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Protein

Adenylate cyclase type 10

Gene

Adcy10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the signaling molecule cAMP. May function as sensor that mediates responses to changes in cellular bicarbonate and CO2 levels (By similarity). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (PubMed:14976244, PubMed:16054031). Involved in ciliary beat regulation (By similarity).By similarity2 Publications

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).By similarity

Enzyme regulationi

Activated by manganese or magnesium ions. In the presence of magnesium ions, the enzyme is activated by bicarbonate. Calcium mildly increases the enzyme activity, also in the presence of magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Magnesium 1PROSITE-ProRule annotation1
Metal bindingi47Magnesium 2PROSITE-ProRule annotation1
Metal bindingi48Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei95BicarbonateBy similarity1
Metal bindingi99Magnesium 1PROSITE-ProRule annotation1
Metal bindingi99Magnesium 2PROSITE-ProRule annotation1
Binding sitei99ATPBy similarity1
Binding sitei144ATPBy similarity1
Binding sitei167Bicarbonate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei176BicarbonateBy similarity1
Binding sitei337Bicarbonate; via amide nitrogenBy similarity1
Binding sitei406ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 52ATPBy similarity6
Nucleotide bindingi412 – 416ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processcAMP biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.1 3474
ReactomeiR-MMU-5610787 Hedgehog 'off' state

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 10 (EC:4.6.1.1)
Alternative name(s):
Germ cell soluble adenylyl cyclase
Short name:
sAC
Testicular soluble adenylyl cyclase
Gene namesi
Name:Adcy10
Synonyms:Sac, Sacy
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2660854 Adcy10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are infertile because of a severe sperm-motility defect.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003171021 – 1614Adenylate cyclase type 10Add BLAST1614

Proteomic databases

PaxDbiQ8C0T9
PRIDEiQ8C0T9

PTM databases

iPTMnetiQ8C0T9
PhosphoSitePlusiQ8C0T9

Expressioni

Tissue specificityi

Expressed in testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000026567
CleanExiMM_ADCY10
ExpressionAtlasiQ8C0T9 baseline and differential
GenevisibleiQ8C0T9 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Slc9c1Q6UJY22EBI-15639026,EBI-15639080

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60950N
IntActiQ8C0T9, 1 interactor
STRINGi10090.ENSMUSP00000027852

Structurei

3D structure databases

ProteinModelPortaliQ8C0T9
SMRiQ8C0T9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 179Guanylate cyclase 1PROSITE-ProRule annotationAdd BLAST138
Domaini293 – 418Guanylate cyclase 2PROSITE-ProRule annotationAdd BLAST126

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi909 – 912Poly-Glu4

Domaini

The N-terminal guanylate cyclase domains are required for enzyme activity. Fragments containing the first 470 amino acid residues are fully active.By similarity

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IEPA Eukaryota
ENOG410ZFMC LUCA
GeneTreeiENSGT00390000001322
HOGENOMiHOG000060149
HOVERGENiHBG103835
InParanoidiQ8C0T9
KOiK11265
OMAiMYYPGIV
OrthoDBiEOG091G00BV
TreeFamiTF329284

