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Protein

Putative E3 ubiquitin-protein ligase SH3RF2

Gene

Sh3rf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits PPP1CA phosphatase activity. May be a E3 ubiquitin-protein ligase (Potential). May play a role in cardiac function (By similarity).By similarityCurated

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 5342RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase, Protein phosphatase inhibitor

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative E3 ubiquitin-protein ligase SH3RF2 (EC:6.3.2.-)
Alternative name(s):
Protein phosphatase 1 regulatory subunit 39
RING finger protein 158
SH3 domain-containing RING finger protein 2
Gene namesi
Name:Sh3rf2
Synonyms:Ppp1r39, Rnf158
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2444628. Sh3rf2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Putative E3 ubiquitin-protein ligase SH3RF2PRO_0000269513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei655 – 6551PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BZT2.
PaxDbiQ8BZT2.
PRIDEiQ8BZT2.

PTM databases

iPTMnetiQ8BZT2.
PhosphoSiteiQ8BZT2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000057719.
CleanExiMM_SH3RF2.
GenevisibleiQ8BZT2. MM.

Interactioni

Subunit structurei

Interacts with FASLG and PPP1CA.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071896.

Structurei

Secondary structure

1
735
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi129 – 1324Combined sources
Beta strandi151 – 1566Combined sources
Beta strandi160 – 1678Combined sources
Beta strandi170 – 18011Combined sources
Beta strandi408 – 4169Combined sources
Beta strandi419 – 4246Combined sources
Turni425 – 4273Combined sources
Beta strandi430 – 4345Combined sources
Helixi435 – 4373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CUCNMR-A385-441[»]
2DJQNMR-A128-182[»]
ProteinModelPortaliQ8BZT2.
SMRiQ8BZT2. Positions 5-78, 92-255, 383-441.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8BZT2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 18460SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini187 – 25266SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini382 – 44362SH3 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni647 – 6526Interaction with PPP1CABy similarity

Domaini

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

Sequence similaritiesi

Belongs to the SH3RF family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 3 SH3 domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri12 – 5342RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KDQA. Eukaryota.
ENOG410XS5R. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000060125.
HOVERGENiHBG082418.
InParanoidiQ8BZT2.
KOiK12171.
OMAiNMFVALH.
OrthoDBiEOG091G09B6.
PhylomeDBiQ8BZT2.
TreeFamiTF105571.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011511. SH3_2.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00018. SH3_1. 2 hits.
PF07653. SH3_2. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BZT2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDLTLLDLL ECPVCFEKLD VTAKVLPCQH TFCKPCLQRI FKAHKELRCP
60 70 80 90 100
ECRTLVFCSI EALPANLLLV RLLDGVRSGQ SSWKGGSFRR PRILTLQDNR
110 120 130 140 150
KAKSSPRSLQ ASPFRLVPSV RIHMDGVPRA KALCNYRGKN PGDLKFNKGD
160 170 180 190 200
VILLRRQLDE NWYQGEINGV SGIFPASSVE VIKQLPQPPP LCRALYNFDL
210 220 230 240 250
RDKDKSENQD CLTFLKDDVI TVISRVDENW AEGKLGDKVG IFPILFVEPN
260 270 280 290 300
VSARHLLENK GHQLSRTRHL SLMSSPSRGK ATNTSSLRKS PGSRRKGSGQ
310 320 330 340 350
FSMTTALNTL NRTVHSPEGH QMVEISTPML ISSNSPSVLT QHGDKADFPT
360 370 380 390 400
SSTGQVSSSQ PAPASPGHST AMVSVPSSQQ HLSNNMFVAL HTYSAHRPEE
410 420 430 440 450
LDLQKGEGIR VLGKYQDGWL KGLSLLTGRT GIFPSDYVIP VFSSTARKTS
460 470 480 490 500
SFPDSRSPTV CTTWALSTSS VSSQGSFAEG DPRQSGPFKS VFVPTAVVNP
510 520 530 540 550
SRSTPGPGSS GQGSLRKVRS SMRKNGSLQR PVQSGIPTFM VGSLRCSPTM
560 570 580 590 600
VIRPQRFQFY PPQGMTPSPT PIMVEMGSKS IYTGEPALTC INRGSKTRIH
610 620 630 640 650
SAGNSIIMEG KETPIKSEPP PKPPASAPPS ILVKPENSKN GTEKQVKTVR
660 670 680 690 700
FQNYSPPPTK HSASSPTSGK HDHPATLKGS QHEAVSSGGE MTILFAHRSG
710 720 730
CHSGQQTDLR RKSAFGKTMP LLSTASATQT LFPSK
Length:735
Mass (Da):80,066
Last modified:December 12, 2006 - v2
Checksum:iFAE1D97780289FCC
GO
Isoform 2 (identifier: Q8BZT2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-248: Missing.

