Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc fingers and homeoboxes protein 2

Gene

Zhx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Represses the promoter activity of the CDC25C gene stimulated by NFYA (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri78 – 10124C2H2-type 1PROSITE-ProRule annotationCuratedAdd
BLAST
Zinc fingeri110 – 13324C2H2-type 2PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi263 – 32462Homeobox 1PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi439 – 50163Homeobox 2PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi530 – 59162Homeobox 3PROSITE-ProRule annotationCuratedAdd
BLAST
DNA bindingi628 – 69063Homeobox 4PROSITE-ProRule annotationCuratedAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • mRNA catabolic process Source: MGI
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • somatic stem cell population maintenance Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc fingers and homeoboxes protein 2
Alternative name(s):
Alpha-fetoprotein regulator 1
Short name:
AFP regulator 1
Regulator of AFP
Zinc finger and homeodomain protein 2
Gene namesi
Name:Zhx2
Synonyms:Afr1, Raf
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2683087. Zhx2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 836836Zinc fingers and homeoboxes protein 2PRO_0000049392Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei824 – 8241PhosphoserineCombined sources
Modified residuei826 – 8261PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8C0C0.
MaxQBiQ8C0C0.
PaxDbiQ8C0C0.
PRIDEiQ8C0C0.

PTM databases

iPTMnetiQ8C0C0.
PhosphoSiteiQ8C0C0.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiQ8C0C0.
CleanExiMM_ZHX2.
GenevisibleiQ8C0C0. MM.

Interactioni

Subunit structurei

Forms a homodimer (via homeobox domain). Forms a heterodimer with ZHX1 (via HD1 domain). Forms a heterodimer with ZHX3. Heterodimerization with ZHX1 is not necessary for repressor activity. Interacts (via homeobox domain) with NFYA (via N-terminus) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dock1Q8BUR43EBI-646042,EBI-646023

GO - Molecular functioni

Protein-protein interaction databases

BioGridi239920. 2 interactions.
IntActiQ8C0C0. 1 interaction.
STRINGi10090.ENSMUSP00000094164.

Structurei

3D structure databases

ProteinModelPortaliQ8C0C0.
SMRiQ8C0C0. Positions 71-156, 446-501, 525-593, 638-695.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni195 – 358164Required for homodimerizationBy similarityAdd
BLAST
Regioni263 – 497235Required for interaction with NFYABy similarityAdd
BLAST
Regioni263 – 446184Required for repressor activityBy similarityAdd
BLAST
Regioni317 – 446130Required for nuclear localizationBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ZHX family.Curated
Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotationCurated
Contains 4 homeobox DNA-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri78 – 10124C2H2-type 1PROSITE-ProRule annotationCuratedAdd
BLAST
Zinc fingeri110 – 13324C2H2-type 2PROSITE-ProRule annotationCuratedAdd
BLAST

Keywords - Domaini

Homeobox, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOGENOMiHOG000013231.
HOVERGENiHBG007920.
InParanoidiQ8C0C0.
OMAiFKENRCL.
OrthoDBiEOG76HQ0T.
PhylomeDBiQ8C0C0.
TreeFamiTF333363.

Family and domain databases

Gene3Di1.10.10.60. 4 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 3 hits.
[Graphical view]
SMARTiSM00389. HOX. 4 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 4 hits.
PROSITEiPS50071. HOMEOBOX_2. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C0C0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKRKSTTP CMVRTSQVLE QDMLEEADRA KDKGAGMPQS DVTKDSWAAE
60 70 80 90 100
PEHSSKETEV VEVKSMGENL SKKLQGGYEC KYCPYSTQNL NEFTEHVDMQ
110 120 130 140 150
HPNVILNPLY VCAECNFTTK KYDSLSDHNS KFHPGETNFK LKLIKRNNQT
160 170 180 190 200
VLEQSIEATN HVVPITASGP GSSDNDPGVS VGKTPMTKTG KLKADAKKVP
210 220 230 240 250
KKPDEAAPEN HMEGTARLVT DTAEILARLG SVELLQDSLG HVMPSVQLPP
260 270 280 290 300
NINLVPKVPV PLNTTKYNSA LDTNATMINS FNKFPYPTQA ELSWLTAASK
310 320 330 340 350
HPEEHIRIWF ATQRLKHGIS WSPEEVEEAR KKMFNGTIQS VPPTITVLPA
360 370 380 390 400
QLTPTKVSQP ILQTALPCQI LGQPSLVLTQ VTSGSTTVSC SPITLAVAGV
410 420 430 440 450
TNHGQKRPLV TPQAAPEPKR PHIAQVPEPP PKVANTPLTP ASDRKKTKLQ
460 470 480 490 500
IAHLKASFLQ SQFPDDAEVY RLIEVTGLAR SEIKKWFSDH RYRCQRGIVH
510 520 530 540 550
ITSESLAKDQ MAITGTRHGR TYHVYPDFAP QKFKEKSQGQ LKTLEDSFLK
560 570 580 590 600
SSFPTQAEVE RLRVETKLSR REIDSWFSER RKLRDSMEQA VLDSMGSGKK
610 620 630 640 650
GSDAVAPNGA LSRLDQLSGA QLAGSLPSPS SAIVQNQEQV HLLRSTFART
660 670 680 690 700
QWPTPQEYDQ LAAKTGLVRT EIVRWFKENR CLLKTGTLSW LEQYQRHHMS
710 720 730 740 750
DDRGRDAVSR KVAKQVAESP KNGSEAAHQY AKDPKALSEE DSEKLVPRMK
760 770 780 790 800
VGGDPTKDCL AGKPSEATSD RSEGSRDGQG SEENEESGIV DFVEVTVGEE
810 820 830
DAISEKWGSW SRRVAEGTVE RADSDSDSTP AEAGQA
Length:836
Mass (Da):92,259
Last modified:March 1, 2003 - v1
Checksum:iC28AF20925D62054
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701L → P in BAC87710 (PubMed:14659886).Curated
Sequence conflicti70 – 701L → P in BAC87712 (PubMed:14659886).Curated
Sequence conflicti699 – 6991M → L in BAC87710 (PubMed:14659886).Curated
Sequence conflicti699 – 6991M → L in BAC87712 (PubMed:14659886).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB099526 mRNA. Translation: BAC87710.1.
AB099704 Genomic DNA. Translation: BAC87712.1.
AK031782 mRNA. Translation: BAC27546.1.
BC059178 mRNA. Translation: AAH59178.1.
CCDSiCCDS27483.1.
RefSeqiNP_955520.1. NM_199449.2.
XP_011243990.1. XM_011245688.1.
XP_011243991.1. XM_011245689.1.
UniGeneiMm.36557.

