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Protein

Autophagy-related protein 16-1

Gene

Atg16l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.3 Publications

GO - Biological processi

  • autophagosome assembly Source: MGI
  • macroautophagy Source: Reactome
  • negative stranded viral RNA replication Source: MGI
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 16-1
Alternative name(s):
APG16-like 1
Gene namesi
Name:Atg16l1
Synonyms:Apg16l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1924290. Atg16l1.

Subcellular locationi

GO - Cellular componenti

  • autophagosome Source: MGI
  • autophagosome membrane Source: MGI
  • axoneme Source: UniProtKB
  • cytosol Source: Reactome
  • pre-autophagosomal structure membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi299D → E: Prevents cleavage by activated CASP3. 1 Publication1
Mutagenesisi300T → A: No effect on the stability of the protein. Enhances cleavage and degradation mediated by activated CASP3. Results in reduced autophagy and defective clearance of intestinal pathogens. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508491 – 607Autophagy-related protein 16-1Add BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei269PhosphoserineCombined sources1
Modified residuei287PhosphoserineCombined sources1

Post-translational modificationi

Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8C0J2.
PeptideAtlasiQ8C0J2.
PRIDEiQ8C0J2.

2D gel databases

REPRODUCTION-2DPAGEQ8C0J2.

PTM databases

iPTMnetiQ8C0J2.
PhosphoSitePlusiQ8C0J2.

Expressioni

Tissue specificityi

Widely expressed. In the liver, isoform 2 is highly expressed and isoform 1 is weakly expressed. Isoform 3 is expressed in the brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000026289.
CleanExiMM_ATG16L1.
ExpressionAtlasiQ8C0J2. baseline and differential.
GenevisibleiQ8C0J2. MM.

Interactioni

Subunit structurei

Interacts with TMEM59, TLR2 and NOD2 (By similarity). Homooligomer (PubMed:12665549). Interacts with WIPI2 and FIP200 (PubMed:24954904). Interacts with ATG5 (PubMed:12665549). Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B (PubMed:18448665). Interacts (via WD repeats) with MEFV (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Atg5Q99J834EBI-769195,EBI-2911848
Rb1cc1Q9ESK93EBI-769195,EBI-647302

Protein-protein interaction databases

BioGridi218476. 7 interactors.
DIPiDIP-31966N.
IntActiQ8C0J2. 7 interactors.
MINTiMINT-136268.
STRINGi10090.ENSMUSP00000108815.

Structurei

3D structure databases

ProteinModelPortaliQ8C0J2.
SMRiQ8C0J2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati320 – 359WD 1PROSITE-ProRule annotationAdd BLAST40
Repeati364 – 403WD 2PROSITE-ProRule annotationAdd BLAST40
Repeati406 – 445WD 3PROSITE-ProRule annotationAdd BLAST40
Repeati447 – 484WD 4PROSITE-ProRule annotationAdd BLAST38
Repeati486 – 525WD 5PROSITE-ProRule annotationAdd BLAST40
Repeati532 – 573WD 6PROSITE-ProRule annotationAdd BLAST42
Repeati575 – 607WD 7PROSITE-ProRule annotationAdd BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 230WIPI2-bindingBy similarityAdd BLAST24
Regioni230 – 242FIP200-bindingBy similarityAdd BLAST13

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili79 – 230Sequence analysisAdd BLAST152