Show »
Length:703
Mass (Da):76,509
Checksum:i3AB3F930053B3E1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1271V → G in BAC26334 (PubMed:16141072).Curated
Sequence conflicti258 – 2581E → Q in BAC25780 (PubMed:16141072).Curated
Sequence conflicti662 – 6621S → C in BAC28382 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei217 – 24832Missing in isoform 2. 1 PublicationVSP_022060Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028153 mRNA. Translation: BAC25780.1.
AK029168 mRNA. Translation: BAC26334.1.
AK033601 mRNA. Translation: BAC28382.1.
BC048400 mRNA. Translation: AAH48400.1.
BC137955 mRNA. Translation: AAI37956.1.
BC145060 mRNA. Translation: AAI45061.1.
CCDSiCCDS29210.1. [Q8BZT2-2]
CCDS50265.1. [Q8BZT2-1]
RefSeqiNP_001139771.1. NM_001146299.1. [Q8BZT2-1]
NP_766554.2. NM_172966.3. [Q8BZT2-2]
XP_006526018.1. XM_006525955.1. [Q8BZT2-1]
XP_006526019.1. XM_006525956.2. [Q8BZT2-1]
UniGeneiMm.45472.

Genome annotation databases

EnsembliENSMUST00000072008; ENSMUSP00000071896; ENSMUSG00000057719. [Q8BZT2-1]
ENSMUST00000074679; ENSMUSP00000074247; ENSMUSG00000057719. [Q8BZT2-2]
GeneIDi269016.
KEGGimmu:269016.
UCSCiuc008etk.2. mouse. [Q8BZT2-2]
uc008etl.2. mouse. [Q8BZT2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028153 mRNA. Translation: BAC25780.1.
AK029168 mRNA. Translation: BAC26334.1.
AK033601 mRNA. Translation: BAC28382.1.
BC048400 mRNA. Translation: AAH48400.1.
BC137955 mRNA. Translation: AAI37956.1.
BC145060 mRNA. Translation: AAI45061.1.
CCDSiCCDS29210.1. [Q8BZT2-2]
CCDS50265.1. [Q8BZT2-1]
RefSeqiNP_001139771.1. NM_001146299.1. [Q8BZT2-1]
NP_766554.2. NM_172966.3. [Q8BZT2-2]
XP_006526018.1. XM_006525955.1. [Q8BZT2-1]
XP_006526019.1. XM_006525956.2. [Q8BZT2-1]
UniGeneiMm.45472.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CUCNMR-A385-441[»]
2DJQNMR-A128-182[»]
ProteinModelPortaliQ8BZT2.
SMRiQ8BZT2. Positions 5-78, 92-255, 383-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071896.

PTM databases

iPTMnetiQ8BZT2.
PhosphoSiteiQ8BZT2.

Proteomic databases

MaxQBiQ8BZT2.
PaxDbiQ8BZT2.
PRIDEiQ8BZT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072008; ENSMUSP00000071896; ENSMUSG00000057719. [Q8BZT2-1]
ENSMUST00000074679; ENSMUSP00000074247; ENSMUSG00000057719. [Q8BZT2-2]
GeneIDi269016.
KEGGimmu:269016.
UCSCiuc008etk.2. mouse. [Q8BZT2-2]
uc008etl.2. mouse. [Q8BZT2-1]

Organism-specific databases

CTDi153769.
MGIiMGI:2444628. Sh3rf2.

Phylogenomic databases

eggNOGiENOG410KDQA. Eukaryota.
ENOG410XS5R. LUCA.
GeneTreeiENSGT00760000119190.
HOGENOMiHOG000060125.
HOVERGENiHBG082418.
InParanoidiQ8BZT2.
KOiK12171.
OMAiNMFVALH.
OrthoDBiEOG091G09B6.
PhylomeDBiQ8BZT2.
TreeFamiTF105571.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

EvolutionaryTraceiQ8BZT2.
PROiQ8BZT2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057719.
CleanExiMM_SH3RF2.
GenevisibleiQ8BZT2. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR011511. SH3_2.
IPR001452. SH3_domain.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00018. SH3_1. 2 hits.
PF07653. SH3_2. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00184. RING. 1 hit.
SM00326. SH3. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 3 hits.
PROSITEiPS50002. SH3. 3 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSH3R2_MOUSE
AccessioniPrimary (citable) accession number: Q8BZT2
Secondary accession number(s): B2RQJ2
, Q80VJ1, Q8C122, Q8C1E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: September 7, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.