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Q8C0D4 (RHG12_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GTPase-activating protein 12
Alternative name(s):
Rho-type GTPase-activating protein 12
Gene names
Name:Arhgap12
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state By similarity.

Sequence similarities

Contains 1 PH domain.

Contains 1 Rho-GAP domain.

Contains 1 SH3 domain.

Contains 2 WW domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 838838Rho GTPase-activating protein 12
PRO_0000056715

Regions

Domain10 – 7263SH3
Domain263 – 29634WW 1
Domain355 – 38834WW 2
Domain466 – 567102PH
Domain648 – 836189Rho-GAP

Amino acid modifications

Modified residue1631Phosphoserine By similarity
Modified residue1991Phosphoserine Ref.6
Modified residue2041Phosphoserine Ref.6
Modified residue2111Phosphoserine By similarity
Modified residue2131Phosphoserine By similarity
Modified residue2291Phosphothreonine Ref.4 Ref.6
Modified residue2381Phosphoserine Ref.3 Ref.4 Ref.6
Modified residue2411Phosphotyrosine Ref.3 Ref.4
Modified residue5131Phosphothreonine Ref.5
Modified residue5841Phosphoserine Ref.2

Experimental info

Sequence conflict421K → N in BAC27494. Ref.1
Sequence conflict861P → A in BAC36587. Ref.1
Sequence conflict4831A → P in BAC36587. Ref.1
Sequence conflict5301S → T in BAC36587. Ref.1
Sequence conflict6981A → T in BAC36587. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8C0D4 [UniParc].

Last modified May 10, 2004. Version 2.
Checksum: C8660927694567AB

FASTA83895,352
        10         20         30         40         50         60 
MAERSGKITA GQAYIEVEYD YEYDAKDRKI VIRQGERYLL VKKTNDDWWQ VRPDENSKAF 

        70         80         90        100        110        120 
YVPAQYVKEV TRKALMPPVK QATGLPNNSM KTIQSMHLQR STENVNKMPE LSSFGKPSSS 

       130        140        150        160        170        180 
VQGTGLIRDA NQNFGSNYNS GQTLNLSLDL THNNGKFNSD SHSPKVSSQN RTRLFGHFPG 

       190        200        210        220        230        240 
PEFLDIEKTS FSQEQSCDSA GEGSERIQQD SESGDELSSS STEQMRATTP PNQGRPDSPV 

       250        260        270        280        290        300 
YANLQELKIS QSALPPLPGS PAIQVNGEWE THKDSSGRCY YYNRTTQERT WKPPRWARDV 

       310        320        330        340        350        360 
STSRDFQSPG EQEPLSSEEN YHSSCFSQSD SQCGSPPRGW SEELDERGHT LYTSDYTKEK 

       370        380        390        400        410        420 
WLKHVDDQGR QYYYSADGSR SEWELPKYNA SSQQQREIIK SRSLDRRLQE PIVLTKWRHS 

       430        440        450        460        470        480 
TIVLDSNDKD SPTTTKLCLP ENESPPTSSK HQDPGQEKYG LLNVTKITEN GKKVRKNWLS 

       490        500        510        520        530        540 
SWAVLQGSSL LFTKTQGSST SWFGSNQSKP EFTVDLKGAV IEMASKDKSS KKNVFELKTR 

       550        560        570        580        590        600 
QGTELLIQSD NDAVINDWFK VLSSTINNQV AEADEAAEEE TPDSPGVEKH DKEKDQKELK 

       610        620        630        640        650        660 
KLRSMKGSSM DSSEQKKTKK NLKKFLTRRP TLQAVREKGY IKDQVFGSNL ANLCQRENGT 

       670        680        690        700        710        720 
VPKFVKLCIE HVEEHGLDVD GIYRVSGNLA VIQKLRFAVN HDEKLDLNDS KWEDIHVITG 

       730        740        750        760        770        780 
ALKMFFRELP EPLFTFNHFN DFVNAIKQEP RQRVTAVKDL IRQLPKPNQD TMQILFRHLK 

       790        800        810        820        830 
RVIENGEKNR MTYQSIAIVF GPTLLKPERE TGNIAVHTVY QNQIVELILL ELSTVFGR

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Testis.
[2]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-584, MASS SPECTROMETRY.
Tissue: Embryonic brain.
[3]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238 AND TYR-241, MASS SPECTROMETRY.
Tissue: Mast cell.
[4]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-229; SER-238 AND TYR-241, MASS SPECTROMETRY.
Tissue: Brain.
[5]"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry."
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.
J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-513, MASS SPECTROMETRY.
Tissue: Liver.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199; SER-204; THR-229 AND SER-238, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK031648 mRNA. Translation: BAC27494.1.
AK077063 mRNA. Translation: BAC36587.1.
IPIIPI00223751.
RefSeqNP_001034781.1. NM_001039692.1.
NP_083553.2. NM_029277.2.
UniGeneMm.239675.

3D structure databases

ProteinModelPortalQ8C0D4.
SMRQ8C0D4. Positions 11-70, 457-566, 629-837.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000054209.

PTM databases

PhosphoSiteQ8C0D4.

Proteomic databases

PaxDbQ8C0D4.
PRIDEQ8C0D4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000062584; ENSMUSP00000054209; ENSMUSG00000041225.
GeneID75415.
KEGGmmu:75415.
UCSCuc008dzc.1. mouse.

Organism-specific databases

CTD94134.
MGIMGI:1922665. Arhgap12.

Phylogenomic databases

eggNOGNOG279563.
GeneTreeENSGT00700000104029.
HOGENOMHOG000294167.
HOVERGENHBG005328.
InParanoidQ8C0D4.
OMAGQVYIEV.

Gene expression databases

ArrayExpressQ8C0D4.
BgeeQ8C0D4.
CleanExMM_ARHGAP12.
GenevestigatorQ8C0D4.
GermOnlineENSMUSG00000041225. Mus musculus.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR015767. Rho_GTPase_activating.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
IPR001202. WW_dom.
[Graphical view]
PANTHERPTHR23181. PTHR23181. 1 hit.
PfamPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMSSF48350. Rho_GAP. 1 hit.
SSF50044. SH3. 1 hit.
SSF51045. WW_Rsp5_WWP. 2 hits.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
PS01159. WW_DOMAIN_1. False negative.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio342942.
SOURCESearch...

Entry information

Entry nameRHG12_MOUSE
AccessionPrimary (citable) accession number: Q8C0D4
Secondary accession number(s): Q8BVP8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents