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Protein

Phenylalanine--tRNA ligase alpha subunit

Gene

Farsa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei329 – 3291L-phenylalanineBy similarity
Binding sitei435 – 4351L-phenylalanineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase alpha subunit (EC:6.1.1.20By similarity)
Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name:
PheRS
Gene namesi
Name:Farsa
Synonyms:Farsla
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1913840. Farsa.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 508507Phenylalanine--tRNA ligase alpha subunitPRO_0000280447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei193 – 1931PhosphoserineBy similarity
Modified residuei301 – 3011PhosphoserineCombined sources
Modified residuei311 – 3111N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8C0C7.
MaxQBiQ8C0C7.
PaxDbiQ8C0C7.
PeptideAtlasiQ8C0C7.
PRIDEiQ8C0C7.

PTM databases

iPTMnetiQ8C0C7.
PhosphoSiteiQ8C0C7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003808.
CleanExiMM_FARSA.
ExpressionAtlasiQ8C0C7. baseline and differential.
GenevisibleiQ8C0C7. MM.

Interactioni

Subunit structurei

Heterotetramer; dimer of two heterodimers formed by FARSA and FARSB.By similarity

Protein-protein interaction databases

BioGridi211576. 2 interactions.
IntActiQ8C0C7. 6 interactions.
MINTiMINT-4113807.
STRINGi10090.ENSMUSP00000003906.

Structurei

3D structure databases

ProteinModelPortaliQ8C0C7.
SMRiQ8C0C7. Positions 1-508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni372 – 3743L-phenylalanine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2784. Eukaryota.
COG0016. LUCA.
GeneTreeiENSGT00390000006387.
HOGENOMiHOG000230294.
HOVERGENiHBG068046.
InParanoidiQ8C0C7.
KOiK01889.
OMAiYKYTWKL.
OrthoDBiEOG091G04JI.
PhylomeDBiQ8C0C7.
TreeFamiTF300647.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8C0C7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNPVLELL LRRLEVADGG LDSAELATQL GVEHQAVVGA VKSLQALGEV
60 70 80 90 100
IEAELRSTKC WELTTEGEEI AREGSHEARV FRSIPLEGLV QSELMHLPSG
110 120 130 140 150
KVGFSKAMSN KWIRVDKSAA DGPRVFRVVD SIEDEVQKRL QLVQAGQAEK
160 170 180 190 200
LAEKERNELR KRKLLTEVIL KTYWVSKGKA FSTSVSKQEA ELSPEMISSG
210 220 230 240 250
SWRDRPFKPY NFSARGVLPD SGHLHPLLKV RSQFRQIFLE MGFTEMPTDN
260 270 280 290 300
FIESSFWNFD ALFQPQQHPA RDQHDTFFLR DPAEALQLPM GYVQRVKRTH
310 320 330 340 350
SQGGYGSQGY KYTWKLEEAR KNLLRTHTTA ASARALYQLA QKKPFTPAKY
360 370 380 390 400
FSIDRVFRNE TLDATHLAEF HQIEGVIADH GLTLGHLMGV LREFFTKLGI
410 420 430 440 450
TQLRFKPAYN PYTEPSMEVF SYHQGLKKWV EVGNSGVFRP EMLLPMGLPE
460 470 480 490 500
NVSVIAWGLS LERPTMIKYG INNIRELVGH KVNLQMVYDS PVCRLDIEPR

SSKTQEAA
Length:508
Mass (Da):57,599
Last modified:March 1, 2003 - v1
Checksum:i9C125BC4D4A26DAA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti291 – 2911G → D in AAH06862 (PubMed:15489334).Curated
Sequence conflicti291 – 2911G → D in AAH13533 (PubMed:15489334).Curated
Sequence conflicti343 – 3431Missing in AAH13533 (PubMed:15489334).Curated
Sequence conflicti455 – 4551I → T in AAH06862 (PubMed:15489334).Curated
Sequence conflicti455 – 4551I → V in BAE32726 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031697 mRNA. Translation: BAC27519.1.
AK154629 mRNA. Translation: BAE32726.1.
BC006862 mRNA. Translation: AAH06862.1.
BC013533 mRNA. Translation: AAH13533.1.
CCDSiCCDS22480.2.
RefSeqiNP_079924.2. NM_025648.3.
UniGeneiMm.292517.

Genome annotation databases

EnsembliENSMUST00000003906; ENSMUSP00000003906; ENSMUSG00000003808.
GeneIDi66590.
KEGGimmu:66590.
UCSCiuc009mnt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031697 mRNA. Translation: BAC27519.1.
AK154629 mRNA. Translation: BAE32726.1.
BC006862 mRNA. Translation: AAH06862.1.
BC013533 mRNA. Translation: AAH13533.1.
CCDSiCCDS22480.2.
RefSeqiNP_079924.2. NM_025648.3.
UniGeneiMm.292517.

3D structure databases

ProteinModelPortaliQ8C0C7.
SMRiQ8C0C7. Positions 1-508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211576. 2 interactions.
IntActiQ8C0C7. 6 interactions.
MINTiMINT-4113807.
STRINGi10090.ENSMUSP00000003906.

PTM databases

iPTMnetiQ8C0C7.
PhosphoSiteiQ8C0C7.

Proteomic databases

EPDiQ8C0C7.
MaxQBiQ8C0C7.
PaxDbiQ8C0C7.
PeptideAtlasiQ8C0C7.
PRIDEiQ8C0C7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003906; ENSMUSP00000003906; ENSMUSG00000003808.
GeneIDi66590.
KEGGimmu:66590.
UCSCiuc009mnt.2. mouse.

Organism-specific databases

CTDi2193.
MGIiMGI:1913840. Farsa.

Phylogenomic databases

eggNOGiKOG2784. Eukaryota.
COG0016. LUCA.
GeneTreeiENSGT00390000006387.
HOGENOMiHOG000230294.
HOVERGENiHBG068046.
InParanoidiQ8C0C7.
KOiK01889.
OMAiYKYTWKL.
OrthoDBiEOG091G04JI.
PhylomeDBiQ8C0C7.
TreeFamiTF300647.

Miscellaneous databases

ChiTaRSiFarsa. mouse.
PROiQ8C0C7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003808.
CleanExiMM_FARSA.
ExpressionAtlasiQ8C0C7. baseline and differential.
GenevisibleiQ8C0C7. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR006195. aa-tRNA-synth_II.
IPR004529. Phe-tRNA-synth_IIc_asu.
IPR002319. Phenylalanyl-tRNA_Synthase.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01409. tRNA-synt_2d. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
TIGRFAMsiTIGR00468. pheS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYFA_MOUSE
AccessioniPrimary (citable) accession number: Q8C0C7
Secondary accession number(s): Q3U3Q9, Q91WR4, Q922S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.