOGENOMi HOG000060149.
    HOVERGENi HBG103835.
    InParanoidi B2RRJ9.
    KOi K11265.
    OMAi TCDSVTY.
    OrthoDBi EOG780RM3.
    TreeFami TF329284.

    Miscellaneous databases

    ChiTaRSi ADCY10. mouse.
    NextBioi 393482.
    PROi Q8C0T9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8C0T9.
    CleanExi MM_ADCY10.
    Genevestigatori Q8C0T9.

    Family and domain databases

    Gene3Di 3.30.70.1230. 2 hits.
    InterProi IPR001054. A/G_cyclase.
    IPR016577. Adenylate_cylcase_typ10.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00211. Guanylate_cyc. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF55073. SSF55073. 2 hits.
    PROSITEi PS50125. GUANYLATE_CYCLASE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase."
      Jaiswal B.S., Conti M.
      J. Biol. Chem. 276:31698-31708(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
    4. "Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains."
      Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J.
      FASEB J. 17:82-84(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "The 'soluble' adenylyl cyclase in sperm mediates multiple signaling events required for fertilization."
      Hess K.C., Jones B.H., Marquez B., Chen Y., Ord T.S., Kamenetsky M., Miyamoto C., Zippin J.H., Kopf G.S., Suarez S.S., Levin L.R., Williams C.J., Buck J., Moss S.B.
      Dev. Cell 9:249-259(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiADCYA_MOUSE
    AccessioniPrimary (citable) accession number: Q8C0T9
    Secondary accession number(s): B2RRJ9, Q3V0F8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3