Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 37

Gene

Usp37

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei350NucleophilePROSITE-ProRule annotationBy similarity1
Active sitei906Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5689880 Ub-specific processing proteases

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 37 (EC:3.4.19.12By similarity)
Alternative name(s):
Deubiquitinating enzyme 37
Ubiquitin thioesterase 37
Ubiquitin-specific-processing protease 37
Gene namesi
Name:Usp37Imported
Synonyms:Kiaa1594Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442483 Usp37

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002596101 – 979Ubiquitin carboxyl-terminal hydrolase 37Add BLAST979

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei212PhosphoserineBy similarity1
Modified residuei628Phosphoserine; by CDK2By similarity1
Modified residuei650PhosphoserineCombined sources1
Modified residuei652PhosphoserineCombined sources1
Modified residuei770PhosphoserineBy similarity1

Post-translational modificationi

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the APC(CDH1) complex during late mitosis, leading to its degradation. Able to mediate auto-deubiquitination.By similarity
Phosphorylated at Ser-628 by CDK2 during G1/S phase but not during mitosis; phosphorylation at Ser-628 is required for deubiquitinase activity. Also polyubiquitinated during early G1 phase, without leading to degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8C0R0
PeptideAtlasiQ8C0R0
PRIDEiQ8C0R0

PTM databases

iPTMnetiQ8C0R0
PhosphoSitePlusiQ8C0R0

Expressioni

Gene expression databases

BgeeiENSMUSG00000033364
CleanExiMM_USP37
ExpressionAtlasiQ8C0R0 baseline and differential
GenevisibleiQ8C0R0 MM

Interactioni

Subunit structurei

Interacts with FZR1/CDH1. Interacts with CDT1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi235424, 1 interactor
STRINGi10090.ENSMUSP00000035445

Structurei

3D structure databases

ProteinModelPortaliQ8C0R0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini341 – 951USPAdd BLAST611
Domaini704 – 723UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini806 – 825UIM 2PROSITE-ProRule annotationAdd BLAST20
Domaini828 – 847UIM 3PROSITE-ProRule annotationAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 34KEN box 1By similarity3
Motifi71 – 79D-box 1By similarity9
Motifi96 – 105D-box 2By similarity10
Motifi160 – 168D-box 3By similarity9
Motifi222 – 224KEN box 2By similarity3
Motifi782 – 784KEN box 3By similarity3

Domaini

The KEN box 3 is required for interaction with FZR1/CDH1 and is essential for APC(CDH1)-mediated ubiquitination.By similarity

Sequence similaritiesi

Belongs to the peptidase C19 family.Sequence analysis

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1868 Eukaryota
ENOG410XP8T LUCA
GeneTreeiENSGT00440000033542
HOGENOMiHOG000060197
HOVERGENiHBG055893
InParanoidiQ8C0R0
KOiK11850
PhylomeDBiQ8C0R0
TreeFamiTF323032

