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Q8C0T9

- ADCYA_MOUSE

UniProt

Q8C0T9 - ADCYA_MOUSE

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Protein

Adenylate cyclase type 10

Gene

Adcy10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor.2 Publications

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Magnesium 1PROSITE-ProRule annotation
Metal bindingi47 – 471Magnesium 2PROSITE-ProRule annotation
Metal bindingi48 – 481Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi99 – 991Magnesium 1PROSITE-ProRule annotation
Metal bindingi99 – 991Magnesium 2PROSITE-ProRule annotation

GO - Molecular functioni

  1. adenylate cyclase activity Source: MGI
  2. ATP binding Source: UniProtKB-KW
  3. magnesium ion binding Source: InterPro
  4. manganese ion binding Source: Ensembl

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. positive regulation of apoptotic process Source: Ensembl
  3. spermatogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cAMP biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_269412. Hedgehog 'off' state.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 10 (EC:4.6.1.1)
Alternative name(s):
Germ cell soluble adenylyl cyclase
Short name:
sAC
Testicular soluble adenylyl cyclase
Gene namesi
Name:Adcy10
Synonyms:Sac, Sacy
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2660854. Adcy10.

Subcellular locationi

Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Cytoplasmcytoskeleton 1 Publication. Cytoplasmperinuclear region 1 Publication
Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin (By similarity).By similarity

GO - Cellular componenti

  1. apical part of cell Source: Ensembl
  2. axon Source: Ensembl
  3. basal part of cell Source: Ensembl
  4. cytoplasm Source: MGI
  5. cytoskeleton Source: UniProtKB-KW
  6. cytosol Source: Ensembl
  7. dendrite Source: Ensembl
  8. growth cone Source: Ensembl
  9. membrane Source: MGI
  10. neuronal cell body Source: Ensembl
  11. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are infertile because of a severe sperm-motility defect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16141614Adenylate cyclase type 10PRO_0000317102Add
BLAST

Proteomic databases

PRIDEiQ8C0T9.

PTM databases

PhosphoSiteiQ8C0T9.

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Gene expression databases

BgeeiQ8C0T9.
CleanExiMM_ADCY10.
ExpressionAtlasiQ8C0T9. baseline and differential.
GenevestigatoriQ8C0T9.

Interactioni

Protein-protein interaction databases

DIPiDIP-60950N.

Structurei

3D structure databases

ProteinModelPortaliQ8C0T9.
SMRiQ8C0T9. Positions 1-467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 179138Guanylate cyclase 1PROSITE-ProRule annotationAdd
BLAST
Domaini293 – 418126Guanylate cyclase 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi909 – 9124Poly-Glu

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 2 guanylate cyclase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00390000001322.
HOGENOMiHOG000060149.
HOVERGENiHBG103835.
InParanoidiQ8C0T9.
KOiK11265.
OMAiTCDSVTY.
OrthoDBiEOG780RM3.
TreeFamiTF329284.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR029787. Nucleotide_cyclase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55073. SSF55073. 2 hits.
PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8C0T9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG
60 70 80 90 100
FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA
110 120 130 140 150
LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEAEEGLD IRVKIGLAAG
160 170 180 190 200
HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS
210 220 230 240 250
MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF TKCMGFMDYY PSGDHKNFLR
260 270 280 290 300
LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM
310 320 330 340 350
FKEQDKVEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF
360 370 380 390 400
PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV
410 420 430 440 450
RHEYTVIGQK VNIAARMMMY YPGIVSCDSV TYDGSNLPAY FFKELPKKVM
460 470 480 490 500
KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM
510 520 530 540 550
KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS
560 570 580 590 600
FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD EKFHCLLNDI
610 620 630 640 650
FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LAQTVREERI IFIIDEAQFV
660 670 680 690 700
DGTSWAFIEK LIRSMPIFIV MSLAPFSEVP CAAANAIMKN RNTTYITLGT
710 720 730 740 750
MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRV
760 770 780 790 800
LLFQQAETEQ KTNVTWNNMF KHSVRPTDDM QLFTSISEGQ KEVCYLVSGV
810 820 830 840 850
RLNNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP
860 870 880 890 900
CWNMKMMIKA LATLVESNVF NCFRSSKDLQ LALKQNVPTF EVHYRSLALK
910 920 930 940 950
LKEGLTYGEE EELREMEGEV VECRILRFCR PIMQKTAYEL WLKDQKKVLH
960 970 980 990 1000
LKCARFLEES AHRCNHCRNV DFIPYHHFIV DIRLNTLDMD TVKRMVTSQG
1010 1020 1030 1040 1050
FKIDEEEAIF SKSELPRKYK FPENLSITEI REKILHFFDN VILKMKSSPN
1060 1070 1080 1090 1100
DIIPLESCQC KELLQIVILP LAQHFVALEE NNKALYYFLE LASAYLILGD
1110 1120 1130 1140 1150
NYNAYMYLGE GERLLKSLTN EDSWSQTFEY ATFYSLKAEV CFNMGQMVLA
1160 1170 1180 1190 1200
KKMLRKALKL LNRMFPCNLL TLTFQMHVEK NRLSHFMNQH TQEGSVPGKK
1210 1220 1230 1240 1250
LAQLYLQASC FSLLWRIYSL NFFFHYKYYG HLAAMMEMNT SLETQNDFQI
1260 1270 1280 1290 1300
IKAYLDFSLY HHLAGYQGVW FKYEILVMEQ LLNLPLKGEA IEIMAYTADT
1310 1320 1330 1340 1350
LGHIKFLMGH LDLAIELGSR AHRMWSLLRN PNKYQMVLCR LSKPLFLKSR
1360 1370 1380 1390 1400
YKHLVQVLGW LWDLSVTEED IFSKAFFYFV CLDIMLYSGF IYRTFEECLE
1410 1420 1430 1440 1450
FIHHNEDNRI LKFQSGLLLG LYSCIAVWYA RLQEWDNFNK FSDRAKHLVT
1460 1470 1480 1490 1500
RRTPTVLYYE GISRYMEGQV LHLQKQIEEQ AENAQDSGVE ILKALETLVA
1510 1520 1530 1540 1550
QNTTGPVFYP RLYHLMAYVC ILMGDGHSCD FFLNTALELS ETHGNLLEKC
1560 1570 1580 1590 1600
WLSMSKEWWY SASELTGDQW LQTVLSLPSW DKIVSGKGGQ RKRSWSWFCP
1610
PNFSMVSWSQ PQCA
Length:1,614
Mass (Da):186,412
Last modified:July 27, 2011 - v2
Checksum:iAE56433825277126
GO
Isoform 2 (identifier: Q8C0T9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-471: MF → TC
     472-1614: Missing.