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi296 – 299Caspase cleavage4

Sequence similaritiesi

Belongs to the WD repeat ATG16 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0288. Eukaryota.
ENOG410XQYA. LUCA.
GeneTreeiENSGT00840000129769.
HOVERGENiHBG050534.
InParanoidiQ8C0J2.
KOiK17890.
OMAiYVFDAHD.
PhylomeDBiQ8C0J2.
TreeFamiTF315541.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR013923. Autophagy-rel_prot_16.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08614. ATG16. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C0J2-1) [UniParc]FASTAAdd to basket
Also known as: Apg16Lbeta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSGLRAADF PRWKRHIAEE LRRRDRLQRQ AFEEIILQYT KLLEKSDLHS
60 70 80 90 100
VLTQKLQAEK HDMPNRHEIS PGHDGAWNDS QLQEMAQLRI KHQEELTELH
110 120 130 140 150
KKRGELAQLV IDLNNQMQQK DKEIQMNEAK ISEYLQTISD LETNCLDLRT
160 170 180 190 200
KLQDLEVANQ TLKDEYDALQ ITFTALEEKL RKTTEENQEL VTRWMAEKAQ
210 220 230 240 250
EANRLNAENE KDSRRRQARL QKELAEAAKE PLPVEQDDDI EVIVDETSDH
260 270 280 290 300
TEETSPVRAV SRAATKRLSQ PAGGLLDSIT NIFGRRSVSS IPVPQDIMDT
310 320 330 340 350
HPASGKDVRV PTTASYVFDA HDGEVNAVQF SPGSRLLATG GMDRRVKLWE
360 370 380 390 400
AFGDKCEFKG SLSGSNAGIT SIEFDSAGAY LLAASNDFAS RIWTVDDYRL
410 420 430 440 450
RHTLTGHSGK VLSAKFLLDN ARIVSGSHDR TLKLWDLRSK VCIKTVFAGS
460 470 480 490 500
SCNDIVCTEQ CVMSGHFDKK IRFWDIRSES VVREMELLGK ITALDLNPER
510 520 530 540 550
TELLSCSRDD LLKVIDLRTN AVKQTFSAPG FKCGSDWTRV VFSPDGSYVA
560 570 580 590 600
AGSAEGSLYV WSVLTGKVEK VLSKQHSSSI NAVAWAPSGL HVVSVDKGSR

AVLWAQP
Length:607
Mass (Da):68,172
Last modified:March 1, 2003 - v1
Checksum:i63920320883A69DF
GO
Isoform 2 (identifier: Q8C0J2-2) [UniParc]FASTAAdd to basket
Also known as: Apg16Lalpha

The sequence of this isoform differs from the canonical sequence as follows:
     266-284: Missing.

Note: No experimental confirmation available.
Show »
Length:588
Mass (Da):66,203
Checksum:iCE2AB829A7911274
GO
Isoform 3 (identifier: Q8C0J2-3) [UniParc]FASTAAdd to basket
Also known as: Apg16Lgamma

The sequence of this isoform differs from the canonical sequence as follows:
     284-284: G → GLSESPLLGHHSSDAAR

Show »
Length:623
Mass (Da):69,831
Checksum:i823453BB5D7DE6FE
GO
Isoform 4 (identifier: Q8C0J2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     284-284: G → GLSESPLLGHHSSDAAR
     443-452: IKTVFAGSSC → EEMQSLCVFM
     453-607: Missing.

Note: No experimental confirmation available.
Show »
Length:468
Mass (Da):53,024
Checksum:iFFB7AC4AE34DF8F7
GO
Isoform 5 (identifier: Q8C0J2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     266-284: Missing.
     442-442: C → CEEMQSLCVLMVFGFLSG

Note: No experimental confirmation available.
Show »
Length:605
Mass (Da):68,075
Checksum:iB0BB8DE0748401B6
GO

Sequence cautioni

The sequence BAD21370 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83Q → H in BAC55090 (PubMed:12665549).Curated1
Sequence conflicti83Q → H in BAC55091 (PubMed:12665549).Curated1
Sequence conflicti83Q → H in BAC55092 (PubMed:12665549).Curated1
Sequence conflicti83Q → H in BAB23866 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013391266 – 284Missing in isoform 2 and isoform 5. 2 PublicationsAdd BLAST19
Alternative sequenceiVSP_013392284G → GLSESPLLGHHSSDAAR in isoform 3 and isoform 4. 3 Publications1
Alternative sequenceiVSP_013393442C → CEEMQSLCVLMVFGFLSG in isoform 5. 1 Publication1
Alternative sequenceiVSP_013394443 – 452IKTVFAGSSC → EEMQSLCVFM in isoform 4. 1 Publication10
Alternative sequenceiVSP_013395453 – 607Missing in isoform 4. 1 PublicationAdd BLAST155

