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Protein

Serine/threonine-protein kinase PAK 7

Gene

Pak7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei478 – 4781ATPPROSITE-ProRule annotation
Active sitei568 – 5681Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi455 – 4639ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell growth Source: InterPro
  • cytoskeleton organization Source: InterPro
  • learning Source: MGI
  • locomotory behavior Source: MGI
  • memory Source: MGI
  • negative regulation of extrinsic apoptotic signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PAK 7 (EC:2.7.11.1)
Alternative name(s):
p21-activated kinase 5
Short name:
PAK-5
p21-activated kinase 7
Short name:
PAK-7
Gene namesi
Name:Pak7
Synonyms:Pak5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1920334. Pak7.

Subcellular locationi

  • Mitochondrion By similarity
  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.By similarity

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, and show normal development of brain, eye, pancreas and adrenal gland.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719Serine/threonine-protein kinase PAK 7PRO_0000086478Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei104 – 1041PhosphoserineCombined sources
Modified residuei107 – 1071PhosphothreonineCombined sources

Post-translational modificationi

Autophosphorylated when activated by CDC42/p21.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8C015.
PaxDbiQ8C015.
PRIDEiQ8C015.

PTM databases

iPTMnetiQ8C015.
PhosphoSiteiQ8C015.

Expressioni

Tissue specificityi

Highly expressed in brain and eye. Also expressed in adrenal gland, pancreas, prostate and testes. Within the brain, expression is restricted to neurons. Present in brain but not in kidney, lung and spleen (at protein level).1 Publication

Developmental stagei

Expressed in fetal brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000039913.
CleanExiMM_PAK7.
GenevisibleiQ8C015. MM.

Interactioni

Subunit structurei

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with MARK2, leading to inhibit MARK2 independently of kinase activity. Interacts with RHOD and RHOH (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047285.

Structurei

3D structure databases

ProteinModelPortaliQ8C015.
SMRiQ8C015. Positions 10-42, 401-718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 2414CRIBPROSITE-ProRule annotationAdd
BLAST
Domaini449 – 700252Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 448424LinkerAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 85Poly-Lys
Compositional biasi223 – 2264Poly-Ser
Compositional biasi360 – 42869Ser-richAdd
BLAST

Domaini

An autoinhibitory domain is present in the N-terminal region of the protein.By similarity

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0578. Eukaryota.
ENOG410XP4K. LUCA.
GeneTreeiENSGT00840000129718.
HOGENOMiHOG000234205.
HOVERGENiHBG108518.
InParanoidiQ8C015.
KOiK05736.
OMAiNQTSPQP.
OrthoDBiEOG091G0OT5.
PhylomeDBiQ8C015.
TreeFamiTF105352.

