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Protein

Dedicator of cytokinesis protein 10

Gene

Dock10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP.

GO - Molecular functioni

  • Rho GTPase binding Source: MGI
  • Rho guanyl-nucleotide exchange factor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 10
Alternative name(s):
Zizimin-3
Gene namesi
Name:Dock10
Synonyms:Kiaa0694, Ziz3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2146320. Dock10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21502150Dedicator of cytokinesis protein 10PRO_0000190003Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei196 – 1961PhosphothreonineCombined sources
Modified residuei302 – 3021PhosphoserineCombined sources
Modified residuei368 – 3681PhosphothreonineCombined sources
Modified residuei834 – 8341N6-acetyllysineBy similarity
Modified residuei877 – 8771PhosphoserineCombined sources
Modified residuei1232 – 12321PhosphoserineBy similarity
Modified residuei1251 – 12511PhosphoserineCombined sources
Modified residuei1257 – 12571PhosphoserineCombined sources
Modified residuei1292 – 12921PhosphoserineCombined sources
Modified residuei1295 – 12951PhosphoserineCombined sources
Modified residuei1318 – 13181PhosphoserineCombined sources
Modified residuei1406 – 14061PhosphothreonineCombined sources
Modified residuei1440 – 14401PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BZN6.
MaxQBiQ8BZN6.
PaxDbiQ8BZN6.
PRIDEiQ8BZN6.

PTM databases

iPTMnetiQ8BZN6.
PhosphoSiteiQ8BZN6.

Expressioni

Tissue specificityi

Expressed in brain, lung, spleen and thymus.1 Publication

Gene expression databases

CleanExiMM_DOCK10.

Interactioni

GO - Molecular functioni

  • Rho GTPase binding Source: MGI

Protein-protein interaction databases

BioGridi229145. 1 interaction.
STRINGi10090.ENSMUSP00000077099.

Structurei

3D structure databases

ProteinModelPortaliQ8BZN6.
SMRiQ8BZN6. Positions 1694-2150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini181 – 290110PHPROSITE-ProRule annotationAdd
BLAST
Domaini672 – 850179DHR-1Add
BLAST
Domaini1690 – 2150461DHR-2Add
BLAST