Note: No experimental confirmation available.

Show »
Length:471
Mass (Da):52,952
Checksum:iBE78B5B846717338
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti304 – 3041Q → H in BAC26642. (PubMed:16141072)Curated
Sequence conflicti1009 – 10091I → V in BAC26642. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei470 – 4712MF → TC in isoform 2. 1 PublicationVSP_030871
Alternative sequencei472 – 16141143Missing in isoform 2. 1 PublicationVSP_030872Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029849 mRNA. Translation: BAC26642.1.
AK133181 mRNA. Translation: BAE21546.1.
BC138448 mRNA. Translation: AAI38449.1.
CCDSiCCDS35756.1. [Q8C0T9-1]
RefSeqiNP_766617.2. NM_173029.3. [Q8C0T9-1]
XP_006496930.1. XM_006496867.1. [Q8C0T9-1]
UniGeneiMm.66952.

Genome annotation databases

EnsembliENSMUST00000027852; ENSMUSP00000027852; ENSMUSG00000026567. [Q8C0T9-1]
ENSMUST00000148550; ENSMUSP00000137959; ENSMUSG00000026567. [Q8C0T9-2]
GeneIDi271639.
KEGGimmu:271639.
UCSCiuc007djf.1. mouse. [Q8C0T9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029849 mRNA. Translation: BAC26642.1 .
AK133181 mRNA. Translation: BAE21546.1 .
BC138448 mRNA. Translation: AAI38449.1 .
CCDSi CCDS35756.1. [Q8C0T9-1 ]
RefSeqi NP_766617.2. NM_173029.3. [Q8C0T9-1 ]
XP_006496930.1. XM_006496867.1. [Q8C0T9-1 ]
UniGenei Mm.66952.

3D structure databases

ProteinModelPortali Q8C0T9.
SMRi Q8C0T9. Positions 1-467.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60950N.

PTM databases

PhosphoSitei Q8C0T9.

Proteomic databases

PRIDEi Q8C0T9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027852 ; ENSMUSP00000027852 ; ENSMUSG00000026567 . [Q8C0T9-1 ]
ENSMUST00000148550 ; ENSMUSP00000137959 ; ENSMUSG00000026567 . [Q8C0T9-2 ]
GeneIDi 271639.
KEGGi mmu:271639.
UCSCi uc007djf.1. mouse. [Q8C0T9-1 ]

Organism-specific databases

CTDi 55811.
MGIi MGI:2660854. Adcy10.

Phylogenomic databases

eggNOGi COG2114.
GeneTreei ENSGT00390000001322.
HOGENOMi HOG000060149.
HOVERGENi HBG103835.
InParanoidi Q8C0T9.
KOi K11265.
OMAi TCDSVTY.
OrthoDBi EOG780RM3.
TreeFami TF329284.

Enzyme and pathway databases

Reactomei REACT_269412. Hedgehog 'off' state.

Miscellaneous databases

NextBioi 393482.
PROi Q8C0T9.
SOURCEi Search...

Gene expression databases

Bgeei Q8C0T9.
CleanExi MM_ADCY10.
ExpressionAtlasi Q8C0T9. baseline and differential.
Genevestigatori Q8C0T9.

Family and domain databases

Gene3Di 3.30.70.1230. 2 hits.
InterProi IPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR029787. Nucleotide_cyclase.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00211. Guanylate_cyc. 2 hits.
[Graphical view ]
PIRSFi PIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTi SM00044. CYCc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF55073. SSF55073. 2 hits.
PROSITEi PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase."
    Jaiswal B.S., Conti M.
    J. Biol. Chem. 276:31698-31708(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
  4. "Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains."
    Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J.
    FASEB J. 17:82-84(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "The 'soluble' adenylyl cyclase in sperm mediates multiple signaling events required for fertilization."
    Hess K.C., Jones B.H., Marquez B., Chen Y., Ord T.S., Kamenetsky M., Miyamoto C., Zippin J.H., Kopf G.S., Suarez S.S., Levin L.R., Williams C.J., Buck J., Moss S.B.
    Dev. Cell 9:249-259(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiADCYA_MOUSE
AccessioniPrimary (citable) accession number: Q8C0T9
Secondary accession number(s): B2RRJ9, Q3V0F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3