Family and domain databases

Gene3Di3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR028754. PAK7.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PANTHERiPTHR24361:SF183. PTHR24361:SF183. 2 hits.
PfamiPF00786. PBD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8C015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGKKKKKIE ISGPSNFEHR VHTGFDPQEQ KFTGLPQQWH SLLADTANRP
60 70 80 90 100
KPMVDPSCIT PIQLAPMKTI VRGNKSCKET SINGLLEDFD NISVTRSNSL
110 120 130 140 150
RKESPPTPDQ GAASRIQGHS EENGFITFSQ YSSESDTTAD YTTEKYRDRS
160 170 180 190 200
LYGDDLDLYY KSSHAAKQNG HAMKMKHGDA YYPEMKSLKT DLAGFPVDYH
210 220 230 240 250
THLDSLRKSS EYGDLRWDYQ RASSSSPLDY SFQLTPSRTA GTSRCSKESL
260 270 280 290 300
AYSESDWGPS LDDYDRRPKS SYLHQTSPQP AMRQRSKSGS GLQEPMMPFG
310 320 330 340 350
ASAFKTHPQG HSYNSYTYPR LSEPTMCIPK VDYDRAQMVF SPPLSGSDTY
360 370 380 390 400
PRGPTKLPQS QSKAGYSSGS HQYPSGYHKA SLYHHPSLQT SSQYISTASY
410 420 430 440 450
LSSLSISSST YPPPSWGSSS DQQPSRVSHE QFRAALQLVV SPGDPREYLD
460 470 480 490 500
NFIKIGEGST GIVCIATEKH TGKQVAVKKM DLRKQQRREL LFNEVVIMRD
510 520 530 540 550
YHHDNVVDMY NSYLVGDELW VVMEFLEGGA LTDIVTHTRM NEEQIATVCL
560 570 580 590 600
SVLKALSYLH NQGVIHRDIK SDSILLTSDG RIKLSDFGFC AQVSKEVPKR
610 620 630 640 650
KSLVGTPYWM APEVISRLPY GTEVDIWSLG IMVIEMIDGE PPYFNEPPLQ
660 670 680 690 700
AMRRIRDSLP PRVKDLHKVS SMLRGFLDLM LVREPSQRAT AQELLGHPFL
710
KLAGPPSCIV PLMRQYRHH
Length:719
Mass (Da):80,948
Last modified:March 1, 2003 - v1
Checksum:i5E16D2318C238C8D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti315 – 3151S → F in AAR37415 (PubMed:14517284).Curated
Sequence conflicti320 – 3201R → G in AAR37415 (PubMed:14517284).Curated
Sequence conflicti454 – 4541K → R in AAR37415 (PubMed:14517284).Curated
Sequence conflicti522 – 5221V → A in AAR37415 (PubMed:14517284).Curated
Sequence conflicti664 – 6641K → E in AAR37415 (PubMed:14517284).Curated
Sequence conflicti687 – 6871Q → R in AAR37415 (PubMed:14517284).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY487425 mRNA. Translation: AAR37415.1.
AK032593 mRNA. Translation: BAC27939.1.
AK163530 mRNA. Translation: BAE37385.1.
CCDSiCCDS16790.1.
RefSeqiNP_766446.2. NM_172858.2.
XP_006499572.1. XM_006499509.1.
XP_011237832.1. XM_011239530.1.
XP_011237833.1. XM_011239531.1.
UniGeneiMm.131572.

Genome annotation databases

EnsembliENSMUST00000035264; ENSMUSP00000047285; ENSMUSG00000039913.
ENSMUST00000077200; ENSMUSP00000076440; ENSMUSG00000039913.
GeneIDi241656.
KEGGimmu:241656.
UCSCiuc008moh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY487425 mRNA. Translation: AAR37415.1.
AK032593 mRNA. Translation: BAC27939.1.
AK163530 mRNA. Translation: BAE37385.1.
CCDSiCCDS16790.1.
RefSeqiNP_766446.2. NM_172858.2.
XP_006499572.1. XM_006499509.1.
XP_011237832.1. XM_011239530.1.
XP_011237833.1. XM_011239531.1.
UniGeneiMm.131572.

3D structure databases

ProteinModelPortaliQ8C015.
SMRiQ8C015. Positions 10-42, 401-718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047285.

PTM databases

iPTMnetiQ8C015.
PhosphoSiteiQ8C015.

Proteomic databases

MaxQBiQ8C015.
PaxDbiQ8C015.
PRIDEiQ8C015.

Protocols and materials databases

DNASUi241656.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035264; ENSMUSP00000047285; ENSMUSG00000039913.
ENSMUST00000077200; ENSMUSP00000076440; ENSMUSG00000039913.
GeneIDi241656.
KEGGimmu:241656.
UCSCiuc008moh.1. mouse.

Organism-specific databases

CTDi57144.
MGIiMGI:1920334. Pak7.

Phylogenomic databases

eggNOGiKOG0578. Eukaryota.
ENOG410XP4K. LUCA.
GeneTreeiENSGT00840000129718.
HOGENOMiHOG000234205.
HOVERGENiHBG108518.
InParanoidiQ8C015.
KOiK05736.
OMAiNQTSPQP.
OrthoDBiEOG091G0OT5.
PhylomeDBiQ8C015.
TreeFamiTF105352.

Miscellaneous databases

PROiQ8C015.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039913.
CleanExiMM_PAK7.
GenevisibleiQ8C015. MM.

Family and domain databases

Gene3Di3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR028754. PAK7.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PANTHERiPTHR24361:SF183. PTHR24361:SF183. 2 hits.
PfamiPF00786. PBD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAK7_MOUSE
AccessioniPrimary (citable) accession number: Q8C015
Secondary accession number(s): Q3TQJ7, Q6RWS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.