Domaini

The DHR-2 domain may mediate some GEF activity.By similarity

Sequence similaritiesi

Belongs to the DOCK family.Curated
Contains 1 DHR-1 domain.Curated
Contains 1 DHR-2 domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1997. Eukaryota.
ENOG410XNVY. LUCA.
HOGENOMiHOG000231373.
HOVERGENiHBG107819.
InParanoidiQ8BZN6.
PhylomeDBiQ8BZN6.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 5 hits.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BZN6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGERTRRFT RSLLRPGQAA ELRHSAASAA AVAVSSRQQQ RQEKPRLLDP
60 70 80 90 100
LDYETVIEEL EKTYRDDPLQ DLLFFPSDDF STATVSWDIR TLYSTVPEEA
110 120 130 140 150
EHRAESLLVK EACKFYSSQW YVVNYKYEQY SGDIRQLPRA EHKPEKLPSH
160 170 180 190 200
SFEVDHEDAD KDEDTTSHSS SKGGGGAGGT GVFKSGWLYK GNFNSTVNNT
210 220 230 240 250
VTVRSFKKRY FQLTQLPDNS YIMNFYKDEK ISKEPKGCIF LDSCTGVVQN
260 270 280 290 300
NRLRKYAFEL KMNDLTYFVL AAETESDMDE WIHTLNRILQ ISPEGPLQGR
310 320 330 340 350
KSAELAELGL DPLDNCVTCE CTLEETDSSE NSLHPDFAKY LTETEDTVKT
360 370 380 390 400
TRNMGRLNLF SLDPDIDTLK LQKRDSFENE LMIKPFEEKA AKRIMIICRA
410 420 430 440 450
LNFNLQGCVT ENEYDPVTNI EPFFVSVALY DLRDNRKISA DFHVDLNHPA
460 470 480 490 500
VRQMLSGTPP ALENGNIDTG TPRQSEEPHI KGLPEEWLKF PKQAVFSVSD
510 520 530 540 550
PHSEIVLVAK VEKVLMGNIG SGAEPYIKNP DSNKFAQKIL KSNRQFCSKL
560 570 580 590 600
GKYRMPFAWA VRSVFKDNQG NVDRDSRFSP LYRQESSKMS SEDLLKLVSD
610 620 630 640 650
YRRADRISKM QSIPGSLDIA VDNIPLEHPN CVTSSFIPVK PFNVSAQSEP
660 670 680 690 700
TVEVEEFIYD STKYCRPYRV YKNQIYVYPK HLKYDSQKCF NKARNITVCI
710 720 730 740 750
EFKNSDDDGA KPMKCIYGKP GGPLFTSSAY TAVLHHSQNP DFSDEVKIEL
760 770 780 790 800
PTQLHGKHHL LFSFYHITCD INAKANAKKK EALETSVGYA WLPLMKHDQI
810 820 830 840 850
ASQEYNIPIA TTLPPNYLSI QDPTSAKHGG SDIKWVDGGK PLFKVSTFVV
860 870 880 890 900
STVNTQDPHV NAFFRQCQKR EKDMSQSPTS SFVRACKNLL NVDKIHSIMS
910 920 930 940 950
FLPIILNQLF KILVQNEEDE ITATVTRVLA DIVAKCHEEQ LDHSVQSYIK
960 970 980 990 1000
FVFKTKSYKE RTIHEELAKN LSDLLKSNDS TIVKHVLEHS WFFFAIILKS
1010 1020 1030 1040 1050
MAQHLIDTNK IQLPRAQRFP ESYQSELDNL VMGLCDHVIW KCKEAPEETK
1060 1070 1080 1090 1100
RANHSVARFL KRCFTFMDRG FVFKMVNNYI SMFSSGEFKT LCQYKFDFLQ
1110 1120 1130 1140 1150
EVCQHEHFIP LCLPIRSANI PDPLTPSESI RELHASDMPE YSVTNEFCRK
1160 1170 1180 1190 1200
HFLIGILLRE VGFALQEDQD IRHLALAVLK NLMAKHSFDD RYREPRKQAQ
1210 1220 1230 1240 1250
IASLYMPLYG MLLDNMPRIY LKDLYPFTVN TSNQGSRDDL STNGGFQTQT
1260 1270 1280 1290 1300
SMKHATSVDT SFSKDVLNSI AAFSSIAIST VNHADSRASL ASLDSNPSTT
1310 1320 1330 1340 1350
EKSSEKTDNC EKIPRPLSLI GSTLRFDKLD QAETRSLLMC FLHIMKTISD
1360 1370 1380 1390 1400
ETLIAYWQRA PSPEVSDFFS ILDVCLQNFR YLGKRNIIRK IAAAFKFVQS
1410 1420 1430 1440 1450
TQNNGTLKGS NPSCQTSGLL SQWMHTTSGH EGHKQHRSQT LPIIRGKNAL
1460 1470 1480 1490 1500
SNPKLLQMLD NSMNSNSNEI DIVHHVDTEA NIATEVCLTI LDLLSLFTQV
1510 1520 1530 1540 1550
HQRQLQQSDC QNSLMKRVFD TYMLFFQVNQ SASALKHVFA SLRLFVCKFP
1560 1570 1580 1590 1600
SAFFQGPADL CGSFCYEVLK CCNHRSRLTQ MEASALLYFF MRKNFEFNKQ
1610 1620 1630 1640 1650
KSIVRSHLQL IKAVSQLIAD AGIGGSRFQH SLAITNNFAN GDKQMKNSNF
1660 1670 1680 1690 1700
PAEVKDLTKR IRTVLMATAQ MKEHEKDPEM LVDLQYSLAN SYASTPELRR
1710 1720 1730 1740 1750
TWLESMAKIH ARNGDLSEAA MCYIHIAALI AEYLKRKGYW KMEKICTPPL
1760 1770 1780 1790 1800
LPEDTQPCDS NLLLTTPGGG SMFSMGWPAF LSITPNIKEE GAMKEDSGMQ
1810 1820 1830 1840 1850
DTPYNENILV EQLYMCVEFL WKSERYELIA DVNKPIIAVF EKQRDFKKLS
1860 1870 1880 1890 1900
DLYYDIHRSY LKVAEVVNSE KRLFGRYYRV AFYGQAVGFF EEEEGKEYIY
1910 1920 1930 1940 1950
KEPKLTGLSE ISQRLLKLYA DKFGADNVKI IQDSNKVNPK DLDPKYAYIQ
1960 1970 1980 1990 2000
VTYVTPFFEE KEIEDRKTDF EMHHNINRFV FETPFTLSGK KHGGVAEQCK
2010 2020 2030 2040 2050
RRTVLTTSHL FPYVKKRIQV ISQSSTELNP IEVAIDEMSR KVSELNQLCT
2060 2070 2080 2090 2100
TEEVDMIRLQ LKLQGSVSVK VNAGPMAYAR AFLEETNAKK YPDNQVKLLK
2110 2120 2130 2140 2150
EIFRQFADAC GQALDVNERL IKEDQLEYQE ELRSHYKDML SELSAIMNEQ
Length:2,150
Mass (Da):245,758
Last modified:January 9, 2007 - v3
Checksum:iD7CDA1FE6476AB0E
GO
Isoform 2 (identifier: Q8BZN6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1739-1771: Missing.
     2150-2150: Q → LCRGPCLYSF...KFLQCSCLPC