Family and domain databases

Gene3Di3.30.70.1230, 2 hits
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR016577 Adenylate_cyclase_typ10
IPR029787 Nucleotide_cyclase
IPR027417 P-loop_NTPase
IPR011990 TPR-like_helical_dom_sf
PfamiView protein in Pfam
PF00211 Guanylate_cyc, 2 hits
PIRSFiPIRSF011131 Soluble_adenylyl_cyclase, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF48452 SSF48452, 2 hits
SSF52540 SSF52540, 1 hit
SSF55073 SSF55073, 2 hits
PROSITEiView protein in PROSITE
PS50125 GUANYLATE_CYCLASE_2, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C0T9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSARRQELQD RAIVKIAAHL PDLIVYGDFS PERPSVKCFD GVLMFVDISG
60 70 80 90 100
FTAMTEKFST AMYMDRGAEQ LVEILNYYIS AIVEKVLIFG GDILKFAGDA
110 120 130 140 150
LLALWKVERK QLKNIITVVI KCSLEIHGLF EAKEAEEGLD IRVKIGLAAG
160 170 180 190 200
HITMLVFGDE TRNYFLVIGQ AVDDVRLAQN MAQMNDVILS PNCWQLCDRS
210 220 230 240 250
MIEIERIPDQ RAVKVSFLKP PPTFNFDEFF TKCMGFMDYY PSGDHKNFLR
260 270 280 290 300
LACMLESDPE LELSLQKYVM EIILKQIDDK QLRGYLSELR PVTIVFVNLM
310 320 330 340 350
FKEQDKVEVI GSAIQAACVH ITSVLKVFRG QINKVFMFDK GCSFLCVFGF
360 370 380 390 400
PGEKAPDEIT HALESAVDIF DFCSQVHKIR TVSIGVASGI VFCGIVGHTV
410 420 430 440 450
RHEYTVIGQK VNIAARMMMY YPGIVSCDSV TYDGSNLPAY FFKELPKKVM
460 470 480 490 500
KGVADPGPVY QCLGLNEKVM FGMAYLICNR YEGYPLLGRV REIDYFMSTM
510 520 530 540 550
KDFLMTNCSR VLMYEGLPGY GKSQVLMEIE YLASQHENHR AVAIALTKIS
560 570 580 590 600
FHQNFYTIQI LMANVLGLDT CKHYKERQTN LQNRVKTLLD EKFHCLLNDI
610 620 630 640 650
FHVQFPVSRE MSRMSKIRKQ KQLEALFMKI LAQTVREERI IFIIDEAQFV
660 670 680 690 700
DGTSWAFIEK LIRSMPIFIV MSLAPFSEVP CAAANAIMKN RNTTYITLGT
710 720 730 740 750
MQPQEIRDKV CVDLSVSSIP RELDSYLVEG SCGIPYYCEE LLKNLDHHRV
760 770 780 790 800
LLFQQAETEQ KTNVTWNNMF KHSVRPTDDM QLFTSISEGQ KEVCYLVSGV
810 820 830 840 850
RLNNLSPPAS LKEISLVQLD SMSLSHQMLV RCAAIIGLTF TTELLFEILP
860 870 880 890 900
CWNMKMMIKA LATLVESNVF NCFRSSKDLQ LALKQNVPTF EVHYRSLALK
910 920 930 940 950
LKEGLTYGEE EELREMEGEV VECRILRFCR PIMQKTAYEL WLKDQKKVLH
960 970 980 990 1000
LKCARFLEES AHRCNHCRNV DFIPYHHFIV DIRLNTLDMD TVKRMVTSQG
1010 1020 1030 1040 1050
FKIDEEEAIF SKSELPRKYK FPENLSITEI REKILHFFDN VILKMKSSPN
1060 1070 1080 1090 1100
DIIPLESCQC KELLQIVILP LAQHFVALEE NNKALYYFLE LASAYLILGD
1110 1120 1130 1140 1150
NYNAYMYLGE GERLLKSLTN EDSWSQTFEY ATFYSLKAEV CFNMGQMVLA
1160 1170 1180 1190 1200
KKMLRKALKL LNRMFPCNLL TLTFQMHVEK NRLSHFMNQH TQEGSVPGKK
1210 1220 1230 1240 1250
LAQLYLQASC FSLLWRIYSL NFFFHYKYYG HLAAMMEMNT SLETQNDFQI
1260 1270 1280 1290 1300
IKAYLDFSLY HHLAGYQGVW FKYEILVMEQ LLNLPLKGEA IEIMAYTADT
1310 1320 1330 1340 1350
LGHIKFLMGH LDLAIELGSR AHRMWSLLRN PNKYQMVLCR LSKPLFLKSR
1360 1370 1380 1390 1400
YKHLVQVLGW LWDLSVTEED IFSKAFFYFV CLDIMLYSGF IYRTFEECLE
1410 1420 1430 1440 1450
FIHHNEDNRI LKFQSGLLLG LYSCIAVWYA RLQEWDNFNK FSDRAKHLVT
1460 1470 1480 1490 1500
RRTPTVLYYE GISRYMEGQV LHLQKQIEEQ AENAQDSGVE ILKALETLVA
1510 1520 1530 1540 1550
QNTTGPVFYP RLYHLMAYVC ILMGDGHSCD FFLNTALELS ETHGNLLEKC
1560 1570 1580 1590 1600
WLSMSKEWWY SASELTGDQW LQTVLSLPSW DKIVSGKGGQ RKRSWSWFCP
1610
PNFSMVSWSQ PQCA
Length:1,614
Mass (Da):186,412
Last modified:July 27, 2011 - v2
Checksum:iAE56433825277126
GO
Isoform 2 (identifier: Q8C0T9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-471: MF → TC
     472-1614: Missing.

Note: No experimental confirmation available.
Show »
Length:471
Mass (Da):52,952
Checksum:iBE78B5B846717338
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti304Q → H in BAC26642 (PubMed:16141072).Curated1
Sequence conflicti1009I → V in BAC26642 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030871470 – 471MF → TC in isoform 2. 1 Publication2
Alternative sequenceiVSP_030872472 – 1614Missing in isoform 2. 1 PublicationAdd BLAST1143

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029849 mRNA Translation: BAC26642.1
AK133181 mRNA Translation: BAE21546.1
BC138448 mRNA Translation: AAI38449.1
CCDSiCCDS35756.1 [Q8C0T9-1]
RefSeqiNP_766617.2, NM_173029.3 [Q8C0T9-1]
XP_006496930.1, XM_006496867.1 [Q8C0T9-1]
XP_017176545.1, XM_017321056.1 [Q8C0T9-1]
UniGeneiMm.66952

Genome annotation databases

EnsembliENSMUST00000027852; ENSMUSP00000027852; ENSMUSG00000026567 [Q8C0T9-1]
ENSMUST00000148550; ENSMUSP00000137959; ENSMUSG00000026567 [Q8C0T9-2]
GeneIDi271639
KEGGimmu:271639
UCSCiuc007djf.1 mouse [Q8C0T9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiADCYA_MOUSE
AccessioniPrimary (citable) accession number: Q8C0T9
Secondary accession number(s): B2RRJ9, Q3V0F8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health