Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Gene

Dhx32

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi85 – 928ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 32
MuDDX32
Gene namesi
Name:Dhx32
Synonyms:Ddx32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2141813. Dhx32.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32PRO_0000292664Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8BZS9.
MaxQBiQ8BZS9.
PaxDbiQ8BZS9.
PRIDEiQ8BZS9.

PTM databases

iPTMnetiQ8BZS9.
PhosphoSiteiQ8BZS9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030986.
CleanExiMM_DHX32.
ExpressionAtlasiQ8BZS9. baseline and differential.
GenevisibleiQ8BZS9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033290.

Structurei

3D structure databases

ProteinModelPortaliQ8BZS9.
SMRiQ8BZS9. Positions 3-713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 270199Helicase ATP-bindingAdd
BLAST
Domaini258 – 438181Helicase C-terminalAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi185 – 1884DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.Curated
Contains 1 helicase C-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00770000120558.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ8BZS9.
KOiK18994.
OMAiSGDAKCG.
OrthoDBiEOG091G025L.
PhylomeDBiQ8BZS9.
TreeFamiTF105735.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00847. HA2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BZS9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEEELDHPN ASPEKRYFPE SLDSSDGDEE GVLACEDLEL NPFDGLPYSS
60 70 80 90 100
RYYKLLKERE ELPIWKEKYS FMESLLQNQV VVVSGDSKCG KSSQVPQWCA
110 120 130 140 150
EYCLSIHYQH GGVICTQAHK QTAVQLALRV ADEMDVNIGH EVGYVIPFEN
160 170 180 190 200
CCTTETILRY CTDDMLQREM MSNPFLGSYG VIILDDVHER SLATDVLLGL
210 220 230 240 250
LKDVLLARPE LKLIVNCSPL LTSKLSSYYG DVPVIEVRNK HPVEVVYLSG
260 270 280 290 300
AQKDSFESVI RLIFEIHRSG EKGDVVVFLA CEQDIEKTYE LVCQEGSNLN
310 320 330 340 350
PDVGDLVVIP LYPKEKCSLF RPVDETEKRC QVYQRRVVLT TSCGESLIWS
360 370 380 390 400
HTVKFVIDVG LERRQVYNPR IRANSLVLQP ISQSQAEIRK QLLGSSPSGK
410 420 430 440 450
LFCLYTEEFA SKDMRPLKPA EMQEANLTSM VLFMKRVDIA GLGRCDFMNR
460 470 480 490 500
PAPESLMQAL EDLDYLAALD NDGNLSEFGI IMSEFPLDPQ LSKSILASCE
510 520 530 540 550
FDCVDEMLTI AAMVTAPSCF LHVPHGAEEA AVTCWKTFLH PEGDHFTLIN
560 570 580 590 600
VYNAYQDTVL NSANEHCVEM WCHDCFLSCS ALRMADVIRA ELLEIIKRIE
610 620 630 640 650
LPYAEPAFGS KENGLNIKKA LLSGYFMQIA RDVDGSGNYL MLTHKQVAQL
660 670 680 690 700
HPLSSYSITK KMPEWVLFHQ FSISENNYIR VASAVSPELF MQLVPQYYFS
710 720 730 740
NLPPSESKDI LQQAAGHLPT ETVNKDQDVC DKCPDATEQR CTIQ
Length:744
Mass (Da):83,941
Last modified:June 26, 2007 - v2
Checksum:i8B287F5726EFB569
GO
Isoform 2 (identifier: Q8BZS9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSSLREEM

Show »
Length:751
Mass (Da):84,774
Checksum:i2D2992939344DC8C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti462 – 4621D → N in BAC28397 (PubMed:16141072).Curated
Sequence conflicti479 – 4791G → E in BAE40804 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MSSLREEM in isoform 2. 2 PublicationsVSP_026428

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065980 mRNA. Translation: AAL47579.1.
AK033624 mRNA. Translation: BAC28397.1.
AK169005 mRNA. Translation: BAE40804.1.
AK172504 mRNA. Translation: BAE43038.1.
BC006911 mRNA. Translation: AAH06911.1.
BC022920 mRNA. Translation: AAH22920.1.
CCDSiCCDS21936.1. [Q8BZS9-2]
RefSeqiNP_001272959.1. NM_001286030.1. [Q8BZS9-2]
NP_001272960.1. NM_001286031.1. [Q8BZS9-2]
NP_001272961.1. NM_001286032.1.
NP_598702.1. NM_133941.2. [Q8BZS9-2]
XP_006507200.1. XM_006507137.1. [Q8BZS9-2]
XP_006507201.1. XM_006507138.1. [Q8BZS9-2]
UniGeneiMm.199223.

Genome annotation databases

EnsembliENSMUST00000033290; ENSMUSP00000033290; ENSMUSG00000030986. [Q8BZS9-2]
ENSMUST00000063669; ENSMUSP00000066067; ENSMUSG00000030986. [Q8BZS9-2]
GeneIDi101437.
KEGGimmu:101437.
UCSCiuc009kdj.1. mouse. [Q8BZS9-2]
uc012fvg.1. mouse. [Q8BZS9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065980 mRNA. Translation: AAL47579.1.
AK033624 mRNA. Translation: BAC28397.1.
AK169005 mRNA. Translation: BAE40804.1.
AK172504 mRNA. Translation: BAE43038.1.
BC006911 mRNA. Translation: AAH06911.1.
BC022920 mRNA. Translation: AAH22920.1.
CCDSiCCDS21936.1. [Q8BZS9-2]
RefSeqiNP_001272959.1. NM_001286030.1. [Q8BZS9-2]
NP_001272960.1. NM_001286031.1. [Q8BZS9-2]
NP_001272961.1. NM_001286032.1.
NP_598702.1. NM_133941.2. [Q8BZS9-2]
XP_006507200.1. XM_006507137.1. [Q8BZS9-2]
XP_006507201.1. XM_006507138.1. [Q8BZS9-2]
UniGeneiMm.199223.

3D structure databases

ProteinModelPortaliQ8BZS9.
SMRiQ8BZS9. Positions 3-713.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033290.

PTM databases

iPTMnetiQ8BZS9.
PhosphoSiteiQ8BZS9.

Proteomic databases

EPDiQ8BZS9.
MaxQBiQ8BZS9.
PaxDbiQ8BZS9.
PRIDEiQ8BZS9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033290; ENSMUSP00000033290; ENSMUSG00000030986. [Q8BZS9-2]
ENSMUST00000063669; ENSMUSP00000066067; ENSMUSG00000030986. [Q8BZS9-2]
GeneIDi101437.
KEGGimmu:101437.
UCSCiuc009kdj.1. mouse. [Q8BZS9-2]
uc012fvg.1. mouse. [Q8BZS9-1]

Organism-specific databases

CTDi55760.
MGIiMGI:2141813. Dhx32.

Phylogenomic databases

eggNOGiKOG0925. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00770000120558.
HOGENOMiHOG000175261.
HOVERGENiHBG039428.
InParanoidiQ8BZS9.
KOiK18994.
OMAiSGDAKCG.
OrthoDBiEOG091G025L.
PhylomeDBiQ8BZS9.
TreeFamiTF105735.

Miscellaneous databases

ChiTaRSiDhx32. mouse.
PROiQ8BZS9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030986.
CleanExiMM_DHX32.
ExpressionAtlasiQ8BZS9. baseline and differential.
GenevisibleiQ8BZS9. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF04408. HA2. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00847. HA2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDHX32_MOUSE
AccessioniPrimary (citable) accession number: Q8BZS9
Secondary accession number(s): Q3TFU4, Q8VH39, Q922N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.