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Q8C0N2

- GPAT3_MOUSE

UniProt

Q8C0N2 - GPAT3_MOUSE

Protein

Glycerol-3-phosphate acyltransferase 3

Gene

Agpat9

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    May transfer the acyl-group from acyl-coA to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Also transfers the acyl-group from acyl-coA to the sn-2 position of 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid, or LPA), forming 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid, or PA).1 Publication

    Catalytic activityi

    Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
    Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. 1-acylglycerol-3-phosphate O-acyltransferase activity Source: UniProtKB-EC
    2. glycerol-3-phosphate O-acyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
    2. regulation of TOR signaling Source: Ensembl
    3. triglyceride biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_188640. Synthesis of PA.
    UniPathwayiUPA00282.
    UPA00557; UER00612.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate acyltransferase 3 (EC:2.3.1.15)
    Short name:
    GPAT-3
    Alternative name(s):
    1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9 (EC:2.3.1.51)
    Short name:
    1-AGP acyltransferase 9
    Short name:
    1-AGPAT 9
    Acyl-CoA:glycerol-3-phosphate acyltransferase 3
    Short name:
    mGPAT3
    Lysophosphatidic acid acyltransferase theta
    Short name:
    LPAAT-theta
    Gene namesi
    Name:Agpat9
    Synonyms:Gpat3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:3603816. Agpat9.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 438438Glycerol-3-phosphate acyltransferase 3PRO_0000291571Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei68 – 681Phosphoserine3 Publications
    Modified residuei77 – 771PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8C0N2.
    PaxDbiQ8C0N2.
    PRIDEiQ8C0N2.

    PTM databases

    PhosphoSiteiQ8C0N2.

    Expressioni

    Tissue specificityi

    Most abundant in epididymal fat, followed by small intestine, brown adipose tissue, kidney, heart and colon.1 Publication

    Inductioni

    During adipocyte differentiation.1 Publication

    Gene expression databases

    BgeeiQ8C0N2.
    CleanExiMM_AGPAT9.
    GenevestigatoriQ8C0N2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8C0N2.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei14 – 3421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei137 – 15721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei161 – 18121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi229 – 2346HXXXXD motif

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0204.
    GeneTreeiENSGT00390000000536.
    HOGENOMiHOG000265725.
    InParanoidiA6H5X0.
    KOiK13506.
    OMAiLVRYCVL.
    PhylomeDBiQ8C0N2.
    TreeFamiTF315039.

    Family and domain databases

    InterProiIPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view]
    PfamiPF01553. Acyltransferase. 1 hit.
    [Graphical view]
    SMARTiSM00563. PlsC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8C0N2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGADLAVKL LSTWLTLVGG LILLPSAFGL SLGISEIYMK ILVKTLEWAT    50
    LRIQKGAPKE SALKNSASVG IIQRDESPME KGLSGLRGRD FELSDVFYFS 100
    KKGLEAIVED EVTQRFSSEE LVSWNLLTRT NVNFQYISPR LTMVWVLGVL 150
    VRYCFLLPLR VTLAFIGISL LIIGTTLVGQ LPDSSLKNWL SELVHLTCCR 200
    ICVRSLSGTI HYHNKQYRPQ KGGICVANHT SPIDVLILAT DGCYAMVGQV 250
    HGGLMGIIQR AMVKACPHVW FERSEIKDRH LVTKRLKEHI ADKKKLPILI 300
    FPEGTCINNT SVMMFKKGSF EIGGTIYPVA IKYNPQFGDA FWNSSKYNLV 350
    SYLLRIMTSW AIVCDVWYMP PMTREEGEDA VQFANRVKSA IAVQGGLTEL 400
    PWDGGLKRAK VKDTFKEEQQ KNYSKMIVGN GSPNLARD 438
    Length:438
    Mass (Da):49,000
    Last modified:March 1, 2003 - v1
    Checksum:i29B4ED7405DCC8BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030171 mRNA. Translation: BAC26820.1.
    AK160261 mRNA. Translation: BAE35719.1.
    AK138410 mRNA. Translation: BAE23647.1.
    BC096769 mRNA. Translation: AAH96769.1.
    BC138228 mRNA. Translation: AAI38229.1.
    BC145669 mRNA. Translation: AAI45670.1.
    CCDSiCCDS19470.1.
    RefSeqiNP_766303.1. NM_172715.3.
    UniGeneiMm.271911.

    Genome annotation databases

    EnsembliENSMUST00000031255; ENSMUSP00000031255; ENSMUSG00000029314.
    ENSMUST00000092990; ENSMUSP00000090667; ENSMUSG00000029314.
    ENSMUST00000112887; ENSMUSP00000108508; ENSMUSG00000029314.
    GeneIDi231510.
    KEGGimmu:231510.
    UCSCiuc008yih.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK030171 mRNA. Translation: BAC26820.1 .
    AK160261 mRNA. Translation: BAE35719.1 .
    AK138410 mRNA. Translation: BAE23647.1 .
    BC096769 mRNA. Translation: AAH96769.1 .
    BC138228 mRNA. Translation: AAI38229.1 .
    BC145669 mRNA. Translation: AAI45670.1 .
    CCDSi CCDS19470.1.
    RefSeqi NP_766303.1. NM_172715.3.
    UniGenei Mm.271911.

    3D structure databases

    ProteinModelPortali Q8C0N2.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8C0N2.

    Proteomic databases

    MaxQBi Q8C0N2.
    PaxDbi Q8C0N2.
    PRIDEi Q8C0N2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031255 ; ENSMUSP00000031255 ; ENSMUSG00000029314 .
    ENSMUST00000092990 ; ENSMUSP00000090667 ; ENSMUSG00000029314 .
    ENSMUST00000112887 ; ENSMUSP00000108508 ; ENSMUSG00000029314 .
    GeneIDi 231510.
    KEGGi mmu:231510.
    UCSCi uc008yih.1. mouse.

    Organism-specific databases

    CTDi 84803.
    MGIi MGI:3603816. Agpat9.

    Phylogenomic databases

    eggNOGi COG0204.
    GeneTreei ENSGT00390000000536.
    HOGENOMi HOG000265725.
    InParanoidi A6H5X0.
    KOi K13506.
    OMAi LVRYCVL.
    PhylomeDBi Q8C0N2.
    TreeFami TF315039.

    Enzyme and pathway databases

    UniPathwayi UPA00282 .
    UPA00557 ; UER00612 .
    Reactomei REACT_188640. Synthesis of PA.

    Miscellaneous databases

    NextBioi 380592.
    PROi Q8C0N2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8C0N2.
    CleanExi MM_AGPAT9.
    Genevestigatori Q8C0N2.

    Family and domain databases

    InterProi IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view ]
    Pfami PF01553. Acyltransferase. 1 hit.
    [Graphical view ]
    SMARTi SM00563. PlsC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Hypothalamus and Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CD-1.
      Tissue: Brain, Neural stem cell and Testis.
    3. "Molecular identification of microsomal acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis."
      Cao J., Li J.-L., Li D., Tobin J.F., Gimeno R.E.
      Proc. Natl. Acad. Sci. U.S.A. 103:19695-19700(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGPAT3_MOUSE
    AccessioniPrimary (citable) accession number: Q8C0N2
    Secondary accession number(s): A6H5X0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Despite its name, the human ortholog of this protein appears to lack measurable glycerol-3-phosphate acyltransferase activity under some conditions (PMID:19318427).Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3