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Q8C0T9

- ADCYA_MOUSE

UniProt

Q8C0T9 - ADCYA_MOUSE

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Protein
Adenylate cyclase type 10
Gene
Adcy10, Sac, Sacy
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Soluble adenylyl cyclase that has a critical role in mammalian spermatogenesis. Produces the cAMP which mediates in part the cAMP-responsive nuclear factors indispensable for maturation of sperm in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization. May be the bicarbonate sensor.2 Publications

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Binds 2 magnesium ions per subunit By similarity.

Enzyme regulationi

Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate while in the presence of manganese ions, the enzyme is inhibited by bicarbonate. In the absence of magnesium and bicarbonate, the enzyme is weakly activated by calcium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Magnesium 1 By similarity
Metal bindingi47 – 471Magnesium 2 By similarity
Metal bindingi48 – 481Magnesium 2; via carbonyl oxygen By similarity
Metal bindingi99 – 991Magnesium 1 By similarity
Metal bindingi99 – 991Magnesium 2 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. adenylate cyclase activity Source: MGI
  3. magnesium ion binding Source: InterPro
  4. manganese ion binding Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. positive regulation of apoptotic process Source: Ensembl
  3. spermatogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cAMP biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 10 (EC:4.6.1.1)
Alternative name(s):
Germ cell soluble adenylyl cyclase
Short name:
sAC
Testicular soluble adenylyl cyclase
Gene namesi
Name:Adcy10
Synonyms:Sac, Sacy
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2660854. Adcy10.

Subcellular locationi

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton. Cytoplasmperinuclear region
Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane-associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin By similarity.1 Publication

GO - Cellular componenti

  1. apical part of cell Source: Ensembl
  2. axon Source: Ensembl
  3. basal part of cell Source: Ensembl
  4. cytoplasm Source: MGI
  5. cytoskeleton Source: UniProtKB-SubCell
  6. cytosol Source: Ensembl
  7. dendrite Source: Ensembl
  8. growth cone Source: Ensembl
  9. membrane Source: MGI
  10. neuronal cell body Source: Ensembl
  11. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  12. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are infertile because of a severe sperm-motility defect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16141614Adenylate cyclase type 10
PRO_0000317102Add
BLAST

Proteomic databases

PRIDEiQ8C0T9.

PTM databases

PhosphoSiteiQ8C0T9.

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Gene expression databases

BgeeiQ8C0T9.
CleanExiMM_ADCY10.
GenevestigatoriQ8C0T9.

Structurei

3D structure databases

ProteinModelPortaliQ8C0T9.
SMRiQ8C0T9. Positions 1-467.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 179138Guanylate cyclase 1
Add
BLAST
Domaini293 – 418126Guanylate cyclase 2
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi909 – 9124Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00390000001322.
HOGENOMiHOG000060149.
HOVERGENiHBG103835.
InParanoidiB2RRJ9.
KOiK11265.
OMAiTCDSVTY.
OrthoDBiEOG780RM3.
TreeFamiTF329284.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55073. SSF55073. 2 hits.
PROSITEiPS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8C0T9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG     50
FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA 100
LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEAEEGLD IRVKIGLAAG 150
HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS 200
MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF TKCMGFMDYY PSGDHKNFLR 250
LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM 300
FKEQDKVEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF 350
PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV 400
RHEYTVIGQK VNIAARMMMY YPGIVSCDSV TYDGSNLPAY FFKELPKKVM 450
KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM 500
KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS 550
FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD EKFHCLLNDI 600
FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LAQTVREERI IFIIDEAQFV 650
DGTSWAFIEK LIRSMPIFIV MSLAPFSEVP CAAANAIMKN RNTTYITLGT 700
MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRV 750
LLFQQAETEQ KTNVTWNNMF KHSVRPTDDM QLFTSISEGQ KEVCYLVSGV 800
RLNNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP 850
CWNMKMMIKA LATLVESNVF NCFRSSKDLQ LALKQNVPTF EVHYRSLALK 900
LKEGLTYGEE EELREMEGEV VECRILRFCR PIMQKTAYEL WLKDQKKVLH 950
LKCARFLEES AHRCNHCRNV DFIPYHHFIV DIRLNTLDMD TVKRMVTSQG 1000
FKIDEEEAIF SKSELPRKYK FPENLSITEI REKILHFFDN VILKMKSSPN 1050
DIIPLESCQC KELLQIVILP LAQHFVALEE NNKALYYFLE LASAYLILGD 1100
NYNAYMYLGE GERLLKSLTN EDSWSQTFEY ATFYSLKAEV CFNMGQMVLA 1150
KKMLRKALKL LNRMFPCNLL TLTFQMHVEK NRLSHFMNQH TQEGSVPGKK 1200
LAQLYLQASC FSLLWRIYSL NFFFHYKYYG HLAAMMEMNT SLETQNDFQI 1250
IKAYLDFSLY HHLAGYQGVW FKYEILVMEQ LLNLPLKGEA IEIMAYTADT 1300
LGHIKFLMGH LDLAIELGSR AHRMWSLLRN PNKYQMVLCR LSKPLFLKSR 1350
YKHLVQVLGW LWDLSVTEED IFSKAFFYFV CLDIMLYSGF IYRTFEECLE 1400
FIHHNEDNRI LKFQSGLLLG LYSCIAVWYA RLQEWDNFNK FSDRAKHLVT 1450
RRTPTVLYYE GISRYMEGQV LHLQKQIEEQ AENAQDSGVE ILKALETLVA 1500
QNTTGPVFYP RLYHLMAYVC ILMGDGHSCD FFLNTALELS ETHGNLLEKC 1550
WLSMSKEWWY SASELTGDQW LQTVLSLPSW DKIVSGKGGQ RKRSWSWFCP 1600
PNFSMVSWSQ PQCA 1614
Length:1,614
Mass (Da):186,412
Last modified:July 27, 2011 - v2
Checksum:iAE56433825277126
GO
Isoform 2 (identifier: Q8C0T9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-471: MF → TC
     472-1614: Missing.