Note: No experimental confirmation available.
Show »
Length:2,222
Mass (Da):253,466
Checksum:i83B60277F1092B94
GO
Isoform 3 (identifier: Q8BZN6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1739-1771: Missing.
     2150-2150: Q → LCRGPCLYSF...RKWLFYKGNL

Note: No experimental confirmation available.
Show »
Length:2,251
Mass (Da):257,396
Checksum:iE767B4733602DE79
GO

Sequence cautioni

The sequence BAC28567.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC65629.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1739 – 177133Missing in isoform 2 and isoform 3. 2 PublicationsVSP_007718Add
BLAST
Alternative sequencei2150 – 21501Q → LCRGPCLYSFCASVSSISLS TVSKSDYGQGRPSKVRSGAT LHHTCNQGDRGCTCGLHLIQ CGGLRGTLEHPMHLSENSLN VLQLISGKKKIDLIYLKFLQ CSCLPC in isoform 2. 1 PublicationVSP_022288
Alternative sequencei2150 – 21501Q → LCRGPCLYSFCASVSSISLS TVSKSGTSFSLYVYPVLQPP VHPPLLITSPVPQSALVAQL LLRLHCHLKTRHVDSLAFKK KTHAPSSPKCIFELFQFFKT KTQNVFMILQNIKEKHRPGV EEERNRKWLFYKGNL in isoform 3. 1 PublicationVSP_022227

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC124672 Genomic DNA. No translation available.
AC132387 Genomic DNA. No translation available.
AK122347 mRNA. Translation: BAC65629.1. Different initiation.
AK034064 mRNA. Translation: BAC28567.1. Different initiation.
RefSeqiNP_001272856.1. NM_001285927.1.
NP_780500.2. NM_175291.4.
UniGeneiMm.133473.

Genome annotation databases

GeneIDi210293.
KEGGimmu:210293.
UCSCiuc007brf.1. mouse. [Q8BZN6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC124672 Genomic DNA. No translation available.
AC132387 Genomic DNA. No translation available.
AK122347 mRNA. Translation: BAC65629.1. Different initiation.
AK034064 mRNA. Translation: BAC28567.1. Different initiation.
RefSeqiNP_001272856.1. NM_001285927.1.
NP_780500.2. NM_175291.4.
UniGeneiMm.133473.

3D structure databases

ProteinModelPortaliQ8BZN6.
SMRiQ8BZN6. Positions 1694-2150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229145. 1 interaction.
STRINGi10090.ENSMUSP00000077099.

PTM databases

iPTMnetiQ8BZN6.
PhosphoSiteiQ8BZN6.

Proteomic databases

EPDiQ8BZN6.
MaxQBiQ8BZN6.
PaxDbiQ8BZN6.
PRIDEiQ8BZN6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi210293.
KEGGimmu:210293.
UCSCiuc007brf.1. mouse. [Q8BZN6-2]

Organism-specific databases

CTDi55619.
MGIiMGI:2146320. Dock10.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1997. Eukaryota.
ENOG410XNVY. LUCA.
HOGENOMiHOG000231373.
HOVERGENiHBG107819.
InParanoidiQ8BZN6.
PhylomeDBiQ8BZN6.

Miscellaneous databases

ChiTaRSiDock10. mouse.
PROiQ8BZN6.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DOCK10.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 5 hits.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 5 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 348-2150 (ISOFORM 2).
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1690-2150 (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Diencephalon.
  4. "Zizimin2: a novel, DOCK180-related Cdc42 guanine nucleotide exchange factor expressed predominantly in lymphocytes."
    Nishikimi A., Meller N., Uekawa N., Isobe K., Schwartz M.A., Maruyama M.
    FEBS Lett. 579:1039-1046(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-877; SER-1251; SER-1257; SER-1292; SER-1295 AND THR-1440, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-196; SER-302; THR-368; SER-1257; SER-1292; SER-1295; SER-1318 AND THR-1406, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung and Spleen.

Entry informationi

Entry nameiDOC10_MOUSE
AccessioniPrimary (citable) accession number: Q8BZN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: January 9, 2007
Last modified: March 16, 2016
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.