Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8C0Q2

- ZHX3_MOUSE

UniProt

Q8C0Q2 - ZHX3_MOUSE

Protein

Zinc fingers and homeoboxes protein 3

Gene

Zhx3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (16 Feb 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts as a transcriptional repressor. Involved in the early stages of mesenchymal stem cell (MSC) osteogenic differentiation. Is a regulator of podocyte gene expression during primary glomerula disease. Binds to promoter DNA.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri77 – 10024C2H2-type 1Sequence AnalysisAdd
    BLAST
    Zinc fingeri109 – 13224C2H2-type 2Sequence AnalysisAdd
    BLAST
    DNA bindingi300 – 35960Homeobox 1PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi489 – 54860Homeobox 2PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi607 – 66660Homeobox 3PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi759 – 81860Homeobox 4PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi830 – 88960Homeobox 5PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: MGI
    3. protein heterodimerization activity Source: UniProtKB
    4. protein homodimerization activity Source: UniProtKB
    5. sequence-specific DNA binding Source: InterPro
    6. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    7. transcription corepressor activity Source: Ensembl

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. negative regulation of transcription, DNA-templated Source: UniProtKB
    3. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    4. positive regulation of osteoblast differentiation Source: UniProtKB
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Differentiation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc fingers and homeoboxes protein 3
    Alternative name(s):
    Triple homeobox protein 1
    Zinc finger and homeodomain protein 3
    Gene namesi
    Name:Zhx3
    Synonyms:Kiaa0395, Tix1Imported
    OrganismiMus musculus (Mouse)Imported
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2444772. Zhx3.

    Subcellular locationi

    Nucleus By similarityPROSITE-ProRule annotation

    GO - Cellular componenti

    1. cell cortex Source: MGI
    2. nucleolus Source: Ensembl
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 951951Zinc fingers and homeoboxes protein 3PRO_0000049396Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei703 – 7031Phosphoserine1 Publication
    Modified residuei941 – 9411PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8C0Q2.
    PaxDbiQ8C0Q2.
    PRIDEiQ8C0Q2.

    PTM databases

    PhosphoSiteiQ8C0Q2.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ8C0Q2.
    BgeeiQ8C0Q2.
    CleanExiMM_ZHX3.
    GenevestigatoriQ8C0Q2.

    Interactioni

    Subunit structurei

    Homodimer (via homeobox domain). Also forms heterodimers with ZHX1 and ZHX2. Heterodimerization with ZHX1 is a prerequisite for repressor activity. Interacts with NFYA.

    Protein-protein interaction databases

    IntActiQ8C0Q2. 1 interaction.
    MINTiMINT-4140601.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8C0Q2.
    SMRiQ8C0Q2. Positions 77-157, 311-355, 491-552, 608-670, 765-829.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 107107Required for nuclear localizationBy similarityAdd
    BLAST
    Regioni238 – 483246Required for homodimerization and interaction with NFYABy similarityAdd
    BLAST
    Regioni299 – 497199Required for repressor activityBy similarityAdd
    BLAST
    Regioni492 – 55059Required for nuclear localizationBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ZHX family.Sequence Analysis
    Contains 2 C2H2-type zinc fingers.Sequence Analysis
    Contains 5 homeobox DNA-binding domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri77 – 10024C2H2-type 1Sequence AnalysisAdd
    BLAST
    Zinc fingeri109 – 13224C2H2-type 2Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Homeobox, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG39488.
    GeneTreeiENSGT00530000063149.
    HOGENOMiHOG000013231.
    HOVERGENiHBG007920.
    OMAiMKGKAEA.
    OrthoDBiEOG76HQ0T.
    PhylomeDBiQ8C0Q2.
    TreeFamiTF333363.

