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Protein

Rap guanine nucleotide exchange factor 5

Gene

Rapgef5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Rap guanine nucleotide exchange factor 5
Alternative name(s):
Guanine nucleotide exchange factor for Rap1
M-Ras-regulated Rap GEF
Short name:
MR-GEF
Gene namesi
Name:Rapgef5
Synonyms:Gfr, Kiaa0277, Mrgef
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2444365. Rapgef5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 814814Rap guanine nucleotide exchange factor 5PRO_0000068874Add
BLAST

Proteomic databases

EPDiQ8C0Q9.
MaxQBiQ8C0Q9.
PaxDbiQ8C0Q9.
PeptideAtlasiQ8C0Q9.
PRIDEiQ8C0Q9.

PTM databases

iPTMnetiQ8C0Q9.
PhosphoSiteiQ8C0Q9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041992.
CleanExiMM_RAPGEF5.
GenevisibleiQ8C0Q9. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105313.

Structurei

3D structure databases

ProteinModelPortaliQ8C0Q9.
SMRiQ8C0Q9. Positions 33-813.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 11876DEPPROSITE-ProRule annotationAdd
BLAST
Domaini301 – 434134N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini578 – 813236Ras-GEFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DEP domain.PROSITE-ProRule annotation
Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2378. Eukaryota.
ENOG410XPX9. LUCA.
GeneTreeiENSGT00810000125357.
HOGENOMiHOG000230545.
HOVERGENiHBG056985.
InParanoidiQ8C0Q9.
KOiK08019.
OMAiELKSYVH.
OrthoDBiEOG091G0HKG.
PhylomeDBiQ8C0Q9.
TreeFamiTF313184.

Family and domain databases

CDDicd06224. REM. 1 hit.
Gene3Di1.10.10.10. 1 hit.
1.10.840.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50186. DEP. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C0Q9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTGDCQTLS RRISNPYLEH SPSQIYGENS SCAGRALRNI IILQAADLVK
60 70 80 90 100
DRVNLKGFYR RSCVGSELVD WLLEHCPFVQ CRSMAIGVWQ LLLDMGIMSS
110 120 130 140 150
VDQHLYFQDN YVFYQFSSDE CSYLYCEFER EEEWQKGVKL LLELVHLIPA
160 170 180 190 200
RAGICDLSHQ KTEDSEESSD EILARLTSAV QRELAAVIAL KARKSAIEQD
210 220 230 240 250
DENADKHVTV TEANNGPDPQ AGVMCKLQER DDIGRIELVH KLARENCQFL
260 270 280 290 300
QTEKKESEKL EQQDDEVTMV QVKEQGQSVL VLKKVASCGP APTSGSAEND
310 320 330 340 350
ARYVVVSGTP EKILEHLLND LHLAEVQHKE TETLLDDFLL TYTVFMTTDD
360 370 380 390 400
LCQALLRHYS AKKYQGEEEN SDVPCRKRKV LHLVSQWISL YKDWLHEDEH
410 420 430 440 450
SKMFLKTIYR NVLDDVYEYP ILEKELKEFQ KILGVYRRHT VDEYSPQKKN
460 470 480 490 500
KALFHQFSLK ENWLQHRGTV AETEEIFCHV YITEHSYISV KAKVSSTAQE
510 520 530 540 550
ILKVVAEKLQ RAEEDLALVA ITFSGEKHEF QPNDLAISKS LEASGRIYVY
560 570 580 590 600
RKDLADTLNP LAENEESQQR SMRILGMNTW DLALELMSFD WSLFNSIHEQ
610 620 630 640 650
ELIYFTFSRQ GNGENTVNLS LLLQRCNEVQ LWVATEILLC SQLGKRVQLV
660 670 680 690 700
KKFIKIAAHC KAQQNLNSFF AIVMGLNTAS VSRLSQTWEK IPGKFKKLFS
710 720 730 740 750
ELESLTDPSL NHKAYRDAFK KMKPPKIPFM PLLLKDVTFI HEGNKTFLDN
760 770 780 790 800
LVNFEKLHMI ADTVRTLRHC RTNQFGSDVS PKEQQELKSY VNHLYVIDSQ
810
QALFELSHRL EPRA
Note: No experimental confirmation available.
Length:814
Mass (Da):93,725
Last modified:June 20, 2003 - v2
Checksum:i2E30E9956D25F0E2
GO
Isoform 2 (identifier: Q8C0Q9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-202: Missing.
     203-220: NADKHVTVTEANNGPDPQ → MSWDCGFKYLFAFSPTLK

