Reviewed, UniProtKB/Swiss-Prot Q8C0C0 (ZHX2_MOUSE)

Names and origin

Protein names

Recommended name:
    Zinc fingers and homeoboxes protein 2
Alternative name(s):
    Zinc finger and homeodomain protein 2
    Alpha-fetoprotein regulator 1
      Short name=AFP regulator 1
    Regulator of AFP

Gene names

Name:	Zhx2
Synonyms:	Afr1, Raf

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	836 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a transcriptional repressor By similarity. UniProtKB Q9Y6X8
Subunit structure	Forms homodimers. Also forms heterodimers with ZHX1 and ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts with NFYA. Ref.1 UniProtKB Q9Y6X8
Subcellular location	Nucleus By similarity.
Tissue specificity	Ubiquitously expressed. Ref.1
Sequence similarities	Belongs to the ZHX family. Contains 2 C2H2-type zinc fingers. Contains 4 homeobox DNA-binding domains.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	mRNA catabolic process Inferred from direct assay. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 836	836	Zinc fingers and homeoboxes protein 2		PRO_0000049392
Regions
	Zinc finger	78 – 101	24	C2H2-type 1
	Zinc finger	110 – 133	24	C2H2-type 2
	DNA binding	263 – 324	62	Homeobox 1
	DNA binding	439 – 501	63	Homeobox 2
	DNA binding	530 – 591	62	Homeobox 3
	DNA binding	628 – 690	63	Homeobox 4
	Region	195 – 358	164	Required for homodimerization By similarity UniProtKB Q9Y6X8
	Region	263 – 497	235	Required for interaction with NFYA By similarity UniProtKB Q9Y6X8
	Region	263 – 446	184	Required for repressor activity By similarity UniProtKB Q9Y6X8
	Region	317 – 446	130	Required for nuclear localization By similarity UniProtKB Q9Y6X8
Amino acid modifications
	Modified residue	16	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	70	1	L → P in BAC87710. Ref.1
	Sequence conflict	70	1	L → P in BAC87712. Ref.1
	Sequence conflict	699	1	M → L in BAC87710. Ref.1
	Sequence conflict	699	1	M → L in BAC87712. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8C0C0-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C28AF20925D62054	FASTA	836	92,259
	10 20 30 40 50 60 MASKRKSTTP CMVRTSQVLE QDMLEEADRA KDKGAGMPQS DVTKDSWAAE PEHSSKETEV 70 80 90 100 110 120 VEVKSMGENL SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ HPNVILNPLY VCAECNFTTK 130 140 150 160 170 180 KYDSLSDHNS KFHPGETNFK LKLIKRNNQT VLEQSIEATN HVVPITASGP GSSDNDPGVS 190 200 210 220 230 240 VGKTPMTKTG KLKADAKKVP KKPDEAAPEN HMEGTARLVT DTAEILARLG SVELLQDSLG 250 260 270 280 290 300 HVMPSVQLPP NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK 310 320 330 340 350 360 HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA QLTPTKVSQP 370 380 390 400 410 420 ILQTALPCQI LGQPSLVLTQ VTSGSTTVSC SPITLAVAGV TNHGQKRPLV TPQAAPEPKR 430 440 450 460 470 480 PHIAQVPEPP PKVANTPLTP ASDRKKTKLQ IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR 490 500 510 520 530 540 SEIKKWFSDH RYRCQRGIVH ITSESLAKDQ MAITGTRHGR TYHVYPDFAP QKFKEKSQGQ 550 560 570 580 590 600 LKTLEDSFLK SSFPTQAEVE RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK 610 620 630 640 650 660 GSDAVAPNGA LSRLDQLSGA QLAGSLPSPS SAIVQNQEQV HLLRSTFART QWPTPQEYDQ 670 680 690 700 710 720 LAAKTGLVRT EIVRWFKENR CLLKTGTLSW LEQYQRHHMS DDRGRDAVSR KVAKQVAESP 730 740 750 760 770 780 KNGSEAAHQY AKDPKALSEE DSEKLVPRMK VGGDPTKDCL AGKPSEATSD RSEGSRDGQG 790 800 810 820 830 SEENEESGIV DFVEVTVGEE DAISEKWGSW SRRVAEGTVE RADSDSDSTP AEAGQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse zinc-fingers and homeoboxes (ZHX) family: ZHX2 forms a heterodimer with ZHX3." Kawata H., Yamada K., Shou Z., Mizutani T., Miyamoto K. Gene 323:133-140(2003) [PubMed: 14659886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, HETERODIMERIZATION WITH ZHX3. Strain: 129.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line." Shu H., Chen S., Bi Q., Mumby M., Brekken D.L. Mol. Cell. Proteomics 3:279-286(2004) [PubMed: 14729942] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY. Tissue: B-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB099526 mRNA. Translation: BAC87710.1. AB099704 Genomic DNA. Translation: BAC87712.1. AK031782 mRNA. Translation: BAC27546.1. BC059178 mRNA. Translation: AAH59178.1.
IPI	IPI00399681.
RefSeq	NP_955520.1.
UniGene	Mm.36557
3D structure databases
SMR	Q8C0C0. Positions 525-593.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8C0C0. 1 interaction.
STRING	Q8C0C0.
PTM databases
PhosphoSite	Q8C0C0.
Proteomic databases
PRIDE	Q8C0C0.
Genome annotation databases
Ensembl	ENSMUST00000096430; ENSMUSP00000094164; ENSMUSG00000071757; Mus musculus. [Genome view]
GeneID	387609.
KEGG	mmu:387609.
UCSC	uc007vsp.1. mouse.
Organism-specific databases
CTD	387609.
MGI	MGI:2683087. Zhx2.
Phylogenomic databases
HOGENOM	Q8C0C0.
HOVERGEN	Q8C0C0.
OMA	ATQRLKH
OrthoDB	EOG96MF42
Gene expression databases
ArrayExpress	Q8C0C0.
Bgee	Q8C0C0.
CleanEx	MM_ZHX2.
Genevestigator	Q8C0C0.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 3 hits.
Pfam	PF00046. Homeobox. 3 hits. [Graphical view]
SMART	SM00389. HOX. 4 hits. SM00355. ZnF_C2H2. 2 hits. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. False negative. PS50071. HOMEOBOX_2. 3 hits. PS00028. ZINC_FINGER_C2H2_1. False negative. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	405958.
SOURCE	Search...

Entry information

Entry name

ZHX2_MOUSE

Accession

Primary (citable) accession number: Q8C0C0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2003
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)