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Protein

Cholinephosphotransferase 1

Gene

Chpt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes phosphatidylcholine biosynthesis from CDP-choline. It thereby plays a central role in the formation and maintenance of vesicular membranes (By similarity).By similarity

Catalytic activityi

CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.

Cofactori

Mg2+By similarity, Mn2+By similarity

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (Pcyt1a), Choline-phosphate cytidylyltransferase B (Pcyt1b)
  2. Cholinephosphotransferase 1 (Chpt1), Choline/ethanolaminephosphotransferase 1 (Cept1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483191. Synthesis of PC.
UniPathwayiUPA00753; UER00740.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinephosphotransferase 1 (EC:2.7.8.2)
Short name:
mCHPT1
Alternative name(s):
Diabetic nephropathy-associated gene transcript 1
Diacylglycerol cholinephosphotransferase 1
Gene namesi
Name:Chpt1
Synonyms:Cpt1, Dn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2384841. Chpt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei66 – 8621HelicalSequence analysisAdd
BLAST
Transmembranei93 – 11321HelicalSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST
Transmembranei226 – 24823HelicalSequence analysisAdd
BLAST
Transmembranei261 – 28121HelicalSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 398397Cholinephosphotransferase 1PRO_0000289253Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8C025.
PaxDbiQ8C025.
PRIDEiQ8C025.

PTM databases

iPTMnetiQ8C025.
PhosphoSiteiQ8C025.

Expressioni

Tissue specificityi

Expressed in brain, heart, lung, liver, spleen, intestine and muscle. Down-regulated in kidney of type 2 diabetic KK/Ta mice.1 Publication

Gene expression databases

BgeeiENSMUSG00000060002.
CleanExiMM_CHPT1.
ExpressionAtlasiQ8C025. baseline and differential.
GenevisibleiQ8C025. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112708.

Structurei

3D structure databases

ProteinModelPortaliQ8C025.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
GeneTreeiENSGT00530000063048.
HOGENOMiHOG000230699.
HOVERGENiHBG107647.
InParanoidiQ8C025.
KOiK00994.
PhylomeDBiQ8C025.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8C025-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGAGARPA PRWVKALGEP LSAAQLRRLE EHRYTAVGES LFEPPLQLYW
60 70 80 90 100
TWLLQWIPLW MAPNTITLIG LAINLVTTLV LIFYCPTVTE EAPYWTYLLC
110 120 130 140 150
ALGLFIYQSL DAIDGKQARR TNSCSPLGEL FDHGCDSLST VFMAIGASIA
160 170 180 190 200
VRLGTHPDWL FFCSFVGMFM FYCAHWQTYV SGVLRFGRVD VTEIQVALVI
210 220 230 240 250
VFMLSTFGGA TMWDYTIPIL EIKLKIVPVL GVVGGLIFSC SNYFHVILHG
260 270 280 290 300
GVGKNGSTIA GTSVLSPGLH IGLIIILAIM IYKKSATNMF EKHPCLYTLM
310 320 330 340 350
FGCVFAKVAQ KLVIAHMTKS ELYLQDTVFI GPGLLFLDQY FNNFIDEYVV
360 370 380 390
LWIAMVISSF DMMIYFTSLC LQISRHLHLN IFKTSCQQAP EQVYKHID
Length:398
Mass (Da):44,608
Last modified:March 1, 2003 - v1
Checksum:i09DCC40C0F0D8CEF
GO
Isoform 2 (identifier: Q8C025-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     188-237: RVDVTEIQVA...PVLGVVGGLI → SQEVFFRAGE...TRSDALFWSV
     238-398: Missing.

Note: No experimental confirmation available.
Show »
Length:237
Mass (Da):26,517
Checksum:iEB774EAAE8891656
GO
Isoform 3 (identifier: Q8C025-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     188-229: RVDVTEIQVA...LEIKLKIVPV → RWRTLSSLSS...NQLQWNVFFL
     230-398: Missing.