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB087879 mRNA. Translation: BAC55090.1.
AB087880 mRNA. Translation: BAC55091.1.
AB087881 mRNA. Translation: BAC55092.1.
AK005181 mRNA. Translation: BAB23866.1.
AK030983 mRNA. Translation: BAC27201.1.
AK131120 Transcribed RNA. Translation: BAD21370.1. Different initiation.
BC049122 mRNA. Translation: AAH49122.1.
CCDSiCCDS15136.1. [Q8C0J2-1]
CCDS56637.1. [Q8C0J2-3]
CCDS56638.1. [Q8C0J2-2]
RefSeqiNP_001192320.1. NM_001205391.1. [Q8C0J2-3]
NP_084122.2. NM_029846.4. [Q8C0J2-1]
UniGeneiMm.272972.
Mm.456099.

Genome annotation databases

EnsembliENSMUST00000027512; ENSMUSP00000027512; ENSMUSG00000026289. [Q8C0J2-1]
ENSMUST00000113186; ENSMUSP00000108811; ENSMUSG00000026289. [Q8C0J2-2]
ENSMUST00000113190; ENSMUSP00000108815; ENSMUSG00000026289. [Q8C0J2-3]
GeneIDi77040.
KEGGimmu:77040.
UCSCiuc007bxl.2. mouse. [Q8C0J2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB087879 mRNA. Translation: BAC55090.1.
AB087880 mRNA. Translation: BAC55091.1.
AB087881 mRNA. Translation: BAC55092.1.
AK005181 mRNA. Translation: BAB23866.1.
AK030983 mRNA. Translation: BAC27201.1.
AK131120 Transcribed RNA. Translation: BAD21370.1. Different initiation.
BC049122 mRNA. Translation: AAH49122.1.
CCDSiCCDS15136.1. [Q8C0J2-1]
CCDS56637.1. [Q8C0J2-3]
CCDS56638.1. [Q8C0J2-2]
RefSeqiNP_001192320.1. NM_001205391.1. [Q8C0J2-3]
NP_084122.2. NM_029846.4. [Q8C0J2-1]
UniGeneiMm.272972.
Mm.456099.

3D structure databases

ProteinModelPortaliQ8C0J2.
SMRiQ8C0J2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218476. 7 interactors.
DIPiDIP-31966N.
IntActiQ8C0J2. 7 interactors.
MINTiMINT-136268.
STRINGi10090.ENSMUSP00000108815.

PTM databases

iPTMnetiQ8C0J2.
PhosphoSitePlusiQ8C0J2.

2D gel databases

REPRODUCTION-2DPAGEQ8C0J2.

Proteomic databases

PaxDbiQ8C0J2.
PeptideAtlasiQ8C0J2.
PRIDEiQ8C0J2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027512; ENSMUSP00000027512; ENSMUSG00000026289. [Q8C0J2-1]
ENSMUST00000113186; ENSMUSP00000108811; ENSMUSG00000026289. [Q8C0J2-2]
ENSMUST00000113190; ENSMUSP00000108815; ENSMUSG00000026289. [Q8C0J2-3]
GeneIDi77040.
KEGGimmu:77040.
UCSCiuc007bxl.2. mouse. [Q8C0J2-1]

Organism-specific databases

CTDi55054.
MGIiMGI:1924290. Atg16l1.

Phylogenomic databases

eggNOGiKOG0288. Eukaryota.
ENOG410XQYA. LUCA.
GeneTreeiENSGT00840000129769.
HOVERGENiHBG050534.
InParanoidiQ8C0J2.
KOiK17890.
OMAiYVFDAHD.
PhylomeDBiQ8C0J2.
TreeFamiTF315541.

Enzyme and pathway databases

ReactomeiR-MMU-1632852. Macroautophagy.

Miscellaneous databases

PROiQ8C0J2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026289.
CleanExiMM_ATG16L1.
ExpressionAtlasiQ8C0J2. baseline and differential.
GenevisibleiQ8C0J2. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR013923. Autophagy-rel_prot_16.
IPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08614. ATG16. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA16L1_MOUSE
AccessioniPrimary (citable) accession number: Q8C0J2
Secondary accession number(s): Q6KAT7
, Q80U97, Q80U98, Q80U99, Q80Y53, Q9DB63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.