Note: No experimental confirmation available.

Show »
Length:471
Mass (Da):52,952
Checksum:iBE78B5B846717338
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei470 – 4712MF → TC in isoform 2.
VSP_030871
Alternative sequencei472 – 16141143Missing in isoform 2.
VSP_030872Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti304 – 3041Q → H in BAC26642. 1 Publication
Sequence conflicti1009 – 10091I → V in BAC26642. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK029849 mRNA. Translation: BAC26642.1.
AK133181 mRNA. Translation: BAE21546.1.
BC138448 mRNA. Translation: AAI38449.1.
CCDSiCCDS35756.1. [Q8C0T9-1]
RefSeqiNP_766617.2. NM_173029.3. [Q8C0T9-1]
XP_006496930.1. XM_006496867.1. [Q8C0T9-1]
UniGeneiMm.66952.

Genome annotation databases

EnsembliENSMUST00000027852; ENSMUSP00000027852; ENSMUSG00000026567. [Q8C0T9-1]
ENSMUST00000148550; ENSMUSP00000137959; ENSMUSG00000026567. [Q8C0T9-2]
GeneIDi271639.
KEGGimmu:271639.
UCSCiuc007djf.1. mouse. [Q8C0T9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK029849 mRNA. Translation: BAC26642.1 .
AK133181 mRNA. Translation: BAE21546.1 .
BC138448 mRNA. Translation: AAI38449.1 .
CCDSi CCDS35756.1. [Q8C0T9-1 ]
RefSeqi NP_766617.2. NM_173029.3. [Q8C0T9-1 ]
XP_006496930.1. XM_006496867.1. [Q8C0T9-1 ]
UniGenei Mm.66952.

3D structure databases

ProteinModelPortali Q8C0T9.
SMRi Q8C0T9. Positions 1-467.
ModBasei Search...

PTM databases

PhosphoSitei Q8C0T9.

Proteomic databases

PRIDEi Q8C0T9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027852 ; ENSMUSP00000027852 ; ENSMUSG00000026567 . [Q8C0T9-1 ]
ENSMUST00000148550 ; ENSMUSP00000137959 ; ENSMUSG00000026567 . [Q8C0T9-2 ]
GeneIDi 271639.
KEGGi mmu:271639.
UCSCi uc007djf.1. mouse. [Q8C0T9-1 ]

Organism-specific databases

CTDi 55811.
MGIi MGI:2660854. Adcy10.

Phylogenomic databases

eggNOGi COG2114.
GeneTreei ENSGT00390000001322.
HOGENOMi HOG000060149.
HOVERGENi HBG103835.
InParanoidi B2RRJ9.
KOi K11265.
OMAi TCDSVTY.
OrthoDBi EOG780RM3.
TreeFami TF329284.

Miscellaneous databases

ChiTaRSi ADCY10. mouse.
NextBioi 393482.
PROi Q8C0T9.
SOURCEi Search...

Gene expression databases

Bgeei Q8C0T9.
CleanExi MM_ADCY10.
Genevestigatori Q8C0T9.

Family and domain databases

Gene3Di 3.30.70.1230. 2 hits.
InterProi IPR001054. A/G_cyclase.
IPR016577. Adenylate_cylcase_typ10.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00211. Guanylate_cyc. 2 hits.
[Graphical view ]
PIRSFi PIRSF011131. Soluble_adenylyl_cyclase. 1 hit.
SMARTi SM00044. CYCc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF55073. SSF55073. 2 hits.
PROSITEi PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Identification and functional analysis of splice variants of the germ cell soluble adenylyl cyclase."
    Jaiswal B.S., Conti M.
    J. Biol. Chem. 276:31698-31708(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
  4. "Compartmentalization of bicarbonate-sensitive adenylyl cyclase in distinct signaling microdomains."
    Zippin J.H., Chen Y., Nahirney P., Kamenetsky M., Wuttke M.S., Fischman D.A., Levin L.R., Buck J.
    FASEB J. 17:82-84(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "The 'soluble' adenylyl cyclase in sperm mediates multiple signaling events required for fertilization."
    Hess K.C., Jones B.H., Marquez B., Chen Y., Ord T.S., Kamenetsky M., Miyamoto C., Zippin J.H., Kopf G.S., Suarez S.S., Levin L.R., Williams C.J., Buck J., Moss S.B.
    Dev. Cell 9:249-259(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiADCYA_MOUSE
AccessioniPrimary (citable) accession number: Q8C0T9
Secondary accession number(s): B2RRJ9, Q3V0F8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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