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Q8C0J2

- A16L1_MOUSE

UniProt

Q8C0J2 - A16L1_MOUSE

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Protein
Autophagy-related protein 16-1
Gene
Atg16l1, Apg16l
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.2 Publications

GO - Molecular functioni

  1. protein binding Source: UniProtKB

GO - Biological processi

  1. autophagic vacuole assembly Source: MGI
  2. negative stranded viral RNA replication Source: MGI
  3. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 16-1
Alternative name(s):
APG16-like 1
Gene namesi
Name:Atg16l1
Synonyms:Apg16l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1924290. Atg16l1.

Subcellular locationi

Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein
Note: Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.2 Publications

GO - Cellular componenti

  1. autophagic vacuole Source: MGI
  2. autophagic vacuole membrane Source: MGI
  3. axoneme Source: UniProtKB
  4. pre-autophagosomal structure membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi299 – 2991D → E: Prevents cleavage by activated CASP3. 1 Publication
Mutagenesisi300 – 3001T → A: No effect on the stability of the protein. Enhances cleavage and degradation mediated by activated CASP3. Results in reduced autophagy and defective clearance of intestinal pathogens. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Autophagy-related protein 16-1
PRO_0000050849Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei269 – 2691Phosphoserine By similarity
Modified residuei287 – 2871Phosphoserine By similarity

Post-translational modificationi

Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8C0J2.
PaxDbiQ8C0J2.
PRIDEiQ8C0J2.

2D gel databases

REPRODUCTION-2DPAGEQ8C0J2.

PTM databases

PhosphoSiteiQ8C0J2.

Expressioni

Tissue specificityi

Widely expressed. In the liver, isoform 2 is highly expressed and isoform 1 is weakly expressed. Isoform 3 is expressed in the brain.1 Publication

Gene expression databases

ArrayExpressiQ8C0J2.
BgeeiQ8C0J2.
CleanExiMM_ATG16L1.
GenevestigatoriQ8C0J2.

Interactioni

Subunit structurei

Interacts with TMEM59, TLR2 and NOD2 By similarity. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rb1cc1Q9ESK93EBI-769195,EBI-647302

Protein-protein interaction databases

BioGridi218476. 5 interactions.
DIPiDIP-31966N.
IntActiQ8C0J2. 4 interactions.
MINTiMINT-136268.

Structurei

3D structure databases

ProteinModelPortaliQ8C0J2.
SMRiQ8C0J2. Positions 267-604.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati320 – 35940WD 1
Add
BLAST
Repeati364 – 40340WD 2
Add
BLAST
Repeati406 – 44540WD 3
Add
BLAST
Repeati447 – 48438WD 4
Add
BLAST
Repeati486 – 52540WD 5
Add
BLAST
Repeati532 – 57342WD 6
Add
BLAST
Repeati575 – 60733WD 7
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili79 – 230152 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi296 – 2994Caspase cleavage

Sequence similaritiesi

Belongs to the WD repeat ATG16 family.
Contains 7 WD repeats.

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00740000115355.
HOVERGENiHBG050534.
KOiK17890.
OMAiLETECQE.
OrthoDBiEOG70CR6M.
PhylomeDBiQ8C0J2.
TreeFamiTF315541.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR013923. Autophagy-rel_prot_16.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08614. ATG16. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8C0J2-1) [UniParc]FASTAAdd to Basket

