Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase Nek11

Gene

Nek11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mn2+By similarity, Mg2+By similarity

Enzyme regulationi

Autorepressed by intramolecular binding of the C-terminus which dissociates following phosphorylation by NEK2. Activated in response to DNA damage. Inhibited by zinc (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621ATPPROSITE-ProRule annotationBy similarity
Active sitei159 – 1591Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 449ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek11 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 11
Short name:
NimA-related protein kinase 11
Gene namesi
Name:Nek11Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2442276. Nek11.

Subcellular locationi

  • Nucleus By similarity
  • Nucleusnucleolus By similarity

  • Note: Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628Serine/threonine-protein kinase Nek11PRO_0000086440Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei274 – 2741Phosphoserine; by CHEK1By similarity

Post-translational modificationi

Phosphorylated by NEK2. Phosphorylation at Ser-274 is important for its activation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8C0Q4.
PRIDEiQ8C0Q4.

PTM databases

iPTMnetiQ8C0Q4.
PhosphoSiteiQ8C0Q4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035032.
ExpressionAtlasiQ8C0Q4. baseline and differential.

Interactioni

Subunit structurei

Interacts with NEK2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038611.

Structurei

3D structure databases

ProteinModelPortaliQ8C0Q4.
SMRiQ8C0Q4. Positions 15-360.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 288259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili347 – 38539Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
ENOG410Y7JF. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000293260.
HOVERGENiHBG082016.
InParanoidiQ8C0Q4.
KOiK08857.
OMAiIPEDPQI.
OrthoDBiEOG091G0FBH.
TreeFamiTF106472.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C0Q4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFQETAKC VGRRPTVIPM YPTALIARRY VLQQKLGSGS FGTVYLVSDK
60 70 80 90 100
KAKPGEELKV LKEISVGELN PNETVQANVE AQLLSRLHHP AIVRFHASFM
110 120 130 140 150
EQETFCIITE YCEGRDLDYR IQEYKEAGKV FAENQIVEWF IQLLLGVDYM
160 170 180 190 200
HERRILHRDL KSKNIFLKNN LLKIGDFGVS RLLMGSCELA TTLTGTPHYM
210 220 230 240 250
SPEALKHQGY DAKSDIWSLA CILYEMCCLD HAFAGSSFLS VVLNIVEGKT
260 270 280 290 300
PSLPDRYPRE LNTIMERMLN KSPSLRPSAA DILKAPYMEE QLQLLMCKYP
310 320 330 340 350
EMTLEDKNSV CQKEAAHTIN AVQKKLHLQT LQALSDTQKT TPRERMWLRK
360 370 380 390 400
LQAADERARR LKKIAEENYK ENDKRMQALR SRNVGSVHAH VLHELDERTL
410 420 430 440 450
ESLPEPQSLP CLDLDELEPS LEDTIVDLGH YEIPEDPLVA EQYYSDVFDS
460 470 480 490 500
CSEDSEEQEE EMIFSEAGGD TKEEESPSVY RTNQQDSDTA ALVGCLEHVL
510 520 530 540 550
GYTSLDTKTI TNAVTDMSPG PMVFNSAVAR TKMKRMKESA VQKLGMETFE
560 570 580 590 600
EVYDYLKRAR HQNAREAEIW EHLETVVPRA SDCFEVDQLL YFEELLLTME
610 620
GKEPSLQNLP CEAAQKKPVK GTHFCDNP
Length:628
Mass (Da):71,648
Last modified:July 27, 2011 - v2
Checksum:i2D8672AA753CEB84
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti196 – 1961T → A in BAC31576 (PubMed:16141072).Curated
Sequence conflicti433 – 4386IPEDPL → KRKKER in BAC31576 (PubMed:16141072).Curated
Sequence conflicti561 – 5611H → R in BAC26756 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030042 mRNA. Translation: BAC26756.1.
AK043543 mRNA. Translation: BAC31576.1.
AK054237 mRNA. Translation: BAC35699.1.
AC113016 Genomic DNA. No translation available.
AC161250 Genomic DNA. No translation available.
CCDSiCCDS23464.1.
RefSeqiNP_766049.2. NM_172461.3.
XP_006511742.1. XM_006511679.2.
UniGeneiMm.347509.

Genome annotation databases

EnsembliENSMUST00000038648; ENSMUSP00000038611; ENSMUSG00000035032.
GeneIDi208583.
KEGGimmu:208583.
UCSCiuc009rhy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030042 mRNA. Translation: BAC26756.1.
AK043543 mRNA. Translation: BAC31576.1.
AK054237 mRNA. Translation: BAC35699.1.
AC113016 Genomic DNA. No translation available.
AC161250 Genomic DNA. No translation available.
CCDSiCCDS23464.1.
RefSeqiNP_766049.2. NM_172461.3.
XP_006511742.1. XM_006511679.2.
UniGeneiMm.347509.

3D structure databases

ProteinModelPortaliQ8C0Q4.
SMRiQ8C0Q4. Positions 15-360.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038611.

PTM databases

iPTMnetiQ8C0Q4.
PhosphoSiteiQ8C0Q4.

Proteomic databases

PaxDbiQ8C0Q4.
PRIDEiQ8C0Q4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038648; ENSMUSP00000038611; ENSMUSG00000035032.
GeneIDi208583.
KEGGimmu:208583.
UCSCiuc009rhy.2. mouse.

Organism-specific databases

CTDi79858.
MGIiMGI:2442276. Nek11.

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
ENOG410Y7JF. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000293260.
HOVERGENiHBG082016.
InParanoidiQ8C0Q4.
KOiK08857.
OMAiIPEDPQI.
OrthoDBiEOG091G0FBH.
TreeFamiTF106472.

Miscellaneous databases

PROiQ8C0Q4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035032.
ExpressionAtlasiQ8C0Q4. baseline and differential.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEK11_MOUSE
AccessioniPrimary (citable) accession number: Q8C0Q4
Secondary accession number(s): E9QNC3, Q8BLS6, Q8BW62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.