    Family and domain databases

    Gene3Di1.10.10.60. 5 hits.
    InterProiIPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 3 hits.
    [Graphical view]
    SMARTiSM00389. HOX. 4 hits.
    SM00355. ZnF_C2H2. 2 hits.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 5 hits.
    PROSITEiPS50071. HOMEOBOX_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8C0Q2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASKRKSTTP CMIPVKTVVL PGASTEPQPV ESLPEGPQQD LPSEAPDASS    50
    EAAPNPSSTD GSALANGHRS TLDGYVYCCK ECEFRSQDVT HFIGHMNSEH 100
    TDFNKDPTFV CTGCSFLAKN PEGLSLHNAK CHSGEASFLW NVTKPDNHVV 150
    VEQSVPDSAS SSVLAGESTT EGTEIIITKT PIMKIMKGKA EAKKIHMLKE 200
    NAPNQPGSEA LPKPLAGERE VKEGDHTFIN GAAPGSQASA KSTKPPPAAN 250
    GPLIGTVPVL PAGIAQFLSL QQQPPVHAQH HTHQPLPTSK TLPKVMIPLS 300
    SIPTYNAAMD SNSFLKNSFH KFPYPTKAEL CYLTVVTKYP EEQLKIWFTA 350
    QRLKQGISWS PEEIEDARKK MFNTVIQSVP QPTITVLNTP LVASAGNVQH 400
    LIQATLPGHA VGQPEGTAGG LLVTQPLMAN GLQASSSSLP LTTASVPKPT 450
    VAPINTVCSN SASAVKVVNA AQSLLTACPS ITSQAFLDAN IYKNKKSHEQ 500
    LSALKGSFCR NQFPGQSEVE HLTKVTGLST REVRKWFSDR RYHCRNLKGS 550
    RAMMPGEHGS VLIDSVPEVP FPLASKVPEV TCIPTATSLV SHPATKRQSW 600
    HQTPDFTPTK YKERAPEQLR VLENSFAQNP LPPEEELDRL RSETKMTRRE 650
    IDGWFSERRK KVNTEETKKA DGHMPKEEEE GAEQEGRDEE LANELRVPGE 700
    NGSPEMFLSH ALAERKVSPI KINLKNLRVT EASGKSEFPG MGVCEPEEDG 750
    LNKLVEQPPS KVSYKKTAQQ RHLLRQLFVQ TQWPSNQDYD SIMAQTGLPR 800
    PEVVRWFGDS RYALKNGQLK WYEDYKRGNF PPGLLVIAPG NRELLQDYYM 850
    THKMLCEEDL QTLCDKTQMS AQQVKQWFAE KMGEETRAVA DISSEDQGPR 900
    NGEPVAVHKV LGDAYSELSE NSESWEPSAP EASSEPFDTS SPQSGRQLEA 950
    D 951
    Length:951
    Mass (Da):104,343
    Last modified:February 16, 2004 - v2
    Checksum:iC6625D6FB139E3DE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti763 – 7631S → N in BAC26763. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB099527 mRNA. Translation: BAC87711.2.
    AB099703 Genomic DNA. Translation: BAC87741.2.
    AK030057 mRNA. Translation: BAC26763.1.
    BC058111 mRNA. Translation: AAH58111.1.
    AK122271 mRNA. Translation: BAC65553.1.
    CCDSiCCDS16997.1.
    RefSeqiNP_796237.2. NM_177263.3.
    UniGeneiMm.436734.
    Mm.489808.

    Genome annotation databases

    EnsembliENSMUST00000103111; ENSMUSP00000099400; ENSMUSG00000035877.
    ENSMUST00000103112; ENSMUSP00000099401; ENSMUSG00000035877.
    ENSMUST00000109460; ENSMUSP00000105086; ENSMUSG00000035877.
    GeneIDi320799.
    KEGGimmu:320799.
    UCSCiuc008nre.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB099527 mRNA. Translation: BAC87711.2 .
    AB099703 Genomic DNA. Translation: BAC87741.2 .
    AK030057 mRNA. Translation: BAC26763.1 .
    BC058111 mRNA. Translation: AAH58111.1 .
    AK122271 mRNA. Translation: BAC65553.1 .
    CCDSi CCDS16997.1.
    RefSeqi NP_796237.2. NM_177263.3.
    UniGenei Mm.436734.
    Mm.489808.

    3D structure databases

    ProteinModelPortali Q8C0Q2.
    SMRi Q8C0Q2. Positions 77-157, 311-355, 491-552, 608-670, 765-829.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8C0Q2. 1 interaction.
    MINTi MINT-4140601.

    PTM databases

    PhosphoSitei Q8C0Q2.

    Proteomic databases

    MaxQBi Q8C0Q2.
    PaxDbi Q8C0Q2.
    PRIDEi Q8C0Q2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103111 ; ENSMUSP00000099400 ; ENSMUSG00000035877 .
    ENSMUST00000103112 ; ENSMUSP00000099401 ; ENSMUSG00000035877 .
    ENSMUST00000109460 ; ENSMUSP00000105086 ; ENSMUSG00000035877 .
    GeneIDi 320799.
    KEGGi mmu:320799.
    UCSCi uc008nre.1. mouse.

    Organism-specific databases

    CTDi 23051.
    MGIi MGI:2444772. Zhx3.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG39488.
    GeneTreei ENSGT00530000063149.
    HOGENOMi HOG000013231.
    HOVERGENi HBG007920.
    OMAi MKGKAEA.
    OrthoDBi EOG76HQ0T.
    PhylomeDBi Q8C0Q2.
    TreeFami TF333363.

    Miscellaneous databases

    NextBioi 397455.
    PROi Q8C0Q2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8C0Q2.
    Bgeei Q8C0Q2.
    CleanExi MM_ZHX3.
    Genevestigatori Q8C0Q2.

    Family and domain databases

    Gene3Di 1.10.10.60. 5 hits.
    InterProi IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 3 hits.
    [Graphical view ]
    SMARTi SM00389. HOX. 4 hits.
    SM00355. ZnF_C2H2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 5 hits.
    PROSITEi PS50071. HOMEOBOX_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mouse zinc-fingers and homeoboxes (ZHX) family: ZHX2 forms a heterodimer with ZHX3."
      Kawata H., Yamada K., Shou Z., Mizutani T., Miyamoto K.
      Gene 323:133-140(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], HETERODIMERIZATION WITH ZHX2, TISSUE SPECIFICITY.
      Strain: 1291 Publication.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported.
      Tissue: TestisImported.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6Imported.
      Tissue: BrainImported.
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
      DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 754-951.
      Tissue: BrainImported.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-703, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiZHX3_MOUSE
    AccessioniPrimary (citable) accession number: Q8C0Q2
    Secondary accession number(s): Q80U14
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: February 16, 2004
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3