Also known as: Apg16Lbeta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSGLRAADF PRWKRHIAEE LRRRDRLQRQ AFEEIILQYT KLLEKSDLHS    50
VLTQKLQAEK HDMPNRHEIS PGHDGAWNDS QLQEMAQLRI KHQEELTELH 100
KKRGELAQLV IDLNNQMQQK DKEIQMNEAK ISEYLQTISD LETNCLDLRT 150
KLQDLEVANQ TLKDEYDALQ ITFTALEEKL RKTTEENQEL VTRWMAEKAQ 200
EANRLNAENE KDSRRRQARL QKELAEAAKE PLPVEQDDDI EVIVDETSDH 250
TEETSPVRAV SRAATKRLSQ PAGGLLDSIT NIFGRRSVSS IPVPQDIMDT 300
HPASGKDVRV PTTASYVFDA HDGEVNAVQF SPGSRLLATG GMDRRVKLWE 350
AFGDKCEFKG SLSGSNAGIT SIEFDSAGAY LLAASNDFAS RIWTVDDYRL 400
RHTLTGHSGK VLSAKFLLDN ARIVSGSHDR TLKLWDLRSK VCIKTVFAGS 450
SCNDIVCTEQ CVMSGHFDKK IRFWDIRSES VVREMELLGK ITALDLNPER 500
TELLSCSRDD LLKVIDLRTN AVKQTFSAPG FKCGSDWTRV VFSPDGSYVA 550
AGSAEGSLYV WSVLTGKVEK VLSKQHSSSI NAVAWAPSGL HVVSVDKGSR 600
AVLWAQP 607
Length:607
Mass (Da):68,172
Last modified:March 1, 2003 - v1
Checksum:i63920320883A69DF
GO
Isoform 2 (identifier: Q8C0J2-2) [UniParc]FASTAAdd to Basket

Also known as: Apg16Lalpha

The sequence of this isoform differs from the canonical sequence as follows:
     266-284: Missing.

Note: No experimental confirmation available.

Show »
Length:588
Mass (Da):66,203
Checksum:iCE2AB829A7911274
GO
Isoform 3 (identifier: Q8C0J2-3) [UniParc]FASTAAdd to Basket

Also known as: Apg16Lgamma

The sequence of this isoform differs from the canonical sequence as follows:
     284-284: G → GLSESPLLGHHSSDAAR

Show »
Length:623
Mass (Da):69,831
Checksum:i823453BB5D7DE6FE
GO
Isoform 4 (identifier: Q8C0J2-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-284: G → GLSESPLLGHHSSDAAR
     443-452: IKTVFAGSSC → EEMQSLCVFM
     453-607: Missing.

Note: No experimental confirmation available.

Show »
Length:468
Mass (Da):53,024
Checksum:iFFB7AC4AE34DF8F7
GO
Isoform 5 (identifier: Q8C0J2-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     266-284: Missing.
     442-442: C → CEEMQSLCVLMVFGFLSG

Note: No experimental confirmation available.

Show »
Length:605
Mass (Da):68,075
Checksum:iB0BB8DE0748401B6
GO

Sequence cautioni

The sequence BAD21370.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei266 – 28419Missing in isoform 2 and isoform 5.
VSP_013391Add
BLAST
Alternative sequencei284 – 2841G → GLSESPLLGHHSSDAAR in isoform 3 and isoform 4.
VSP_013392
Alternative sequencei442 – 4421C → CEEMQSLCVLMVFGFLSG in isoform 5.
VSP_013393
Alternative sequencei443 – 45210IKTVFAGSSC → EEMQSLCVFM in isoform 4.
VSP_013394
Alternative sequencei453 – 607155Missing in isoform 4.
VSP_013395Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831Q → H in BAC55090. 1 Publication
Sequence conflicti83 – 831Q → H in BAC55091. 1 Publication
Sequence conflicti83 – 831Q → H in BAC55092. 1 Publication
Sequence conflicti83 – 831Q → H in BAB23866. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB087879 mRNA. Translation: BAC55090.1.
AB087880 mRNA. Translation: BAC55091.1.
AB087881 mRNA. Translation: BAC55092.1.
AK005181 mRNA. Translation: BAB23866.1.
AK030983 mRNA. Translation: BAC27201.1.
AK131120 Transcribed RNA. Translation: BAD21370.1. Different initiation.
BC049122 mRNA. Translation: AAH49122.1.
CCDSiCCDS15136.1. [Q8C0J2-1]
CCDS56637.1. [Q8C0J2-3]
CCDS56638.1. [Q8C0J2-2]
RefSeqiNP_001192320.1. NM_001205391.1. [Q8C0J2-3]
NP_084122.2. NM_029846.4. [Q8C0J2-1]
UniGeneiMm.272972.
Mm.456099.