Family and domain databases

CDDicd13312 PH_USP37_like, 1 hit
Gene3Di2.30.29.180, 1 hit
InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR003903 UIM_dom
IPR032069 USP37-like_PH
IPR038093 USP37-like_PH_sf
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PF16674 UCH_N, 1 hit
PF02809 UIM, 3 hits
SMARTiView protein in SMART
SM00726 UIM, 3 hits
PROSITEiView protein in PROSITE
PS50330 UIM, 2 hits
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q8C0R0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPLKIYGPI RIRSMQTGIT KWKEGSFEIV EKDNRVSLLV HYNTGGIPRV
60 70 80 90 100
FQLSHNIKNV VLRPSGIKQS RLMLTLQDNS FLSIDKVPSK DAEEMRLFLD
110 120 130 140 150
AVHQNRLHAA MKASQGSGSF GTILGSRTSQ KETNRQLSYS DNQASSKRGS
160 170 180 190 200
LETKDEIPFR KVLGSPGRGP IKTVTGGGMA VTRTIPSLTL TSTPLRSGLL
210 220 230 240 250
ENRTEKRKRM LSGSELTEDY PKENDSSSNN KAMTDPSRKY LTSCREKQLS
260 270 280 290 300
LKQAEENRTS GLLPLQSSSF YGSRAGSKDY SSGVTNLDRC NVSSQTPSAK
310 320 330 340 350
RSLGFLPQPT PLSVKKLRCN QDYAGWNRPR VPLSSHQQQL QGFSNLGNTC
360 370 380 390 400
YMNAILQSLF SLQSFANDLL KQSIPWKKIP FNALIRRFAN LLIKKDICNS
410 420 430 440 450
ETKKELLKKV KNAISATAER FSGYVQNDAH EFLSQCLDQL KEDMEKLNKT
460 470 480 490 500
WKTEPVLGEE NLPDTSATKV FTCPVITNLE FEVQHSIICK ACGETIPKRE
510 520 530 540 550
QFNDLSIDLP RRKKPLPPRS IQDSLDLFFR AEELEYSCEK CGGKCALVRH
560 570 580 590 600
KFNRLPRVLI LHLKRYSFNV ALSLNNKLGQ QVIIPRFLTL ASHCTESTKP
610 620 630 640 650
PVTLGWSAPV AISRPLRACQ MMNSCITSPS APSKKFTFKS KSSVTSCLDS
660 670 680 690 700
DSEDELKRSV VLSQRLCDLP GNEQYQEDVE KDLKLCRLEP GKAELENSGF
710 720 730 740 750
DRMSEEEVLA AVLEISRREA SPVLSPEDDD KPTSSPDTGF AEDDIPEMPE
760 770 780 790 800
NPDAMEIEKS KTITEPGPAS FTEITKDCDE NKENKTPEGS QGEVDWLQQY
810 820 830 840 850
DVDREREEQE LQQALAQSLQ EQEAWEQKED DDLKRATELS LQEFNNSFLD
860 870 880 890 900
SLGSDEDSGN EDVFDMEYTE AEAEELKRNA ETGALPHSYR LISVVSHIGS
910 920 930 940 950
TSSSGHYISD VYDIKKQAWF TYNDLEVSKI QEAAVQSDRD RSGYIFFYMH
960 970
KEIFDELLET EKTSQALSME VGRAARQAS
Length:979
Mass (Da):110,063
Last modified:March 1, 2003 - v1
Checksum:iFF11DB673C1CA902
GO
Isoform 22 Publications (identifier: Q8C0R0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     612-634: ISRPLRACQMMNSCITSPSAPSK → M

Show »
Length:957
Mass (Da):107,733
Checksum:i1ACD5A844FBCB4D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti622M → V in AAI39092 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052196612 – 634ISRPL…SAPSK → M in isoform 2. 2 PublicationsAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030013 mRNA Translation: BAC26734.1
AK035640 mRNA Translation: BAC29135.1
AK173210 mRNA Translation: BAD32488.1
BC139091 mRNA Translation: AAI39092.1
CCDSiCCDS15050.1 [Q8C0R0-1]
CCDS78616.1 [Q8C0R0-2]
RefSeqiNP_001297591.1, NM_001310662.1 [Q8C0R0-2]
NP_795946.2, NM_176972.4
XP_006496117.1, XM_006496054.3
UniGeneiMm.471601
Mm.66568

Genome annotation databases

EnsembliENSMUST00000044260; ENSMUSP00000035445; ENSMUSG00000033364 [Q8C0R0-2]
GeneIDi319651
KEGGimmu:319651
UCSCiuc007bmc.2 mouse [Q8C0R0-2]
uc007bmd.2 mouse [Q8C0R0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBP37_MOUSE
AccessioniPrimary (citable) accession number: Q8C0R0
Secondary accession number(s): B2RT12, Q69ZF6, Q8BZE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health