Note: No experimental confirmation available.
Show »
Length:612
Mass (Da):70,913
Checksum:iDBD6552DDFD392B6
GO
Isoform 3 (identifier: Q8C0Q9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-234: Missing.
     235-301: RIELVHKLAR...PTSGSAENDA → MGSSRLRVFD...DDSVDSLLSD

Show »
Length:580
Mass (Da):67,547
Checksum:iBB394A21B3C9267B
GO

Sequence cautioni

The sequence BAC65516 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti410 – 4101R → W in BAC26736 (PubMed:12693553).Curated
Sequence conflicti513 – 5131E → K in AAH46627 (PubMed:15489334).Curated
Sequence conflicti646 – 6461R → L in BAC26723 (PubMed:12693553).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 234234Missing in isoform 3. 2 PublicationsVSP_007619Add
BLAST
Alternative sequencei1 – 202202Missing in isoform 2. 1 PublicationVSP_007617Add
BLAST
Alternative sequencei203 – 22018NADKH…GPDPQ → MSWDCGFKYLFAFSPTLK in isoform 2. 1 PublicationVSP_007618Add
BLAST
Alternative sequencei235 – 30167RIELV…AENDA → MGSSRLRVFDPPLERKDSAA LSERQLPLPTFDVPYFKYID EEDEDDEWSSRSQSSTEDDS VDSLLSD in isoform 3. 2 PublicationsVSP_007620Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030016 mRNA. Translation: BAC26736.1.
AK029995 mRNA. Translation: BAC26723.1.
AK083591 mRNA. Translation: BAC38963.1.
AK122234 mRNA. Translation: BAC65516.1. Different initiation.
BC046627 mRNA. Translation: AAH46627.1.
CCDSiCCDS36577.1. [Q8C0Q9-1]
RefSeqiNP_787126.3. NM_175930.5. [Q8C0Q9-1]
UniGeneiMm.227642.

Genome annotation databases

EnsembliENSMUST00000109691; ENSMUSP00000105313; ENSMUSG00000041992. [Q8C0Q9-1]
GeneIDi217944.
KEGGimmu:217944.
UCSCiuc007pib.2. mouse. [Q8C0Q9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030016 mRNA. Translation: BAC26736.1.
AK029995 mRNA. Translation: BAC26723.1.
AK083591 mRNA. Translation: BAC38963.1.
AK122234 mRNA. Translation: BAC65516.1. Different initiation.
BC046627 mRNA. Translation: AAH46627.1.
CCDSiCCDS36577.1. [Q8C0Q9-1]
RefSeqiNP_787126.3. NM_175930.5. [Q8C0Q9-1]
UniGeneiMm.227642.

3D structure databases

ProteinModelPortaliQ8C0Q9.
SMRiQ8C0Q9. Positions 33-813.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105313.

PTM databases

iPTMnetiQ8C0Q9.
PhosphoSiteiQ8C0Q9.

Proteomic databases

EPDiQ8C0Q9.
MaxQBiQ8C0Q9.
PaxDbiQ8C0Q9.
PeptideAtlasiQ8C0Q9.
PRIDEiQ8C0Q9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109691; ENSMUSP00000105313; ENSMUSG00000041992. [Q8C0Q9-1]
GeneIDi217944.
KEGGimmu:217944.
UCSCiuc007pib.2. mouse. [Q8C0Q9-1]

Organism-specific databases

CTDi9771.
MGIiMGI:2444365. Rapgef5.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2378. Eukaryota.
ENOG410XPX9. LUCA.
GeneTreeiENSGT00810000125357.
HOGENOMiHOG000230545.
HOVERGENiHBG056985.
InParanoidiQ8C0Q9.
KOiK08019.
OMAiELKSYVH.
OrthoDBiEOG091G0HKG.
PhylomeDBiQ8C0Q9.
TreeFamiTF313184.

Miscellaneous databases

ChiTaRSiRapgef5. mouse.
PROiQ8C0Q9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041992.
CleanExiMM_RAPGEF5.
GenevisibleiQ8C0Q9. MM.

Family and domain databases

CDDicd06224. REM. 1 hit.
Gene3Di1.10.10.10. 1 hit.
1.10.840.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR029071. Ubiquitin-rel_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00610. DEP. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48366. SSF48366. 2 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50186. DEP. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPGF5_MOUSE
AccessioniPrimary (citable) accession number: Q8C0Q9
Secondary accession number(s): Q8BJJ9, Q8C0R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.