Note: No experimental confirmation available.
Show »
Length:229
Mass (Da):25,739
Checksum:i5F040E5FE94EBB1A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281R → W in AAH16251 (PubMed:15489334).Curated
Sequence conflicti159 – 1591W → G in AAR16089 (PubMed:15254874).Curated
Sequence conflicti362 – 3621M → L in AAR16089 (PubMed:15254874).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei188 – 23750RVDVT…VGGLI → SQEVFFRAGEMAQWVRAPDC SSEGPEFKSQQPHGGSQPSV TRSDALFWSV in isoform 2. 1 PublicationVSP_025991Add
BLAST
Alternative sequencei188 – 22942RVDVT…KIVPV → RWRTLSSLSSTMPAACHHAS CHNDHGLNLGTLNQLQWNVF FL in isoform 3. 1 PublicationVSP_025992Add
BLAST
Alternative sequencei230 – 398169Missing in isoform 3. 1 PublicationVSP_025993Add
BLAST
Alternative sequencei238 – 398161Missing in isoform 2. 1 PublicationVSP_025994Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY445814 mRNA. Translation: AAR16089.1.
AK032497 mRNA. Translation: BAC27897.1.
BC016251 mRNA. Translation: AAH16251.1.
BC031435 mRNA. Translation: AAH31435.1.
CCDSiCCDS24112.1. [Q8C025-1]
PIRiS12207.
RefSeqiNP_659056.3. NM_144807.3. [Q8C025-1]
XP_006513518.1. XM_006513455.1. [Q8C025-2]
UniGeneiMm.288897.

Genome annotation databases

EnsembliENSMUST00000020253; ENSMUSP00000020253; ENSMUSG00000060002. [Q8C025-1]
ENSMUST00000073783; ENSMUSP00000073455; ENSMUSG00000060002. [Q8C025-2]
ENSMUST00000139109; ENSMUSP00000116413; ENSMUSG00000060002. [Q8C025-1]
GeneIDi212862.
KEGGimmu:212862.
UCSCiuc007grr.2. mouse. [Q8C025-1]
uc007grs.2. mouse. [Q8C025-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY445814 mRNA. Translation: AAR16089.1.
AK032497 mRNA. Translation: BAC27897.1.
BC016251 mRNA. Translation: AAH16251.1.
BC031435 mRNA. Translation: AAH31435.1.
CCDSiCCDS24112.1. [Q8C025-1]
PIRiS12207.
RefSeqiNP_659056.3. NM_144807.3. [Q8C025-1]
XP_006513518.1. XM_006513455.1. [Q8C025-2]
UniGeneiMm.288897.

3D structure databases

ProteinModelPortaliQ8C025.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112708.

PTM databases

iPTMnetiQ8C025.
PhosphoSiteiQ8C025.

Proteomic databases

MaxQBiQ8C025.
PaxDbiQ8C025.
PRIDEiQ8C025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020253; ENSMUSP00000020253; ENSMUSG00000060002. [Q8C025-1]
ENSMUST00000073783; ENSMUSP00000073455; ENSMUSG00000060002. [Q8C025-2]
ENSMUST00000139109; ENSMUSP00000116413; ENSMUSG00000060002. [Q8C025-1]
GeneIDi212862.
KEGGimmu:212862.
UCSCiuc007grr.2. mouse. [Q8C025-1]
uc007grs.2. mouse. [Q8C025-2]

Organism-specific databases

CTDi56994.
MGIiMGI:2384841. Chpt1.

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
GeneTreeiENSGT00530000063048.
HOGENOMiHOG000230699.
HOVERGENiHBG107647.
InParanoidiQ8C025.
KOiK00994.
PhylomeDBiQ8C025.

Enzyme and pathway databases

UniPathwayiUPA00753; UER00740.
ReactomeiR-MMU-1483191. Synthesis of PC.

Miscellaneous databases

ChiTaRSiChpt1. mouse.
PROiQ8C025.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060002.
CleanExiMM_CHPT1.
ExpressionAtlasiQ8C025. baseline and differential.
GenevisibleiQ8C025. MM.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHPT1_MOUSE
AccessioniPrimary (citable) accession number: Q8C025
Secondary accession number(s): Q6SXV1, Q8K0H2, Q91W91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.