Genome annotation databases

EnsembliENSMUST00000027512; ENSMUSP00000027512; ENSMUSG00000026289. [Q8C0J2-1]
ENSMUST00000113186; ENSMUSP00000108811; ENSMUSG00000026289. [Q8C0J2-2]
ENSMUST00000113190; ENSMUSP00000108815; ENSMUSG00000026289. [Q8C0J2-3]
GeneIDi77040.
KEGGimmu:77040.
UCSCiuc007bxl.2. mouse. [Q8C0J2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB087879 mRNA. Translation: BAC55090.1 .
AB087880 mRNA. Translation: BAC55091.1 .
AB087881 mRNA. Translation: BAC55092.1 .
AK005181 mRNA. Translation: BAB23866.1 .
AK030983 mRNA. Translation: BAC27201.1 .
AK131120 Transcribed RNA. Translation: BAD21370.1 . Different initiation.
BC049122 mRNA. Translation: AAH49122.1 .
CCDSi CCDS15136.1. [Q8C0J2-1 ]
CCDS56637.1. [Q8C0J2-3 ]
CCDS56638.1. [Q8C0J2-2 ]
RefSeqi NP_001192320.1. NM_001205391.1. [Q8C0J2-3 ]
NP_084122.2. NM_029846.4. [Q8C0J2-1 ]
UniGenei Mm.272972.
Mm.456099.

3D structure databases

ProteinModelPortali Q8C0J2.
SMRi Q8C0J2. Positions 267-604.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 218476. 5 interactions.
DIPi DIP-31966N.
IntActi Q8C0J2. 4 interactions.
MINTi MINT-136268.

PTM databases

PhosphoSitei Q8C0J2.

2D gel databases

REPRODUCTION-2DPAGE Q8C0J2.

Proteomic databases

MaxQBi Q8C0J2.
PaxDbi Q8C0J2.
PRIDEi Q8C0J2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027512 ; ENSMUSP00000027512 ; ENSMUSG00000026289 . [Q8C0J2-1 ]
ENSMUST00000113186 ; ENSMUSP00000108811 ; ENSMUSG00000026289 . [Q8C0J2-2 ]
ENSMUST00000113190 ; ENSMUSP00000108815 ; ENSMUSG00000026289 . [Q8C0J2-3 ]
GeneIDi 77040.
KEGGi mmu:77040.
UCSCi uc007bxl.2. mouse. [Q8C0J2-1 ]

Organism-specific databases

CTDi 55054.
MGIi MGI:1924290. Atg16l1.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00740000115355.
HOVERGENi HBG050534.
KOi K17890.
OMAi LETECQE.
OrthoDBi EOG70CR6M.
PhylomeDBi Q8C0J2.
TreeFami TF315541.

Miscellaneous databases

NextBioi 346354.
PROi Q8C0J2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8C0J2.
Bgeei Q8C0J2.
CleanExi MM_ATG16L1.
Genevestigatori Q8C0J2.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR013923. Autophagy-rel_prot_16.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF08614. ATG16. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view ]
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate."
    Mizushima N., Kuma A., Kobayashi Y., Yamamoto A., Matsubae M., Takao T., Natsume T., Ohsumi Y., Yoshimori T.
    J. Cell Sci. 116:1679-1688(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH ATG5.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
    Strain: C57BL/6J.
    Tissue: Cerebellum and Thymus.
  3. "Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.
    DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
    Tissue: Fetal brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation."
    Itoh T., Fujita N., Kanno E., Yamamoto A., Yoshimori T., Fukuda M.
    Mol. Biol. Cell 19:2916-2925(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAB33B.
  6. Cited for: SUBCELLULAR LOCATION.
  7. Cited for: FUNCTION IN AUTOPHAGY, CLEAVAGE BY CASP3, MUTAGENESIS OF ASP-299 AND THR-300.

Entry informationi

Entry nameiA16L1_MOUSE
AccessioniPrimary (citable) accession number: Q8C0J2
Secondary accession number(s): Q6KAT7
, Q80U97, Q80U98, Q80U99, Q80Y53, Q9DB63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi