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Protein

Probable tRNA pseudouridine synthase 1

Gene

Trub1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be responsible for synthesis of pseudouridine from uracil in transfer RNAs.By similarity

Catalytic activityi

tRNA uridine = tRNA pseudouridine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei109NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA pseudouridine synthase 1 (EC:5.4.99.-)
Gene namesi
Name:Trub1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1919383 Trub1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002520882 – 338Probable tRNA pseudouridine synthase 1Add BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8C0D0
PaxDbiQ8C0D0
PRIDEiQ8C0D0

PTM databases

iPTMnetiQ8C0D0
PhosphoSitePlusiQ8C0D0

Expressioni

Gene expression databases

BgeeiENSMUSG00000025086
GenevisibleiQ8C0D0 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026073

Structurei

3D structure databases

ProteinModelPortaliQ8C0D0
SMRiQ8C0D0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated

Phylogenomic databases

eggNOGiKOG2529 Eukaryota
COG0130 LUCA
GeneTreeiENSGT00530000063611
HOGENOMiHOG000063679
HOVERGENiHBG094094
InParanoidiQ8C0D0
KOiK03177
OMAiKFQPPFF
OrthoDBiEOG091G0FSQ
PhylomeDBiQ8C0D0
TreeFamiTF320759

Family and domain databases

HAMAPiMF_01080 TruB_bact, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR002501 PsdUridine_synth_N
IPR014780 tRNA_psdUridine_synth_TruB
PANTHERiPTHR13767 PTHR13767, 1 hit
PfamiView protein in Pfam
PF01509 TruB_N, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00431 TruB, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C0D0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAEAEVVS PLIVDTAPDT SGTAEASVAA SVAEAARTES QAPASKAALA
60 70 80 90 100
AKLMSLSGVF AVHKPKGPTS AELLNRLKEK LLAEAGMPSP EWNKRQKQTL
110 120 130 140 150
KVGHGGTLDS AAQGVLVVGI GRGTKMLTSM LSGSKRYITI GELGKATDTL
160 170 180 190 200
DSTGKVTEEK PYDKITREDI EGILQKFTGN IMQVPPLYSA LKKDGQRLST
210 220 230 240 250
LMKKGKVVEA RPARPVTVHS ISLLKFQPPF FTLDVECGGG FYIRSLVSDI
260 270 280 290 300
GKELSSCASV LELTRTKQGP FTLAQHALPE DRWTIDDIEQ SLERCTSLLP
310 320 330
EELTFKKLKS DNSSDQVISC GYITLRDTKR EDDAVKTL
Length:338
Mass (Da):36,348
Last modified:March 1, 2003 - v1
Checksum:iB76CFC4381A6772A
GO
Isoform 2 (identifier: Q8C0D0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     234-247: DVECGGGFYIRSLV → GFLCIALAVLELTL
     248-338: Missing.

Note: No experimental confirmation available.
Show »
Length:247
Mass (Da):26,096
Checksum:iA48B80BA176EF544
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60F → L in BAB31254 (PubMed:16141072).Curated1
Sequence conflicti74L → P in AAH89592 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020860234 – 247DVECG…IRSLV → GFLCIALAVLELTL in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_020861248 – 338Missing in isoform 2. 1 PublicationAdd BLAST91

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018525 mRNA Translation: BAB31254.1
AK031674 mRNA Translation: BAC27506.1
AK169221 mRNA Translation: BAE40991.1
BC089592 mRNA Translation: AAH89592.1
CCDSiCCDS29926.1 [Q8C0D0-1]
CCDS29927.1 [Q8C0D0-2]
RefSeqiNP_082391.1, NM_028115.3 [Q8C0D0-1]
NP_084115.2, NM_029839.3 [Q8C0D0-2]
UniGeneiMm.258404

Genome annotation databases

EnsembliENSMUST00000026072; ENSMUSP00000026072; ENSMUSG00000025086 [Q8C0D0-2]
ENSMUST00000026073; ENSMUSP00000026073; ENSMUSG00000025086 [Q8C0D0-1]
GeneIDi72133
KEGGimmu:72133
UCSCiuc008iae.2 mouse [Q8C0D0-2]
uc008iag.2 mouse [Q8C0D0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRUB1_MOUSE
AccessioniPrimary (citable) accession number: Q8C0D0
Secondary accession number(s): Q5EBH7, Q9D331
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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