Genome annotation databases

EnsembliENSMUST00000096430; ENSMUSP00000094164; ENSMUSG00000071757.
GeneIDi387609.
KEGGimmu:387609.
UCSCiuc007vsp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB099526 mRNA. Translation: BAC87710.1.
AB099704 Genomic DNA. Translation: BAC87712.1.
AK031782 mRNA. Translation: BAC27546.1.
BC059178 mRNA. Translation: AAH59178.1.
CCDSiCCDS27483.1.
RefSeqiNP_955520.1. NM_199449.2.
XP_011243990.1. XM_011245688.1.
XP_011243991.1. XM_011245689.1.
UniGeneiMm.36557.

3D structure databases

ProteinModelPortaliQ8C0C0.
SMRiQ8C0C0. Positions 71-156, 446-501, 525-593, 638-695.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi239920. 2 interactions.
IntActiQ8C0C0. 1 interaction.
STRINGi10090.ENSMUSP00000094164.

PTM databases

iPTMnetiQ8C0C0.
PhosphoSiteiQ8C0C0.

Proteomic databases

EPDiQ8C0C0.
MaxQBiQ8C0C0.
PaxDbiQ8C0C0.
PRIDEiQ8C0C0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000096430; ENSMUSP00000094164; ENSMUSG00000071757.
GeneIDi387609.
KEGGimmu:387609.
UCSCiuc007vsp.1. mouse.

Organism-specific databases

CTDi22882.
MGIiMGI:2683087. Zhx2.

Phylogenomic databases

eggNOGiENOG410IHET. Eukaryota.
ENOG410Z8A2. LUCA.
GeneTreeiENSGT00530000063149.
HOGENOMiHOG000013231.
HOVERGENiHBG007920.
InParanoidiQ8C0C0.
OMAiFKENRCL.
OrthoDBiEOG76HQ0T.
PhylomeDBiQ8C0C0.
TreeFamiTF333363.

Miscellaneous databases

NextBioi405958.
PROiQ8C0C0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8C0C0.
CleanExiMM_ZHX2.
GenevisibleiQ8C0C0. MM.

Family and domain databases

Gene3Di1.10.10.60. 4 hits.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF00046. Homeobox. 3 hits.
[Graphical view]
SMARTiSM00389. HOX. 4 hits.
SM00355. ZnF_C2H2. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 4 hits.
PROSITEiPS50071. HOMEOBOX_2. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse zinc-fingers and homeoboxes (ZHX) family: ZHX2 forms a heterodimer with ZHX3."
    Kawata H., Yamada K., Shou Z., Mizutani T., Miyamoto K.
    Gene 323:133-140(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, HETERODIMERIZATION WITH ZHX3.
    Strain: 1291 Publication.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported.
    Tissue: BrainImported.
  4. "ZHX proteins regulate podocyte gene expression during the development of nephrotic syndrome."
    Liu G., Clement L.C., Kanwar Y.S., Avila-Casado C., Chugh S.S.
    J. Biol. Chem. 281:39681-39692(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Tissue: Kidney.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-824 AND SER-826, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiZHX2_MOUSE
AccessioniPrimary (citable) accession number: Q8C0C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2003
Last modified: March 16, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.